##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512884_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2018924
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.964376073591676	31.0	30.0	33.0	23.0	34.0
2	29.1081551361022	31.0	30.0	34.0	23.0	34.0
3	29.2147648945676	31.0	30.0	34.0	23.0	34.0
4	32.68529127396574	35.0	33.0	37.0	25.0	37.0
5	32.860686187295805	35.0	33.0	37.0	26.0	37.0
6	32.85021823505986	36.0	33.0	37.0	26.0	37.0
7	32.847304801963816	37.0	35.0	37.0	26.0	37.0
8	32.77094135291868	37.0	33.0	37.0	25.0	37.0
9	34.09884968428727	39.0	34.0	39.0	25.0	39.0
10	33.94739821806071	38.0	34.0	39.0	23.0	39.0
11	34.027207066734555	39.0	34.0	39.0	25.0	39.0
12	33.925520722919735	38.0	34.0	39.0	23.0	39.0
13	33.904058300361974	38.0	34.0	39.0	23.0	39.0
14	34.760658152560474	39.0	34.0	41.0	21.0	41.0
15	34.94176799126664	39.0	34.0	41.0	24.0	41.0
16	34.89147536014233	39.0	34.0	41.0	23.0	41.0
17	34.66091294174521	39.0	34.0	41.0	19.0	41.0
18	34.6648784203863	39.0	34.0	41.0	19.0	41.0
19	34.778427518816954	39.0	34.0	41.0	22.0	41.0
20	34.751491883795524	39.0	34.0	41.0	19.0	41.0
21	34.64782428660019	39.0	34.0	41.0	19.0	41.0
22	34.66408740497413	39.0	34.0	41.0	19.0	41.0
23	34.32723074271245	39.0	34.0	41.0	17.0	41.0
24	34.398886238412146	39.0	34.0	41.0	17.0	41.0
25	34.10934636469724	39.0	34.0	41.0	15.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9964885738209226
1101	2	0.8191222282632538
1101	3	1.0518108614740633
1101	4	1.2224838990887221
1101	5	1.101923991675669
1101	6	1.112963256469044
1101	7	0.854658436521504
1101	8	1.146754714358437
1101	9	1.277768687575957
1101	10	1.2738732623685465
1101	11	1.3169593185713069
1101	12	1.1707959089964888
1101	13	1.4680577345687595
1101	14	1.7177793102242802
1101	15	1.5049895952692083
1101	16	1.47858339006941
1101	17	0.823964128846356
1101	18	1.2964422648927183
1101	19	0.7478842097871237
1101	20	0.09356021800884662
1101	21	1.0947409027582253
1101	22	1.1726480619367123
1101	23	0.8289256122384643
1101	24	1.3745880316845032
1101	25	1.0304292741772372
1102	1	0.9334663545676989
1102	2	0.9997108006885611
1102	3	0.9308873116317145
1102	4	1.0189757075648984
1102	5	1.0227828597338444
1102	6	0.8950676093940686
1102	7	0.8838732897919357
1102	8	0.9361843323594812
1102	9	1.3096745151396192
1102	10	1.3315776846409193
1102	11	1.100563334567859
1102	12	1.2064389652213947
1102	13	1.104996971334316
1102	14	1.3945712209718977
1102	15	1.4067106078890461
1102	16	1.3736070810983136
1102	17	1.39515348370918
1102	18	1.5682814596151076
1102	19	1.2902174301924205
1102	20	1.5549686797646842
1102	21	1.6407877058610438
1102	22	1.291956475817429
1102	23	1.3975229241271308
1102	24	0.8639436897765904
1102	25	1.1829582524118223
1103	1	0.6429121284285628
1103	2	0.7670873232254891
1103	3	0.8609269656066978
1103	4	0.9425631369985155
1103	5	0.8098958891573318
1103	6	0.541950830429542
1103	7	0.4556786064076661
1103	8	0.6490303859382109
1103	9	0.7099400278543087
1103	10	0.8758382959624811
1103	11	0.6501206126518184
1103	12	0.748675343180814
1103	13	0.8275398901769151
1103	14	0.9156623120908094
1103	15	0.8922669076707521
1103	16	0.8476482568768091
1103	17	0.4594509172406802
1103	18	0.9708883510257209
1103	19	0.8679271467904854
1103	20	1.049886171024653
1103	21	0.8208240401550739
1103	22	0.9922785311909408
1103	23	0.2507550427042844
1103	24	0.48329239616640507
1103	25	0.8185493084810744
1104	1	0.9921686810422159
1104	2	0.8954061093045098
1104	3	0.9276580918934343
1104	4	1.0973399969624111
1104	5	0.8517232891519555
1104	6	0.7232045095711968
1104	7	1.0031306524714978
1104	8	0.6085469025064754
1104	9	1.1235340537899319
1104	10	1.0841837608260931
1104	11	0.929140064521313
1104	12	1.1336383529734135
1104	13	1.1178821036392392
1104	14	1.3666172184747012
1104	15	1.0127208340083982
1104	16	0.9784241880416715
1104	17	1.1572145130957878
1104	18	1.1142390479424478
1104	19	0.7400930172357221
1104	20	0.9262201480471575
1104	21	0.9825992435792941
1104	22	0.8327128535803467
1104	23	1.1240163034098885
1104	24	1.0226494991358095
1104	25	0.6803656608466255
1105	1	0.5388717482773444
1105	2	0.589830781796234
1105	3	0.7735536766146396
1105	4	0.9250676790681496
1105	5	0.6365686321307749
1105	6	0.8451485323484889
1105	7	0.8583318663886956
1105	8	0.8444254914185265
1105	9	0.6035370437933949
1105	10	0.7640518375154457
1105	11	0.7986975319036418
1105	12	0.8867497118391938
1105	13	0.8575257363844955
1105	14	0.9955190519399792
1105	15	0.933807742635345
1105	16	0.8843313817236833
1105	17	1.2989788088228664
1105	18	1.0469085884019194
1105	19	0.810366874921236
1105	20	1.0757956765030343
1105	21	0.8909453479800504
1105	22	0.8628431910996852
1105	23	1.1330081032951327
1105	24	1.136187920075038
1105	25	1.3337676674173693
1106	1	0.44111048403084396
1106	2	0.5504850776325334
1106	3	0.4322067124589566
1106	4	0.4945233052596194
1106	5	0.37143788750823603
1106	6	0.47393178100054456
1106	7	0.4716323499310704
1106	8	0.5742095746107267
1106	9	0.8374163054708177
1106	10	0.5129342082639994
1106	11	0.36658962259033956
1106	12	0.6480231628263269
1106	13	0.5532841042857939
1106	14	0.8293359276507744
1106	15	0.5591867629506737
1106	16	0.5758663219558855
1106	17	0.8295045691134888
1106	18	0.7627728283567521
1106	19	0.8000952811950199
1106	20	0.8458133900266773
1106	21	0.7780664299367999
1106	22	0.675991902880611
1106	23	1.0141777685790743
1106	24	0.6743996314550529
1106	25	0.8475226480674962
1107	1	0.7547284794012512
1107	2	0.7700672292082551
1107	3	0.7230764992290624
1107	4	0.7728890431108866
1107	5	0.7110586321932928
1107	6	0.8513652799774505
1107	7	0.5864462576710281
1107	8	0.8228509154546373
1107	9	0.6811600345843232
1107	10	0.8400860604962332
1107	11	0.7369637821927952
1107	12	0.7377689302248314
1107	13	0.6442854315411424
1107	14	0.8974819968418615
1107	15	0.5616078189087048
1107	16	0.6969357105493899
1107	17	1.0527299576121862
1107	18	1.0203527994415467
1107	19	0.7859937856626544
1107	20	0.9868145197737874
1107	21	0.7304993363889949
1107	22	0.8031889844989166
1107	23	1.1827170766682258
1107	24	0.9098193759583992
1107	25	1.0619695855047837
1108	1	0.7590206503600889
1108	2	0.7569427410837655
1108	3	0.7660967922493569
1108	4	0.825642554197735
1108	5	0.6843303887983794
1108	6	0.700635762581264
1108	7	0.6565436277683929
1108	8	0.7876141635512184
1108	9	0.8869339903582798
1108	10	0.9363800817902543
1108	11	0.6853363972320778
1108	12	0.7995147669706668
1108	13	0.8928051633942076
1108	14	0.9193174686773276
1108	15	0.9651879141554716
1108	16	0.6981778617915424
1108	17	1.0243439958928917
1108	18	1.034086988175737
1108	19	1.0244253990942696
1108	20	1.1013540593133229
1108	21	1.0448045506942094
1108	22	1.1077988974421586
1108	23	1.2884364323875772
1108	24	1.2092771670828597
1108	25	1.3237214889233542
1109	1	0.8866071368981494
1109	2	0.7768680373228172
1109	3	0.9055166216614587
1109	4	0.9436453973293766
1109	5	0.7167273014245552
1109	6	0.9074001261204856
1109	7	0.8001523059984024
1109	8	0.7848001310746398
1109	9	0.9144455823211857
1109	10	0.9739509215635707
1109	11	0.8503191285465945
1109	12	0.8133521360244202
1109	13	0.951574507184155
1109	14	1.0907139424308383
1109	15	0.8927629518412772
1109	16	0.645052325126592
1109	17	1.0699753046875173
1109	18	1.0574755622211853
1109	19	1.01087568245336
1109	20	0.8359310242225959
1109	21	0.889489421035961
1109	22	0.8997627572318834
1109	23	1.2056526439623667
1109	24	1.0028276469805846
1109	25	0.4755824044499093
1110	1	0.7943972956316117
1110	2	0.7342289219370031
1110	3	0.6530776953815156
1110	4	0.7346309720910256
1110	5	0.48788799314696263
1110	6	0.5677545245357081
1110	7	0.5036394069523524
1110	8	0.61153873621533
1110	9	0.7252683443080628
1110	10	0.6385795102450444
1110	11	0.6818644112636605
1110	12	0.6437976138101575
1110	13	0.6259901293358539
1110	14	0.8348007872684278
1110	15	0.8046039325416885
1110	16	0.8961808555998019
1110	17	0.9144818169145665
1110	18	0.28238992981915345
1110	19	0.760596046165098
1110	20	0.9146872792448306
1110	21	1.0017889692830408
1110	22	0.9755833281271862
1110	23	1.1651629752194523
1110	24	0.9406592329617567
1110	25	1.1743631542669988
1111	1	0.41914940687035696
1111	2	0.38565902685687803
1111	3	0.49309162216265534
1111	4	0.45383763727751614
1111	5	0.49536238538215827
1111	6	0.4563964439956436
1111	7	0.40145704025438533
1111	8	0.6060695810852863
1111	9	0.6302940891847157
1111	10	0.537311726838638
1111	11	0.20617878584263138
1111	12	-0.029235833537192946
1111	13	0.34019476526702874
1111	14	0.24309357647135243
1111	15	0.326176826683394
1111	16	0.42092509945539547
1111	17	0.004099064941762265
1111	18	0.3026060786065017
1111	19	0.45452628441442045
1111	20	0.7096748105772548
1111	21	0.4816242610778474
1111	22	0.317064257098707
1111	23	0.5587507553578632
1111	24	0.5157176615200569
1111	25	0.5639648566476794
1112	1	0.24046983536553768
1112	2	0.21984083025574108
1112	3	0.19974438433644082
1112	4	0.28993528945649416
1112	5	0.2824602824165936
1112	6	-0.05667784462618641
1112	7	-0.09727634324902823
1112	8	0.058763300252373085
1112	9	-0.014491548009935684
1112	10	0.09538068840232938
1112	11	0.051002715658476916
1112	12	0.12377672228357284
1112	13	0.16094146013984556
1112	14	0.23853780366199828
1112	15	-0.05593426200916696
1112	16	0.14440675674654813
1112	17	0.46254932284556105
1112	18	0.12363893775953017
1112	19	0.14043995778398255
1112	20	-0.08598712627626526
1112	21	0.0813428178057265
1112	22	-0.10258484086089936
1112	23	0.07114293324694643
1112	24	0.1213948048905138
1112	25	0.06837459668686563
1113	1	-0.2180965759004394
1113	2	-0.04998872309068858
1113	3	-0.03759825794102056
1113	4	-0.07889477128974676
1113	5	-0.10014809279848436
1113	6	-0.4622002821862452
1113	7	-0.48848925558224465
1113	8	-0.26024264964827637
1113	9	-0.41400058908097037
1113	10	-0.25793455995429326
1113	11	-0.3366839096791949
1113	12	-0.4940729796544687
1113	13	-0.31476089175752264
1113	14	-0.05831319937389878
1113	15	-0.2814689451960959
1113	16	-0.35171829604854565
1113	17	-0.16858319921498577
1113	18	-0.36436403293883757
1113	19	-0.6846088688185077
1113	20	-0.4369204301293905
1113	21	-0.12736575044804965
1113	22	-0.7993604425832217
1113	23	-4.592508531267665
1113	24	-2.083303887491965
1113	25	-1.2084840031731048
1114	1	-0.8650466900727132
1114	2	-1.0956487057847895
1114	3	-0.824335507520292
1114	4	-0.7668078279112827
1114	5	-0.8211155189567663
1114	6	-1.4522669393174183
1114	7	-1.2757247852229092
1114	8	-1.1831102757475165
1114	9	-1.6288589109767884
1114	10	-1.1748187676616055
1114	11	-1.2483312061142442
1114	12	-2.1017488460399356
1114	13	-1.3262712423287866
1114	14	-3.340466110082154
1114	15	-2.107740335379205
1114	16	-2.0826445268621754
1114	17	-1.87202064183721
1114	18	-1.6465457529991738
1114	19	-1.9134341471990126
1114	20	-1.3629086974193072
1114	21	-1.3410053555976233
1114	22	-1.5954559638448274
1114	23	-6.227039887305608
1114	24	-3.342012274969637
1114	25	-3.2830098407999415
1115	1	-1.5830306671265504
1115	2	-1.5262040250853879
1115	3	-1.2167666682260432
1115	4	-1.357147200731326
1115	5	-1.1236735728747362
1115	6	-1.356006949782838
1115	7	-1.9863806397827375
1115	8	-2.3691321972374304
1115	9	-3.5210262086466955
1115	10	-4.349751759005763
1115	11	-2.917010663759264
1115	12	-3.2514595282200496
1115	13	-2.4987463383563018
1115	14	-3.184648811035217
1115	15	-2.948187339207344
1115	16	-3.015731756782259
1115	17	-2.868888913633551
1115	18	-5.560260717739133
1115	19	-3.6086591780201616
1115	20	-3.699357659868543
1115	21	-3.4414931275810794
1115	22	-2.8514004760606966
1115	23	-3.485091759983895
1115	24	-3.1862290978885817
1115	25	-3.5506888667059044
1116	1	-1.1948792197500921
1116	2	-1.0441165575112095
1116	3	-1.4705275270583336
1116	4	-1.419176706372717
1116	5	-1.262134211104101
1116	6	-3.6186887284104436
1116	7	-2.1666017545870773
1116	8	-2.4382385200871717
1116	9	-3.4754954819284762
1116	10	-2.876699351775404
1116	11	-2.312058635814026
1116	12	-2.1819387492737583
1116	13	-3.433781377234265
1116	14	-6.502047050504977
1116	15	-4.315722072609702
1116	16	-3.133715415488396
1116	17	-4.37385557551168
1116	18	-2.9860734892615426
1116	19	-2.736222902709031
1116	20	-3.4835167474376654
1116	21	-4.244135966324631
1116	22	-3.7691542643710534
1116	23	-2.861902838180697
1116	24	-3.799562716057281
1116	25	-3.796736260008231
1201	1	0.7167172001510806
1201	2	0.8063751201558311
1201	3	1.0806511047059395
1201	4	1.0221268387455282
1201	5	0.5437319341410216
1201	6	1.0378799827804102
1201	7	1.0871055816274833
1201	8	1.1408367757652726
1201	9	1.5894677185583461
1201	10	1.533147110418092
1201	11	1.6502950215358823
1201	12	1.3205112570441528
1201	13	1.4224454568630591
1201	14	1.4775264921133981
1201	15	1.4742866064640978
1201	16	1.5195609922072464
1201	17	1.790192898431492
1201	18	1.36781975845836
1201	19	1.667564589617463
1201	20	1.6153818827932724
1201	21	1.7558717877239332
1201	22	1.6191263321489942
1201	23	1.950962482623403
1201	24	1.8048873214952508
1201	25	1.7617789030337505
1202	1	0.6232146590628496
1202	2	0.7534404043812337
1202	3	0.7092989200373978
1202	4	0.9540338924983232
1202	5	0.8359504824274921
1202	6	1.0093224190236043
1202	7	1.0951034461456217
1202	8	1.057657375440428
1202	9	1.3632952554381603
1202	10	1.1092335759756722
1202	11	1.1192073130343019
1202	12	1.382243999300485
1202	13	1.1916351013639144
1202	14	1.6685769090763216
1202	15	1.4118137841293432
1202	16	1.0426857051418992
1202	17	1.424460730386322
1202	18	1.4728566267415957
1202	19	1.4604106180922045
1202	20	1.2893991389544297
1202	21	1.5250856795570158
1202	22	1.4861838958521574
1202	23	1.5217353491719905
1202	24	1.5550543974161144
1202	25	1.933615240407697
1203	1	1.0577367196873588
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2211	16	-0.018410432177326186
2211	17	0.45863007207273654
2211	18	-0.031119393261811013
2211	19	0.16223666338837717
2211	20	-0.003350380618748261
2211	21	0.16601149024036488
2211	22	0.20394339611417678
2211	23	0.3500925899879519
2211	24	0.002443701149253741
2211	25	0.37692299383748207
2212	1	-0.1531182007121572
2212	2	-0.1296972212159524
2212	3	-0.4644203648700618
2212	4	-0.13909924540897123
2212	5	-0.4246970253578439
2212	6	-0.5149272399437237
2212	7	-0.42691711855757575
2212	8	-0.3663219670500055
2212	9	-0.2770622396134428
2212	10	-0.6841744265352219
2212	11	-0.6927980205661797
2212	12	-0.17820803122810247
2212	13	-0.22910667146924624
2212	14	-0.18293494867876348
2212	15	-0.6280763601722654
2212	16	-0.5469420460522159
2212	17	-0.37394785549352605
2212	18	-0.12684696651410832
2212	19	-0.37158465431446785
2212	20	-0.2465366447743591
2212	21	0.0763890097998754
2212	22	-0.017354987199631466
2212	23	-0.0026673481129932952
2212	24	-0.021716188824100868
2212	25	0.09528250171119623
2213	1	-0.27862703736134264
2213	2	-0.21468599890780382
2213	3	-0.3355619144978448
2213	4	-0.18806294438977034
2213	5	-0.17640886921226695
2213	6	0.042550201250868724
2213	7	-0.2789412249730958
2213	8	-0.07678899856581012
2213	9	-0.43196613606396994
2213	10	-0.310764882390103
2213	11	-0.31133526389385935
2213	12	-0.3530733774316417
2213	13	-0.43412082496246285
2213	14	-0.5665525036970678
2213	15	-0.2783267024417455
2213	16	-0.30539005505071515
2213	17	-0.41592695352260023
2213	18	-0.4047047428726316
2213	19	-0.38065256129343084
2213	20	-0.2968069651751293
2213	21	-0.4071409472689851
2213	22	-0.6820724952315942
2213	23	-0.3387583983470748
2213	24	-0.12900848477211468
2213	25	-0.0285209452512305
2214	1	-2.809150796053899
2214	2	-1.4259042109266105
2214	3	-1.5777027465890932
2214	4	-3.284453761313628
2214	5	-1.7873021711286015
2214	6	-1.5305767079956638
2214	7	-1.2243541961255389
2214	8	-1.0063631146348797
2214	9	-0.9960102379166855
2214	10	-1.0137820453173987
2214	11	-0.9560264312254318
2214	12	-0.9269984193674219
2214	13	-1.326874665591717
2214	14	-1.1743307515273074
2214	15	-1.4787797564983265
2214	16	-1.3047620430938878
2214	17	-3.483159486837568
2214	18	-3.3876848809696725
2214	19	-2.236599244226099
2214	20	-1.9518488517689647
2214	21	-1.9205218652158607
2214	22	-3.071223055769156
2214	23	-1.7483123786802963
2214	24	-2.846547399377247
2214	25	-2.879318094109742
2215	1	-1.6386639998327013
2215	2	-3.1816826397335447
2215	3	-1.7963645423913022
2215	4	-3.439814348760482
2215	5	-2.0356274538010197
2215	6	-1.8617252853034714
2215	7	-2.0624943197284225
2215	8	-1.523821542035659
2215	9	-3.946977134759905
2215	10	-2.2083226180731756
2215	11	-1.7524712233941173
2215	12	-1.6220307385096007
2215	13	-1.7963873093600924
2215	14	-1.4344594803485933
2215	15	-1.6351744216018673
2215	16	-1.7178129635752697
2215	17	-1.9061723593109292
2215	18	-1.6803563473753798
2215	19	-1.7518641271164697
2215	20	-1.618981001559078
2215	21	-2.1333724286803744
2215	22	-2.133726044875935
2215	23	-2.07657915048177
2215	24	-1.9577509598990446
2215	25	-2.950965297348155
2216	1	-3.162989858771631
2216	2	-3.2681796954023064
2216	3	-4.574927082865429
2216	4	-5.114911981053773
2216	5	-5.105734400508833
2216	6	-4.01487285898796
2216	7	-5.652883981659215
2216	8	-3.448884663099122
2216	9	-4.271315167890879
2216	10	-3.3000830342183143
2216	11	-3.5090570320098387
2216	12	-3.8292960686583513
2216	13	-3.4145843886923153
2216	14	-4.502392050759461
2216	15	-3.8448544284626323
2216	16	-4.009489723148771
2216	17	-3.920416742807241
2216	18	-3.5131352340464836
2216	19	-4.196725503874816
2216	20	-4.812488915893287
2216	21	-4.397864564702175
2216	22	-4.029518723208792
2216	23	-4.152105349063291
2216	24	-4.666429393472189
2216	25	-4.598304324769124
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	89077.0
3	1.0
4	781.0
5	4852.0
6	5942.0
7	5547.0
8	5003.0
9	4520.0
10	4112.0
11	3819.0
12	3615.0
13	3648.0
14	3559.0
15	3617.0
16	3875.0
17	4124.0
18	4694.0
19	5712.0
20	7314.0
21	9775.0
22	11992.0
23	14768.0
24	17083.0
25	19326.0
26	21901.0
27	24242.0
28	27287.0
29	32371.0
30	39317.0
31	49067.0
32	61291.0
33	78468.0
34	101407.0
35	133650.0
36	183389.0
37	287528.0
38	742250.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.893737238008015	18.254922296271523	12.485619853274052	24.365720612446413
2	15.187774682270138	19.70683054803695	41.816796585518574	23.28859818417434
3	19.615369754467935	25.41791374525813	28.518445865148077	26.44827063512586
4	12.455825049859364	16.19698634452845	37.56859687379562	33.77859173181656
5	13.347521018977021	38.82239236876886	34.30056294992897	13.529523662325154
6	32.17250007931221	35.54262436153675	18.563616319279213	13.72125923987183
7	29.046022433234658	31.384222183007132	21.33878665426765	18.230968729490563
8	26.105295412321833	36.046099405065	20.337217425709976	17.511387756903186
9	28.142516015527985	14.516069065926521	19.6865452710383	37.65486964750719
10	16.805883236553484	28.12572969098036	32.8959442690845	22.17244280338165
11	34.85404915560588	22.11357802403943	24.386100240632246	18.646272579722446
12	24.86555468021424	24.25667739717232	30.596765181401402	20.281002741212035
13	29.827315697243545	21.784297055666954	25.4174835730556	22.970903674033902
14	21.821510528906714	22.324147651079908	26.947923343748965	28.906418476264413
15	23.742855301576917	31.05476321490361	22.886383803532567	22.315997679986914
16	22.349526451072393	27.41596706350092	27.701410112457026	22.533096372969663
17	21.967781406980365	28.26429915735072	27.267854982617507	22.500064453051404
18	21.87387049704823	26.456535115053743	29.968461922518763	21.701132465379263
19	24.077819020851855	26.239998334191856	27.27954194242154	22.402640702534754
20	24.954475843814343	26.192713061231167	27.68865195450212	21.16415914045237
21	24.07301287669386	25.998023088423665	26.440894904445972	23.488069130436507
22	24.516961984647896	27.699286338291714	26.342317650360304	21.44143402670009
23	22.836344562707822	27.358716469159834	27.586870203727106	22.218068764405242
24	24.02365361963044	26.060278259214165	27.903160558263494	22.012907562891897
25	23.17801737792348	26.840548355928668	27.8475101343128	22.13392413183505
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	311.0
1	311.0
2	625.5
3	940.0
4	940.0
5	940.0
6	1639.5
7	2339.0
8	2339.0
9	2339.0
10	2703.0
11	3067.0
12	3067.0
13	3067.0
14	4340.5
15	5614.0
16	5614.0
17	5614.0
18	11230.5
19	16847.0
20	16847.0
21	16847.0
22	28952.5
23	41058.0
24	41058.0
25	41058.0
26	64540.5
27	88023.0
28	88023.0
29	88023.0
30	115250.0
31	142477.0
32	142477.0
33	142477.0
34	161984.0
35	181491.0
36	181491.0
37	181491.0
38	194915.5
39	208340.0
40	208340.0
41	208340.0
42	223325.5
43	238311.0
44	238311.0
45	238311.0
46	256707.0
47	275103.0
48	275103.0
49	275103.0
50	266771.5
51	258440.0
52	258440.0
53	258440.0
54	231348.0
55	204256.0
56	204256.0
57	204256.0
58	177023.5
59	149791.0
60	149791.0
61	149791.0
62	125693.0
63	101595.0
64	101595.0
65	101595.0
66	78771.5
67	55948.0
68	55948.0
69	55948.0
70	41470.5
71	26993.0
72	26993.0
73	26993.0
74	19273.0
75	11553.0
76	11553.0
77	11553.0
78	7907.5
79	4262.0
80	4262.0
81	4262.0
82	2908.0
83	1554.0
84	1554.0
85	1554.0
86	1006.5
87	459.0
88	459.0
89	459.0
90	288.5
91	118.0
92	118.0
93	118.0
94	71.0
95	24.0
96	24.0
97	24.0
98	17.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32695633911925365
2	0.01129314426892741
3	0.01020345490964494
4	0.01218470829015852
5	0.013472522987492347
6	0.07825950853028643
7	0.07726888184002964
8	0.10228220576901359
9	0.08237060929485211
10	0.08930499612664965
11	0.08608545938331508
12	0.09574406961331879
13	0.08217248395680075
14	0.08732374274613607
15	0.08390608066475014
16	0.08464905068244273
17	0.09668516496906272
18	0.0989140750221405
19	0.09336656555670247
20	0.09227687619742002
21	0.08103326326300545
22	0.09916173169470471
23	0.09227687619742002
24	0.0897507781372652
25	0.08752186808418742
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2018924.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.74047548241817
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.63236014409743	38.09773324748257
2	14.289506303979524	14.786917011538247
3	5.182021478776788	8.043607658160441
4	2.441834257682254	5.053666621669496
5	1.2892136613052965	3.3352263917182623
6	0.7893619679490756	2.4505178129653498
7	0.5021848226036484	1.818829705109661
8	0.34708025607543863	1.436647798392212
9	0.24000968159061248	1.1176393541293868
>10	1.154818177057755	10.318285692264768
>50	0.07322716235708392	2.657078088962849
>100	0.049104778413624074	4.9682486289541
>500	0.005798317569653631	2.182956086355252
>1k	0.003285713289470389	3.141380711011843
>5k	1.932772523217876E-4	0.5912651912856945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6098	0.3020420778592953	No Hit
GTCCTACAGTGGACATTTCTAAATT	5727	0.2836659527550319	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4911	0.24324838379255484	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4671	0.23136086350947338	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3859	0.19114141988504768	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3621	0.17935296227099187	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3337	0.16528606326934547	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3180	0.15750964375082965	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2724	0.13492335521297485	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2146	0.1062942438645536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	4.953133451283951E-5	0.0	0.0	4.953133451283951E-5	0.0
11	4.953133451283951E-5	0.0	0.0	4.953133451283951E-5	0.0
12	4.953133451283951E-5	0.0	0.0	4.953133451283951E-5	0.0
13	4.953133451283951E-5	0.0	0.0	4.953133451283951E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1785	0.0	12.910834	1
TAGGACC	615	0.0	12.509942	4
ACCGACC	55	0.0030719915	12.089331	8
GGTCTAT	120	9.664291E-9	11.903842	1
TTAGACT	160	3.6379788E-12	11.872863	4
TAAGTAG	120	1.00462785E-8	11.872862	5
GTACTAG	115	6.842856E-8	11.593308	1
CCATCGC	110	4.978774E-7	11.225807	19
TTAGGAC	1510	0.0	11.007952	3
GCGCCAC	210	0.0	10.855996	13
AGACCGT	70	0.0014933611	10.855725	6
GTATCAA	3810	0.0	10.672842	1
GGCGAGG	1110	0.0	10.611228	19
CACCTTT	640	0.0	10.537949	14
AATGTCC	610	0.0	10.433065	8
GTCCTAA	1335	0.0	10.414748	1
GGACCTG	1505	0.0	10.413923	6
AGGACCT	1535	0.0	10.395523	5
GTATTAG	165	8.913048E-10	10.388808	1
TAGAAAT	615	0.0	10.34773	4
>>END_MODULE