FastQCFastQC Report
Thu 26 May 2016
SRR1512882_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512882_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2328261
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT102650.4408869967757051No Hit
GTATCAACGCAGAGTACTTTTTTTT98830.4244799015230681No Hit
CTGTAGGACGTGGAATATGGCAAGA85960.36920259369546626No Hit
GTCCTAAAGTGTGTATTTCTCATTT78050.3352287393896131No Hit
CTTTAGGACGTGAAATATGGCGAGG65230.28016618411767413No Hit
GTCCTACAGTGTGCATTTCTCATTT61260.2631148311980487No Hit
GGTATCAACGCAGAGTACTTTTTTT54280.23313537442752338No Hit
TATCAACGCAGAGTACTTTTTTTTT53890.2314603044933536No Hit
CTGTAGGACCTGGAATATGGCGAGA44670.1918599332291354No Hit
CTGAAGGACCTGGAATATGGCGAGA36650.1574136233008241No Hit
GTCCTTCAGTGTGCATTTCTCATTT30160.12953874157579412No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27410.11772735101434074No Hit
GAATATGGCAAGAAAACTGAAAATC26400.1133893493899524No Hit
TTTCTAAATTTTCCACCTTTTTCAG25540.10969560543255247No Hit
ATTTAGAAATGTCCACTGTAGGACG24550.10544350483042922No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC15200.015.37264
GTCCTAT3700.012.3601281
AGGACCT26450.012.1379915
TGTAGGA49750.012.1237352
TTCGCAT550.003072330512.08928117
CTGTAGG48050.011.9367631
GGCGAGG18500.011.86031119
CCACCTT9750.011.78790213
GGACCGT658.03512E-411.6907346
ATTTCGC658.036569E-411.69048215
TCCTATA3950.011.5425012
GGTATCA22400.011.5266921
GTAGGAC49500.011.5133533
GGACCTG26100.011.5003676
TGTCGAG1850.011.29553318
ACCTTTT10100.011.28536715
GTCCTAC44800.011.2714891
CACCTTT9850.011.1860714
CATTTCG855.333738E-511.17472614
GACGTGG23050.011.1264857
Produced by FastQC (version 0.11.5)