##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512885_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1403493
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.106576235150442	31.0	30.0	34.0	26.0	34.0
2	30.106434446057087	31.0	30.0	34.0	26.0	34.0
3	30.304129055150256	31.0	30.0	34.0	26.0	34.0
4	33.900719134331275	37.0	35.0	37.0	30.0	37.0
5	33.461408785081225	37.0	35.0	37.0	28.0	37.0
6	33.29711797636326	37.0	35.0	37.0	27.0	37.0
7	33.13127960025451	37.0	35.0	37.0	26.0	37.0
8	33.06871355966862	37.0	35.0	37.0	26.0	37.0
9	34.35553365780948	39.0	35.0	39.0	25.0	39.0
10	34.29685506090875	39.0	35.0	39.0	25.0	39.0
11	34.27212462050042	39.0	35.0	39.0	25.0	39.0
12	34.122932568954745	39.0	35.0	39.0	25.0	39.0
13	34.11113984893405	39.0	35.0	39.0	25.0	39.0
14	35.160064211221574	39.0	35.0	41.0	25.0	41.0
15	35.158793809445434	39.0	35.0	41.0	25.0	41.0
16	35.15449524864036	39.0	35.0	41.0	25.0	41.0
17	35.15261494001039	39.0	35.0	41.0	25.0	41.0
18	35.08024051420278	39.0	35.0	41.0	25.0	41.0
19	35.08634172026508	40.0	35.0	41.0	24.0	41.0
20	35.06380509200972	40.0	35.0	41.0	24.0	41.0
21	35.024313623224344	40.0	35.0	41.0	24.0	41.0
22	34.983920831810345	40.0	35.0	41.0	24.0	41.0
23	34.82153883204263	39.0	35.0	41.0	22.0	41.0
24	34.76643631282806	39.0	35.0	41.0	22.0	41.0
25	34.70125109280916	39.0	35.0	41.0	21.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7798875986132501
1101	2	0.6488296869880301
1101	3	0.7302359774139973
1101	4	0.7229596802879072
1101	5	0.8040931593523908
1101	6	0.9245868584620283
1101	7	1.0202501665605865
1101	8	0.7374995234823842
1101	9	1.149648255606273
1101	10	0.8869422664673934
1101	11	1.1711568273964588
1101	12	1.228949873557582
1101	13	1.2783091295607036
1101	14	1.4512938640031976
1101	15	1.1837086630016458
1101	16	1.394964935429769
1101	17	1.4923089326376129
1101	18	1.2020050892104024
1101	19	1.0807787506975757
1101	20	1.1932106493799424
1101	21	1.2796546305808363
1101	22	1.0492916646837713
1101	23	1.2236230837372517
1101	24	1.1248902787777553
1101	25	1.2804624166430827
1102	1	0.9915918066715932
1102	2	1.0748226021088492
1102	3	0.819785339580811
1102	4	0.8914114595346447
1102	5	1.2053855852707756
1102	6	1.2189906242446682
1102	7	1.1124144595457466
1102	8	1.2862901014398744
1102	9	1.5223078726770183
1102	10	1.3693209229160246
1102	11	1.5799273203565676
1102	12	1.5220885138464766
1102	13	1.4364586370945602
1102	14	1.46890197633099
1102	15	1.653885264553054
1102	16	1.6049592283619987
1102	17	1.659112542468236
1102	18	1.128029979788181
1102	19	1.4699868796085056
1102	20	1.6756274558565352
1102	21	1.5646214466721204
1102	22	1.2239896840578126
1102	23	1.530711403036669
1102	24	1.5633476680632867
1102	25	1.5490026686714273
1103	1	0.766611240731315
1103	2	0.9622691371353334
1103	3	0.7115227104252888
1103	4	0.6134453787878371
1103	5	0.7509047559478432
1103	6	0.9250643219495771
1103	7	0.9782998429166696
1103	8	1.0400111272172765
1103	9	1.0532255012444054
1103	10	0.9406413649897445
1103	11	1.015311415794848
1103	12	1.1622038226008442
1103	13	1.156525710171742
1103	14	1.1796720253764192
1103	15	1.2539090883236312
1103	16	1.2026935835177355
1103	17	1.1374770214393877
1103	18	1.1438207925921589
1103	19	1.1890660546497145
1103	20	0.9714302529973438
1103	21	1.1232873751854626
1103	22	1.1253908115742277
1103	23	1.1275693076699866
1103	24	1.135860790315455
1103	25	1.210245911500003
1104	1	0.6559569625292525
1104	2	0.8324282192409349
1104	3	0.616866443054743
1104	4	0.5771633977157578
1104	5	0.6852800738192286
1104	6	0.46620425111627384
1104	7	0.5010068792919427
1104	8	0.6294576431414143
1104	9	0.6699499284594381
1104	10	0.617110508732857
1104	11	0.7348070136293643
1104	12	0.7588078516944847
1104	13	0.6585583403436033
1104	14	0.977538190093135
1104	15	0.8237622946762357
1104	16	0.8280400697481909
1104	17	0.9087323038838448
1104	18	0.32192185591367917
1104	19	0.7765963519667025
1104	20	0.9023855573267667
1104	21	0.7728791127385222
1104	22	0.49399585580137284
1104	23	0.6080488452294119
1104	24	0.813764683475739
1104	25	0.6949920921629484
1105	1	0.47650006261136824
1105	2	0.5462639086454644
1105	3	0.5158226696867771
1105	4	0.3820996596450783
1105	5	0.3079348291130515
1105	6	0.380773291506209
1105	7	0.4564991300654242
1105	8	0.3393228520351741
1105	9	0.6865672620414074
1105	10	0.38589023865610983
1105	11	0.6103695704877623
1105	12	0.5779229882113057
1105	13	0.6534065445197328
1105	14	0.705569856679098
1105	15	0.7019199125939792
1105	16	0.6268313490737327
1105	17	0.3286636256361817
1105	18	0.274583936475139
1105	19	0.5997796392294958
1105	20	0.6493450561177312
1105	21	0.40354818475795895
1105	22	0.5064253204798774
1105	23	0.7509890283920129
1105	24	0.6318965238693863
1105	25	0.4456463694083581
1106	1	0.0754430405507236
1106	2	0.2476456898773165
1106	3	-1.62049586203495
1106	4	-0.3497656343404998
1106	5	0.016839512665221434
1106	6	0.05336819212315902
1106	7	0.3085160415751389
1106	8	0.24380971462547052
1106	9	0.5022522551319781
1106	10	0.4382001818100747
1106	11	0.3434651757509499
1106	12	0.4036897455631845
1106	13	0.4003524274778343
1106	14	0.4771122677392512
1106	15	0.5706862177495466
1106	16	0.5542830383800776
1106	17	0.5749269978401301
1106	18	0.4883985865816527
1106	19	0.4121569585802263
1106	20	0.617000897830053
1106	21	0.5531292443730322
1106	22	0.5602708337753981
1106	23	0.5402870489527132
1106	24	0.6421007711417133
1106	25	0.6170509080258171
1107	1	0.6836383556282541
1107	2	0.7376875648614529
1107	3	0.6865223853066205
1107	4	0.5239597165211052
1107	5	0.6204047234359749
1107	6	0.6415660450785197
1107	7	0.7147612513307422
1107	8	0.7563645962334675
1107	9	0.7281822604615229
1107	10	0.8267848073462076
1107	11	0.7568316706878804
1107	12	0.9741031658611305
1107	13	0.7417315102887869
1107	14	0.9391674412031961
1107	15	0.7314069750522876
1107	16	0.9012799675479073
1107	17	0.8396449127197059
1107	18	0.8591937037047117
1107	19	0.9673715361504662
1107	20	1.1155852695783963
1107	21	1.0060703909862312
1107	22	0.9328607380974034
1107	23	1.1034533053291327
1107	24	1.1325263732532278
1107	25	1.139955255410534
1108	1	0.5384331884550022
1108	2	0.44620677614662085
1108	3	0.42155141655905837
1108	4	0.389733038943362
1108	5	0.37577764838336947
1108	6	0.47544532183013644
1108	7	0.4826332020750357
1108	8	0.49666543015132447
1108	9	0.7491137934675223
1108	10	0.6910198630706219
1108	11	0.6139688774527485
1108	12	0.6488615634918702
1108	13	0.8859665660132023
1108	14	0.7988884294348253
1108	15	0.7053784114173425
1108	16	0.5925528613208044
1108	17	0.6943992186974128
1108	18	0.8841709073521287
1108	19	0.7890561281542077
1108	20	0.8078391858748617
1108	21	0.8775696895599978
1108	22	1.0042361180366157
1108	23	0.9710353332303114
1108	24	0.9664574776098647
1108	25	0.975022670265183
1109	1	0.19848844381167652
1109	2	0.15437593821649287
1109	3	0.2465237419746451
1109	4	0.14301411890884452
1109	5	-0.1041184077538233
1109	6	0.15555057906305336
1109	7	0.20198008188290828
1109	8	0.23252793906348046
1109	9	0.33833149394408935
1109	10	0.09442149164955538
1109	11	0.25385921104875564
1109	12	0.39334071843201457
1109	13	0.3149413868319968
1109	14	0.47661554865130284
1109	15	0.49038676069874043
1109	16	0.28512340805801273
1109	17	0.49357789331935464
1109	18	0.5940967019410479
1109	19	0.5761166182288804
1109	20	0.5557846997778242
1109	21	0.625297090212932
1109	22	0.6068642654342682
1109	23	0.5380781866206803
1109	24	0.3810978018246587
1109	25	0.5426143247362702
1110	1	0.20656426997648936
1110	2	0.01486841464624078
1110	3	0.030452940528206796
1110	4	0.09231521222889683
1110	5	0.18271698447909301
1110	6	0.07412814381129351
1110	7	0.042731049585000846
1110	8	0.047619074796486416
1110	9	0.22044955552969014
1110	10	0.38720994020022204
1110	11	0.2301930576597755
1110	12	0.3635181272673407
1110	13	0.14889624926971834
1110	14	0.19897843983257957
1110	15	0.3107966881837996
1110	16	0.27467357612751186
1110	17	0.26232700087225425
1110	18	0.13111617947838994
1110	19	0.2295573153603101
1110	20	0.4022546614556646
1110	21	0.25920815038220013
1110	22	0.2919479528520128
1110	23	0.4511061776209573
1110	24	0.5353022407118075
1110	25	0.556191098342218
1111	1	0.12732089531079538
1111	2	0.11395618513007477
1111	3	-0.08179019100595397
1111	4	0.17185715241853927
1111	5	0.302089705120963
1111	6	0.23800560551807592
1111	7	0.175415707985195
1111	8	0.251568503437376
1111	9	0.17659251687177857
1111	10	0.11903877560017406
1111	11	0.1990197048759157
1111	12	0.3474218471798096
1111	13	0.3166521452094173
1111	14	0.4042028502386046
1111	15	0.5321786478239616
1111	16	0.29638619465413285
1111	17	0.4380576111324004
1111	18	0.1708127513237585
1111	19	0.1732665300454599
1111	20	0.2870968690899929
1111	21	0.34849285740870073
1111	22	0.12428930952597739
1111	23	0.2985790684548988
1111	24	0.39170097642783475
1111	25	0.28228980974508744
1112	1	0.2637047425512833
1112	2	-0.1510388950004753
1112	3	0.2857897203389719
1112	4	0.3860444971503867
1112	5	0.36189096187969483
1112	6	0.32865181332167026
1112	7	0.46874923616484665
1112	8	0.3731598132917284
1112	9	0.4836835588124444
1112	10	0.39545769517735607
1112	11	0.4257393697402563
1112	12	0.6441110185958578
1112	13	0.5575240824689089
1112	14	0.3601235477884188
1112	15	0.011347043751676722
1112	16	0.001026849337755209
1112	17	0.25082296745760146
1112	18	0.4515679756405788
1112	19	0.4857655314580924
1112	20	0.4438727471162025
1112	21	0.568390368247023
1112	22	0.6502253025115081
1112	23	0.7352955183550378
1112	24	0.7161325610154918
1112	25	0.6641373425036221
1113	1	-0.6420377670024529
1113	2	-0.6126859440872998
1113	3	-0.5644374651723751
1113	4	-1.8747525240433163
1113	5	-0.6194715890316047
1113	6	-0.43598192787462153
1113	7	-0.5430712812301977
1113	8	-0.37693807616091846
1113	9	-0.17102042465389644
1113	10	-0.3392246062144082
1113	11	-0.536547327873663
1113	12	-0.1931088741024638
1113	13	-0.36155305910442337
1113	14	-0.4602704850735506
1113	15	-0.5026206002219453
1113	16	-0.7557515316741501
1113	17	-0.7834641064890491
1113	18	-0.6327895045385148
1113	19	-0.4789214584773518
1113	20	-0.13032511567303828
1113	21	-0.22470314536165858
1113	22	-0.16263856224605888
1113	23	-0.15572824330922685
1113	24	-0.1644434779107442
1113	25	-0.3632445723663196
1114	1	-0.7769293502814705
1114	2	-2.1102016143632305
1114	3	-1.319296421293945
1114	4	-0.9086708450996852
1114	5	-0.8337285048992058
1114	6	-0.6134806671668898
1114	7	-0.547583309645745
1114	8	-0.6801676012107762
1114	9	-0.9713373729994785
1114	10	-1.1569285477026554
1114	11	-1.14095692508117
1114	12	-1.0547689392324742
1114	13	-1.058305716328249
1114	14	-1.1298034576048366
1114	15	-1.3390486930581034
1114	16	-1.3709282151488722
1114	17	-1.5627934306958338
1114	18	-1.1963297955972862
1114	19	-1.8960955505088037
1114	20	-1.099071994981152
1114	21	-1.3199832296602239
1114	22	-1.028274673722045
1114	23	-0.8704293592375691
1114	24	-0.9611544085057275
1114	25	-1.613346817109779
1115	1	-1.2562670369923694
1115	2	-1.1900644182547673
1115	3	-0.8136459673120626
1115	4	-0.5474276157933744
1115	5	-0.9318617297576282
1115	6	-0.9003343503318106
1115	7	-0.6801221875262087
1115	8	-0.9162696128245784
1115	9	-1.7984538407441164
1115	10	-1.4776617394844465
1115	11	-1.7736896402307778
1115	12	-2.6183088371468983
1115	13	-2.729514153539661
1115	14	-2.23287146911386
1115	15	-2.323738938636673
1115	16	-2.10499128878957
1115	17	-2.0128180327233736
1115	18	-2.1890812884113373
1115	19	-2.8189836539133353
1115	20	-2.9488913575472395
1115	21	-2.4674041256670947
1115	22	-2.3622890569199413
1115	23	-2.386783698285356
1115	24	-2.703386587957745
1115	25	-2.86406191946757
1116	1	-1.7122814753251951
1116	2	-1.7813515493188525
1116	3	-1.8009172391657238
1116	4	-1.5038511995910184
1116	5	-1.8942019428694792
1116	6	-2.7272279220480335
1116	7	-2.1992720752709403
1116	8	-2.4613281547627324
1116	9	-3.4241509464481084
1116	10	-2.359998825874005
1116	11	-3.4791053013494917
1116	12	-5.521539156854253
1116	13	-4.341654543370687
1116	14	-3.885189250961652
1116	15	-5.029231382556038
1116	16	-4.297957746777211
1116	17	-4.23038902465623
1116	18	-4.088591402269547
1116	19	-4.4445584612389695
1116	20	-6.42469893747408
1116	21	-5.087361717022297
1116	22	-4.203967077744306
1116	23	-5.014745698714783
1116	24	-4.8780108618111235
1116	25	-4.161181080239473
1201	1	0.7729397112069805
1201	2	0.9075750010262631
1201	3	1.001661552708562
1201	4	0.8017063008370329
1201	5	0.325788001957946
1201	6	1.0065798147638176
1201	7	1.1016022448870615
1201	8	1.00630978486479
1201	9	0.9961740253875888
1201	10	1.0576502922262847
1201	11	0.8673538492392581
1201	12	1.1843756184693532
1201	13	1.147487506117777
1201	14	1.165104715607626
1201	15	1.0487204158693828
1201	16	1.0197730559680025
1201	17	0.8259083816109083
1201	18	1.1708516807641516
1201	19	1.12937683012656
1201	20	1.2616337709553633
1201	21	0.990870797392482
1201	22	1.046576414148845
1201	23	1.161112726200237
1201	24	0.827152720142351
1201	25	0.9520351463225438
1202	1	1.0997405315979805
1202	2	1.172471059512496
1202	3	1.1491102610070811
1202	4	1.1210996626676746
1202	5	1.187091281910618
1202	6	1.1730179044773976
1202	7	1.2248289319944448
1202	8	1.0035664010511667
1202	9	1.599433261116495
1202	10	1.4251764818968198
1202	11	1.3916209873315069
1202	12	1.4386763410303303
1202	13	1.281428766674786
1202	14	1.5860184709681349
1202	15	1.4794777122621028
1202	16	1.486336368101
1202	17	1.5022560763904735
1202	18	1.6773015561172002
1202	19	1.652129559583983
1202	20	1.5732783050197767
1202	21	1.7224098131534689
1202	22	1.61626660375493
1202	23	1.7778962134144294
1202	24	1.6242387668199285
1202	25	1.780119726700832
1203	1	0.7256055687430099
1203	2	0.744706120076728
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2211	9	-0.2572703629572075
2211	10	-0.326608295199442
2211	11	-0.06845604205745559
2211	12	-0.22153646706663466
2211	13	-0.0020234795679030526
2211	14	-0.035521891452233945
2211	15	-0.08871695964791826
2211	16	-0.006040435365711971
2211	17	-0.20910582348865603
2211	18	-0.16836208452904344
2211	19	-0.050800452191019474
2211	20	-0.17665657927362588
2211	21	-0.1513560762707442
2211	22	3.222916606517856E-4
2211	23	-0.13729735033677315
2211	24	-0.010924971554047147
2211	25	-0.177548129889459
2212	1	0.022209784199681337
2212	2	0.02860433327822065
2212	3	0.08715425343849148
2212	4	-0.016068773507385004
2212	5	-0.3444843689531112
2212	6	-0.15225365374118383
2212	7	-0.19969540312591505
2212	8	0.05616109602996744
2212	9	0.03440733168659449
2212	10	-0.3656093725479721
2212	11	-0.23518399882778596
2212	12	0.07764406940203372
2212	13	0.0714669600242317
2212	14	0.12218433172009213
2212	15	-0.1044324632871465
2212	16	-0.2380026942347513
2212	17	-0.12333127775648478
2212	18	-0.1079094708984627
2212	19	0.21571097448990173
2212	20	0.011207980201106693
2212	21	0.05499285740869908
2212	22	0.11484486508152969
2212	23	-0.1930505611747293
2212	24	-0.2618916161647604
2212	25	-0.19413611618083593
2213	1	-0.6528585840774355
2213	2	-0.16796501410015097
2213	3	-0.30164474382677753
2213	4	-0.6410848105106552
2213	5	-0.3905143665897697
2213	6	-0.4292067919295093
2213	7	-0.9568107682631535
2213	8	-0.7679214185721435
2213	9	-0.6448939614023033
2213	10	-0.7162546509165892
2213	11	-0.45101209903373274
2213	12	-0.8492271956643052
2213	13	-0.5007974648381861
2213	14	-0.7678571112725834
2213	15	-0.8362021888011739
2213	16	-1.3519358958958492
2213	17	-1.0690202780603428
2213	18	-0.7455360788190504
2213	19	-0.702940904371637
2213	20	-0.6907595068848877
2213	21	-0.6235103837540663
2213	22	-0.7057745008659992
2213	23	-0.8906821490068708
2213	24	-0.6732061440016253
2213	25	-0.6334727750822182
2214	1	-0.9268534476504335
2214	2	-0.3405397815626152
2214	3	-0.4466572219713463
2214	4	-0.4402989756064386
2214	5	-0.883749483013311
2214	6	-1.332514300805105
2214	7	-0.9886363346897937
2214	8	-0.7692704244835156
2214	9	-0.5924878890610898
2214	10	-1.0022550053001638
2214	11	-1.0196698605677454
2214	12	-0.8265453659349404
2214	13	-1.0958328925215213
2214	14	-0.7359397466908746
2214	15	-0.795373785171094
2214	16	-0.8087254368837264
2214	17	-0.9058351809571121
2214	18	-0.899494820885451
2214	19	-1.0079554319633885
2214	20	-1.2912315201892142
2214	21	-1.4650663411341043
2214	22	-1.7876076459775376
2214	23	-1.7187553057319391
2214	24	-1.605084867964571
2214	25	-1.9178361335283967
2215	1	-1.4570474621771297
2215	2	-0.8724121723578477
2215	3	-0.9458167610542638
2215	4	-1.0059942968568194
2215	5	-1.2136168166181704
2215	6	-1.216092220568875
2215	7	-1.6663318765558657
2215	8	-1.3687080666109317
2215	9	-1.4285958889253223
2215	10	-1.5280288572500709
2215	11	-1.127011696090264
2215	12	-1.4253752542694613
2215	13	-1.5835350528582097
2215	14	-1.8561570531430363
2215	15	-1.5571196613547826
2215	16	-1.4545485879545623
2215	17	-1.6430776545680814
2215	18	-1.361955364618268
2215	19	-1.6412938564279713
2215	20	-1.7095347734220852
2215	21	-1.4314805725429949
2215	22	-1.61308387887982
2215	23	-1.9067784194678055
2215	24	-1.7996734197074318
2215	25	-2.121078547742833
2216	1	-1.7436813483727498
2216	2	-0.9965497167331598
2216	3	-1.0351789335676571
2216	4	-1.362472376407915
2216	5	-2.191858202400258
2216	6	-2.4992937799077346
2216	7	-2.1428229028820347
2216	8	-2.3483522847784073
2216	9	-4.243437919183243
2216	10	-2.168135548855979
2216	11	-2.3854469813010404
2216	12	-2.8166589254277383
2216	13	-2.537719135641229
2216	14	-2.828786711755342
2216	15	-2.7482806244421596
2216	16	-2.032538593171445
2216	17	-2.244223141964852
2216	18	-2.8545451649770897
2216	19	-2.9980681690082918
2216	20	-2.413206711048531
2216	21	-2.8342167318996587
2216	22	-2.3506536228708654
2216	23	-3.5264342960949016
2216	24	-2.7396848395901188
2216	25	-2.8748253985272747
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41897.0
3	1.0
4	685.0
5	6132.0
6	7891.0
7	6220.0
8	4897.0
9	3931.0
10	3301.0
11	2982.0
12	2691.0
13	2486.0
14	2441.0
15	2340.0
16	2445.0
17	2554.0
18	2897.0
19	3574.0
20	4737.0
21	6279.0
22	7776.0
23	9316.0
24	10884.0
25	12470.0
26	13750.0
27	15044.0
28	16805.0
29	20363.0
30	25195.0
31	31941.0
32	40434.0
33	51548.0
34	67551.0
35	88599.0
36	121211.0
37	187141.0
38	573084.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60414871949932	18.737484525025245	13.4045372340729	26.253829521402533
2	14.240245784489488	20.38214925990213	38.92070099903411	26.456903956574273
3	17.605130438066947	25.254038352860857	29.255654620437234	27.88517658863496
4	11.993390446073636	16.76194561440136	35.83787639222102	35.406787547303985
5	13.501327620928949	38.37722392539668	33.5496151110769	14.571833342597461
6	32.55365960425997	35.4962577657243	17.728023909233887	14.222058720781842
7	28.684514124285727	31.68983755894923	20.89537233382627	18.73027598293877
8	26.75435970254796	34.72179452086042	20.09839982663838	18.425445949953236
9	27.722469584848863	14.134066109483143	19.25012542330365	38.89333888236434
10	16.65143591966149	28.245646049795276	31.897817607408847	23.205100423134386
11	35.064281749512446	22.403880205011863	22.99550173824007	19.536336307235626
12	24.301188963805252	24.726485599123897	29.762855622159318	21.20946981491153
13	29.734311237850118	21.2319430757534	25.258281798107323	23.77546388828916
14	22.08245902164968	21.892218299447446	26.915499631275004	29.10982304762787
15	24.80706330033991	29.002715009513224	23.06945721187763	23.120764478269233
16	23.188686693639756	27.1534174069065	26.117414174696123	23.54048172475762
17	22.351896675927318	27.624055082230736	26.537359567174402	23.486688674667548
18	22.452111866167606	25.8698884943784	28.85522367746951	22.822775961984483
19	23.880986242650348	26.12499893121895	27.435181992036867	22.55883283409383
20	24.895492143474485	25.161687031478113	27.47699829638122	22.465822528666184
21	24.717259010198127	25.164500286071966	26.861266853486264	23.256973850243643
22	24.51047032754063	26.355513754996473	26.573897925884758	22.56011799157814
23	23.460394886187533	26.140137499795152	27.297179252051844	23.102288361965467
24	23.978388207137478	25.54063326286629	27.418163111607967	23.062815418388265
25	23.336418493002814	26.041241388450103	27.548837080056686	23.073503038490394
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	181.0
1	181.0
2	409.5
3	638.0
4	638.0
5	638.0
6	1386.5
7	2135.0
8	2135.0
9	2135.0
10	2260.5
11	2386.0
12	2386.0
13	2386.0
14	3051.0
15	3716.0
16	3716.0
17	3716.0
18	7475.0
19	11234.0
20	11234.0
21	11234.0
22	18983.0
23	26732.0
24	26732.0
25	26732.0
26	41565.5
27	56399.0
28	56399.0
29	56399.0
30	70744.5
31	85090.0
32	85090.0
33	85090.0
34	97239.0
35	109388.0
36	109388.0
37	109388.0
38	120065.5
39	130743.0
40	130743.0
41	130743.0
42	143903.5
43	157064.0
44	157064.0
45	157064.0
46	177380.5
47	197697.0
48	197697.0
49	197697.0
50	195026.5
51	192356.0
52	192356.0
53	192356.0
54	173150.5
55	153945.0
56	153945.0
57	153945.0
58	134683.0
59	115421.0
60	115421.0
61	115421.0
62	98088.0
63	80755.0
64	80755.0
65	80755.0
66	61549.5
67	42344.0
68	42344.0
69	42344.0
70	31841.5
71	21339.0
72	21339.0
73	21339.0
74	15157.5
75	8976.0
76	8976.0
77	8976.0
78	6235.0
79	3494.0
80	3494.0
81	3494.0
82	2269.0
83	1044.0
84	1044.0
85	1044.0
86	684.0
87	324.0
88	324.0
89	324.0
90	201.5
91	79.0
92	79.0
93	79.0
94	45.5
95	12.0
96	12.0
97	12.0
98	6.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0875672340367925
2	0.04545801083439675
3	0.01852520817702689
4	0.004987556047661086
5	0.016458934957281582
6	0.06091943458214612
7	0.07231956269108573
8	0.04595676643916286
9	0.0174564461668138
10	0.044816753628268896
11	0.004417549642214104
12	1.425016013617453E-4
13	0.005985067257193302
14	5.700064054469812E-4
15	0.013038896524599695
16	0.008478845281023845
17	0.0045600512435758495
18	0.004702552844937595
19	0.0017812700170218162
20	7.125080068087266E-4
21	0.0
22	2.1375240204261797E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1403493.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.502106946400595
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.68714100952033	36.70853768890896
2	14.705217648077722	14.852889486666374
3	5.368027926413118	8.132901612929407
4	2.505370926861476	5.061060419550442
5	1.4277734283087105	3.605278318583776
6	0.8305850259187437	2.5167776284216385
7	0.553392592178887	1.9563244311494803
8	0.3703566863341607	1.496303438524986
9	0.2547931944284005	1.158083383881431
>10	1.1645411606172351	9.978146828025313
>50	0.06455862548544668	2.2805268004684054
>100	0.057198561446757036	5.631417030980028
>500	0.006512664907829972	2.116492733449162
>1k	0.004247390157280417	3.5431465461754597
>5k	2.831593438186945E-4	0.962113652285123
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7718	0.5499136796549752	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5738	0.40883709430684734	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4625	0.329534953149036	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3496	0.24909279918033078	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2618	0.1865345961825246	No Hit
GGATACCACGTGTCCCGCCCTACTC	2264	0.16131181274149567	No Hit
ATCATTAACTGAATCCATAGGTTAA	2046	0.14577913819306543	No Hit
GTCCTACAGTGGACATTTCTAAATT	1809	0.12889269843169862	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1779	0.12675517441127246	No Hit
CCCATGTACTCTGCGTTGATACCAC	1739	0.12390514238403755	No Hit
GTACATGGGAAGCAGTGGTATCAAC	1736	0.12369138998199493	No Hit
TATTCAGACAGGATACCACGTGTCC	1648	0.11742131952207813	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1600	0.11400128108939624	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1591	0.1133600238832684	No Hit
CCCATATTCAGACAGGATACCACGT	1591	0.1133600238832684	No Hit
CTATCGGTCAGTCAGGAGTATTTAG	1504	0.10716120422403247	No Hit
GTATTTAGCCTTGGAGGATGGTCCC	1493	0.10637744541654287	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1445	0.10295740698386098	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	7.125080068087265E-5
6	0.0	0.0	0.0	0.0	7.125080068087265E-5
7	0.0	0.0	0.0	0.0	7.125080068087265E-5
8	0.0	0.0	0.0	0.0	7.125080068087265E-5
9	0.0	0.0	0.0	0.0	7.125080068087265E-5
10	0.0	0.0	0.0	0.0	1.425016013617453E-4
11	0.0	0.0	0.0	0.0	1.425016013617453E-4
12	0.0	0.0	0.0	0.0	2.1375240204261794E-4
13	0.0	0.0	0.0	7.125080068087265E-5	2.1375240204261794E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCAC	50	0.0014996531	13.299726	8
ACTTATT	80	2.8664985E-5	11.874755	8
AGGACGA	105	2.6908674E-7	11.771315	5
GTAAGAC	65	7.966534E-4	11.70208	3
TATTCTA	65	7.968919E-4	11.701662	5
GGACCGT	65	7.968919E-4	11.701662	6
TCGAACT	205	0.0	11.577285	19
GCCTCGA	225	0.0	11.393671	16
CTCGAAC	225	0.0	11.392859	18
CCGTACA	280	0.0	11.189416	17
GGTATCA	1710	0.0	11.121587	1
GTAATAC	60	0.0058387546	11.092595	3
CCTAGAC	95	1.3472883E-5	11.009192	3
TACTAAG	70	0.001480888	10.866216	2
CTAGACA	105	3.4317782E-6	10.8658285	4
GGCGTGT	70	0.001491502	10.856919	8
CCCGTAC	280	0.0	10.851115	16
GGCGAGG	485	0.0	10.76568	19
TAGAAAT	250	0.0	10.648512	4
TTAGGGT	90	9.43582E-5	10.563999	4
>>END_MODULE