##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512882_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2328261
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.70674121157379	31.0	30.0	33.0	22.0	34.0
2	28.885896383609914	31.0	30.0	34.0	20.0	34.0
3	29.10934212272593	31.0	30.0	34.0	22.0	34.0
4	32.4962021010531	35.0	33.0	37.0	24.0	37.0
5	32.740773908079895	35.0	33.0	37.0	26.0	37.0
6	32.632946220376496	37.0	33.0	37.0	25.0	37.0
7	32.527440437304925	37.0	33.0	37.0	23.0	37.0
8	32.49943498602605	37.0	33.0	37.0	23.0	37.0
9	33.77153506415303	39.0	34.0	39.0	22.0	39.0
10	33.62380119754615	38.0	34.0	39.0	19.0	39.0
11	33.69453510581503	38.0	34.0	39.0	20.0	39.0
12	33.59137184362063	38.0	34.0	39.0	18.0	39.0
13	33.57246717614563	38.0	34.0	39.0	18.0	39.0
14	34.44158064753049	39.0	34.0	41.0	17.0	41.0
15	34.62241561405701	39.0	34.0	41.0	18.0	41.0
16	34.57442099489705	39.0	34.0	41.0	18.0	41.0
17	34.378797308377365	39.0	34.0	41.0	17.0	41.0
18	34.36196242603385	39.0	34.0	41.0	17.0	41.0
19	34.46964107546362	39.0	34.0	41.0	18.0	41.0
20	34.44922540900698	39.0	34.0	41.0	18.0	41.0
21	34.346794453027385	39.0	34.0	41.0	17.0	41.0
22	34.373320688702854	39.0	34.0	41.0	16.0	41.0
23	34.063110192542844	39.0	34.0	41.0	10.0	41.0
24	34.131109871273026	39.0	34.0	41.0	2.0	41.0
25	33.866932873934665	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5864438240138092
1101	2	0.2855007868522428
1101	3	0.7302442578097832
1101	4	0.7934761420968925
1101	5	0.7956510754615067
1101	6	0.6348628966341536
1101	7	0.587229642357137
1101	8	0.8105856674809004
1101	9	0.8310516383122462
1101	10	0.927692432499228
1101	11	0.9498596349161872
1101	12	0.8351039668753089
1101	13	1.215994567337873
1101	14	1.1985575068275551
1101	15	1.0938617002146955
1101	16	1.0429813524108198
1101	17	0.303225648398616
1101	18	0.7911003370668723
1101	19	0.3319495163447286
1101	20	-0.1471169243801782
1101	21	0.7062746598207212
1101	22	0.8547465014746649
1101	23	0.39814355953728153
1101	24	0.8296940778654118
1101	25	0.42672042177868974
1102	1	0.8727410180647901
1102	2	0.8820771933019991
1102	3	0.8880199626773333
1102	4	1.0111963341584413
1102	5	0.9337821747107782
1102	6	0.8764471385326473
1102	7	0.8037048982151092
1102	8	0.927854115348012
1102	9	1.1026541186134793
1102	10	1.2887852326219829
1102	11	1.020397739960579
1102	12	1.1882146362269665
1102	13	1.0887450567210806
1102	14	1.3750935472330994
1102	15	1.1931782728534444
1102	16	1.1802833297696722
1102	17	1.3305144312112205
1102	18	1.3238007828860532
1102	19	1.1042862423805175
1102	20	1.4404638703766395
1102	21	1.4506683449731739
1102	22	1.360541467189357
1102	23	1.3226514606928887
1102	24	0.6957423910220513
1102	25	1.1066428345043988
1103	1	0.7453069998048711
1103	2	0.8364992460903622
1103	3	1.0237404210850016
1103	4	1.1903528735565203
1103	5	0.9858281492283254
1103	6	0.6814963698757452
1103	7	0.4702091570550948
1103	8	0.7302603617952599
1103	9	0.8981499207042916
1103	10	1.058366496846915
1103	11	0.7615269214295921
1103	12	0.9922275238341598
1103	13	1.1669945445679915
1103	14	1.1153366314015685
1103	15	1.033135948239412
1103	16	1.1698786526620424
1103	17	0.8220601341408766
1103	18	1.2208112354904372
1103	19	1.1474337937425858
1103	20	1.3133264810830738
1103	21	1.2007271580560541
1103	22	1.1289034466675645
1103	23	0.5079338489424572
1103	24	0.5970139870894897
1103	25	1.0113378498448427
1104	1	0.9858859731603928
1104	2	0.927202755407702
1104	3	0.9843471910135015
1104	4	1.0549780770018344
1104	5	0.779286079480876
1104	6	0.7152640856192107
1104	7	0.9121699126829839
1104	8	0.7545381080783855
1104	9	1.1891182289536602
1104	10	1.1183215102880197
1104	11	0.7756333164700919
1104	12	1.0918551960540483
1104	13	1.0685379858568709
1104	14	1.4162696453181454
1104	15	1.1683362847242407
1104	16	1.1096131222412566
1104	17	1.3393214402140572
1104	18	1.1036347821137014
1104	19	0.7137169164681083
1104	20	0.8817645697029377
1104	21	1.0769340230780884
1104	22	0.7613943487760793
1104	23	1.100477783986257
1104	24	1.0369210880166477
1104	25	0.6240818470651845
1105	1	0.4747330314735585
1105	2	0.5567814712202726
1105	3	0.7408189382032226
1105	4	0.7198063513218784
1105	5	0.5614328990175252
1105	6	0.7602258651097387
1105	7	0.7058728645824459
1105	8	0.7730127943340008
1105	9	0.5387436516929185
1105	10	0.7082761228681633
1105	11	0.6154626843540214
1105	12	0.7573371504424955
1105	13	0.8013834029204432
1105	14	0.8893980152572496
1105	15	0.7490320082568331
1105	16	0.7766997415382306
1105	17	1.0310033053098522
1105	18	0.9372584189998818
1105	19	0.5774139576999886
1105	20	0.8170386023507845
1105	21	0.6375728089872013
1105	22	0.6995188776850298
1105	23	0.9906982139285745
1105	24	1.005255888472874
1105	25	1.145031602775667
1106	1	0.6444853760179896
1106	2	0.5671834642473961
1106	3	0.5200390036830811
1106	4	0.6067718972037994
1106	5	0.5204357442785579
1106	6	0.6603981113235804
1106	7	0.6283087354067973
1106	8	0.4816619948790333
1106	9	0.6705951071726872
1106	10	0.3550104992324137
1106	11	0.2327925090393137
1106	12	0.4199504884955232
1106	13	0.48692680581954306
1106	14	0.7155091170937737
1106	15	0.4535971755782384
1106	16	0.5777863892581578
1106	17	0.6717626187419654
1106	18	0.556329985462412
1106	19	0.5824923327558906
1106	20	0.7386425678400883
1106	21	0.6111151611829158
1106	22	0.6465482546838146
1106	23	0.7957988676782293
1106	24	0.4212861916330084
1106	25	0.6462437823661702
1107	1	0.7863413241393467
1107	2	0.766624563573842
1107	3	0.738839920913076
1107	4	0.8112115742653145
1107	5	0.7769172295303406
1107	6	0.7930891761840968
1107	7	0.5095564485851085
1107	8	0.7255959913155152
1107	9	0.6229181108100761
1107	10	0.7979881461139939
1107	11	0.6794760431669857
1107	12	0.7835103449078389
1107	13	0.6574477910623102
1107	14	0.8234562623550161
1107	15	0.576678644891345
1107	16	0.6478122866956966
1107	17	0.8623398663422961
1107	18	0.8664817577092236
1107	19	0.7401035733606491
1107	20	0.8784814592529244
1107	21	0.7187103758576683
1107	22	0.7335786527609329
1107	23	1.057148483666694
1107	24	0.9328411489388841
1107	25	1.1501409046210398
1108	1	0.7443452608650851
1108	2	0.678039590333146
1108	3	0.6963988916313433
1108	4	0.7072794215779084
1108	5	0.6118597276251307
1108	6	0.7963500527204275
1108	7	0.7295183500699309
1108	8	0.7664031316292039
1108	9	0.801388866581668
1108	10	0.9078930071811442
1108	11	0.6293377920960168
1108	12	0.7840523487787578
1108	13	0.7582940420964732
1108	14	0.8474511745594526
1108	15	0.7314967002916006
1108	16	0.6207090955720957
1108	17	0.8262012789849322
1108	18	0.9000876707727841
1108	19	1.000754454829739
1108	20	0.9207150979859762
1108	21	0.8836032898349941
1108	22	0.8568015423198574
1108	23	1.162001589812455
1108	24	1.045262859878143
1108	25	1.1193850605296518
1109	1	0.6280336241671058
1109	2	0.5894846346023321
1109	3	0.7333404796041023
1109	4	0.7384680098877396
1109	5	0.6088162808231559
1109	6	0.7214385822635094
1109	7	0.5988813220789595
1109	8	0.5373569000246903
1109	9	0.6983799739201046
1109	10	0.7779188257242566
1109	11	0.7807339876110362
1109	12	0.5578954645104233
1109	13	0.7724306033065389
1109	14	0.8962696453181422
1109	15	0.712228902173905
1109	16	0.4626332564694451
1109	17	0.9089858697442637
1109	18	0.8985341109727614
1109	19	0.8162672520385783
1109	20	0.747536381783469
1109	21	0.7510279828096316
1109	22	0.6827366306552776
1109	23	1.0666522806305494
1109	24	0.853296926942825
1109	25	0.36985365914572554
1110	1	0.49615545769720626
1110	2	0.5748702126947016
1110	3	0.4497558140932334
1110	4	0.5356755955885006
1110	5	0.43748339420803717
1110	6	0.4999856844449795
1110	7	0.2786481068068696
1110	8	0.3720238645035039
1110	9	0.5299196272525819
1110	10	0.42465647095199444
1110	11	0.47746301755888254
1110	12	0.4425330377668928
1110	13	0.5817806992091406
1110	14	0.4092863759789651
1110	15	0.49707997837481344
1110	16	0.5607169771358329
1110	17	0.6455130965104843
1110	18	0.06568975681739886
1110	19	0.4517796484690919
1110	20	0.5395383530162405
1110	21	0.6744916798637774
1110	22	0.6942029654741262
1110	23	0.9308903601676661
1110	24	0.7275564560451002
1110	25	1.0142369575570385
1111	1	0.11612240637191462
1111	2	0.09805697555843196
1111	3	0.1615619830243915
1111	4	-0.11681462817515609
1111	5	0.07221626180660223
1111	6	-0.07176009231834968
1111	7	-0.054066943916026844
1111	8	0.1519019109592108
1111	9	0.13809472556566504
1111	10	0.0872241293702345
1111	11	-0.03539277029922516
1111	12	-0.39212751681926505
1111	13	-0.007753570253633768
1111	14	-0.04081870452909442
1111	15	-0.030317630085171743
1111	16	0.12016551734072323
1111	17	-0.27814795837835504
1111	18	-0.031086616356716945
1111	19	-0.05975082445462476
1111	20	0.14642732182929308
1111	21	0.039724074907177
1111	22	-0.2200745546682441
1111	23	-0.05265006289099716
1111	24	0.018235490905730956
1111	25	0.11190633500851277
1112	1	0.28845510355760595
1112	2	0.24147141341386558
1112	3	0.1833292459145568
1112	4	0.3611404038157886
1112	5	0.28531403408524625
1112	6	0.1350640395322813
1112	7	-0.07260872602427781
1112	8	0.10119874983890753
1112	9	0.13150293961414405
1112	10	0.1282127739340737
1112	11	0.04915219770983015
1112	12	0.04786817240569263
1112	13	0.044191484143013327
1112	14	0.144694696445832
1112	15	-0.15684633474172927
1112	16	0.16675551645734288
1112	17	0.43748300371321136
1112	18	0.12420582799900615
1112	19	0.252483875018676
1112	20	-0.017086857551724677
1112	21	0.03182593482161877
1112	22	-0.1284431227796432
1112	23	0.21331385853186902
1112	24	0.24283396931399892
1112	25	0.2406801274465593
1113	1	-0.05884117434407443
1113	2	0.0216162397721682
1113	3	-0.03558650084781689
1113	4	0.13933375239235346
1113	5	-0.07249749954670648
1113	6	-0.2863614529054459
1113	7	-0.31258075406183394
1113	8	-0.15861720683032132
1113	9	-0.37755046481264287
1113	10	-0.2869143415334392
1113	11	-0.3540411508667347
1113	12	-0.47956028121801353
1113	13	-0.45988366063648556
1113	14	-0.11854513150551327
1113	15	-0.40907190964286855
1113	16	-0.5347316361186998
1113	17	-0.2652750692669059
1113	18	-0.49634887897477853
1113	19	-0.7825455025700165
1113	20	-0.48950495639536484
1113	21	-0.3306405644193475
1113	22	-0.8244717600718445
1113	23	-4.311624293912985
1113	24	-2.0407932960869815
1113	25	-1.1357782097912974
1114	1	-0.4728145345320911
1114	2	-0.6747725808373808
1114	3	-0.5912453289067798
1114	4	-0.40373114280164657
1114	5	-0.562830346703258
1114	6	-1.0788635660813846
1114	7	-0.9716221685478139
1114	8	-0.8061800628408982
1114	9	-1.0446630199621438
1114	10	-0.6760050550971428
1114	11	-0.9641568989811802
1114	12	-1.6962913987055792
1114	13	-0.9145566639046976
1114	14	-2.733373042483745
1114	15	-1.455229127424147
1114	16	-1.4771687550259145
1114	17	-1.2840705777961077
1114	18	-1.330827476327599
1114	19	-1.2263695107568537
1114	20	-0.9087673702066255
1114	21	-0.7501196257929976
1114	22	-0.9754116217183864
1114	23	-5.132688143178957
1114	24	-2.217846556899296
1114	25	-2.215938121401596
1115	1	-1.3774655793720498
1115	2	-1.1935613185461413
1115	3	-1.0297193644254605
1115	4	-1.0033330485370051
1115	5	-1.0221578927071668
1115	6	-1.1965661134582497
1115	7	-1.6098533998391922
1115	8	-1.8994080345598263
1115	9	-2.9570688751751035
1115	10	-3.8173564628512473
1115	11	-2.563666250949751
1115	12	-2.9369006641614135
1115	13	-2.288785301752309
1115	14	-2.7997119643631976
1115	15	-2.520784815071174
1115	16	-2.615877776966812
1115	17	-2.381237818737631
1115	18	-4.861252467718323
1115	19	-3.0177626888496825
1115	20	-3.3806873972911227
1115	21	-3.0160038227784263
1115	22	-2.6008964387009854
1115	23	-2.9682640343602777
1115	24	-2.679586638785956
1115	25	-2.9123493289035878
1116	1	-1.205225814772124
1116	2	-1.099580227851849
1116	3	-1.5318348110967328
1116	4	-1.3138840290971743
1116	5	-1.445048599035001
1116	6	-3.603602045149934
1116	7	-2.1832326087184697
1116	8	-2.6598928361962493
1116	9	-3.5463251917984095
1116	10	-2.856909581920437
1116	11	-2.4655518944987627
1116	12	-2.337262095886821
1116	13	-3.6464479277976345
1116	14	-6.696631271653999
1116	15	-4.518998622218859
1116	16	-3.326387008288588
1116	17	-4.393216386246515
1116	18	-3.076686103748827
1116	19	-2.9330536664564093
1116	20	-3.4942919062088684
1116	21	-4.269225897733808
1116	22	-3.780904934397533
1116	23	-2.781515406706969
1116	24	-3.7863810994459612
1116	25	-3.730555551910154
1201	1	0.5659310862765317
1201	2	0.5960815944672149
1201	3	1.0013343904445868
1201	4	0.8645173898601826
1201	5	0.5489978444482091
1201	6	0.9777330842413718
1201	7	1.1067735987727403
1201	8	1.0359547636334483
1201	9	1.532916027374327
1201	10	1.327359615685296
1201	11	1.554743202835418
1201	12	1.411607407263432
1201	13	1.485585410186843
1201	14	1.4468594493094287
1201	15	1.3363238989885744
1201	16	1.412987716667672
1201	17	1.6558614345841818
1201	18	1.4858482878991452
1201	19	1.7231130377772033
1201	20	1.6251172372288991
1201	21	1.7720644733203201
1201	22	1.4566411745609287
1201	23	1.8525986930636975
1201	24	1.7143525294140431
1201	25	1.840277826145737
1202	1	0.807857142329798
1202	2	0.8626399076574351
1202	3	0.8629357005706559
1202	4	1.0673773375629665
1202	5	0.9099157863461826
1202	6	1.0368770094078386
1202	7	0.9913368601300121
1202	8	1.0545801795999736
1202	9	1.31590176095807
1202	10	1.182185351545428
1202	11	1.1692890767170567
1202	12	1.3641613893098565
1202	13	1.3240377900694895
1202	14	1.7029504572438725
1202	15	1.4902930657975162
1202	16	1.2290998861034552
1202	17	1.5310127700201832
1202	18	1.4429785756536333
1202	19	1.5480064814558645
1202	20	1.3108667707173893
1202	21	1.51804828095284
1202	22	1.5074426308556141
1202	23	1.6887079570987282
1202	24	1.6642635702364217
1202	25	1.9097321799037417
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2211	12	-0.4140237471993302
2211	13	-0.3962748950216195
2211	14	-0.15129186361222935
2211	15	-0.6139646695860534
2211	16	-0.5045908151435654
2211	17	-0.23921092186564863
2211	18	-0.4939740264493935
2211	19	-0.3898526069682191
2211	20	-0.6181616805766268
2211	21	-0.31044157740913647
2211	22	-0.3967324452921517
2211	23	-0.3528749448458228
2211	24	-0.6149161302695845
2211	25	-0.22130221523207183
2212	1	-0.264336317185613
2212	2	-0.23546691843750978
2212	3	-0.5420113128421846
2212	4	-0.2763195716327118
2212	5	-0.5434612554801319
2212	6	-0.58532216821731
2212	7	-0.46747718177672937
2212	8	-0.5221966215492593
2212	9	-0.3990528298724456
2212	10	-0.6624567796346525
2212	11	-0.8965512308655974
2212	12	-0.29665030190041364
2212	13	-0.25374798076490634
2212	14	-0.35532032734718655
2212	15	-0.5661029166216949
2212	16	-0.6756199523433395
2212	17	-0.38085950142696845
2212	18	-0.08436380188474857
2212	19	-0.4155735864679002
2212	20	-0.3079081076713308
2212	21	-0.16797636373119929
2212	22	-0.1641301739620573
2212	23	-0.20092566332060358
2212	24	-0.12034113868227791
2212	25	0.03971676683118375
2213	1	-0.11472334776683368
2213	2	0.01545089737081895
2213	3	-0.2018481475941023
2213	4	0.04577120472666252
2213	5	0.04306612803782173
2213	6	0.2192226097407186
2213	7	-0.05382937772432683
2213	8	0.036363210198430806
2213	9	-0.34031645601133675
2213	10	-0.0470958398105239
2213	11	-0.05737522650577631
2213	12	0.025997266913009298
2213	13	-0.15126633745733642
2213	14	-0.36818299308493607
2213	15	-0.1695684849646355
2213	16	-0.1074739440387873
2213	17	-0.18692513024273438
2213	18	-0.08901695833883849
2213	19	-0.0624248683246833
2213	20	0.005284223748631689
2213	21	-0.12859686185086616
2213	22	-0.3289111661708759
2213	23	-0.048993866671231956
2213	24	0.15934559162683826
2213	25	0.45506444718370176
2214	1	-2.586614361073142
2214	2	-1.3234366428955404
2214	3	-1.5054911927477157
2214	4	-3.225209322966844
2214	5	-1.6955419716893232
2214	6	-1.3256202008208362
2214	7	-1.2106952517790148
2214	8	-1.2108417149115347
2214	9	-0.9639222266734961
2214	10	-0.9865492081803922
2214	11	-1.0072485023715103
2214	12	-0.8747028860948234
2214	13	-1.2239916081353286
2214	14	-1.19727357394288
2214	15	-1.6135599462674222
2214	16	-1.3901653286722535
2214	17	-3.250193940258299
2214	18	-3.1342090105010705
2214	19	-1.9050840445965491
2214	20	-1.7005989770687506
2214	21	-1.5913089016754398
2214	22	-2.5083577072376393
2214	23	-1.472540516732252
2214	24	-2.5757444148806776
2214	25	-2.5432621231708374
2215	1	-1.5086184768790183
2215	2	-3.2589243710836193
2215	3	-1.9916796852348462
2215	4	-3.630405284518911
2215	5	-2.170397558301989
2215	6	-2.104774636993721
2215	7	-2.1377840946482927
2215	8	-1.6360110489777782
2215	9	-3.765044987625128
2215	10	-2.3298726078542167
2215	11	-1.8439229805461217
2215	12	-1.8709492088344923
2215	13	-1.9121978968426916
2215	14	-1.4928318523133512
2215	15	-1.6024794920620877
2215	16	-1.7003284877317313
2215	17	-2.1722092509004014
2215	18	-1.6854646492914043
2215	19	-1.8354601132166763
2215	20	-1.6423486748081757
2215	21	-2.1573532436105154
2215	22	-2.2894267848934575
2215	23	-2.020643105562467
2215	24	-1.9312069108813645
2215	25	-2.8834448034287945
2216	1	-3.040775962401966
2216	2	-3.0589328110068656
2216	3	-4.477900218706893
2216	4	-4.90543069041032
2216	5	-4.7511237017393455
2216	6	-3.7692177570783265
2216	7	-5.495511102747173
2216	8	-3.3422002686121424
2216	9	-4.124198342752987
2216	10	-2.9336393972729624
2216	11	-3.2256286718102842
2216	12	-3.6028876008396438
2216	13	-3.1456654651457825
2216	14	-4.049487162403018
2216	15	-3.427883833283019
2216	16	-3.716685058987075
2216	17	-3.4515867290513356
2216	18	-2.935829116181072
2216	19	-3.8122787444333675
2216	20	-4.392251529569151
2216	21	-4.180807618074407
2216	22	-3.7837870214869014
2216	23	-3.8907556149066664
2216	24	-4.199829859893892
2216	25	-4.247875005263531
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	118842.0
3	0.0
4	1020.0
5	6608.0
6	7980.0
7	7133.0
8	6229.0
9	5887.0
10	5148.0
11	4894.0
12	4464.0
13	4393.0
14	4360.0
15	4488.0
16	4660.0
17	5038.0
18	5528.0
19	6721.0
20	8761.0
21	11303.0
22	14083.0
23	17193.0
24	19751.0
25	22451.0
26	25017.0
27	27974.0
28	30682.0
29	36302.0
30	43522.0
31	54065.0
32	68060.0
33	86632.0
34	111685.0
35	147301.0
36	203321.0
37	322735.0
38	874030.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.0530134231601	19.082729177638583	12.664255502909516	24.200001896291802
2	13.982468220589253	20.100678650911235	44.09791920192474	21.818933926574775
3	19.604676256520573	25.85398897602078	28.69224266499887	25.849092102459775
4	11.720827978199162	16.549813057134415	39.129306730630454	32.60005223403598
5	12.112820050215747	39.71343947799678	35.576374972883926	12.597365498903542
6	30.774574176562247	37.398828649723285	18.867379506743323	12.959217666971146
7	28.146743954624487	31.963341951929298	21.86634513500676	18.023568958439455
8	24.55294011927903	38.686786994793636	20.523457019434545	16.23681586649279
9	27.68140014942401	14.87142914868721	20.34032221594607	37.106848485942706
10	15.626953358422302	29.36548469942677	34.31410734260714	20.693454599543788
11	33.94931171541102	22.2690220778964	25.762073889893383	18.019592316799198
12	24.737208220513796	24.85143934658248	31.95436257999833	18.45698985290539
13	29.628782629179828	22.995307553823853	25.769035013661224	21.606874803335092
14	20.225881252423502	23.952939112999953	27.82508475275753	27.996094881819015
15	22.104741422112824	33.98978524599537	23.146421074668467	20.759052257223342
16	20.459233109766632	28.614932513006046	30.382645538309696	20.54318883891763
17	20.325959658321914	30.136220663372793	28.833904000632867	20.703915677672434
18	20.04642531921169	27.341537805378252	32.91528475600266	19.6967521194074
19	23.201140147419512	26.77373103784799	28.44384064591157	21.581288168820922
20	23.688441778981538	27.536166462457818	29.579587713075817	19.195804045484834
21	22.19515529167806	27.064795566582927	27.770842682815694	22.96920645892332
22	22.734081495246098	29.935834983606192	27.868228686113373	19.46185483503434
23	20.773927776542962	29.29905061454469	29.43180894837601	20.49521266053634
24	22.73643935730023	26.96643559567951	30.436369498629695	19.860755548390564
25	21.526056697385666	28.34568355545391	29.88540237707205	20.242857370088373
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	406.0
1	406.0
2	828.0
3	1250.0
4	1250.0
5	1250.0
6	2304.0
7	3358.0
8	3358.0
9	3358.0
10	4310.0
11	5262.0
12	5262.0
13	5262.0
14	7549.5
15	9837.0
16	9837.0
17	9837.0
18	18050.5
19	26264.0
20	26264.0
21	26264.0
22	44282.0
23	62300.0
24	62300.0
25	62300.0
26	94584.0
27	126868.0
28	126868.0
29	126868.0
30	167894.0
31	208920.0
32	208920.0
33	208920.0
34	231657.0
35	254394.0
36	254394.0
37	254394.0
38	267826.5
39	281259.0
40	281259.0
41	281259.0
42	290764.0
43	300269.0
44	300269.0
45	300269.0
46	308344.5
47	316420.0
48	316420.0
49	316420.0
50	294970.5
51	273521.0
52	273521.0
53	273521.0
54	230166.0
55	186811.0
56	186811.0
57	186811.0
58	156684.5
59	126558.0
60	126558.0
61	126558.0
62	101772.5
63	76987.0
64	76987.0
65	76987.0
66	57959.5
67	38932.0
68	38932.0
69	38932.0
70	28319.5
71	17707.0
72	17707.0
73	17707.0
74	12426.0
75	7145.0
76	7145.0
77	7145.0
78	4835.5
79	2526.0
80	2526.0
81	2526.0
82	1714.5
83	903.0
84	903.0
85	903.0
86	580.5
87	258.0
88	258.0
89	258.0
90	165.5
91	73.0
92	73.0
93	73.0
94	48.0
95	23.0
96	23.0
97	23.0
98	16.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34115590992590605
2	0.01116713289446501
3	0.010522875227476644
4	0.011897291583718493
5	0.013142856406562667
6	0.08100466399600388
7	0.08143416910732947
8	0.10540055431929668
9	0.08568626970945267
10	0.091871143312541
11	0.08774789424381546
12	0.09771241282656884
13	0.08456955642000617
14	0.08946591468911776
15	0.0866741314655015
16	0.08740429015475498
17	0.10003174042772696
18	0.10200746393982461
19	0.09646684800372467
20	0.09496358011408515
21	0.08285153597470386
22	0.10235106802888506
23	0.09577963982560374
24	0.09174229177914332
25	0.09114098462328751
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2328261.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.81406600152322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16686025829222	45.19144018104749
2	12.962901596288292	14.988760969181241
3	4.1564906954538055	7.2091088220505055
4	1.757558764305081	4.064464736043582
5	0.910380736669098	2.6316405998151287
6	0.517338852876837	1.7945677551224262
7	0.33532949152159774	1.357073294856081
8	0.2270463674541046	1.0501178938718156
9	0.15736964936665945	0.8188361365608555
>10	0.7168698830284487	7.35390884164943
>50	0.04802036055824707	1.9006362911307455
>100	0.0356716065878005	4.183615735149917
>500	0.0044926995952683565	1.7929504268759957
>1k	0.0030700113900995617	3.064816654309221
>5k	5.241482861145593E-4	2.1536877064265965
>10k+	7.487832658779419E-5	0.4443739559089248
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	10265	0.4408869967757051	No Hit
GTATCAACGCAGAGTACTTTTTTTT	9883	0.4244799015230681	No Hit
CTGTAGGACGTGGAATATGGCAAGA	8596	0.36920259369546626	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	7805	0.3352287393896131	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6523	0.28016618411767413	No Hit
GTCCTACAGTGTGCATTTCTCATTT	6126	0.2631148311980487	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5428	0.23313537442752338	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5389	0.2314603044933536	No Hit
CTGTAGGACCTGGAATATGGCGAGA	4467	0.1918599332291354	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3665	0.1574136233008241	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	3016	0.12953874157579412	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2741	0.11772735101434074	No Hit
GAATATGGCAAGAAAACTGAAAATC	2640	0.1133893493899524	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2554	0.10969560543255247	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2455	0.10544350483042922	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1520	0.0	15.3726	4
GTCCTAT	370	0.0	12.360128	1
AGGACCT	2645	0.0	12.137991	5
TGTAGGA	4975	0.0	12.123735	2
TTCGCAT	55	0.0030723305	12.089281	17
CTGTAGG	4805	0.0	11.936763	1
GGCGAGG	1850	0.0	11.860311	19
CCACCTT	975	0.0	11.787902	13
GGACCGT	65	8.03512E-4	11.690734	6
ATTTCGC	65	8.036569E-4	11.690482	15
TCCTATA	395	0.0	11.542501	2
GGTATCA	2240	0.0	11.526692	1
GTAGGAC	4950	0.0	11.513353	3
GGACCTG	2610	0.0	11.500367	6
TGTCGAG	185	0.0	11.295533	18
ACCTTTT	1010	0.0	11.285367	15
GTCCTAC	4480	0.0	11.271489	1
CACCTTT	985	0.0	11.18607	14
CATTTCG	85	5.333738E-5	11.174726	14
GACGTGG	2305	0.0	11.126485	7
>>END_MODULE