##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512884_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2018924
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.306202709958374	31.0	30.0	34.0	26.0	34.0
2	30.305097170572047	31.0	30.0	34.0	26.0	34.0
3	30.47826317384904	31.0	30.0	34.0	27.0	34.0
4	34.10126829935154	37.0	35.0	37.0	30.0	37.0
5	33.70243505946732	37.0	35.0	37.0	28.0	37.0
6	33.57940566361091	37.0	35.0	37.0	28.0	37.0
7	33.4233230176074	37.0	35.0	37.0	27.0	37.0
8	33.3711546348451	37.0	35.0	37.0	27.0	37.0
9	34.689228024432815	39.0	35.0	39.0	27.0	39.0
10	34.61707275756789	39.0	35.0	39.0	27.0	39.0
11	34.59993342988641	39.0	35.0	39.0	27.0	39.0
12	34.4651205295494	39.0	35.0	39.0	27.0	39.0
13	34.46037691364311	39.0	35.0	39.0	27.0	39.0
14	35.532455902252885	39.0	36.0	41.0	27.0	41.0
15	35.531558889784854	40.0	36.0	41.0	27.0	41.0
16	35.536560563943965	40.0	36.0	41.0	27.0	41.0
17	35.530545973994066	40.0	36.0	41.0	27.0	41.0
18	35.45327808278073	40.0	36.0	41.0	26.0	41.0
19	35.46214914479198	40.0	36.0	41.0	26.0	41.0
20	35.43563749799398	40.0	36.0	41.0	26.0	41.0
21	35.4012389768015	40.0	36.0	41.0	26.0	41.0
22	35.370020862598096	40.0	36.0	41.0	26.0	41.0
23	35.20309580746972	40.0	35.0	41.0	25.0	41.0
24	35.157577501679114	40.0	35.0	41.0	25.0	41.0
25	35.088646724690975	40.0	35.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7563371689012328
1101	2	0.5660512077150734
1101	3	0.5065937434524486
1101	4	0.6071314786287729
1101	5	0.6813812098322529
1101	6	0.6710158484493292
1101	7	0.7279076990538726
1101	8	0.6716679634846656
1101	9	0.8835608371037793
1101	10	0.7145345781535966
1101	11	1.0103494427840474
1101	12	1.0642370770529794
1101	13	1.1032109767873095
1101	14	1.1266451172944159
1101	15	0.7572688153769391
1101	16	1.081675154542424
1101	17	1.1392620186345823
1101	18	1.0077772976563253
1101	19	0.8494501231937832
1101	20	0.9442302247982113
1101	21	0.9511740269468874
1101	22	1.0122834334785722
1101	23	1.0364276907420162
1101	24	1.101759638552025
1101	25	1.1984442387931225
1102	1	0.7167722758408992
1102	2	0.7363214450282989
1102	3	0.4454235439409544
1102	4	0.5676814222442843
1102	5	0.7113434027992227
1102	6	0.6885382498188832
1102	7	0.7490293766236604
1102	8	0.81769822501645
1102	9	1.0178816810208033
1102	10	0.8315789175166515
1102	11	0.975623171323079
1102	12	0.8942571655185745
1102	13	0.9267029766579498
1102	14	1.0171056675031167
1102	15	1.2136827830371075
1102	16	1.095468492000549
1102	17	1.0623386236043686
1102	18	0.5767501880360726
1102	19	0.969613503018472
1102	20	1.0348267127894033
1102	21	0.9387495044919731
1102	22	0.8219229279639251
1102	23	1.0325531458340365
1102	24	0.9328202554571945
1102	25	1.0091254281108704
1103	1	0.5831108036257007
1103	2	0.6917009185299889
1103	3	0.5213639477932688
1103	4	0.3823144523595019
1103	5	0.5469812123285891
1103	6	0.49442338021132315
1103	7	0.679355912794442
1103	8	0.5933510981771519
1103	9	0.637688348978287
1103	10	0.619295665662186
1103	11	0.7309722581637743
1103	12	0.8468576800536809
1103	13	0.9311723480462462
1103	14	0.9007787972025909
1103	15	0.9190041235211837
1103	16	0.9158561504458334
1103	17	0.9067533607643838
1103	18	0.8899128310950246
1103	19	0.9006385618231505
1103	20	0.8738632579989769
1103	21	0.9849699628055788
1103	22	0.8402630071977626
1103	23	0.9891168962150232
1103	24	0.9239766253999662
1103	25	0.8962189100176801
1104	1	0.6667259966251216
1104	2	0.7836532317142471
1104	3	0.6843651538412239
1104	4	0.5624848349781431
1104	5	0.6946061383678241
1104	6	0.6030744235833936
1104	7	0.5795753234242937
1104	8	0.6061148285750946
1104	9	0.6551429377503979
1104	10	0.7875697411942255
1104	11	0.8343900390415797
1104	12	0.8634031221205944
1104	13	0.8352889655367548
1104	14	1.0328402926476343
1104	15	1.0500960035742892
1104	16	0.9096251546202865
1104	17	0.8859385270699036
1104	18	0.5302468964018061
1104	19	1.0461531306950178
1104	20	1.0020302676742716
1104	21	0.9712269420258224
1104	22	0.9695562461636626
1104	23	0.9011978444365951
1104	24	1.1106147387527514
1104	25	0.9244605078180754
1105	1	0.45578276553295893
1105	2	0.5317254663242039
1105	3	0.5624035784120345
1105	4	0.2864052258333274
1105	5	0.31026661439574355
1105	6	0.44041944577236336
1105	7	0.4580785906385998
1105	8	0.4008822619900201
1105	9	0.7213342907088105
1105	10	0.29541396080332305
1105	11	0.702970307923998
1105	12	0.7364620409256588
1105	13	0.6999891904528042
1105	14	0.7841455441589815
1105	15	0.8221000685263675
1105	16	0.7782362919595016
1105	17	0.5728839540593427
1105	18	0.5257313253837808
1105	19	0.7400457762549237
1105	20	0.7377133563599543
1105	21	0.5813967669593012
1105	22	0.6483639005725195
1105	23	0.7520317769811484
1105	24	0.7946079912712563
1105	25	0.7203210477618569
1106	1	-0.1339112773231328
1106	2	0.10889015709435412
1106	3	-1.8389727991928382
1106	4	-0.5700894601095783
1106	5	-0.3673313008902497
1106	6	-0.10602353140868814
1106	7	0.012716220217320995
1106	8	0.08868713504991632
1106	9	0.19110368763082874
1106	10	0.057826109089653244
1106	11	0.11838802799304915
1106	12	0.2427736598517214
1106	13	0.1110143248383153
1106	14	0.24003784757159252
1106	15	0.3625566519844696
1106	16	0.2715857433720785
1106	17	0.2206482864681405
1106	18	0.3064716524907922
1106	19	0.27218866261836894
1106	20	0.3268058348623626
1106	21	0.35615295032904015
1106	22	0.34452460682257424
1106	23	0.3490699776471473
1106	24	0.3819407764262621
1106	25	0.37458477062907747
1107	1	0.5485908755166413
1107	2	0.5263597605768169
1107	3	0.523501820998824
1107	4	0.4185253751290503
1107	5	0.4441091661772276
1107	6	0.34310604979484793
1107	7	0.304648008772908
1107	8	0.3751181217220605
1107	9	0.4958855595348908
1107	10	0.362842423269953
1107	11	0.5387278211624249
1107	12	0.7398904346212305
1107	13	0.5361624560153828
1107	14	0.7013264308055156
1107	15	0.6360016696417077
1107	16	0.7868404894949919
1107	17	0.6359415466970901
1107	18	0.6829661973133057
1107	19	0.62335613380192
1107	20	0.6919454336836353
1107	21	0.6135448935730778
1107	22	0.5585324834510317
1107	23	0.705959854955033
1107	24	0.6624198524037297
1107	25	0.7158845328291576
1108	1	0.43431447790690925
1108	2	0.3256044598215162
1108	3	0.3818927877231246
1108	4	0.25166533326900975
1108	5	0.46647243854049947
1108	6	0.2837565487786833
1108	7	0.369038914830476
1108	8	0.3170494469867222
1108	9	0.5560624030450612
1108	10	0.41707544416964026
1108	11	0.5109667267346651
1108	12	0.5257467454775409
1108	13	0.6450721232583874
1108	14	0.675020579499666
1108	15	0.6016142519209566
1108	16	0.5109122885667929
1108	17	0.6142372666594795
1108	18	0.6792913103050893
1108	19	0.6620630225088107
1108	20	0.6351947435996053
1108	21	0.7407903208185047
1108	22	0.7628724294576443
1108	23	0.8250008656627088
1108	24	0.68965087130141
1108	25	0.7032279368392267
1109	1	0.21630018592301425
1109	2	0.1961551496148637
1109	3	0.3007027749385749
1109	4	0.2954929707983496
1109	5	-0.10605317826641425
1109	6	0.24057243215303714
1109	7	0.2602151889923263
1109	8	0.26462753769979486
1109	9	0.374062383963647
1109	10	-0.11393472622744127
1109	11	0.2520805092446352
1109	12	0.5217038695258509
1109	13	0.3110699861393016
1109	14	0.5059467916468918
1109	15	0.48774587555902116
1109	16	0.40030349584826297
1109	17	0.6126077133555299
1109	18	0.7756238613001827
1109	19	0.7222281722025556
1109	20	0.672097733658191
1109	21	0.7264849799289266
1109	22	0.6592336025833703
1109	23	0.6170214926781838
1109	24	0.6235828842004523
1109	25	0.6219286829227713
1110	1	0.5027885807094457
1110	2	0.2540172878363052
1110	3	0.3467973789851371
1110	4	0.41355494792959746
1110	5	0.49324094004874297
1110	6	0.5592137471487177
1110	7	0.5890121897996679
1110	8	0.619021693779338
1110	9	0.5094011418655313
1110	10	0.6657138662210045
1110	11	0.5533378077564919
1110	12	0.7049902418827045
1110	13	0.6620298546170176
1110	14	0.7091783441228259
1110	15	0.7200140765261267
1110	16	0.687599707767987
1110	17	0.7722937105293539
1110	18	0.6491914410951978
1110	19	0.6570727750660197
1110	20	0.8727997347920677
1110	21	0.9147782095032682
1110	22	0.9007206675712425
1110	23	0.9450342436007588
1110	24	0.9973041716726172
1110	25	0.9283400156459436
1111	1	0.029277265483450776
1111	2	-0.14033359064401907
1111	3	-0.11849630324142524
1111	4	0.11291084375788074
1111	5	0.18091093509759304
1111	6	0.20430001792210817
1111	7	0.01684067479509821
1111	8	-0.03768617704579924
1111	9	0.027801740694428645
1111	10	0.018395036358079153
1111	11	0.05920676211696474
1111	12	0.2734207783739322
1111	13	0.21554810675681324
1111	14	0.09886817632440881
1111	15	-0.03789954641516147
1111	16	-0.036316796602378076
1111	17	0.23816912522176636
1111	18	-0.02940099328118606
1111	19	-0.05126196090573387
1111	20	0.1049762349942327
1111	21	0.22976156507078827
1111	22	-0.1721827410103316
1111	23	-0.10517989673444106
1111	24	0.06448349165081169
1111	25	-0.12240555129168484
1112	1	0.055651776685820664
1112	2	-0.2821719699294363
1112	3	0.016835608918601253
1112	4	0.15511712445965742
1112	5	0.04820499001827727
1112	6	0.09357973259336916
1112	7	0.11302137924818823
1112	8	0.045307740160083654
1112	9	0.15265891614900795
1112	10	-0.05307194009664329
1112	11	-0.08252286537488374
1112	12	0.3446418689905002
1112	13	0.18352065671753337
1112	14	-0.17724747563463694
1112	15	-0.2340691415119096
1112	16	-0.2907432568785637
1112	17	-0.049488454815715954
1112	18	0.12052632754859616
1112	19	0.17427698806672254
1112	20	-0.011558477622827468
1112	21	0.10802315875197621
1112	22	0.17285007467944524
1112	23	0.45310862917386885
1112	24	0.2990901483096948
1112	25	0.33181003728969216
1113	1	-0.6176037175471123
1113	2	-0.504803986007488
1113	3	-0.5000069469024666
1113	4	-2.283320821109669
1113	5	-0.7627245154228106
1113	6	-0.43094865486732914
1113	7	-0.5092378822157855
1113	8	-0.3921434686703691
1113	9	-0.42948911420427294
1113	10	-0.47230381047441483
1113	11	-0.5351334232595661
1113	12	-0.2516589312272046
1113	13	-0.49448597066108135
1113	14	-0.4635221688561728
1113	15	-0.5585680487597884
1113	16	-0.6984075452857539
1113	17	-0.7672672908575393
1113	18	-0.6235701181015116
1113	19	-0.37226546485705825
1113	20	-0.23667845358511386
1113	21	-0.32421487128949167
1113	22	-0.3653611000219925
1113	23	-0.11452549870789142
1113	24	-0.10081007250843754
1113	25	-0.256279389802053
1114	1	-0.9480159448853591
1114	2	-2.1171750666929476
1114	3	-1.6265603441235825
1114	4	-1.1035136408858648
1114	5	-0.8455655030155143
1114	6	-0.40939905662592224
1114	7	-0.5421667687647655
1114	8	-0.5672126263315818
1114	9	-0.8970239162059102
1114	10	-1.1107151978780294
1114	11	-0.9895203064832927
1114	12	-1.0508277205547571
1114	13	-1.1142882411220612
1114	14	-1.2893673023581016
1114	15	-1.3246344867775832
1114	16	-1.56166902768922
1114	17	-1.8241802673403882
1114	18	-1.5185302059903236
1114	19	-2.126462551257731
1114	20	-1.3765657116347398
1114	21	-1.5098084707441402
1114	22	-1.151937159247531
1114	23	-1.0107818045577162
1114	24	-1.1158430764616085
1114	25	-1.858121565273322
1115	1	-0.968709752791451
1115	2	-1.1154679558777651
1115	3	-0.6371117267415869
1115	4	-0.3333609243318776
1115	5	-0.6780480147486116
1115	6	-0.5559085516451248
1115	7	-0.38967215942786027
1115	8	-0.6343210155155816
1115	9	-1.6311565945595063
1115	10	-1.246209980558568
1115	11	-1.7190037910468021
1115	12	-2.2902911207468932
1115	13	-2.3802964044733272
1115	14	-1.7814401722969109
1115	15	-1.9164044508614282
1115	16	-1.699950066325414
1115	17	-1.5811929894672971
1115	18	-1.8287771437210765
1115	19	-2.392649525860456
1115	20	-2.464049774550382
1115	21	-1.7351217853174816
1115	22	-1.8318115580124186
1115	23	-1.6601060073794898
1115	24	-2.1018614743776
1115	25	-2.3788040329414954
1116	1	-0.8527552364584139
1116	2	-1.0588265858939643
1116	3	-1.0533863319762844
1116	4	-0.6258914884049673
1116	5	-1.0519692524036799
1116	6	-1.804463837776698
1116	7	-1.4598465116957264
1116	8	-1.6032802559876345
1116	9	-2.527296641916479
1116	10	-1.235673548876953
1116	11	-2.257755436074234
1116	12	-4.39080590906881
1116	13	-3.012797069089178
1116	14	-2.446286472657043
1116	15	-3.701450656111316
1116	16	-3.238773332999017
1116	17	-2.7803630865424935
1116	18	-2.621782227000331
1116	19	-3.0701655303055375
1116	20	-4.942201457225622
1116	21	-3.794958471452027
1116	22	-2.9078646645342374
1116	23	-3.4675721615076185
1116	24	-3.5835450123795916
1116	25	-2.6540704778493804
1201	1	0.5638621483150601
1201	2	0.7533456758648072
1201	3	0.8471387263237844
1201	4	0.7076221538605765
1201	5	0.2049920539110559
1201	6	0.7173976100439603
1201	7	0.9409378703835998
1201	8	0.8232317316076347
1201	9	0.7223814285529713
1201	10	0.9548577312280031
1201	11	0.5771806372559141
1201	12	1.1364200298181473
1201	13	1.0716019271656165
1201	14	1.0755013663081883
1201	15	0.9157173316377225
1201	16	0.8347880152312683
1201	17	0.765366414892938
1201	18	1.0970469072972762
1201	19	1.2318218978698567
1201	20	1.141717661713514
1201	21	0.9277048760450057
1201	22	0.9344030255357865
1201	23	1.044318562760921
1201	24	0.6872344453328338
1201	25	0.9206270569201536
1202	1	0.6038098427424536
1202	2	0.646050908878987
1202	3	0.6667055294943509
1202	4	0.5639155113897445
1202	5	0.5934483711082166
1202	6	0.5252820270882026
1202	7	0.6683058020850865
1202	8	0.522515137108968
1202	9	0.7231820196728833
1202	10	0.76409227406333
1202	11	0.6415199677269854
1202	12	0.7527688686767107
1202	13	0.7505193975079649
1202	14	0.8752820245116766
1202	15	0.8647374229128388
1202	16	0.8595419665003519
1202	17	0.7042935004826916
1202	18	0.7073313381603512
1202	19	0.8446340594733925
1202	20	0.6587319396370788
1202	21	0.8425147710594203
1202	22	0.717617284082948
1202	23	0.9630709023334418
1202	24	0.8940017470113801
1202	25	1.0231800756969207
1203	1	0.6976389359136519
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2211	8	-0.11333016582048572
2211	9	0.16451527453362758
2211	10	0.12095706973022402
2211	11	0.20291116128356634
2211	12	0.013754238382432504
2211	13	0.19081585245300658
2211	14	0.20093284957673063
2211	15	0.22469421978023263
2211	16	0.26960720502862046
2211	17	0.026108751267948094
2211	18	-0.040366778905209344
2211	19	0.07833396642482882
2211	20	0.1283734491834636
2211	21	0.18531670035610404
2211	22	0.27445536004238846
2211	23	0.044788883348019226
2211	24	0.3085465089503927
2211	25	0.04844284823454359
2212	1	-0.33965632764573783
2212	2	-0.22926865080088632
2212	3	-0.1758849318752027
2212	4	-0.18775677026713566
2212	5	-0.4999027490882213
2212	6	-0.2883411694233402
2212	7	-0.5442222178070253
2212	8	-0.3515234693163265
2212	9	-0.37984042563972764
2212	10	-0.6457345068432616
2212	11	-0.37601562162060276
2212	12	-0.1477993500996604
2212	13	-0.19792656188607083
2212	14	-0.19776365619691916
2212	15	-0.3571516456133992
2212	16	-0.2854878580454212
2212	17	-0.43530602466829293
2212	18	-0.5025345935126495
2212	19	-0.22319285070653905
2212	20	-0.32668229995352505
2212	21	-0.20792682233784632
2212	22	-0.27190491779221304
2212	23	-0.7907299845717546
2212	24	-0.5504752035638987
2212	25	-0.46279002897452415
2213	1	-0.5275688234306699
2213	2	-0.03086882762522336
2213	3	-0.13323847290961055
2213	4	-0.2767504086423074
2213	5	-0.21703882240439043
2213	6	-0.16461556965243318
2213	7	-0.5892038939285555
2213	8	-0.5290599472790163
2213	9	-0.20541197693964364
2213	10	-0.2451344479812576
2213	11	-0.09375928035122882
2213	12	-0.5046566351470787
2213	13	-0.3291229103761779
2213	14	-0.35787964056869725
2213	15	-0.602979870224793
2213	16	-0.9875882030752905
2213	17	-0.6183570427784915
2213	18	-0.34420293826291015
2213	19	-0.24576228067047623
2213	20	-0.2561472832143963
2213	21	-0.18367940807600291
2213	22	-0.3954488079196423
2213	23	-0.552420666699831
2213	24	-0.4295321444155249
2213	25	-0.2642963721305094
2214	1	-1.0562233037138817
2214	2	-0.4017553903009805
2214	3	-0.43855804573030355
2214	4	-0.4634413103395758
2214	5	-0.6676246936111028
2214	6	-1.3614637038994175
2214	7	-0.6849806364977624
2214	8	-0.6091659058499062
2214	9	-0.449652877735069
2214	10	-0.7240784204602306
2214	11	-0.7605325286815798
2214	12	-0.46953715710208144
2214	13	-0.8170886107570823
2214	14	-0.43501688987633
2214	15	-0.5816422211539347
2214	16	-0.6905684723497671
2214	17	-0.7470707988638949
2214	18	-0.7024087425363419
2214	19	-0.8380054311588694
2214	20	-0.9465521459618103
2214	21	-1.0515140458049288
2214	22	-1.3075796049373238
2214	23	-1.3491800922082646
2214	24	-1.0382265846240344
2214	25	-1.4719654147484178
2215	1	-1.2874319901705817
2215	2	-0.6982525046121886
2215	3	-0.7015316888597702
2215	4	-0.8047846322969363
2215	5	-1.1963743824374262
2215	6	-1.0929810092436725
2215	7	-1.387973279105239
2215	8	-1.1784555469240985
2215	9	-1.0873409462018628
2215	10	-1.0848343814033825
2215	11	-0.862893544924745
2215	12	-1.1342137429024248
2215	13	-1.1135974771120232
2215	14	-1.262571487827671
2215	15	-1.0250735031731466
2215	16	-1.1208496262001617
2215	17	-1.3385643425976426
2215	18	-1.2143362493903354
2215	19	-1.231255895211305
2215	20	-1.1381718759877302
2215	21	-0.7610446058194498
2215	22	-1.1823653600705128
2215	23	-1.7115569154612018
2215	24	-1.6175603398046405
2215	25	-1.776268236506084
2216	1	-1.3107446997280263
2216	2	-0.7709520140076833
2216	3	-0.7994637065121566
2216	4	-1.128079080566316
2216	5	-1.8621930037613055
2216	6	-2.03648118206511
2216	7	-2.064495928552848
2216	8	-2.1367129241998946
2216	9	-3.9865276387874005
2216	10	-2.0197116183749984
2216	11	-2.0864365994565404
2216	12	-2.4383104935796993
2216	13	-2.3031117355315374
2216	14	-2.4301676665976757
2216	15	-2.2962839217347977
2216	16	-1.739156581956621
2216	17	-2.0753658350648365
2216	18	-2.5289833555453356
2216	19	-2.650586196958592
2216	20	-2.042815153349686
2216	21	-2.6182240654686098
2216	22	-2.107630580439306
2216	23	-3.1547996648953998
2216	24	-2.557382594671445
2216	25	-2.5650865208691656
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	51539.0
3	0.0
4	836.0
5	7486.0
6	9349.0
7	7175.0
8	5895.0
9	4962.0
10	4199.0
11	3880.0
12	3585.0
13	3264.0
14	3174.0
15	3166.0
16	3313.0
17	3542.0
18	3793.0
19	4731.0
20	6259.0
21	8378.0
22	10339.0
23	12657.0
24	14924.0
25	17198.0
26	19195.0
27	21413.0
28	24140.0
29	29107.0
30	36323.0
31	46630.0
32	58885.0
33	75816.0
34	98474.0
35	128780.0
36	176987.0
37	274557.0
38	834973.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.74663816514162	19.170811847301362	13.548271318390523	26.5342786691665
2	13.612385400655464	20.34994980471905	40.54782723309539	25.4898375615301
3	17.734273480604138	25.481373006055343	29.23302827922433	27.55132523411619
4	11.845325574449179	16.829011807234526	36.701889742016114	34.62377287630019
5	12.930705223375972	38.918587145682956	34.51497223126297	13.6357353996781
6	32.21884603928476	36.18414564096566	18.027049222170074	13.569959097579506
7	28.61098114165173	31.574697854050026	21.439376516677505	18.37494448762074
8	26.04099770869507	36.424910210974474	20.030980186951457	17.503111893379
9	27.50817029485389	14.372585261119998	19.665430326965367	38.453814117060745
10	16.222289723493102	28.713834858966663	32.85239503703788	22.21148038050235
11	34.61803084046738	22.103820954696285	24.33599278001586	18.942155424820477
12	24.452169158018876	24.53627365562824	30.604873801151804	20.406683385201074
13	29.598098278087697	21.72001018425779	25.6291602643548	23.05273127329971
14	21.55001998610136	22.26704304268209	27.085204192573887	29.09773277864266
15	23.602197092340095	31.003810433414507	22.962914159237677	22.431078315007717
16	22.25692471617295	27.485910814975824	27.60167569289267	22.655488775958553
17	21.66930269627936	28.36222563786055	27.447829646937144	22.520642018922945
18	21.58662776586207	26.452415036580955	30.168812320129184	21.79214487742779
19	23.96684103718842	26.168159490331526	27.544664593901274	22.320334878578777
20	24.697210152895746	26.10793445117421	27.87841053409494	21.316444861835105
21	23.65305126763633	25.843980553911383	26.958638674733702	23.54432950371858
22	24.21413117560349	27.523438366803504	26.738163921954005	21.524266535638997
23	22.53482548129598	27.1298473840521	27.92234873625753	22.412978398394394
24	23.89337092431414	25.999096548458482	28.108487491356783	21.999045035870594
25	22.81042912483537	26.806718235415616	28.188445027373504	22.194407612375507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	127.0
1	127.0
2	337.0
3	547.0
4	547.0
5	547.0
6	1371.5
7	2196.0
8	2196.0
9	2196.0
10	2512.5
11	2829.0
12	2829.0
13	2829.0
14	4205.5
15	5582.0
16	5582.0
17	5582.0
18	11442.0
19	17302.0
20	17302.0
21	17302.0
22	30051.5
23	42801.0
24	42801.0
25	42801.0
26	67539.5
27	92278.0
28	92278.0
29	92278.0
30	119074.0
31	145870.0
32	145870.0
33	145870.0
34	164423.5
35	182977.0
36	182977.0
37	182977.0
38	195932.5
39	208888.0
40	208888.0
41	208888.0
42	224142.0
43	239396.0
44	239396.0
45	239396.0
46	257202.5
47	275009.0
48	275009.0
49	275009.0
50	266118.5
51	257228.0
52	257228.0
53	257228.0
54	229892.0
55	202556.0
56	202556.0
57	202556.0
58	175035.0
59	147514.0
60	147514.0
61	147514.0
62	123284.0
63	99054.0
64	99054.0
65	99054.0
66	76573.5
67	54093.0
68	54093.0
69	54093.0
70	40088.5
71	26084.0
72	26084.0
73	26084.0
74	18401.5
75	10719.0
76	10719.0
77	10719.0
78	7366.5
79	4014.0
80	4014.0
81	4014.0
82	2687.5
83	1361.0
84	1361.0
85	1361.0
86	886.0
87	411.0
88	411.0
89	411.0
90	246.5
91	82.0
92	82.0
93	82.0
94	44.0
95	6.0
96	6.0
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08529295803110963
2	0.03992225561734865
3	0.018574250442314817
4	0.0038139127574886426
5	0.014215493005184942
6	0.05918994474284322
7	0.06716448959941038
8	0.04259694768104198
9	0.01639487172374988
10	0.04234929100847778
11	0.004111100764565679
12	3.467193415898766E-4
13	0.00604282281056642
14	0.0010401580247696297
15	0.012680021635286915
16	0.008767046208772594
17	0.005200790123848149
18	0.005646572134463705
19	0.002129847384052099
20	0.0015850027044108643
21	4.4578201061555567E-4
22	5.943760141540742E-4
23	0.0
24	0.0
25	4.953133451283951E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2018924.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.88170792137237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.87929579865641	37.08223042342536
2	14.652638436640064	14.911025384211879
3	5.361340208278474	8.183824396342049
4	2.5096298478865475	5.107770116436859
5	1.351489927033096	3.438305786298742
6	0.798380824610728	2.4373787956720485
7	0.5216103188188331	1.857829672363965
8	0.3659279009829459	1.4895229262476095
9	0.25290535486958504	1.1581430758402729
>10	1.1718491590129159	10.363047935053697
>50	0.07344598991678795	2.6134828758654343
>100	0.052509227431434054	5.247109252074676
>500	0.00497638368447201	1.8374877276699848
>1k	0.0037078937256850263	3.3307133666162816
>5k	2.9272845202776523E-4	0.9421282658812652
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7891	0.3908517606408166	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5869	0.2906994022558551	No Hit
GTCCTACAGTGGACATTTCTAAATT	5216	0.2583554408189709	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4587	0.22720023141039486	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4546	0.22516944669536842	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4541	0.2249217900228042	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4141	0.2051092562176684	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2907	0.14398758942882445	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2663	0.1319019438076916	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2458	0.12174802023255953	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2178	0.10787924656896447	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.953133451283951E-5	0.0
10	0.0	0.0	0.0	4.953133451283951E-5	0.0
11	0.0	0.0	0.0	4.953133451283951E-5	0.0
12	0.0	0.0	0.0	4.953133451283951E-5	0.0
13	0.0	0.0	0.0	4.953133451283951E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1830	0.0	12.370198	1
TAGGACC	640	0.0	12.182426	4
TCGCGCG	270	0.0	11.607356	9
TATCGCG	280	0.0	11.541992	7
ATCGCGC	275	0.0	11.400551	8
TCCAACG	160	4.5474735E-11	11.27397	18
TGTCGAG	135	4.778485E-9	11.251993	18
ACCCTAC	165	7.8216544E-11	10.952682	1
CGTGTAA	70	0.0014957666	10.853632	9
GGCGAGG	1150	0.0	10.813952	19
CTGCGTG	115	8.8563866E-7	10.735657	9
CACCTTT	665	0.0	10.707637	14
TAGGACT	205	0.0	10.667777	4
TTAGGAC	1610	0.0	10.454709	3
GTCCTAC	2390	0.0	10.426875	1
CCTATAC	120	1.5043443E-6	10.302123	3
CCCCGGT	240	0.0	10.284262	19
GTCCTAT	195	1.4551915E-11	10.243195	1
CCTACAC	325	0.0	10.241163	3
ATAGGAC	280	0.0	10.188912	3
>>END_MODULE