##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512883_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1361492 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.148816151692408 31.0 30.0 34.0 26.0 34.0 2 30.16153822424223 31.0 30.0 34.0 26.0 34.0 3 30.34091055988577 31.0 30.0 34.0 26.0 34.0 4 33.917071124912965 37.0 35.0 37.0 30.0 37.0 5 33.47055142446669 37.0 35.0 37.0 28.0 37.0 6 33.30706386816816 37.0 35.0 37.0 27.0 37.0 7 33.14332070992705 37.0 35.0 37.0 26.0 37.0 8 33.08358403868697 37.0 35.0 37.0 26.0 37.0 9 34.37656115496823 39.0 35.0 39.0 25.0 39.0 10 34.310551953298294 39.0 35.0 39.0 25.0 39.0 11 34.28251800230923 39.0 35.0 39.0 25.0 39.0 12 34.14974013802505 39.0 35.0 39.0 25.0 39.0 13 34.13076389725389 39.0 35.0 39.0 25.0 39.0 14 35.1870550836876 40.0 35.0 41.0 25.0 41.0 15 35.17914097181621 40.0 35.0 41.0 25.0 41.0 16 35.17963381349284 40.0 35.0 41.0 25.0 41.0 17 35.18010094807755 40.0 35.0 41.0 25.0 41.0 18 35.10922576115027 40.0 35.0 41.0 25.0 41.0 19 35.118630149865 40.0 35.0 41.0 24.0 41.0 20 35.100515463917525 40.0 35.0 41.0 24.0 41.0 21 35.06515499172966 40.0 35.0 41.0 24.0 41.0 22 35.028620072684966 40.0 35.0 41.0 24.0 41.0 23 34.87433492080747 40.0 35.0 41.0 22.0 41.0 24 34.81548477699465 40.0 35.0 41.0 22.0 41.0 25 34.74419166620149 40.0 35.0 41.0 21.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.49005146924073983 1101 2 0.4180988505744665 1101 3 0.472630615315925 1101 4 0.4999867490372907 1101 5 0.4884361322420787 1101 6 0.5854886179437884 1101 7 0.7387436112522536 1101 8 0.40794414143232416 1101 9 0.661633343964148 1101 10 0.421219107760173 1101 11 0.8375379988689247 1101 12 0.7886718011784666 1101 13 0.8002644860775519 1101 14 1.1654662855889555 1101 15 0.7600587294591747 1101 16 0.9175896385752793 1101 17 1.0478163327876686 1101 18 0.9034541193235768 1101 19 0.8868413831510651 1101 20 0.8604070881723587 1101 21 1.0713312048103276 1101 22 0.8116220907748897 1101 23 0.9894503437677713 1101 24 0.9949315689855496 1101 25 1.0859827248888791 1102 1 0.6535622417708957 1102 2 0.7639789624841811 1102 3 0.48852277086985296 1102 4 0.4977875997004091 1102 5 0.5208872219837346 1102 6 0.5095856676098265 1102 7 0.461156273299558 1102 8 0.5422235970423799 1102 9 0.48601504853456134 1102 10 0.602485283781121 1102 11 0.6482977532876788 1102 12 0.5632927278356874 1102 13 0.6216166184054757 1102 14 0.5340721368765031 1102 15 0.6846316394419034 1102 16 0.6117183979076657 1102 17 0.7996110367422631 1102 18 0.2822367781984809 1102 19 0.3864085871164207 1102 20 0.5368694435302004 1102 21 0.5079491944835155 1102 22 0.3988538182411432 1102 23 0.597414652090805 1102 24 0.6428875228378672 1102 25 0.5874024107205855 1103 1 0.6549856105412033 1103 2 0.8323546533088511 1103 3 0.5603489039204774 1103 4 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2216 17 -2.4727447009195984 2216 18 -2.915580572680092 2216 19 -3.0632644094953463 2216 20 -2.371972780618144 2216 21 -2.9057324171572816 2216 22 -2.471413825495887 2216 23 -3.632921806817084 2216 24 -2.6737830836286562 2216 25 -2.8246244849797826 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 41104.0 3 0.0 4 722.0 5 5911.0 6 7715.0 7 5783.0 8 4836.0 9 3985.0 10 3237.0 11 2916.0 12 2632.0 13 2450.0 14 2396.0 15 2318.0 16 2425.0 17 2549.0 18 2678.0 19 3414.0 20 4635.0 21 6087.0 22 7541.0 23 8920.0 24 10548.0 25 11827.0 26 13120.0 27 14433.0 28 15831.0 29 18874.0 30 23945.0 31 30505.0 32 38076.0 33 49352.0 34 64091.0 35 84026.0 36 115399.0 37 179608.0 38 567603.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.770833333333336 18.8872471183251 13.106989531874852 26.234930016466713 2 14.216640825354368 20.415469515824437 39.40927494911344 25.95861470970776 3 17.744677287359632 25.536091304852484 29.05501005699207 27.66422135079582 4 12.007289431719604 16.66579747807992 36.23980203066938 35.0871110595311 5 13.338323815176844 38.50056344939754 33.87528301027432 14.285829725151295 6 32.29807411065596 35.528638472810755 18.044538120203832 14.128749296329449 7 28.787958977254018 31.701994095923602 20.967262954860864 18.54278397196152 8 26.685673975662805 34.89007113044501 20.306933748750808 18.11732114514138 9 27.778459332371973 14.036555649993717 19.48596417895975 38.699020838674556 10 16.829612581849553 28.1987918120688 32.10919984921532 22.862395756866324 11 34.77442670153222 22.25183999059805 23.58574135828767 19.387991949582055 12 24.371460679108917 24.743406121234823 29.811383117026196 21.07375008263006 13 29.60458227102417 21.37097929295934 25.487266613340033 23.537171822676452 14 22.036257388974416 22.07217397435747 26.972694581222896 28.918874055445222 15 24.597559130195883 29.534833154340134 22.99249697719416 22.875110738269825 16 22.97774754697079 27.085864469677045 26.576894881509766 23.3594931018424 17 22.195257210223037 27.729932020258037 26.68728216918178 23.38752860033714 18 22.28018436564629 25.71188276989184 29.29599500523349 22.71193785922838 19 23.821128895783385 26.11225203309359 27.58940323414657 22.47721583697645 20 24.942562674258383 25.230336897091703 27.77285667697894 22.05424375167097 21 24.387857705791234 25.307806379632886 26.936244068075467 23.368091846500413 22 24.477850704523284 26.7261261208325 26.617054281785812 22.178968892858403 23 23.30553539793109 26.17731136135945 27.60787430260332 22.909278938106137 24 24.096873136235835 25.562471171332625 27.716431679363524 22.624224013068016 25 23.358859251468242 26.078008537692472 27.69851016384966 22.864622046989627 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 120.0 2 358.5 3 597.0 4 597.0 5 597.0 6 1340.5 7 2084.0 8 2084.0 9 2084.0 10 2231.0 11 2378.0 12 2378.0 13 2378.0 14 2911.0 15 3444.0 16 3444.0 17 3444.0 18 7453.5 19 11463.0 20 11463.0 21 11463.0 22 19121.0 23 26779.0 24 26779.0 25 26779.0 26 42055.0 27 57331.0 28 57331.0 29 57331.0 30 71467.5 31 85604.0 32 85604.0 33 85604.0 34 98012.5 35 110421.0 36 110421.0 37 110421.0 38 119557.0 39 128693.0 40 128693.0 41 128693.0 42 141951.0 43 155209.0 44 155209.0 45 155209.0 46 173368.0 47 191527.0 48 191527.0 49 191527.0 50 187328.0 51 183129.0 52 183129.0 53 183129.0 54 163877.0 55 144625.0 56 144625.0 57 144625.0 58 126205.0 59 107785.0 60 107785.0 61 107785.0 62 91996.5 63 76208.0 64 76208.0 65 76208.0 66 58134.0 67 40060.0 68 40060.0 69 40060.0 70 30285.0 71 20510.0 72 20510.0 73 20510.0 74 14707.5 75 8905.0 76 8905.0 77 8905.0 78 6049.0 79 3193.0 80 3193.0 81 3193.0 82 2108.5 83 1024.0 84 1024.0 85 1024.0 86 680.0 87 336.0 88 336.0 89 336.0 90 199.5 91 63.0 92 63.0 93 63.0 94 33.5 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08608203353379969 2 0.04568517479353533 3 0.01836220851830198 4 0.005067969551051346 5 0.017187027173130653 6 0.0565556022363701 7 0.07903094546277172 8 0.04495068645280324 9 0.016525987666471783 10 0.04487723761873004 11 0.0052883160532709705 12 1.4689766814641585E-4 13 0.005214867219197763 14 2.937953362928317E-4 15 0.012780097128738179 16 0.008373167084345703 17 0.0041865835421728516 18 0.0049210718829049305 19 0.0014689766814641584 20 5.875906725856634E-4 21 2.2034650221962378E-4 22 2.937953362928317E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1361492.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.177447308484275 #Duplication Level Percentage of deduplicated Percentage of total 1 72.72244312514376 35.76304114931001 2 14.591264134685689 14.351222462953636 3 5.351079592537052 7.894573041164891 4 2.4947661290090983 4.9074491942534495 5 1.398999831353553 3.439962024548387 6 0.8438872959472387 2.490013381844676 7 0.5485813880328135 1.8884482613079197 8 0.3697678149011473 1.454738978693963 9 0.26728047293538654 1.1829754236930055 >10 1.2546793786674437 10.674911178139444 >50 0.08153455151765679 2.796448755163021 >100 0.06387159008001124 6.204065038343809 >500 0.006896911122843702 2.2549808821375286 >1k 0.0046479183653946685 3.7346577072605025 >5k 2.9986570099320447E-4 0.9625125211857337 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7788 0.5720195197621433 No Hit TATCAACGCAGAGTACTTTTTTTTT 5266 0.38678156022951293 No Hit GGTATCAACGCAGAGTACTTTTTTT 4437 0.3258924767828235 No Hit GTACTGGTTCACTATCGGTCAGTCA 3302 0.24252805010973258 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2572 0.18891040123629077 No Hit GTCCTACAGTGGACATTTCTAAATT 2311 0.1697402555431835 No Hit GGATACCACGTGTCCCGCCCTACTC 2230 0.16379089998325366 No Hit GTCCTAAAGTGTGTATTTCTCATTT 2170 0.15938396993886117 No Hit CTGTAGGACGTGGAATATGGCAAGA 2108 0.1548301422263223 No Hit CTTTAGGACGTGAAATATGGCGAGG 1930 0.14175624976129128 No Hit ATCATTAACTGAATCCATAGGTTAA 1891 0.13889174523243616 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1764 0.12956374330513878 No Hit CCCATATTCAGACAGGATACCACGT 1643 0.12067643438228061 No Hit TATTCAGACAGGATACCACGTGTCC 1602 0.1176650321852791 No Hit ACCCTGTATCGCGCGCCTTTCCAGA 1435 0.10539907689505337 No Hit CTATCGGTCAGTCAGGAGTATTTAG 1427 0.1048114862224677 No Hit GTATTTAGCCTTGGAGGATGGTCCC 1416 0.1040035490476624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGAAAT 285 0.0 13.346037 4 CCCCGTA 335 0.0 13.319493 15 CCGTACA 365 0.0 12.482091 17 GGTATCA 1495 0.0 12.40859 1 CTATAAG 100 1.4159559E-7 12.367228 1 GCACCGT 55 0.0030463012 12.102428 6 CCCGTAC 380 0.0 11.740459 16 GTGTAAC 65 7.929017E-4 11.708618 1 TAGACTG 90 7.379873E-6 11.622174 5 CTTACAC 150 1.72804E-10 11.412541 3 ACCCCGT 75 2.0863049E-4 11.392419 14 GCGCCAC 125 1.848457E-8 11.392418 13 TAGGACC 405 0.0 11.269987 4 GCCCCGT 315 0.0 11.151308 14 AGCGTGT 60 0.0058335173 11.093894 6 TAATACC 60 0.0058335173 11.093894 4 CTTATAC 130 3.1972377E-8 10.97683 1 CCTAAGA 105 3.4192217E-6 10.869088 2 ATTAGAC 70 0.0014775784 10.869087 3 CGTCTCG 70 0.0014994952 10.849921 12 >>END_MODULE