FastQCFastQC Report
Thu 26 May 2016
SRR1512884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018924
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT60980.3020420778592953No Hit
GTCCTACAGTGGACATTTCTAAATT57270.2836659527550319No Hit
GTCCTAAAGTGTGTATTTCTCATTT49110.24324838379255484No Hit
CTGTAGGACGTGGAATATGGCAAGA46710.23136086350947338No Hit
CTTTAGGACGTGAAATATGGCGAGG38590.19114141988504768No Hit
TATCAACGCAGAGTACTTTTTTTTT36210.17935296227099187No Hit
GGTATCAACGCAGAGTACTTTTTTT33370.16528606326934547No Hit
GTACTGGTTCACTATCGGTCAGTCA31800.15750964375082965No Hit
GTCCTACAGTGTGCATTTCTCATTT27240.13492335521297485No Hit
CTGAAGGACCTGGAATATGGCGAGA21460.1062942438645536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17850.012.9108341
TAGGACC6150.012.5099424
ACCGACC550.003071991512.0893318
GGTCTAT1209.664291E-911.9038421
TTAGACT1603.6379788E-1211.8728634
TAAGTAG1201.00462785E-811.8728625
GTACTAG1156.842856E-811.5933081
CCATCGC1104.978774E-711.22580719
TTAGGAC15100.011.0079523
GCGCCAC2100.010.85599613
AGACCGT700.001493361110.8557256
GTATCAA38100.010.6728421
GGCGAGG11100.010.61122819
CACCTTT6400.010.53794914
AATGTCC6100.010.4330658
GTCCTAA13350.010.4147481
GGACCTG15050.010.4139236
AGGACCT15350.010.3955235
GTATTAG1658.913048E-1010.3888081
TAGAAAT6150.010.347734
Produced by FastQC (version 0.11.5)