FastQCFastQC Report
Thu 26 May 2016
SRR1512882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2328261
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT125330.5382987560243461No Hit
GTCCTACAGTGGACATTTCTAAATT97130.41717831463053323No Hit
TATCAACGCAGAGTACTTTTTTTTT84440.3626741160033175No Hit
CTGTAGGACGTGGAATATGGCAAGA83750.359710530735171No Hit
GTCCTAAAGTGTGTATTTCTCATTT72660.31207841388916446No Hit
GGTATCAACGCAGAGTACTTTTTTT70960.30477682699662967No Hit
CTTTAGGACGTGAAATATGGCGAGG69250.2974322895929623No Hit
GTCCTACAGTGTGCATTTCTCATTT59550.2557702937943813No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44420.19078617045082144No Hit
CTGTAGGACCTGGAATATGGCGAGA44030.1891111005166517No Hit
CTGAAGGACCTGGAATATGGCGAGA36280.15582445438891945No Hit
TTTCTAAATTTTCCACCTTTTTCAG28410.12202240212759652No Hit
GTCCTTCAGTGTGCATTTCTCATTT28050.12047618372682445No Hit
GAATATGGCAAGAAAACTGAAAATC25160.10806348600951525No Hit
ATTTAGAAATGTCCACTGTAGGACG24710.10613071300855016No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT602.5439931E-514.2649916
GTCGAGG1500.013.9222319
TAGGACC15200.013.3265044
TCGAACT1650.012.65657319
TCCAACG2950.012.55039218
GGTATCA23300.012.5338411
AAATGTC8800.011.988087
ACGGTGC658.036338E-411.6905238
TAGAAAT10250.011.6903354
CGGTTTC1950.011.68399313
CTCGAAC1800.011.60285618
AATGTCC9600.011.4774148
CACCTTT8750.011.392138514
TGTCGAG2000.011.39189318
TATGTCG2100.011.30148216
GCCGGTT1950.011.19740111
CCGGTTT1950.011.19716112
GGCGAGG18500.011.08305319
AGGACCT27750.010.9322095
CTAGGAC4450.010.901443
Produced by FastQC (version 0.11.5)