##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512881_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2327579
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.237279164316227	31.0	30.0	34.0	26.0	34.0
2	30.227242125831175	31.0	30.0	34.0	26.0	34.0
3	30.418797385609682	31.0	30.0	34.0	26.0	34.0
4	34.02632606669849	37.0	35.0	37.0	30.0	37.0
5	33.59653958039663	37.0	35.0	37.0	28.0	37.0
6	33.467279520909926	37.0	35.0	37.0	28.0	37.0
7	33.30571550954876	37.0	35.0	37.0	27.0	37.0
8	33.245132388632136	37.0	35.0	37.0	27.0	37.0
9	34.577836026188585	39.0	35.0	39.0	27.0	39.0
10	34.493137719493085	39.0	35.0	39.0	27.0	39.0
11	34.48532402122549	39.0	35.0	39.0	27.0	39.0
12	34.34104148559512	39.0	35.0	39.0	26.0	39.0
13	34.33776640878784	39.0	35.0	39.0	26.0	39.0
14	35.415979436143736	40.0	36.0	41.0	26.0	41.0
15	35.40759690648524	40.0	36.0	41.0	26.0	41.0
16	35.40151204320025	40.0	36.0	41.0	26.0	41.0
17	35.38712885792491	40.0	36.0	41.0	26.0	41.0
18	35.30622247408144	40.0	36.0	41.0	25.0	41.0
19	35.32145976570505	40.0	35.0	41.0	25.0	41.0
20	35.28918932504546	40.0	35.0	41.0	25.0	41.0
21	35.254780181467524	40.0	35.0	41.0	25.0	41.0
22	35.21695289397267	40.0	36.0	41.0	25.0	41.0
23	35.04973536881025	40.0	35.0	41.0	24.0	41.0
24	35.00617293763176	40.0	35.0	41.0	24.0	41.0
25	34.94961674770223	40.0	35.0	41.0	24.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.37333631806043144
1101	2	0.26533527465798556
1101	3	0.22857746711896354
1101	4	0.29684662086948066
1101	5	0.401320152790106
1101	6	0.3662759902854731
1101	7	0.37032727646288066
1101	8	0.22171485704028981
1101	9	0.4288469818069629
1101	10	0.17614620977874296
1101	11	0.5135553913266477
1101	12	0.5744561068249467
1101	13	0.622947974068957
1101	14	0.6181852161295609
1101	15	0.2633289182395586
1101	16	0.5238208181906856
1101	17	0.510079978045674
1101	18	0.40138353879410715
1101	19	0.2604069193148675
1101	20	0.4400070236371576
1101	21	0.5627109713526934
1101	22	0.473238889743655
1101	23	0.564433721377064
1101	24	0.5510979711795017
1101	25	0.6825042983144414
1102	1	0.7408709875133184
1102	2	0.8370364524695368
1102	3	0.5270581060500703
1102	4	0.6396202562544886
1102	5	0.8168512761437086
1102	6	0.6712251734161683
1102	7	0.7401220301356304
1102	8	0.7624042092439396
1102	9	0.9307475485496184
1102	10	0.8721875598293991
1102	11	0.8909311195434668
1102	12	0.9043988784814871
1102	13	0.9523851891145512
1102	14	0.9123633601265269
1102	15	0.9942148098302397
1102	16	0.9256287747509688
1102	17	0.9986928833141704
1102	18	0.5247692720767816
1102	19	0.8560486208092968
1102	20	1.017222860769884
1102	21	0.8451157490882579
1102	22	0.6082978594751651
1102	23	0.8767905317873996
1102	24	0.8903374595280269
1102	25	1.0147028798747257
1103	1	0.6417527492092248
1103	2	0.7901288188020317
1103	3	0.47174567131740375
1103	4	0.43530032012944986
1103	5	0.6414137674413922
1103	6	0.6249778276211302
1103	7	0.7090966585469332
1103	8	0.8016336848426562
1103	9	0.8122139097651555
1103	10	0.6667673252150905
1103	11	0.9152421140436076
1103	12	0.8985808394603225
1103	13	0.9473106538179792
1103	14	1.0158180689471905
1103	15	1.051880675884263
1103	16	0.9374786033154336
1103	17	0.9917854236928889
1103	18	0.9388437377548655
1103	19	0.9280433863690334
1103	20	0.8865043544345994
1103	21	0.9687881027153793
1103	22	0.9029118902117332
1103	23	0.9790584628999
1103	24	0.9386396385867855
1103	25	0.9445274412782325
1104	1	0.8369482569797384
1104	2	0.9892184266435713
1104	3	0.8721529054722801
1104	4	0.7082068444932048
1104	5	0.9626706737317292
1104	6	0.7721084573079011
1104	7	0.7230516509864131
1104	8	0.770598124533997
1104	9	0.8647311964672539
1104	10	1.0616397759764453
1104	11	0.9931134572586799
1104	12	0.9630208029230829
1104	13	0.8609757884243123
1104	14	1.20508035785803
1104	15	1.2350559956530986
1104	16	1.128679551759653
1104	17	1.122996096048773
1104	18	0.7309171170046511
1104	19	1.1820653849050018
1104	20	1.116454455891649
1104	21	1.0571811356052407
1104	22	0.922820057299738
1104	23	1.0028729756566364
1104	24	1.233571231049666
1104	25	0.9403272012645161
1105	1	0.531419444683543
1105	2	0.5711762650037429
1105	3	0.5584567017670921
1105	4	0.35989149154458033
1105	5	0.4380989670304061
1105	6	0.5850888331041801
1105	7	0.6287041062850491
1105	8	0.5176670633079823
1105	9	0.7981413720991526
1105	10	0.4478195726920191
1105	11	0.7278243358817207
1105	12	0.8017919323265872
1105	13	0.7727939021687433
1105	14	0.8516240590181496
1105	15	0.9178002641269032
1105	16	0.8034414030350376
1105	17	0.5616247730240715
1105	18	0.4873894314911169
1105	19	0.68646242246502
1105	20	0.8505283992208987
1105	21	0.676280012566636
1105	22	0.6932978167138089
1105	23	0.8038407250687314
1105	24	0.768151156846038
1105	25	0.5803843248665146
1106	1	-0.028130454259887472
1106	2	0.18225862450955788
1106	3	-1.4666200224240846
1106	4	-0.3866237074058816
1106	5	-0.1033800155461222
1106	6	-0.0444914573509223
1106	7	0.12566282536909057
1106	8	0.19368439094210288
1106	9	0.35534215840231553
1106	10	0.10361387305897551
1106	11	0.231565239193273
1106	12	0.39361923527692966
1106	13	0.3469251244953213
1106	14	0.36706367106315696
1106	15	0.4117123779236209
1106	16	0.3470032796842659
1106	17	0.5552216840367876
1106	18	0.4188445395865088
1106	19	0.5761893674046306
1106	20	0.578695814494175
1106	21	0.42660959115329433
1106	22	0.37258856785482664
1106	23	0.37970556270735045
1106	24	0.4050418091667183
1106	25	0.3946361761504349
1107	1	0.5176175730812034
1107	2	0.6129565191207895
1107	3	0.4956687476165662
1107	4	0.3058863050486025
1107	5	0.22540746257458721
1107	6	0.2931159452993555
1107	7	0.3117778491177532
1107	8	0.313847336555547
1107	9	0.2592984495936932
1107	10	0.18928789308568383
1107	11	0.23779877538412109
1107	12	0.48732075829732935
1107	13	0.27887581266980277
1107	14	0.34866812829188376
1107	15	0.33813095638594604
1107	16	0.41690397364525467
1107	17	0.3768797668863684
1107	18	0.3493853290578244
1107	19	0.3768190472039663
1107	20	0.47145851437598907
1107	21	0.40368873076879197
1107	22	0.3356408269008142
1107	23	0.48151673911903714
1107	24	0.5473026120939082
1107	25	0.5681009381271522
1108	1	0.3902930090802883
1108	2	0.2884903487481765
1108	3	0.30343986014433355
1108	4	0.1638743865597334
1108	5	0.41978513839958964
1108	6	0.36814210118028257
1108	7	0.3319489393506032
1108	8	0.3446447977223386
1108	9	0.5720528235188667
1108	10	0.37696780548525055
1108	11	0.36350928058452325
1108	12	0.3614256233492412
1108	13	0.3870157202155866
1108	14	0.46563775031182075
1108	15	0.4974176585154595
1108	16	0.4573434040566724
1108	17	0.4809618841599317
1108	18	0.4994065788967106
1108	19	0.47051051580007197
1108	20	0.4665100887800335
1108	21	0.5467219188368375
1108	22	0.658643313015844
1108	23	0.7119906918096959
1108	24	0.6698166422129432
1108	25	0.6035080467207976
1109	1	0.22915856684089064
1109	2	0.1838983718592182
1109	3	0.29112556402333567
1109	4	0.20885624312324325
1109	5	-0.12344945130701035
1109	6	0.23502163068054926
1109	7	0.31532973756878846
1109	8	0.333790057711397
1109	9	0.44354175679293917
1109	10	0.0957119825947288
1109	11	0.23905784955967135
1109	12	0.6081056491741634
1109	13	0.3928968941510078
1109	14	0.5414747858286617
1109	15	0.47013382819373106
1109	16	0.42425056367211056
1109	17	0.5221388224175953
1109	18	0.7302793736835866
1109	19	0.6635194426091928
1109	20	0.6032415871403956
1109	21	0.734617820888154
1109	22	0.621342076157589
1109	23	0.5606010612567474
1109	24	0.569706383373564
1109	25	0.4192397418785063
1110	1	0.4831370103624195
1110	2	0.3585182100412325
1110	3	0.47769707264749783
1110	4	0.46621615463963906
1110	5	0.5716307002257182
1110	6	0.605409415241418
1110	7	0.4893436069581938
1110	8	0.5362809137132487
1110	9	0.4944730284812735
1110	10	0.5776431179907533
1110	11	0.5449960903957844
1110	12	0.6498440297479036
1110	13	0.5925149716795488
1110	14	0.7560039516234411
1110	15	0.716176917844173
1110	16	0.5757513750927004
1110	17	0.5958790431908767
1110	18	0.5257795994018934
1110	19	0.5422446180285405
1110	20	0.6774712442461635
1110	21	0.7208208842731949
1110	22	0.7134006929983556
1110	23	0.6972480373154042
1110	24	0.6736437112031837
1110	25	0.7327409393761997
1111	1	0.049155992519995806
1111	2	-0.06476386461991623
1111	3	-0.04101129898998579
1111	4	0.21361318432524712
1111	5	0.3632037043813554
1111	6	0.24807242808906693
1111	7	0.13278464278582902
1111	8	0.09086350914380859
1111	9	0.1467558721477502
1111	10	0.09961788359441925
1111	11	0.06126969597870868
1111	12	0.23136034794076465
1111	13	0.2725130679530068
1111	14	0.1798225765817989
1111	15	0.1242006242494611
1111	16	0.17922367211411938
1111	17	0.37559333391497063
1111	18	0.17888204744393477
1111	19	0.05878925415375846
1111	20	0.1159054792612082
1111	21	0.2393669381737027
1111	22	0.047832342953135765
1111	23	0.08736869850969242
1111	24	0.21354757301267568
1111	25	0.1386014112498941
1112	1	-0.0674636639371613
1112	2	-0.43652144867876075
1112	3	-0.09230945150640224
1112	4	0.006804952796379382
1112	5	-0.0545782986277672
1112	6	-0.07160535134396895
1112	7	0.1312757033149765
1112	8	0.06261812785276533
1112	9	0.10410439748631006
1112	10	-0.16968311989410267
1112	11	-0.12687674147111494
1112	12	0.23159799955310234
1112	13	0.08911987456065162
1112	14	-0.28291653122914795
1112	15	-0.41182625876536605
1112	16	-0.4686659519148151
1112	17	-0.27268009111357117
1112	18	-0.09590012289564953
1112	19	-0.16126745463264314
1112	20	-0.14834376828125784
1112	21	-0.22237425879683315
1112	22	-0.062213627030459406
1112	23	0.10918230650032257
1112	24	0.16606907356444367
1112	25	-0.10110885994547658
1113	1	-0.733202950652565
1113	2	-0.5668629656577799
1113	3	-0.5885882696563876
1113	4	-2.1563442997513533
1113	5	-0.7950701626904504
1113	6	-0.4712750115515618
1113	7	-0.5464973295418858
1113	8	-0.42333419685927964
1113	9	-0.5274082008441638
1113	10	-0.561587130379479
1113	11	-0.6836584609258551
1113	12	-0.41782721712415594
1113	13	-0.4250141705390931
1113	14	-0.5051560227017902
1113	15	-0.5964116221780102
1113	16	-0.7503639202169552
1113	17	-0.8376891359129317
1113	18	-0.6305062164767037
1113	19	-0.5293825896911386
1113	20	-0.4281276769492095
1113	21	-0.4735207791748124
1113	22	-0.5516837899358009
1113	23	-0.39546936517960773
1113	24	-0.29005202284871956
1113	25	-0.48863847270479965
1114	1	-1.2148453879453136
1114	2	-2.4138207125795823
1114	3	-1.7162526315372197
1114	4	-1.2034924179525177
1114	5	-1.167914395089575
1114	6	-0.8083876444222327
1114	7	-0.9221752061004196
1114	8	-0.9635474720447732
1114	9	-1.2308971819658296
1114	10	-1.3280558738116568
1114	11	-1.3369803135156744
1114	12	-1.4460577984674643
1114	13	-1.4808510235043002
1114	14	-1.377662410304886
1114	15	-1.614579500147272
1114	16	-1.9705147068494213
1114	17	-1.981374715908622
1114	18	-1.6746297877231981
1114	19	-2.1938693767594515
1114	20	-1.474834415485688
1114	21	-1.7866759970996497
1114	22	-1.5529645217977617
1114	23	-1.3970867237803262
1114	24	-1.429078423022176
1114	25	-1.9667939442070193
1115	1	-1.0476248847704248
1115	2	-1.280999581794184
1115	3	-0.8043057083484655
1115	4	-0.547586652781689
1115	5	-0.7518576303847908
1115	6	-0.6887650435526211
1115	7	-0.5382888316955032
1115	8	-0.8710757263575246
1115	9	-1.8618018969403494
1115	10	-1.5152948413803529
1115	11	-1.7702223952371483
1115	12	-2.3449978296559095
1115	13	-2.337794419279774
1115	14	-1.957879263195366
1115	15	-2.163838325480164
1115	16	-1.837041391329464
1115	17	-1.7653959259884218
1115	18	-2.307826679845931
1115	19	-2.734698992984363
1115	20	-2.8142102081273066
1115	21	-2.1934652284563967
1115	22	-2.221256005266575
1115	23	-2.0559457216020007
1115	24	-2.6732818709357744
1115	25	-2.702844239849192
1116	1	-1.090145198639913
1116	2	-1.2757416301322202
1116	3	-1.330264008375309
1116	4	-0.9506889391764801
1116	5	-1.6080894910151144
1116	6	-2.190614536280556
1116	7	-1.6408177768276602
1116	8	-2.0923241302412947
1116	9	-3.1404568733378504
1116	10	-1.765096707927981
1116	11	-2.5955095478547783
1116	12	-4.870997358050836
1116	13	-3.732176238459271
1116	14	-3.0146128345063588
1116	15	-4.090793669818606
1116	16	-3.519511326506013
1116	17	-3.315384580772026
1116	18	-3.224263673852988
1116	19	-3.5026558010781734
1116	20	-5.35976673919021
1116	21	-4.202227813860663
1116	22	-3.4435311883101463
1116	23	-4.1497847500362255
1116	24	-4.019790732810357
1116	25	-3.175799913421365
1201	1	0.52648586068943
1201	2	0.7137410461240989
1201	3	0.7739372151016468
1201	4	0.639746841553098
1201	5	7.737502699356469E-4
1201	6	0.6380147581633722
1201	7	0.8109998561027112
1201	8	0.6863701862968767
1201	9	0.6866148765526745
1201	10	0.7644694354656423
1201	11	0.5531312913283273
1201	12	1.0731635915371385
1201	13	1.0377726064033226
1201	14	0.9720314282890641
1201	15	0.9072672784423901
1201	16	0.9804136990383796
1201	17	0.7809211026898026
1201	18	1.0015955466780682
1201	19	1.0547603668646062
1201	20	1.1886511670983566
1201	21	0.7565000753098374
1201	22	0.8144761498976294
1201	23	0.8071546997972376
1201	24	0.5610672270882588
1201	25	0.8769356589721156
1202	1	0.8512246466792988
1202	2	0.9661949475149534
1202	3	0.832951672833719
1202	4	0.7279108606103932
1202	5	0.8029997329975487
1202	6	0.8588058144153479
1202	7	0.9387637131455335
1202	8	0.8861755629805188
1202	9	1.1350057158700153
1202	10	1.1381389945940583
1202	11	1.061108787792847
1202	12	1.1343195922510958
1202	13	1.0733021740443647
1202	14	1.1572136259192547
1202	15	1.1669775059491485
1202	16	1.217059862178715
1202	17	1.0962891364107819
1202	18	1.0453830468496932
1202	19	1.1638472505574384
1202	20	1.0058628019342137
1202	21	1.198240426524265
1202	22	1.016393893228262
1202	23	1.1876793246238933
1202	24	1.1868615098996216
1202	25	1.2973618394122965
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2211	7	-0.023069674598595213
2211	8	-0.1416677211270425
2211	9	0.14375497172238028
2211	10	0.026767778544794396
2211	11	0.2041102792887557
2211	12	0.06481684290562839
2211	13	0.3209093793138891
2211	14	0.13302322654401877
2211	15	0.2778007732853851
2211	16	0.2615122015228408
2211	17	0.10328348408935994
2211	18	0.06318721919748782
2211	19	0.16560920012823743
2211	20	0.0582968848562615
2211	21	0.013839361352928847
2211	22	0.15171866166232206
2211	23	-0.018841649137300465
2211	24	0.3072290708237091
2211	25	0.08557774144725983
2212	1	-0.09290951351159293
2212	2	-0.027628184080448648
2212	3	0.00446751455685046
2212	4	-0.023164181817101337
2212	5	-0.38599655379952935
2212	6	-0.040733544259644816
2212	7	-0.24203992094447102
2212	8	-0.03553327597365552
2212	9	-0.013480292628244683
2212	10	-0.29233319466445096
2212	11	-0.09704462746063314
2212	12	0.13927340051711923
2212	13	0.08409024075441351
2212	14	0.19816572432551283
2212	15	-0.020326147281082285
2212	16	-0.05560311680532237
2212	17	-0.03415115247895528
2212	18	-0.08384566603422883
2212	19	0.1743759672694054
2212	20	0.11110467053021011
2212	21	0.19056264576911985
2212	22	0.25164220569875795
2212	23	-0.03535210361941665
2212	24	-0.17682885236096268
2212	25	-0.02282471394426011
2213	1	-0.4042277979852287
2213	2	0.09961477972300514
2213	3	-0.040187649078589516
2213	4	-0.23491287531568616
2213	5	-0.008196713089056118
2213	6	0.06032438548567853
2213	7	-0.4016722094403491
2213	8	-0.38285990314524554
2213	9	-0.14373857441068338
2213	10	-0.20306605376595144
2213	11	0.0226266287809338
2213	12	-0.33549166561712696
2213	13	-0.1482233486786555
2213	14	-0.15509016447730062
2213	15	-0.4709718517371897
2213	16	-0.8061881785027367
2213	17	-0.5974737392244691
2213	18	-0.30752061721089774
2213	19	-0.18215935827401353
2213	20	-0.23510274770661965
2213	21	-0.1547183111317949
2213	22	-0.22120629468855668
2213	23	-0.3802048335436652
2213	24	-0.10890623506677599
2213	25	-0.09622518162993998
2214	1	-1.3661228522711966
2214	2	-0.6614958200763539
2214	3	-0.6933924999954222
2214	4	-0.8316945257686541
2214	5	-1.1949609993950006
2214	6	-1.7160335306939665
2214	7	-1.1760133075539159
2214	8	-1.0146704989563773
2214	9	-0.913361901270811
2214	10	-1.3170520151905478
2214	11	-1.1888056372402573
2214	12	-0.9221146764865935
2214	13	-1.452287970302848
2214	14	-0.9886351808750717
2214	15	-1.093866295516925
2214	16	-1.0421370248300335
2214	17	-1.144434860101633
2214	18	-1.084878198992655
2214	19	-1.178912784318399
2214	20	-1.3368617112572494
2214	21	-1.5550211099500473
2214	22	-1.8636429201537226
2214	23	-1.8115165936116426
2214	24	-1.7144834702405234
2214	25	-2.184738460907397
2215	1	-1.13996846301022
2215	2	-0.5973691635561806
2215	3	-0.686962875852192
2215	4	-0.7110952655292024
2215	5	-1.182436705772993
2215	6	-0.9449131713214811
2215	7	-1.3950105059780498
2215	8	-1.062792043089857
2215	9	-1.0256791251665547
2215	10	-1.1658975130235092
2215	11	-0.8678863259371496
2215	12	-1.1105126748878433
2215	13	-1.2503432762105433
2215	14	-1.3620238231269326
2215	15	-1.2970843442291198
2215	16	-1.2377774205131118
2215	17	-1.3410767325960578
2215	18	-1.0176506149867777
2215	19	-1.2691499472749683
2215	20	-1.2739997532251905
2215	21	-0.9762784620167579
2215	22	-1.1237423678172433
2215	23	-1.6232284904486178
2215	24	-1.606773566571981
2215	25	-1.766218704603851
2216	1	-1.3012686314666944
2216	2	-0.7402605688815953
2216	3	-0.7957465405407547
2216	4	-1.0814040921948802
2216	5	-1.7668151884738919
2216	6	-1.907892882395469
2216	7	-1.822364573550118
2216	8	-1.8943483686102525
2216	9	-3.787474521431573
2216	10	-1.8798434942675044
2216	11	-1.9404640215989488
2216	12	-2.476191417862271
2216	13	-2.3739135907549063
2216	14	-2.421485035081453
2216	15	-2.2783843401808213
2216	16	-1.7595510700476922
2216	17	-1.9721884270700798
2216	18	-2.422055426923883
2216	19	-2.545543351082067
2216	20	-1.7863004563073304
2216	21	-2.459989629488703
2216	22	-1.8429852193377982
2216	23	-2.9817240791260176
2216	24	-2.4117347342178235
2216	25	-2.486329644647732
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65487.0
3	3.0
4	1126.0
5	9704.0
6	11314.0
7	8672.0
8	6892.0
9	5974.0
10	5145.0
11	4746.0
12	4211.0
13	3936.0
14	3976.0
15	3862.0
16	3904.0
17	4174.0
18	4711.0
19	5660.0
20	7315.0
21	9407.0
22	12202.0
23	14830.0
24	17166.0
25	19733.0
26	22048.0
27	24480.0
28	27410.0
29	32759.0
30	41366.0
31	53034.0
32	66670.0
33	85763.0
34	111656.0
35	146591.0
36	201122.0
37	309771.0
38	970759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.07162821577906	19.00829218202552	13.262116200629182	26.657963401566242
2	13.580611468581965	20.32739745457828	40.541836984693944	25.550154092145817
3	17.57125208143095	25.401686630499004	29.29155073320084	27.735510554869208
4	11.590569463671759	16.797240547250215	36.97758632097887	34.63460366809915
5	12.840593000415543	38.954381046469805	34.706913940683634	13.498112012431024
6	32.070215742227035	36.42989731760182	18.09326963006652	13.406617310104627
7	28.69208816903867	31.538193956233666	21.4475490708265	18.322168803901157
8	26.087279858418157	36.402988969116876	20.107923055065864	17.4018081173991
9	27.487691376163664	14.408513523908404	19.495175603892864	38.60861949603507
10	15.80675125762387	28.970246443986035	33.17264232330401	22.05035997508609
11	34.82533965969796	22.117742393560547	24.203801404397947	18.85311654234355
12	24.019226861238103	24.703543642165222	30.910040784902066	20.36718871169461
13	29.37051759962465	21.625428153556104	25.68852591435382	23.315528332465426
14	21.448745852949745	22.03747446894986	27.252468037316362	29.261311640784033
15	23.616556292528994	30.754981445800944	23.03535180789572	22.593110453774347
16	22.48476166496373	27.637737963369563	27.241408053953116	22.636092317713594
17	21.717118986700008	28.417599556595324	27.296284122718262	22.56899733398641
18	21.701892170472792	26.922708408792005	29.632861611913206	21.742537808821993
19	23.633088996957717	26.43778015225589	27.38505084147179	22.544080009314598
20	24.40133376354268	26.423976466231213	27.790005709869053	21.384684060357053
21	23.809112589905958	26.032073779919067	26.792909861825915	23.365903768349064
22	23.92838883470744	27.59650622752484	26.72456682290973	21.750538114857985
23	22.717209598471204	27.188679739763934	27.561427560568298	22.532683101196564
24	23.520877272049628	26.21552265250718	28.11036703802535	22.15323303741785
25	22.94917721339521	26.81633010794912	27.826264039271724	22.408228639383942
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	71.0
1	71.0
2	267.5
3	464.0
4	464.0
5	464.0
6	1130.0
7	1796.0
8	1796.0
9	1796.0
10	2233.0
11	2670.0
12	2670.0
13	2670.0
14	4330.0
15	5990.0
16	5990.0
17	5990.0
18	13169.5
19	20349.0
20	20349.0
21	20349.0
22	34640.0
23	48931.0
24	48931.0
25	48931.0
26	75707.5
27	102484.0
28	102484.0
29	102484.0
30	133411.5
31	164339.0
32	164339.0
33	164339.0
34	188052.0
35	211765.0
36	211765.0
37	211765.0
38	230166.5
39	248568.0
40	248568.0
41	248568.0
42	266020.5
43	283473.0
44	283473.0
45	283473.0
46	300877.5
47	318282.0
48	318282.0
49	318282.0
50	308348.0
51	298414.0
52	298414.0
53	298414.0
54	265494.5
55	232575.0
56	232575.0
57	232575.0
58	201187.5
59	169800.0
60	169800.0
61	169800.0
62	140212.0
63	110624.0
64	110624.0
65	110624.0
66	84647.5
67	58671.0
68	58671.0
69	58671.0
70	44011.0
71	29351.0
72	29351.0
73	29351.0
74	20786.5
75	12222.0
76	12222.0
77	12222.0
78	8413.5
79	4605.0
80	4605.0
81	4605.0
82	3116.5
83	1628.0
84	1628.0
85	1628.0
86	1018.5
87	409.0
88	409.0
89	409.0
90	248.5
91	88.0
92	88.0
93	88.0
94	48.0
95	8.0
96	8.0
97	8.0
98	5.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08657063841871747
2	0.04506828769292041
3	0.01950524557920483
4	0.006917058454299511
5	0.0213956218027401
6	0.059160183177456055
7	0.08205951333982649
8	0.05550832001835383
9	0.02371562898616975
10	0.05018089611566352
11	0.007561504894141079
12	8.592619197887592E-5
13	0.006143722726489628
14	9.02225015778197E-4
15	0.014736341924377217
16	0.009666696597623538
17	0.005757054862584686
18	0.00627261201445794
19	0.0021481547994718977
20	0.0012888928796831386
21	3.4370476791550366E-4
22	3.8666786390494155E-4
23	0.0
24	0.0
25	4.296309598943796E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2327579.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.0600672102847
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.50120526005878	37.744176186653064
2	14.786335483679714	15.395552381483665
3	5.414250863974187	8.455987916155145
4	2.546983896682256	5.303846113791642
5	1.3793864796135125	3.590547641881873
6	0.8681755176082905	2.7118365478206785
7	0.5780185134059714	2.1064177859692603
8	0.36516817584907474	1.5208543822207914
9	0.26958640439717835	1.2631217698706483
>10	1.1756646949762781	10.394220697799751
>50	0.06506208810919556	2.350106485266735
>100	0.042623634145010504	4.3422088749569845
>500	0.004639376923434602	1.7382128415708649
>1k	0.002816764560656335	2.788550227575633
>5k	8.284601648989221E-5	0.29436014698327573
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6825	0.293223130127914	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4790	0.2057932297894078	No Hit
GTCCTACAGTGGACATTTCTAAATT	4460	0.19161540811289327	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4166	0.1789842578919985	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3940	0.16927459819838553	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3873	0.1663960707670932	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3844	0.1651501409833995	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2774	0.11917962827470087	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2566	0.11024330430889778	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.296309598943795E-5
3	0.0	0.0	0.0	0.0	4.296309598943795E-5
4	0.0	0.0	0.0	0.0	4.296309598943795E-5
5	0.0	0.0	0.0	0.0	4.296309598943795E-5
6	0.0	0.0	0.0	0.0	4.296309598943795E-5
7	0.0	0.0	0.0	0.0	4.296309598943795E-5
8	0.0	0.0	0.0	0.0	4.296309598943795E-5
9	0.0	0.0	0.0	0.0	4.296309598943795E-5
10	0.0	0.0	0.0	0.0	4.296309598943795E-5
11	0.0	0.0	0.0	4.296309598943795E-5	4.296309598943795E-5
12	0.0	0.0	0.0	4.296309598943795E-5	4.296309598943795E-5
13	0.0	0.0	0.0	4.296309598943795E-5	4.296309598943795E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1980	0.0	12.877387	1
TAGGACC	850	0.0	12.756509	4
GATATAC	400	0.0	11.178821	1
GTCCTAT	275	0.0	11.070708	1
GGCGAGG	1065	0.0	10.87185	19
TTAGGAC	1665	0.0	10.740792	3
TCCAACG	195	1.8189894E-12	10.707793	18
GTCCTAC	2245	0.0	10.594533	1
AGGACCG	135	5.5915734E-8	10.568257	5
GTATAGG	235	0.0	10.526007	1
TAGTACC	145	1.4175384E-8	10.495373	4
ACTGTTC	565	0.0	10.425652	8
ATTACAC	210	0.0	10.418404	3
AGGACGG	110	5.962045E-6	10.376107	5
CGTGCGC	140	9.75233E-8	10.174392	10
TCCTATA	300	0.0	10.146621	2
GTATCAA	4010	0.0	10.107025	1
GCGCCAC	160	6.2591425E-9	10.085485	13
TTTAGGA	1780	0.0	10.046864	2
CCCGTAC	180	4.0017767E-10	10.018491	16
>>END_MODULE