FastQCFastQC Report
Thu 26 May 2016
SRR1512881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327579
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54820.23552369221409886No Hit
GTCCTACAGTGGACATTTCTAAATT47260.20304359164608374No Hit
GTCCTAAAGTGTGTATTTCTCATTT45640.1960835700957948No Hit
CTGTAGGACGTGGAATATGGCAAGA39980.17176645776577293No Hit
CTTTAGGACGTGAAATATGGCGAGG35970.1545382562740083No Hit
TATCAACGCAGAGTACTTTTTTTTT30550.13125225824773293No Hit
GGTATCAACGCAGAGTACTTTTTTT29900.12845965700841946No Hit
GTCCTACAGTGTGCATTTCTCATTT27880.11978111161855302No Hit
GTACTGGTTCACTATCGGTCAGTCA27870.11973814852256356No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATA400.0052872914.24709611
TAAGACG551.9627689E-413.8165554
ATCGCCG551.963127E-413.81625719
TAGGACC7600.013.6233964
GGTATCA17600.012.9882241
CGCTACT550.003071939812.0894852
ACCGTCC550.003071939812.0894858
GACGTGG10350.011.1967497
GCAACCG600.005886064411.0810759
TCCAACG1553.1286618E-1011.03119718
GTCCTAA13750.011.0140141
GTATCAA40000.010.9772151
TGTAGGA22100.010.9602482
GTCCTAC26250.010.9216531
GTAGGAC20900.010.9078063
GATCGTG1053.4729146E-610.8558657
CCGTCCA700.001494423110.854939
AGCGCAT700.001494423110.8549310
TCGTCTC803.7785506E-410.68555216
CTGTAGG21500.010.6765211
Produced by FastQC (version 0.11.5)