FastQCFastQC Report
Thu 26 May 2016
SRR1512883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1361492
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77880.5720195197621433No Hit
TATCAACGCAGAGTACTTTTTTTTT52660.38678156022951293No Hit
GGTATCAACGCAGAGTACTTTTTTT44370.3258924767828235No Hit
GTACTGGTTCACTATCGGTCAGTCA33020.24252805010973258No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25720.18891040123629077No Hit
GTCCTACAGTGGACATTTCTAAATT23110.1697402555431835No Hit
GGATACCACGTGTCCCGCCCTACTC22300.16379089998325366No Hit
GTCCTAAAGTGTGTATTTCTCATTT21700.15938396993886117No Hit
CTGTAGGACGTGGAATATGGCAAGA21080.1548301422263223No Hit
CTTTAGGACGTGAAATATGGCGAGG19300.14175624976129128No Hit
ATCATTAACTGAATCCATAGGTTAA18910.13889174523243616No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17640.12956374330513878No Hit
CCCATATTCAGACAGGATACCACGT16430.12067643438228061No Hit
TATTCAGACAGGATACCACGTGTCC16020.1176650321852791No Hit
ACCCTGTATCGCGCGCCTTTCCAGA14350.10539907689505337No Hit
CTATCGGTCAGTCAGGAGTATTTAG14270.1048114862224677No Hit
GTATTTAGCCTTGGAGGATGGTCCC14160.1040035490476624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAAAT2850.013.3460374
CCCCGTA3350.013.31949315
CCGTACA3650.012.48209117
GGTATCA14950.012.408591
CTATAAG1001.4159559E-712.3672281
GCACCGT550.003046301212.1024286
CCCGTAC3800.011.74045916
GTGTAAC657.929017E-411.7086181
TAGACTG907.379873E-611.6221745
CTTACAC1501.72804E-1011.4125413
ACCCCGT752.0863049E-411.39241914
GCGCCAC1251.848457E-811.39241813
TAGGACC4050.011.2699874
GCCCCGT3150.011.15130814
AGCGTGT600.005833517311.0938946
TAATACC600.005833517311.0938944
CTTATAC1303.1972377E-810.976831
CCTAAGA1053.4192217E-610.8690882
ATTAGAC700.001477578410.8690873
CGTCTCG700.001499495210.84992112
Produced by FastQC (version 0.11.5)