FastQCFastQC Report
Thu 26 May 2016
SRR1512884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2018924
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT78910.3908517606408166No Hit
TATCAACGCAGAGTACTTTTTTTTT58690.2906994022558551No Hit
GTCCTACAGTGGACATTTCTAAATT52160.2583554408189709No Hit
GTCCTAAAGTGTGTATTTCTCATTT45870.22720023141039486No Hit
GGTATCAACGCAGAGTACTTTTTTT45460.22516944669536842No Hit
CTGTAGGACGTGGAATATGGCAAGA45410.2249217900228042No Hit
CTTTAGGACGTGAAATATGGCGAGG41410.2051092562176684No Hit
GTACTGGTTCACTATCGGTCAGTCA29070.14398758942882445No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26630.1319019438076916No Hit
GTCCTACAGTGTGCATTTCTCATTT24580.12174802023255953No Hit
CTGAAGGACCTGGAATATGGCGAGA21780.10787924656896447No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.012.3701981
TAGGACC6400.012.1824264
TCGCGCG2700.011.6073569
TATCGCG2800.011.5419927
ATCGCGC2750.011.4005518
TCCAACG1604.5474735E-1111.2739718
TGTCGAG1354.778485E-911.25199318
ACCCTAC1657.8216544E-1110.9526821
CGTGTAA700.001495766610.8536329
GGCGAGG11500.010.81395219
CTGCGTG1158.8563866E-710.7356579
CACCTTT6650.010.70763714
TAGGACT2050.010.6677774
TTAGGAC16100.010.4547093
GTCCTAC23900.010.4268751
CCTATAC1201.5043443E-610.3021233
CCCCGGT2400.010.28426219
GTCCTAT1951.4551915E-1110.2431951
CCTACAC3250.010.2411633
ATAGGAC2800.010.1889123
Produced by FastQC (version 0.11.5)