##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512881_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2327579
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.757066462620603	31.0	30.0	33.0	22.0	34.0
2	28.919244416623453	31.0	30.0	34.0	22.0	34.0
3	29.133963659235626	31.0	30.0	34.0	22.0	34.0
4	32.55087754271713	35.0	33.0	37.0	25.0	37.0
5	32.79314558173965	35.0	33.0	37.0	26.0	37.0
6	32.683334486176406	37.0	33.0	37.0	25.0	37.0
7	32.606113906337875	36.0	33.0	37.0	25.0	37.0
8	32.56654833197928	36.0	33.0	37.0	24.0	37.0
9	33.88115677276689	39.0	34.0	39.0	23.0	39.0
10	33.73499030537739	38.0	34.0	39.0	22.0	39.0
11	33.826293758450305	38.0	34.0	39.0	23.0	39.0
12	33.712280012837375	38.0	34.0	39.0	22.0	39.0
13	33.70400360202597	38.0	34.0	39.0	22.0	39.0
14	34.55896448627522	39.0	34.0	41.0	18.0	41.0
15	34.73165250244997	39.0	34.0	41.0	21.0	41.0
16	34.69924672803802	39.0	34.0	41.0	19.0	41.0
17	34.45049727635453	39.0	34.0	41.0	17.0	41.0
18	34.458888398632226	39.0	34.0	41.0	17.0	41.0
19	34.562499489813234	39.0	34.0	41.0	18.0	41.0
20	34.51916476304349	39.0	34.0	41.0	18.0	41.0
21	34.42193068420019	39.0	34.0	41.0	18.0	41.0
22	34.439740176380695	39.0	34.0	41.0	18.0	41.0
23	34.10225904255022	39.0	34.0	41.0	10.0	41.0
24	34.167615363431274	39.0	34.0	41.0	9.0	41.0
25	33.889834458894846	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5130330798665454
1101	2	0.23278494035275799
1101	3	0.4431248412363544
1101	4	0.6329191044160325
1101	5	0.6197788060946934
1101	6	0.6693384922302457
1101	7	0.46321069567522244
1101	8	0.6873685248551524
1101	9	0.7395715397953424
1101	10	0.798208514112531
1101	11	0.6259334519273665
1101	12	0.577476776115823
1101	13	0.9216572483437631
1101	14	1.0252020872906797
1101	15	0.860622089125151
1101	16	0.7496284539411349
1101	17	0.13588735565499377
1101	18	0.7270775648991474
1101	19	0.12918764681069206
1101	20	-0.3822138218765332
1101	21	0.5565800189516281
1101	22	0.6063275203420702
1101	23	0.22020176563043492
1101	24	0.7005926638874129
1101	25	0.34585010109422853
1102	1	0.8431598845777941
1102	2	0.8552793995726162
1102	3	0.9175899184906591
1102	4	1.048202475743281
1102	5	0.9553970780045233
1102	6	0.8291148821745153
1102	7	0.8505130481321572
1102	8	0.8938495753870654
1102	9	1.128611560058765
1102	10	1.1571520456474857
1102	11	0.9144735355139915
1102	12	1.019831765984108
1102	13	1.0184394798533916
1102	14	1.1437874469664635
1102	15	1.0931716395304178
1102	16	1.041456040693916
1102	17	1.2480727976509414
1102	18	1.2628966505121113
1102	19	1.0501511884773578
1102	20	1.18781594002823
1102	21	1.3334193996507153
1102	22	1.2153988855902966
1102	23	1.2036820879455306
1102	24	0.5323741830110151
1102	25	0.9822241354154073
1103	1	0.5539853423193684
1103	2	0.6409490859413154
1103	3	0.7965831916039967
1103	4	0.914389209939884
1103	5	0.6495070273570676
1103	6	0.45852967349308926
1103	7	0.2951212048085736
1103	8	0.57041348951266
1103	9	0.6158539716432827
1103	10	0.7487389773865587
1103	11	0.5239290318938288
1103	12	0.650736284224152
1103	13	0.7838752045818751
1103	14	0.9095299281694693
1103	15	0.8648001465618904
1103	16	0.941624756754905
1103	17	0.5504161125353733
1103	18	0.8177930059841287
1103	19	0.8021478250340266
1103	20	0.8892091800727826
1103	21	0.7863148761892802
1103	22	0.8539766668490003
1103	23	0.26706265307363
1103	24	0.33960982854847543
1103	25	0.7481999473410568
1104	1	1.092284356231275
1104	2	1.020355573568274
1104	3	0.998033705329501
1104	4	1.1715410925417942
1104	5	1.0520654048425868
1104	6	0.8788221709328567
1104	7	1.041679058001094
1104	8	0.7886883206367941
1104	9	1.1689307007443617
1104	10	1.1160425058421524
1104	11	0.8442264729267421
1104	12	1.2167765658910454
1104	13	1.2320647609919035
1104	14	1.4976002668826993
1104	15	1.1968941800531425
1104	16	1.258750361211213
1104	17	1.454233972422152
1104	18	1.3210741458587876
1104	19	0.9029118596228543
1104	20	1.0529979045078335
1104	21	1.09434562987407
1104	22	0.9001081011168992
1104	23	1.205231682233574
1104	24	0.9825572259961248
1104	25	0.6010745333718219
1105	1	0.5603854421858934
1105	2	0.7242992237379156
1105	3	0.9038100710631838
1105	4	0.9966878684635887
1105	5	0.7053938709027179
1105	6	0.9517982856364782
1105	7	0.8406276913313917
1105	8	0.8485321578309595
1105	9	0.5722007773548796
1105	10	0.7236235067175514
1105	11	0.7709450393454134
1105	12	0.9718791797772113
1105	13	0.9636493525955885
1105	14	1.1128111196539265
1105	15	1.0948628512162415
1105	16	1.0083576810195893
1105	17	1.3548457690521474
1105	18	1.143665157627602
1105	19	0.8058159993011103
1105	20	1.1642886411776558
1105	21	1.0716450598860732
1105	22	0.9902560626565275
1105	23	1.190818402600705
1105	24	1.2901278861445675
1105	25	1.4587406669561815
1106	1	0.5591544475294334
1106	2	0.6220052715848468
1106	3	0.5754358572066138
1106	4	0.7416302481168273
1106	5	0.5988372270456566
1106	6	0.6644281204318858
1106	7	0.5945863614312117
1106	8	0.6089918853509317
1106	9	0.8071482471071292
1106	10	0.6204031139493225
1106	11	0.4809297264534038
1106	12	0.7450002623709935
1106	13	0.7422747702925179
1106	14	0.9393856959936997
1106	15	0.7848992486188067
1106	16	0.8416260570224381
1106	17	0.94246681863482
1106	18	0.8947103317238998
1106	19	0.7749538131994242
1106	20	0.9783679828297878
1106	21	0.8983708483722239
1106	22	0.8552726001539455
1106	23	1.0450381798716037
1106	24	0.7312531174885066
1106	25	0.9658923625554863
1107	1	0.7586400481237945
1107	2	0.7624353798854528
1107	3	0.80823719176081
1107	4	0.8326875177374262
1107	5	0.6993119604016584
1107	6	0.7746895445839783
1107	7	0.5103180426420977
1107	8	0.7289969762770312
1107	9	0.6314336435847352
1107	10	0.868016405044159
1107	11	0.8089018468870819
1107	12	0.8922576983311572
1107	13	0.7052664659067105
1107	14	0.9217145158522726
1107	15	0.7688084142645835
1107	16	0.7587363058541925
1107	17	1.0432122885201096
1107	18	1.0128761831827902
1107	19	0.7545382401224501
1107	20	0.9038811080048035
1107	21	0.7500517534417455
1107	22	0.707518963799572
1107	23	1.008018332455471
1107	24	0.9011951564853575
1107	25	1.1596695598837385
1108	1	0.8077837841241546
1108	2	0.760894476267989
1108	3	0.6823661515753905
1108	4	0.8129228451881545
1108	5	0.6543223937241933
1108	6	0.7540020135946293
1108	7	0.7548880120911008
1108	8	0.7600426425291502
1108	9	0.7648083154024548
1108	10	0.8345818737076129
1108	11	0.614235904805092
1108	12	0.690649561616894
1108	13	0.7775014031964602
1108	14	0.9270461352641703
1108	15	0.7754402799656077
1108	16	0.6710029204993901
1108	17	0.9290031134667203
1108	18	1.0071889482977525
1108	19	0.9679748732190134
1108	20	1.0495264005848952
1108	21	1.0485466726401
1108	22	1.011744082038092
1108	23	1.2918778605697057
1108	24	1.1837805651615625
1108	25	1.10834771539772
1109	1	0.7793634273134131
1109	2	0.7690729031507963
1109	3	0.7811876348692728
1109	4	0.8203065974013981
1109	5	0.7753619924320105
1109	6	0.821252274997903
1109	7	0.784348894911794
1109	8	0.7070273408812611
1109	9	0.868197706426578
1109	10	0.9137297993269371
1109	11	0.849777258846423
1109	12	0.7305603981742266
1109	13	0.8776224902503671
1109	14	1.0109981545008964
1109	15	0.9026789224766816
1109	16	0.5217691947244347
1109	17	0.9769824232923128
1109	18	1.008712964986863
1109	19	0.9724138597911605
1109	20	0.9906658252564213
1109	21	0.8929414510279727
1109	22	0.8635916515908377
1109	23	1.0859919338475024
1109	24	0.9553594525559816
1109	25	0.607534799910411
1110	1	0.5987371008066873
1110	2	0.6872645828344766
1110	3	0.6101017702060183
1110	4	0.6528681987398031
1110	5	0.6068378152398495
1110	6	0.6170534584610365
1110	7	0.4823398246330939
1110	8	0.42972771211314154
1110	9	0.6438722140389359
1110	10	0.5838907509208724
1110	11	0.5983782359964351
1110	12	0.5044580391012374
1110	13	0.563138873447599
1110	14	0.5483455413111429
1110	15	0.5963282580643252
1110	16	0.7100765303414676
1110	17	0.6581536657158082
1110	18	0.08688192590744848
1110	19	0.5293305932951
1110	20	0.5270723391169412
1110	21	0.6671907159868127
1110	22	0.7125280902271243
1110	23	0.8243177879924346
1110	24	0.6809797219080949
1110	25	0.8007719652532543
1111	1	0.4651110277687138
1111	2	0.37645868028022633
1111	3	0.5241830843785102
1111	4	0.39612261926798453
1111	5	0.4578331385852934
1111	6	0.3295837364802594
1111	7	0.33209421885979395
1111	8	0.547372501788935
1111	9	0.6180140967743881
1111	10	0.6118755311281276
1111	11	0.3039949807743412
1111	12	0.06049848828050841
1111	13	0.3296898970763493
1111	14	0.48690326806125483
1111	15	0.40134608045165265
1111	16	0.5011715989382424
1111	17	0.08717597688993095
1111	18	0.5113758728085074
1111	19	0.33256993430512694
1111	20	0.521640205715812
1111	21	0.49149579317541026
1111	22	0.268016959689092
1111	23	0.6444807570042741
1111	24	0.5951281674903228
1111	25	0.748109378239171
1112	1	0.356595531344432
1112	2	0.2687903560771119
1112	3	0.2237408236239986
1112	4	0.49824249141904176
1112	5	0.3115075248108212
1112	6	0.19382161684816168
1112	7	0.02092183954071203
1112	8	0.2175302089803992
1112	9	0.1249523094732865
1112	10	0.12119061296825606
1112	11	0.15349401315243227
1112	12	0.23311531777076766
1112	13	0.23486231452136508
1112	14	0.2544124941825956
1112	15	-0.08531450489429915
1112	16	0.19973058010050693
1112	17	0.4570906665978356
1112	18	0.2965257535943806
1112	19	0.3308093637153249
1112	20	0.055921203683134024
1112	21	0.1684589077529992
1112	22	0.0010488393184857614
1112	23	0.28646982898391116
1112	24	0.207001146021895
1112	25	0.12223281728252289
1113	1	-0.053462395799876106
1113	2	0.01728174687280415
1113	3	0.0346701670395575
1113	4	0.1683697462400957
1113	5	-0.050553736688840445
1113	6	-0.29854857858368433
1113	7	-0.2867573979622762
1113	8	-0.24343459411974777
1113	9	-0.3196666886906101
1113	10	-0.21608576378562816
1113	11	-0.131271072974954
1113	12	-0.30644594337549336
1113	13	-0.2821023562675862
1113	14	0.12025119806433793
1113	15	-0.28464479252556174
1113	16	-0.3558242922241348
1113	17	-0.1917931484769042
1113	18	-0.3284902375430292
1113	19	-0.7463536523071923
1113	20	-0.4290782966159057
1113	21	-0.26235017948206263
1113	22	-0.6972351861819561
1113	23	-4.462673788180979
1113	24	-1.9685628128027446
1113	25	-1.0552293690667796
1114	1	-0.9691553364049668
1114	2	-1.159334786770934
1114	3	-0.9707913803316863
1114	4	-1.0376604345616833
1114	5	-0.9376748184477215
1114	6	-1.4644324773502788
1114	7	-1.328728994195334
1114	8	-1.232160698881092
1114	9	-1.5432061106714983
1114	10	-1.183622765597434
1114	11	-1.3240744805234073
1114	12	-2.2885522390263446
1114	13	-1.5645822411589592
1114	14	-3.460074696482465
1114	15	-2.2250133589407994
1114	16	-2.186945079877546
1114	17	-1.9626247516152446
1114	18	-1.8161890770887865
1114	19	-1.8676088182542046
1114	20	-1.5053621234506878
1114	21	-1.4052927445379453
1114	22	-1.5147106318082137
1114	23	-5.644616111175665
1114	24	-3.091369119327524
1114	25	-3.089227783820597
1115	1	-1.8849268069444491
1115	2	-1.7210340212529758
1115	3	-1.5193600452451683
1115	4	-1.5921626902660435
1115	5	-1.5615950132057996
1115	6	-1.7293128792356605
1115	7	-2.3571329003537507
1115	8	-2.6693114902819026
1115	9	-3.910722032417244
1115	10	-4.747238452546416
1115	11	-3.4400143930195135
1115	12	-3.6217247255400267
1115	13	-2.8353136843735456
1115	14	-3.4878645402154547
1115	15	-3.2598949052285775
1115	16	-3.2695693199331437
1115	17	-3.2006049525007505
1115	18	-6.004508078999812
1115	19	-3.9781833823017756
1115	20	-4.119716697728229
1115	21	-3.7217354756289716
1115	22	-3.2914347187367525
1115	23	-3.931638610009454
1115	24	-3.605768098763985
1115	25	-3.8459717486008635
1116	1	-1.6022230713280337
1116	2	-1.4921411660698425
1116	3	-1.9451738235284957
1116	4	-1.7868296970420303
1116	5	-1.8417775154711364
1116	6	-4.175869717286776
1116	7	-2.7243613158941784
1116	8	-3.1257100440263414
1116	9	-4.174969844570121
1116	10	-3.6856474231274525
1116	11	-3.072925456904205
1116	12	-2.949693872483568
1116	13	-4.081269967473027
1116	14	-7.268228714246625
1116	15	-5.256695554204615
1116	16	-4.094538509884693
1116	17	-4.870636674615618
1116	18	-3.70915168348629
1116	19	-3.443251831774038
1116	20	-4.1725117734594015
1116	21	-4.84975660294306
1116	22	-4.584382888152721
1116	23	-3.6478900763601345
1116	24	-4.568748435205265
1116	25	-4.3851931256580166
1201	1	0.5772949689856652
1201	2	0.6470419441094322
1201	3	1.0442269116192655
1201	4	0.9646455746574318
1201	5	0.4420976686475484
1201	6	0.9542268869675254
1201	7	1.0302303718936088
1201	8	1.0285688078692488
1201	9	1.5646694155049872
1201	10	1.2649331312854244
1201	11	1.4898971654766697
1201	12	1.3414777194961545
1201	13	1.3341074260698775
1201	14	1.4977172908755207
1201	15	1.4662934053091803
1201	16	1.5285503049881157
1201	17	1.8006514263411333
1201	18	1.5849323011738008
1201	19	1.72542676003318
1201	20	1.6570696302216419
1201	21	1.806226756708739
1201	22	1.640038879652053
1201	23	1.9560779301155335
1201	24	1.7212029972712841
1201	25	1.8735231524242266
1202	1	0.9822927035290725
1202	2	0.8865700426362011
1202	3	1.0266881585814929
1202	4	1.208066482762277
1202	5	1.063363441030944
1202	6	1.265764926043225
1202	7	1.3090226089290198
1202	8	1.2517320717743488
1202	9	1.4787663907771815
1202	10	1.3199020327449276
1202	11	1.258537067718784
1202	12	1.4886307957116074
1202	13	1.399724316765031
1202	14	1.9320667615824831
1202	15	1.6393389341216675
1202	16	1.4080238952562638
1202	17	1.702877065817745
1202	18	1.6484677178118687
1202	19	1.7958543134773564
1202	20	1.5964374305319424
1202	21	1.7314330505590547
1202	22	1.688872512757925
1202	23	1.8046997773551112
1202	24	1.8015683536344653
1202	25	2.0741410042221773
1203	1	1.011424301814131
1203	2	1.101168209388124
1203	3	1.0867429460716842
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2211	17	0.46619554820465225
2211	18	0.07838149270480699
2211	19	0.2020558638649561
2211	20	0.08228230215447496
2211	21	0.3562296502044262
2211	22	0.2941727643278398
2211	23	0.37710358227000995
2211	24	0.03767119643848105
2211	25	0.524714878078754
2212	1	0.27437120395653736
2212	2	0.4009902853342062
2212	3	0.05112798810592167
2212	4	0.3512244916688587
2212	5	0.0044728082552296655
2212	6	-0.06259354478070378
2212	7	0.1050666441980681
2212	8	0.12239106959236068
2212	9	0.24566848216535675
2212	10	-0.029321193275563928
2212	11	-0.19317438706563195
2212	12	0.14621638440101492
2212	13	0.2343882144796865
2212	14	0.38390294680878156
2212	15	0.0016279131822685144
2212	16	0.0010600447541548874
2212	17	-0.05192744872243793
2212	18	0.31403637378552673
2212	19	-0.03212581897297184
2212	20	0.1057883856289834
2212	21	0.23703443631107035
2212	22	0.28498704784195183
2212	23	0.2920605322454364
2212	24	0.33016384419833855
2212	25	0.44180966147185785
2213	1	-0.2679523501637142
2213	2	-0.13343516316763626
2213	3	-0.36149688869926777
2213	4	-0.17653116256376933
2213	5	-0.1940172143289658
2213	6	-0.07924575537745682
2213	7	-0.2783369692738944
2213	8	-0.09479956141963441
2213	9	-0.4187770411807392
2213	10	-0.20385278024291864
2213	11	-0.3903365423474625
2213	12	-0.3744362480584087
2213	13	-0.44341735410754524
2213	14	-0.42162745457899575
2213	15	-0.27541539320277053
2213	16	-0.170384398478042
2213	17	-0.18020597627625534
2213	18	-0.25288341048263163
2213	19	-0.2925037315528485
2213	20	-0.04801283340865581
2213	21	-0.23627684015080774
2213	22	-0.5136862667985014
2213	23	-0.32209741531994496
2213	24	-0.2359467638294177
2213	25	0.002578342532764566
2214	1	-2.8455191607135077
2214	2	-1.6315266409301756
2214	3	-1.720872147550839
2214	4	-3.4178352836342114
2214	5	-1.9758557277616404
2214	6	-1.6434674826499034
2214	7	-1.5525736063969724
2214	8	-1.3969390943056723
2214	9	-1.2752491741790593
2214	10	-1.2579937918425372
2214	11	-1.2139869094181677
2214	12	-1.1424313026511683
2214	13	-1.537135889260739
2214	14	-1.4752119544700903
2214	15	-1.8471983475007079
2214	16	-1.6599016383763114
2214	17	-3.6941003617664556
2214	18	-3.435687996335453
2214	19	-2.3678328373606305
2214	20	-2.1099419410571656
2214	21	-2.0338348152335612
2214	22	-3.0827069104990876
2214	23	-1.9492867244048284
2214	24	-2.9734970385572197
2214	25	-2.8874277625295264
2215	1	-1.7082538359730783
2215	2	-3.3389246980425824
2215	3	-2.0233179940377326
2215	4	-3.5944997825043963
2215	5	-2.3016494950456767
2215	6	-2.055936452299246
2215	7	-2.2971305554642925
2215	8	-1.5563675788503382
2215	9	-3.8473166649094743
2215	10	-2.290422295957587
2215	11	-1.868817633115949
2215	12	-1.7972979304781447
2215	13	-1.8337636108614745
2215	14	-1.524949560771148
2215	15	-1.7296107436835726
2215	16	-1.7590504019043394
2215	17	-2.1819697339635624
2215	18	-1.899425126925621
2215	19	-1.930742291211402
2215	20	-1.8143668803513364
2215	21	-2.414505597702714
2215	22	-2.2239179259160338
2215	23	-2.180459637787809
2215	24	-2.015361629558754
2215	25	-3.024180343185858
2216	1	-3.0299309245711115
2216	2	-3.157496074743335
2216	3	-4.452614201005616
2216	4	-5.0386327213582724
2216	5	-4.822309236758734
2216	6	-3.8023376758588903
2216	7	-5.476631716184901
2216	8	-3.3338997228189235
2216	9	-4.298323501523946
2216	10	-3.150255770424863
2216	11	-3.492493815507469
2216	12	-3.7443965602198865
2216	13	-3.51290098127944
2216	14	-4.443772015383267
2216	15	-3.9873574947162034
2216	16	-4.303307613751468
2216	17	-4.044120406586096
2216	18	-3.5319807141534234
2216	19	-4.088657818685782
2216	20	-4.806361128708403
2216	21	-4.441017494202079
2216	22	-4.041606954626534
2216	23	-4.142844694311641
2216	24	-4.322588243928653
2216	25	-4.457721628237888
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	114331.0
3	1.0
4	1025.0
5	6371.0
6	7279.0
7	6418.0
8	5712.0
9	5427.0
10	4782.0
11	4615.0
12	4314.0
13	4126.0
14	4273.0
15	4422.0
16	4537.0
17	4998.0
18	5526.0
19	6819.0
20	8861.0
21	11228.0
22	13988.0
23	17094.0
24	19441.0
25	22551.0
26	24706.0
27	27951.0
28	31401.0
29	37063.0
30	44943.0
31	56030.0
32	69931.0
33	89156.0
34	115961.0
35	151976.0
36	208120.0
37	323692.0
38	858510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.96737514786369	18.281341067653457	12.275057507337143	24.476226277145706
2	15.167686725824941	19.93235985143401	41.61802694606147	23.281926476679573
3	19.54106404736738	25.489743655847448	28.39675337509775	26.572438921687418
4	12.374366380312319	16.15367659475367	37.83198835733895	33.63996866759506
5	13.194174995069345	38.82086232107308	34.591026336787586	13.393936347069987
6	32.04107545736263	35.962696357309355	18.529835045455698	13.46639313987232
7	29.114251708753425	31.4540055519437	21.361782744944257	18.06995999435862
8	26.015477256999624	36.06335069324228	20.541751305003807	17.379420744754288
9	28.095399857239915	14.58135174881105	19.564194738516843	37.7590536554322
10	16.372339400778184	28.472281948692192	33.21831448652628	21.937064164003345
11	35.00395182811827	22.180205082248765	24.23304863543676	18.582794454196204
12	24.485217086148083	24.451801353143658	30.809649878808838	20.253331681899425
13	29.540059021810034	21.727258693633978	25.523858339055916	23.20882394550007
14	21.635260206830683	22.16706507955142	27.159503190098206	29.03817152351969
15	23.723171047392334	30.650903908983878	23.123102074935737	22.50282296868805
16	22.469006798508715	27.63049825629647	27.44675361533599	22.453741329858826
17	21.923298410067467	28.33442213184024	27.204107357969853	22.53817210012244
18	21.82980562669746	26.923508654224538	29.546720340276927	21.699965378801075
19	23.851851023606336	26.431638303398948	27.303436792029274	22.413073880965445
20	24.551634688492328	26.4789690608153	27.69750264047862	21.27189361021375
21	24.14863330270381	26.17237469379436	26.45328625244717	23.225705751054655
22	24.1130264920087	27.70341233311858	26.55303691712463	21.63052425774809
23	23.00642149282076	27.265283514154635	27.336069592261868	22.392225400762737
24	23.608087158636202	26.252045333843636	28.00106647630638	22.138801031213784
25	23.213986230503604	26.818738899902385	27.735644590462837	22.23163027913117
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	309.0
1	309.0
2	664.0
3	1019.0
4	1019.0
5	1019.0
6	1626.0
7	2233.0
8	2233.0
9	2233.0
10	2624.0
11	3015.0
12	3015.0
13	3015.0
14	4575.5
15	6136.0
16	6136.0
17	6136.0
18	12799.5
19	19463.0
20	19463.0
21	19463.0
22	33543.5
23	47624.0
24	47624.0
25	47624.0
26	73542.5
27	99461.0
28	99461.0
29	99461.0
30	131392.0
31	163323.0
32	163323.0
33	163323.0
34	186824.5
35	210326.0
36	210326.0
37	210326.0
38	229663.5
39	249001.0
40	249001.0
41	249001.0
42	265533.0
43	282065.0
44	282065.0
45	282065.0
46	300330.0
47	318595.0
48	318595.0
49	318595.0
50	308034.5
51	297474.0
52	297474.0
53	297474.0
54	265303.0
55	233132.0
56	233132.0
57	233132.0
58	202226.0
59	171320.0
60	171320.0
61	171320.0
62	141826.5
63	112333.0
64	112333.0
65	112333.0
66	86290.5
67	60248.0
68	60248.0
69	60248.0
70	45296.0
71	30344.0
72	30344.0
73	30344.0
74	21589.5
75	12835.0
76	12835.0
77	12835.0
78	8899.0
79	4963.0
80	4963.0
81	4963.0
82	3333.5
83	1704.0
84	1704.0
85	1704.0
86	1098.5
87	493.0
88	493.0
89	493.0
90	306.0
91	119.0
92	119.0
93	119.0
94	73.0
95	27.0
96	27.0
97	27.0
98	22.0
99	17.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3383343809168239
2	0.011299294245222182
3	0.01026817994147567
4	0.011857814493084875
5	0.012974854988810262
6	0.08197358714784761
7	0.08193062405185818
8	0.1069781090137005
9	0.08588322888288646
10	0.09292917662515429
11	0.08906249798610488
12	0.0999751243674221
13	0.08584026578689703
14	0.09030842776979858
15	0.08648471222673859
16	0.08889064560214711
17	0.10250994703079896
18	0.10371291371850322
19	0.09756919099201358
20	0.09636622430430933
21	0.08317655383555188
22	0.10418550777438704
23	0.09722548622409809
24	0.09297213972114372
25	0.09232769328130216
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2327579.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.2075059781486
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52935073131715	39.123133686059425
2	14.254335994482734	15.168753352819579
3	5.245415064007395	8.372863601281326
4	2.455472431017772	5.2259825621022875
5	1.3294961204260753	3.536958638774789
6	0.808234100661813	2.5802472445624187
7	0.5308004598071211	1.9769798046873996
8	0.35950530853153345	1.530270468229418
9	0.24247880352159001	1.161152314915439
>10	1.1355148381187357	10.22896817609446
>50	0.06234372889685866	2.3067025352128256
>100	0.039392986614549964	4.095736128510148
>500	0.005051966180862521	1.911619475124936
>1k	0.002525983090430734	2.542958556970903
>5k	8.148332549776561E-5	0.23767345465466105
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	5482	0.23552369221409886	No Hit
GTCCTACAGTGGACATTTCTAAATT	4726	0.20304359164608374	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4564	0.1960835700957948	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3998	0.17176645776577293	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3597	0.1545382562740083	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3055	0.13125225824773293	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2990	0.12845965700841946	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2788	0.11978111161855302	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2787	0.11973814852256356	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCATA	40	0.00528729	14.247096	11
TAAGACG	55	1.9627689E-4	13.816555	4
ATCGCCG	55	1.963127E-4	13.816257	19
TAGGACC	760	0.0	13.623396	4
GGTATCA	1760	0.0	12.988224	1
CGCTACT	55	0.0030719398	12.089485	2
ACCGTCC	55	0.0030719398	12.089485	8
GACGTGG	1035	0.0	11.196749	7
GCAACCG	60	0.0058860644	11.081075	9
TCCAACG	155	3.1286618E-10	11.031197	18
GTCCTAA	1375	0.0	11.014014	1
GTATCAA	4000	0.0	10.977215	1
TGTAGGA	2210	0.0	10.960248	2
GTCCTAC	2625	0.0	10.921653	1
GTAGGAC	2090	0.0	10.907806	3
GATCGTG	105	3.4729146E-6	10.855865	7
CCGTCCA	70	0.0014944231	10.85493	9
AGCGCAT	70	0.0014944231	10.85493	10
TCGTCTC	80	3.7785506E-4	10.685552	16
CTGTAGG	2150	0.0	10.676521	1
>>END_MODULE