FastQCFastQC Report
Thu 26 May 2016
SRR1512885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1403493
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77180.5499136796549752No Hit
TATCAACGCAGAGTACTTTTTTTTT57380.40883709430684734No Hit
GGTATCAACGCAGAGTACTTTTTTT46250.329534953149036No Hit
GTACTGGTTCACTATCGGTCAGTCA34960.24909279918033078No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26180.1865345961825246No Hit
GGATACCACGTGTCCCGCCCTACTC22640.16131181274149567No Hit
ATCATTAACTGAATCCATAGGTTAA20460.14577913819306543No Hit
GTCCTACAGTGGACATTTCTAAATT18090.12889269843169862No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17790.12675517441127246No Hit
CCCATGTACTCTGCGTTGATACCAC17390.12390514238403755No Hit
GTACATGGGAAGCAGTGGTATCAAC17360.12369138998199493No Hit
TATTCAGACAGGATACCACGTGTCC16480.11742131952207813No Hit
CTGTAGGACGTGGAATATGGCAAGA16000.11400128108939624No Hit
GTCCTAAAGTGTGTATTTCTCATTT15910.1133600238832684No Hit
CCCATATTCAGACAGGATACCACGT15910.1133600238832684No Hit
CTATCGGTCAGTCAGGAGTATTTAG15040.10716120422403247No Hit
GTATTTAGCCTTGGAGGATGGTCCC14930.10637744541654287No Hit
CTTTAGGACGTGAAATATGGCGAGG14450.10295740698386098No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCAC500.001499653113.2997268
ACTTATT802.8664985E-511.8747558
AGGACGA1052.6908674E-711.7713155
GTAAGAC657.966534E-411.702083
TATTCTA657.968919E-411.7016625
GGACCGT657.968919E-411.7016626
TCGAACT2050.011.57728519
GCCTCGA2250.011.39367116
CTCGAAC2250.011.39285918
CCGTACA2800.011.18941617
GGTATCA17100.011.1215871
GTAATAC600.005838754611.0925953
CCTAGAC951.3472883E-511.0091923
TACTAAG700.00148088810.8662162
CTAGACA1053.4317782E-610.86582854
GGCGTGT700.00149150210.8569198
CCCGTAC2800.010.85111516
GGCGAGG4850.010.7656819
TAGAAAT2500.010.6485124
TTAGGGT909.43582E-510.5639994
Produced by FastQC (version 0.11.5)