##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512883_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1361492 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.804089190388193 31.0 30.0 33.0 22.0 34.0 2 28.951327661124708 31.0 30.0 34.0 22.0 34.0 3 29.15561971719261 31.0 30.0 34.0 22.0 34.0 4 32.558767146630316 35.0 33.0 37.0 25.0 37.0 5 32.79337520896193 35.0 33.0 37.0 26.0 37.0 6 32.66852467733927 37.0 33.0 37.0 25.0 37.0 7 32.580860555919536 37.0 33.0 37.0 25.0 37.0 8 32.53327893223023 37.0 33.0 37.0 23.0 37.0 9 33.812713552485064 39.0 34.0 39.0 22.0 39.0 10 33.67146924109727 38.0 34.0 39.0 19.0 39.0 11 33.755400692769406 39.0 34.0 39.0 22.0 39.0 12 33.64557485464476 38.0 34.0 39.0 19.0 39.0 13 33.621053226901076 38.0 34.0 39.0 19.0 39.0 14 34.467508439271036 39.0 34.0 41.0 17.0 41.0 15 34.63429384821946 39.0 34.0 41.0 18.0 41.0 16 34.593764047089515 39.0 34.0 41.0 18.0 41.0 17 34.362305470762955 39.0 34.0 41.0 17.0 41.0 18 34.375414618668344 39.0 34.0 41.0 17.0 41.0 19 34.47817541344349 39.0 34.0 41.0 18.0 41.0 20 34.455680973520224 39.0 34.0 41.0 18.0 41.0 21 34.35433627226601 39.0 34.0 41.0 17.0 41.0 22 34.37433124836576 39.0 34.0 41.0 17.0 41.0 23 34.04475310908914 39.0 34.0 41.0 10.0 41.0 24 34.103986655815824 39.0 34.0 41.0 2.0 41.0 25 33.82281864307686 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5884619324300502 1101 2 0.22922778490525175 1101 3 0.5452572209660786 1101 4 0.6652117345457356 1101 5 0.5998090062434542 1101 6 0.6460290026394588 1101 7 0.3556889676544728 1101 8 0.6464892084918219 1101 9 0.6608941647936248 1101 10 0.7046434411986269 1101 11 0.6834467046440622 1101 12 0.61640963522796 1101 13 1.0355306724519622 1101 14 1.1776964324716346 1101 15 0.9992095114585737 1101 16 0.8172774427236575 1101 17 0.21284240208787963 1101 18 0.7042718223056568 1101 19 0.07030317003211906 1101 20 -0.585481057113924 1101 21 0.5573087492629938 1101 22 0.6983309588968112 1101 23 0.24038039523452426 1101 24 0.7569724645362115 1101 25 0.49405288889646926 1102 1 0.9053030812128391 1102 2 0.8702532602990374 1102 3 0.7814956707667662 1102 4 0.9779402443923857 1102 5 0.8555950514063966 1102 6 0.8067226533852647 1102 7 0.8682307220646166 1102 8 0.9307479214726868 1102 9 1.0994170312096614 1102 10 1.0596775839969155 1102 11 0.8739480159514486 1102 12 1.0821943453981646 1102 13 0.9287807768828458 1102 14 1.2421478724694524 1102 15 1.1414580124626923 1102 16 1.1609031394678553 1102 17 1.2463155507902783 1102 18 1.3442888757613503 1102 19 0.9781585257215539 1102 20 1.3041035591761485 1102 21 1.426959627128383 1102 22 1.1991247771626732 1102 23 1.0690613506299798 1102 24 0.42774315728260603 1102 25 0.8484303013934351 1103 1 0.7181153634128741 1103 2 0.8269877578498637 1103 3 0.9597652469955236 1103 4 1.057148262375918 1103 5 0.8698385554458241 1103 6 0.6260578910878465 1103 7 0.5401245878360328 1103 8 0.8707995554018737 1103 9 1.077423529624319 1103 10 1.1398721437833146 1103 11 0.9460637309243083 1103 12 1.0945432965226303 1103 13 1.0931406045079797 1103 14 1.1759465001456277 1103 15 1.0639285879944893 1103 16 1.1712888233229322 1103 17 0.8724652891849161 1103 18 1.0707664797899739 1103 19 1.001138569109763 1103 20 1.0306400612228543 1103 21 1.0335454071835528 1103 22 0.9103527026763132 1103 23 0.4148663392921108 1103 24 0.6700790526797249 1103 25 0.8099600792497412 1104 1 0.8479992755317447 1104 2 0.8593794879493295 1104 3 0.9224063944504266 1104 4 1.1034745350632207 1104 5 0.7955832448008238 1104 6 0.756018294687216 1104 7 0.9363442548293364 1104 8 0.5848895617554888 1104 9 1.006860483416574 1104 10 1.0143572045147877 1104 11 0.822313608606855 1104 12 1.0960490071973936 1104 13 1.1493735575890653 1104 14 1.2814034437114543 1104 15 1.1388516485909008 1104 16 1.159886768940929 1104 17 1.3223957577400256 1104 18 1.2593634606505617 1104 19 0.8847833123533633 1104 20 0.9319267719651521 1104 21 1.075008613403206 1104 22 0.8905365854389942 1104 23 1.39367199718086 1104 24 1.1627544765872386 1104 25 0.5749444846352958 1105 1 0.48310204956333536 1105 2 0.6565535550364956 1105 3 0.7543552443072485 1105 4 0.970033454942012 1105 5 0.7044854421809319 1105 6 0.8274384143829678 1105 7 0.703414658211976 1105 8 0.7256981198264825 1105 9 0.43185903797817815 1105 10 0.6150146212721026 1105 11 0.5436265865819578 1105 12 0.8692642045143018 1105 13 0.9246780296634185 1105 14 0.9824847660926253 1105 15 0.91962144650293 1105 16 0.8760425571709831 1105 17 1.2219108971945758 1105 18 1.0569059464550108 1105 19 0.7413953750634263 1105 20 0.9805636451210944 1105 21 0.8526865177371477 1105 22 0.8861469346882842 1105 23 1.0373030818602444 1105 24 1.010871843495977 1105 25 1.4002501444342244 1106 1 0.6863905282982081 1106 2 0.73999515221319 1106 3 0.6828068005455528 1106 4 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1107 17 1.3540008306006968 1107 18 1.2423872741652602 1107 19 1.1227842775818218 1107 20 1.1903539335115738 1107 21 1.1828407780358106 1107 22 1.1104590943994666 1107 23 1.3778926675879006 1107 24 1.2754816341302089 1107 25 1.5026889516193336 1108 1 0.9726437501993281 1108 2 0.9284431722085031 1108 3 0.9415508449101537 1108 4 1.0149257298308 1108 5 0.994887819204763 1108 6 1.164075548460346 1108 7 1.1258155993334924 1108 8 1.1612974684804342 1108 9 1.223289252909403 1108 10 1.2588468369515553 1108 11 1.0812993435792748 1108 12 1.198749723309703 1108 13 1.2045511772088702 1108 14 1.2143256836861056 1108 15 1.1471509808943026 1108 16 0.9574422159279337 1108 17 1.3096404199084333 1108 18 1.4698727498639812 1108 19 1.4055637735349649 1108 20 1.6713094276986737 1108 21 1.490011760424096 1108 22 1.4005511541650861 1108 23 1.7306369486111066 1108 24 1.5300567094315767 1108 25 1.620369860627271 1109 1 0.7002465050109272 1109 2 0.7168267111079345 1109 3 0.8134338924840243 1109 4 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14 0.23160678967688852 2211 15 -0.11751424003277577 2211 16 -0.15838863254048619 2211 17 0.33098766848528527 2211 18 -0.13828664292538662 2211 19 -0.14629309824600512 2211 20 -0.06134169726744432 2211 21 -0.012441478936160877 2211 22 0.012492059559498614 2211 23 0.203637761839623 2211 24 -0.11827617210813912 2211 25 0.4510556833434478 2212 1 -0.06802503683540806 2212 2 0.10406000172708474 2212 3 -0.33040378176271545 2212 4 -0.1655150432749437 2212 5 -0.2085126307891798 2212 6 -0.3601926802249622 2212 7 -0.21270902391874102 2212 8 -0.08778650276924793 2212 9 -0.025644295435206743 2212 10 -0.310551875105439 2212 11 -0.3341559725319101 2212 12 -0.17520416824558538 2212 13 -0.05692356215369898 2212 14 -0.03430659392488877 2212 15 -0.5053045455539973 2212 16 -0.5415997941300006 2212 17 -0.18289367488774388 2212 18 -0.06545644896407765 2212 19 -0.30171410303751145 2212 20 -0.0787346581103705 2212 21 -0.039325167761710134 2212 22 0.037153211219560944 2212 23 0.026998329017459355 2212 24 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-1.3331054958609982 2214 11 -1.1816850634911518 2214 12 -1.1287827601367653 2214 13 -1.5033678253337897 2214 14 -1.3104313195655237 2214 15 -1.7774769200776817 2214 16 -1.6769431915757522 2214 17 -3.661685744966121 2214 18 -3.524551782194955 2214 19 -2.1953316273604315 2214 20 -1.9664492691998134 2214 21 -2.052929845958367 2214 22 -2.724543265552988 2214 23 -1.7367413123155444 2214 24 -2.6704754545414495 2214 25 -2.6773242413679093 2215 1 -1.7722723779962024 2215 2 -3.37983000366604 2215 3 -1.9814195076254535 2215 4 -3.5336856108002195 2215 5 -2.3043432251817144 2215 6 -2.2428481898018937 2215 7 -2.117227088580556 2215 8 -1.7394363444391097 2215 9 -3.97466054742571 2215 10 -2.3926049955935085 2215 11 -2.035793184205417 2215 12 -1.9453729748961415 2215 13 -1.9785730082859807 2215 14 -1.9339482452461638 2215 15 -2.088523455515201 2215 16 -1.9908419629567433 2215 17 -2.335337835549666 2215 18 -2.01525749774558 2215 19 -2.1215226670330907 2215 20 -1.9506127103158946 2215 21 -2.5325260022832268 2215 22 -2.4578534930378275 2215 23 -2.2387949739243567 2215 24 -2.19600871036673 2215 25 -3.139055179797289 2216 1 -3.086576991279479 2216 2 -3.2993249184628155 2216 3 -4.723548586318309 2216 4 -5.245974340746688 2216 5 -4.938995603388335 2216 6 -3.8801184150884573 2216 7 -5.512925215213773 2216 8 -3.4503436657413467 2216 9 -4.042997540796748 2216 10 -3.165797161885994 2216 11 -3.3659398310792525 2216 12 -3.8150793130999823 2216 13 -3.322054460687088 2216 14 -4.324336250137119 2216 15 -3.8817671077012044 2216 16 -4.153320851286747 2216 17 -3.8271512104315875 2216 18 -3.5749688659453724 2216 19 -4.04160482363363 2216 20 -4.821807191224398 2216 21 -4.355668482030339 2216 22 -3.986157443833836 2216 23 -4.006926882501112 2216 24 -4.4402696076689345 2216 25 -4.586054766765102 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 66736.0 3 3.0 4 576.0 5 3747.0 6 4833.0 7 4467.0 8 3777.0 9 3552.0 10 3146.0 11 2853.0 12 2804.0 13 2578.0 14 2576.0 15 2573.0 16 2863.0 17 2924.0 18 3413.0 19 3943.0 20 5148.0 21 6891.0 22 8550.0 23 10145.0 24 11684.0 25 13317.0 26 14712.0 27 16192.0 28 17994.0 29 21251.0 30 25743.0 31 32002.0 32 40029.0 33 50780.0 34 66231.0 35 88056.0 36 119623.0 37 187966.0 38 507814.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.73719914149931 18.19176818122855 12.114971874668333 23.956060802603805 2 15.999080322651022 19.98204719296403 40.41467752160177 23.604194962783183 3 19.84383321151932 25.6745986564955 28.188031865076596 26.29353626690858 4 12.906429488069449 16.168957429350705 37.01148209444098 33.91313098813887 5 13.809330020876704 38.43105912911382 33.64344763855241 14.116163211457064 6 32.24371164302553 35.11593187646969 18.425975679408648 14.214380801096137 7 29.324806586177115 31.670911663634527 20.82091993310914 18.18336181707922 8 26.80218780765586 34.56656689334401 20.49007303230759 18.14117226669255 9 28.47783036135651 14.25152200791841 19.39422541154124 37.876422219183844 10 17.53751285593196 27.652144762756137 31.99835032387024 22.811992057441664 11 35.14571300239431 22.339303291673744 23.47499946703364 19.03998423889831 12 24.895546836114953 24.466926779490052 29.777698376463686 20.85982800793131 13 29.986540495759634 21.49176294512477 25.234181407065837 23.28751515204976 14 22.375348632742146 22.35998453298283 26.723315352190745 28.54135148208428 15 24.783456622592258 29.589275120906294 23.05493214618874 22.57233611031271 16 23.066439172346062 27.29828930837953 26.496423286459798 23.13884823281461 17 22.4650206336171 27.770576822268573 26.448231420003104 23.316171124111225 18 22.388634707725156 25.96863119338363 28.933250302725966 22.70948379616525 19 24.079920129862494 26.225180266547472 27.258478115111807 22.436421488478235 20 25.2956467912233 25.409891504280868 27.3513899773422 21.94307172715363 21 24.793276648733123 25.603410191025684 26.483302986710456 23.120010173530737 22 24.96595848552758 26.86493091704752 26.114253027709644 22.05485756971526 23 23.682562181300618 26.452857487340246 27.152819846259714 22.711760485099425 24 24.217266488515104 25.808941640048076 27.32305837465494 22.65073349678188 25 23.680494243553447 26.162959521458856 27.436795238700846 22.71975099628685 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 229.0 1 229.0 2 509.5 3 790.0 4 790.0 5 790.0 6 1487.0 7 2184.0 8 2184.0 9 2184.0 10 2364.5 11 2545.0 12 2545.0 13 2545.0 14 3069.5 15 3594.0 16 3594.0 17 3594.0 18 7325.0 19 11056.0 20 11056.0 21 11056.0 22 18265.5 23 25475.0 24 25475.0 25 25475.0 26 40104.0 27 54733.0 28 54733.0 29 54733.0 30 69579.0 31 84425.0 32 84425.0 33 84425.0 34 97242.0 35 110059.0 36 110059.0 37 110059.0 38 118965.0 39 127871.0 40 127871.0 41 127871.0 42 141261.5 43 154652.0 44 154652.0 45 154652.0 46 173148.5 47 191645.0 48 191645.0 49 191645.0 50 187501.5 51 183358.0 52 183358.0 53 183358.0 54 164736.5 55 146115.0 56 146115.0 57 146115.0 58 127300.5 59 108486.0 60 108486.0 61 108486.0 62 92756.0 63 77026.0 64 77026.0 65 77026.0 66 59316.0 67 41606.0 68 41606.0 69 41606.0 70 31421.5 71 21237.0 72 21237.0 73 21237.0 74 15298.0 75 9359.0 76 9359.0 77 9359.0 78 6402.0 79 3445.0 80 3445.0 81 3445.0 82 2285.5 83 1126.0 84 1126.0 85 1126.0 86 739.5 87 353.0 88 353.0 89 353.0 90 223.5 91 94.0 92 94.0 93 94.0 94 57.0 95 20.0 96 20.0 97 20.0 98 14.5 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3457971108166629 2 0.01065008094061515 3 0.009328001927297406 4 0.011384569281347227 5 0.012192506456152516 6 0.07939818963313776 7 0.07836990595611285 8 0.10348940720914997 9 0.08211579649384647 10 0.0899748217396797 11 0.08674307304045856 12 0.096438319138122 13 0.08189544999162683 14 0.08652272653823893 15 0.08380511967753024 16 0.08556789169528722 17 0.09790729581958615 18 0.0992293748329039 19 0.09430830294999898 20 0.09210483792780273 21 0.0807202686464555 22 0.1019469816936126 23 0.09372071227741331 24 0.09012171940782612 25 0.08747756138119063 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1361492.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.06288544872509 #Duplication Level Percentage of deduplicated Percentage of total 1 73.34642659934516 36.719337529163404 2 14.293386484780553 14.31136340523849 3 5.2091253516110685 7.823515372972643 4 2.4834509068781987 4.97314873074303 5 1.3650201745567683 3.4168424317017116 6 0.8032062913221533 2.4126494732493766 7 0.5179414900819455 1.8150751840980097 8 0.34945815749350145 1.3995906966175757 9 0.2671527926313601 1.203699568932971 >10 1.2115297824769622 10.496487472147546 >50 0.0796859895913862 2.770242891160223 >100 0.06216463338683835 6.126367808787581 >500 0.007138501305484258 2.4894819874199556 >1k 0.004164125761532483 3.5560943913969356 >5k 1.487187771975887E-4 0.486103056370634 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6529 0.47954743766397456 No Hit TATCAACGCAGAGTACTTTTTTTTT 3618 0.26573788167686624 No Hit GTACTGGTTCACTATCGGTCAGTCA 3587 0.2634609678205968 No Hit GGTATCAACGCAGAGTACTTTTTTT 3522 0.25868679360583835 No Hit GGATACCACGTGTCCCGCCCTACTC 2478 0.18200621083340923 No Hit GTCCTACAGTGGACATTTCTAAATT 2337 0.17164992522908692 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2254 0.16555367200101068 No Hit CTGTAGGACGTGGAATATGGCAAGA 2208 0.16217502563364308 No Hit CTTTAGGACGTGAAATATGGCGAGG 1815 0.1333096338428724 No Hit ATCATTAACTGAATCCATAGGTTAA 1743 0.1280213177896014 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1738 0.12765407361923536 No Hit GTATTTAGCCTTGGAGGATGGTCCC 1650 0.12119057622079307 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1388 0.1019469816936126 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACTGCGC 40 2.7636113E-4 16.622782 8 CGAGCAA 35 0.0021724822 16.282942 10 CCGAATG 120 7.421477E-10 12.664045 18 GGTATCA 1640 0.0 12.49118 1 AAGCACG 70 1.09281755E-4 12.211757 18 AATCGCC 55 0.003072868 12.088407 18 TAAAACG 80 2.8707409E-5 11.872979 12 GTAGTAA 65 7.825546E-4 11.726961 1 GTATCGT 75 2.0767341E-4 11.398059 10 AGCACGA 75 2.0774434E-4 11.39764 19 TAGGACC 370 0.0 11.29579 4 GGACCTA 95 1.36175095E-5 10.998531 6 TAGAACC 70 0.0014928568 10.855693 4 CCTACAC 140 8.360075E-9 10.855693 3 GGACCGG 70 0.0014928568 10.855693 6 CGGACTG 80 3.7746746E-4 10.686073 5 GTCCTAA 700 0.0 10.480971 1 GGCGAGG 590 0.0 10.463935 19 ACGAAGG 100 2.4032528E-5 10.447837 16 TGTCCCG 500 0.0 10.258253 11 >>END_MODULE