FastQCFastQC Report
Thu 26 May 2016
SRR1512883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361492
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65290.47954743766397456No Hit
TATCAACGCAGAGTACTTTTTTTTT36180.26573788167686624No Hit
GTACTGGTTCACTATCGGTCAGTCA35870.2634609678205968No Hit
GGTATCAACGCAGAGTACTTTTTTT35220.25868679360583835No Hit
GGATACCACGTGTCCCGCCCTACTC24780.18200621083340923No Hit
GTCCTACAGTGGACATTTCTAAATT23370.17164992522908692No Hit
GTCCTAAAGTGTGTATTTCTCATTT22540.16555367200101068No Hit
CTGTAGGACGTGGAATATGGCAAGA22080.16217502563364308No Hit
CTTTAGGACGTGAAATATGGCGAGG18150.1333096338428724No Hit
ATCATTAACTGAATCCATAGGTTAA17430.1280213177896014No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17380.12765407361923536No Hit
GTATTTAGCCTTGGAGGATGGTCCC16500.12119057622079307No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13880.1019469816936126No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCGC402.7636113E-416.6227828
CGAGCAA350.002172482216.28294210
CCGAATG1207.421477E-1012.66404518
GGTATCA16400.012.491181
AAGCACG701.09281755E-412.21175718
AATCGCC550.00307286812.08840718
TAAAACG802.8707409E-511.87297912
GTAGTAA657.825546E-411.7269611
GTATCGT752.0767341E-411.39805910
AGCACGA752.0774434E-411.3976419
TAGGACC3700.011.295794
GGACCTA951.36175095E-510.9985316
TAGAACC700.001492856810.8556934
CCTACAC1408.360075E-910.8556933
GGACCGG700.001492856810.8556936
CGGACTG803.7746746E-410.6860735
GTCCTAA7000.010.4809711
GGCGAGG5900.010.46393519
ACGAAGG1002.4032528E-510.44783716
TGTCCCG5000.010.25825311
Produced by FastQC (version 0.11.5)