##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512882_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2328261 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.35020386460109 31.0 31.0 34.0 26.0 34.0 2 30.387457849442136 31.0 30.0 34.0 26.0 34.0 3 30.554087363916675 31.0 31.0 34.0 27.0 34.0 4 34.1123142980963 37.0 35.0 37.0 30.0 37.0 5 33.72586192011978 37.0 35.0 37.0 28.0 37.0 6 33.599307380057475 37.0 35.0 37.0 28.0 37.0 7 33.43685609130591 37.0 35.0 37.0 27.0 37.0 8 33.37726354562483 37.0 35.0 37.0 27.0 37.0 9 34.70693964293522 39.0 35.0 39.0 27.0 39.0 10 34.62187143108097 39.0 35.0 39.0 27.0 39.0 11 34.601507734742796 39.0 35.0 39.0 27.0 39.0 12 34.47934660246424 39.0 35.0 39.0 26.0 39.0 13 34.46828942287828 39.0 35.0 39.0 27.0 39.0 14 35.56343339513912 40.0 36.0 41.0 26.0 41.0 15 35.57809841766022 40.0 36.0 41.0 27.0 41.0 16 35.58092155475696 40.0 36.0 41.0 27.0 41.0 17 35.587489976424465 40.0 36.0 41.0 27.0 41.0 18 35.522247720509 40.0 36.0 41.0 26.0 41.0 19 35.5454607537557 40.0 36.0 41.0 26.0 41.0 20 35.52742239809025 40.0 36.0 41.0 26.0 41.0 21 35.49434406194151 40.0 36.0 41.0 26.0 41.0 22 35.46293864820138 40.0 36.0 41.0 26.0 41.0 23 35.31653839496517 40.0 36.0 41.0 25.0 41.0 24 35.29034889129698 40.0 36.0 41.0 25.0 41.0 25 35.23529879167327 40.0 36.0 41.0 25.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6523754403559501 1101 2 0.49229141315197467 1101 3 0.48093187845595864 1101 4 0.5422867823624316 1101 5 0.6181507971922997 1101 6 0.6681258080124124 1101 7 0.7197594811522237 1101 8 0.5218720341754306 1101 9 0.7606366481819506 1101 10 0.45664450922915023 1101 11 0.7748641031127974 1101 12 0.8371543366411984 1101 13 0.980666912777103 1101 14 0.9751763746364972 1101 15 0.6231316394234341 1101 16 0.8743024480682493 1101 17 0.9711795476977514 1101 18 0.8724874116750243 1101 19 0.6779515337944133 1101 20 0.7653027300191937 1101 21 0.852824318841094 1101 22 0.8613604365804903 1101 23 0.9716171818531407 1101 24 1.0081749836390301 1101 25 1.0277550755190603 1102 1 0.8304640618259143 1102 2 0.8499609235712597 1102 3 0.5664025333141289 1102 4 0.6212742585579392 1102 5 0.7969242133340089 1102 6 0.729808298765974 1102 7 0.8018691348960303 1102 8 0.882414036054719 1102 9 1.0708715749130988 1102 10 0.9574475762585095 1102 11 0.9106433523529915 1102 12 0.9044377869428786 1102 13 1.018848602514602 1102 14 0.9563609668703208 1102 15 1.0844468553863251 1102 16 0.9804996309528633 1102 17 1.0665036496596159 1102 18 0.6514526310941804 1102 19 0.8497305490725893 1102 20 1.014670310935486 1102 21 0.952153577082747 1102 22 0.7385739033734566 1102 23 0.9548154426341995 1102 24 0.9650943723297019 1102 25 1.021452761649897 1103 1 0.7750162910386642 1103 2 0.9206254928313733 1103 3 0.7390785620229359 1103 4 0.584990933311559 1103 5 0.6913309145330828 1103 6 0.7159342070621477 1103 7 0.8518118822360705 1103 8 0.9650391726846124 1103 9 0.9893160710413866 1103 10 0.8193770466286807 1103 11 0.9094166370209464 1103 12 1.0643537173004631 1103 13 1.013017599288986 1103 14 1.2507534013455626 1103 15 1.2264135381278294 1103 16 1.2495280596292773 1103 17 1.1915881911441986 1103 18 1.229248927541839 1103 19 1.323261318011852 1103 20 1.1434034184285622 1103 21 1.1956681250542331 1103 22 1.1817409971749342 1103 23 1.2265449633837378 1103 24 1.238748860514697 1103 25 1.1948245160631075 1104 1 0.7449506399222301 1104 2 0.8408924862296878 1104 3 0.76549152400381 1104 4 0.5939571568179502 1104 5 0.6759765996637412 1104 6 0.6998092687815145 1104 7 0.6741566694642742 1104 8 0.7391108220088967 1104 9 0.766003158436007 1104 10 0.8908083872647126 1104 11 0.8821763612496838 1104 12 0.9620400183446591 1104 13 0.8029929115432495 1104 14 0.9578261661902161 1104 15 1.1109370646351522 1104 16 1.0376263717189573 1104 17 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-3.352412266288063 2216 20 -2.688837252644973 2216 21 -3.3249184880494838 2216 22 -2.7857055153336034 2216 23 -4.003321595717988 2216 24 -3.239172885988758 2216 25 -3.498077844054446 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 62842.0 3 4.0 4 996.0 5 9304.0 6 11631.0 7 9056.0 8 7208.0 9 6183.0 10 5142.0 11 4634.0 12 4105.0 13 3792.0 14 3656.0 15 3581.0 16 3637.0 17 3775.0 18 4298.0 19 5363.0 20 7230.0 21 9545.0 22 11767.0 23 14475.0 24 16880.0 25 19040.0 26 21054.0 27 22840.0 28 25770.0 29 30692.0 30 38806.0 31 48898.0 32 62416.0 33 80114.0 34 105980.0 35 139610.0 36 192833.0 37 305702.0 38 1025402.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.33540907750467 19.785670745075517 13.684263921858248 26.194656255561565 2 12.541616177241515 20.634490937685307 42.890315483710175 23.933577401363 3 17.780691346271983 25.766322451269563 29.517494155440787 26.93549204701766 4 10.958613440956514 17.225120837687697 38.23625298147907 33.580012739876715 5 11.68449893934278 39.883976386307765 35.65556692536565 12.775957748983805 6 30.797238320558524 37.972834460395944 18.29567504496432 12.934252174081212 7 27.739227431283165 32.099793887108596 21.862108541326815 18.298870140281423 8 24.501305370822006 39.01634130880884 20.120029652868055 16.3623236675011 9 26.953581298995 14.774316363078643 20.28942358874424 37.98267874918212 10 15.106196644919981 29.895389021003844 34.15021627795649 20.84819805611968 11 33.6193295961246 22.341426705421842 25.692415207298254 18.346828491155307 12 24.268443219683075 25.119724909739226 32.01287316590029 18.59895870467741 13 29.316959606893118 22.848006116523205 26.001022284074704 21.834011992508977 14 19.96120668179686 23.836501110712145 27.899018827988225 28.303273379502762 15 21.88010917910339 33.91588535335007 23.24050933065512 20.96349613689142 16 20.331198054323114 28.711062085460128 30.131145048201635 20.826594812015124 17 20.045787624455574 30.147671531780734 29.009982217564236 20.796558626199456 18 19.829339500225718 27.382265001411003 33.045090675739466 19.743304822623813 19 22.964264867518562 26.681149877609993 28.738392921953448 21.616192332917993 20 23.386389465627477 27.46915462674156 29.770692364057442 19.373763543573517 21 21.74851422806218 26.907708833798416 28.292997619242943 23.05077931889646 22 22.473488671749166 29.620573392032647 28.283517663481156 19.622420272737035 23 20.45715665039272 29.02694328513856 29.8463531365255 20.669546927943216 24 22.540041687766106 26.93486683838281 30.66477512615639 19.860316347694695 25 21.19777052295299 28.22082938367252 30.20849484528998 20.372905248084514 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 155.0 1 155.0 2 478.5 3 802.0 4 802.0 5 802.0 6 1994.0 7 3186.0 8 3186.0 9 3186.0 10 4263.5 11 5341.0 12 5341.0 13 5341.0 14 7521.0 15 9701.0 16 9701.0 17 9701.0 18 18377.0 19 27053.0 20 27053.0 21 27053.0 22 45726.5 23 64400.0 24 64400.0 25 64400.0 26 97821.0 27 131242.0 28 131242.0 29 131242.0 30 171220.0 31 211198.0 32 211198.0 33 211198.0 34 233128.5 35 255059.0 36 255059.0 37 255059.0 38 268080.0 39 281101.0 40 281101.0 41 281101.0 42 290777.0 43 300453.0 44 300453.0 45 300453.0 46 308450.5 47 316448.0 48 316448.0 49 316448.0 50 294169.5 51 271891.0 52 271891.0 53 271891.0 54 228561.0 55 185231.0 56 185231.0 57 185231.0 58 154847.0 59 124463.0 60 124463.0 61 124463.0 62 100173.5 63 75884.0 64 75884.0 65 75884.0 66 56729.0 67 37574.0 68 37574.0 69 37574.0 70 27324.5 71 17075.0 72 17075.0 73 17075.0 74 11833.0 75 6591.0 76 6591.0 77 6591.0 78 4472.0 79 2353.0 80 2353.0 81 2353.0 82 1570.0 83 787.0 84 787.0 85 787.0 86 499.0 87 211.0 88 211.0 89 211.0 90 134.5 91 58.0 92 58.0 93 58.0 94 31.0 95 4.0 96 4.0 97 4.0 98 2.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08706068606569452 2 0.0447114820889926 3 0.019542482565313768 4 0.005927170536292967 5 0.019714284609844 6 0.06081792376370175 7 0.08014565377335273 8 0.05738188287309714 9 0.02577030667953464 10 0.049951444447164636 11 0.006098972580823198 12 3.006535779279041E-4 13 0.006056022069690641 14 0.0010737627783139433 15 0.014732025318467304 16 0.009792716538223164 17 0.005197011847039486 18 0.005583566447232505 19 0.0020616245343627712 20 0.0015032678896395207 21 4.2950511132557734E-4 22 4.724556224581351E-4 23 0.0 24 0.0 25 8.590102226511547E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2328261.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.59594942237226 #Duplication Level Percentage of deduplicated Percentage of total 1 77.22628555948903 43.706949516025176 2 13.419345482932169 15.189611964667371 3 4.406832256702369 7.482265665396176 4 1.8694165201067772 4.232056112852413 5 0.9401659132178303 2.6604791236557377 6 0.5538960446351464 1.8808963516453676 7 0.33951420014479666 1.345058994970046 8 0.23436143109405766 1.0611126160603255 9 0.162569877318537 0.8280716898889064 >10 0.7526081594814175 7.477279931859737 >50 0.05027513594494534 1.9603145820550523 >100 0.03626193823504741 4.2379928363149295 >500 0.004500732983503289 1.6882539346228547 >1k 0.0033564788351544183 3.3869558400967987 >5k 5.339852692291119E-4 2.321608200705739 >10k+ 7.628360988987314E-5 0.5410926391833419 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12533 0.5382987560243461 No Hit GTCCTACAGTGGACATTTCTAAATT 9713 0.41717831463053323 No Hit TATCAACGCAGAGTACTTTTTTTTT 8444 0.3626741160033175 No Hit CTGTAGGACGTGGAATATGGCAAGA 8375 0.359710530735171 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7266 0.31207841388916446 No Hit GGTATCAACGCAGAGTACTTTTTTT 7096 0.30477682699662967 No Hit CTTTAGGACGTGAAATATGGCGAGG 6925 0.2974322895929623 No Hit GTCCTACAGTGTGCATTTCTCATTT 5955 0.2557702937943813 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4442 0.19078617045082144 No Hit CTGTAGGACCTGGAATATGGCGAGA 4403 0.1891111005166517 No Hit CTGAAGGACCTGGAATATGGCGAGA 3628 0.15582445438891945 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2841 0.12202240212759652 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2805 0.12047618372682445 No Hit GAATATGGCAAGAAAACTGAAAATC 2516 0.10806348600951525 No Hit ATTTAGAAATGTCCACTGTAGGACG 2471 0.10613071300855016 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGACCGT 60 2.5439931E-5 14.264991 6 GTCGAGG 150 0.0 13.92223 19 TAGGACC 1520 0.0 13.326504 4 TCGAACT 165 0.0 12.656573 19 TCCAACG 295 0.0 12.550392 18 GGTATCA 2330 0.0 12.533841 1 AAATGTC 880 0.0 11.98808 7 ACGGTGC 65 8.036338E-4 11.690523 8 TAGAAAT 1025 0.0 11.690335 4 CGGTTTC 195 0.0 11.683993 13 CTCGAAC 180 0.0 11.602856 18 AATGTCC 960 0.0 11.477414 8 CACCTTT 875 0.0 11.3921385 14 TGTCGAG 200 0.0 11.391893 18 TATGTCG 210 0.0 11.301482 16 GCCGGTT 195 0.0 11.197401 11 CCGGTTT 195 0.0 11.197161 12 GGCGAGG 1850 0.0 11.083053 19 AGGACCT 2775 0.0 10.932209 5 CTAGGAC 445 0.0 10.90144 3 >>END_MODULE