FastQCFastQC Report
Thu 26 May 2016
SRR1512881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2327579
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68250.293223130127914No Hit
TATCAACGCAGAGTACTTTTTTTTT47900.2057932297894078No Hit
GTCCTACAGTGGACATTTCTAAATT44600.19161540811289327No Hit
GTCCTAAAGTGTGTATTTCTCATTT41660.1789842578919985No Hit
CTTTAGGACGTGAAATATGGCGAGG39400.16927459819838553No Hit
CTGTAGGACGTGGAATATGGCAAGA38730.1663960707670932No Hit
GGTATCAACGCAGAGTACTTTTTTT38440.1651501409833995No Hit
GTCCTACAGTGTGCATTTCTCATTT27740.11917962827470087No Hit
GTACTGGTTCACTATCGGTCAGTCA25660.11024330430889778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19800.012.8773871
TAGGACC8500.012.7565094
GATATAC4000.011.1788211
GTCCTAT2750.011.0707081
GGCGAGG10650.010.8718519
TTAGGAC16650.010.7407923
TCCAACG1951.8189894E-1210.70779318
GTCCTAC22450.010.5945331
AGGACCG1355.5915734E-810.5682575
GTATAGG2350.010.5260071
TAGTACC1451.4175384E-810.4953734
ACTGTTC5650.010.4256528
ATTACAC2100.010.4184043
AGGACGG1105.962045E-610.3761075
CGTGCGC1409.75233E-810.17439210
TCCTATA3000.010.1466212
GTATCAA40100.010.1070251
GCGCCAC1606.2591425E-910.08548513
TTTAGGA17800.010.0468642
CCCGTAC1804.0017767E-1010.01849116
Produced by FastQC (version 0.11.5)