FastQCFastQC Report
Thu 26 May 2016
SRR1512880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776886
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT112100.4036896005093475No Hit
GTCCTACAGTGGACATTTCTAAATT100300.3611959583504688No Hit
CTGTAGGACGTGGAATATGGCAAGA83470.3005885009323393No Hit
GTCCTAAAGTGTGTATTTCTCATTT80560.290109136637226No Hit
CTTTAGGACGTGAAATATGGCGAGG66340.23890069667966204No Hit
TATCAACGCAGAGTACTTTTTTTTT62540.22521630344205704No Hit
GGTATCAACGCAGAGTACTTTTTTT61900.22291156352835512No Hit
GTCCTACAGTGTGCATTTCTCATTT44670.16086364366416195No Hit
CTGAAGGACCTGGAATATGGCGAGA33500.12063872985783357No Hit
CTGTAGGACCTGGAATATGGCGAGA30690.11051948117423618No Hit
ACGCAGAGTACTTTTTTTTTTTTTT30510.10987127307350752No Hit
GTACTGGTTCACTATCGGTCAGTCA29220.10522578168495214No Hit
GTCCTTCAGTGTGCATTTCTCATTT28640.10313711113815978No Hit
ATTTAGAAATGTCCACTGTAGGACG28160.10140855620288337No Hit
TTTCTAAATTTTCCACCTTTTTCAG28080.10112046371367064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC15500.013.359354
GGTATCA26450.012.8561431
TACGCTG550.00307282912.0891545
GGACCGT907.478433E-611.6094256
AGGACCT27550.011.3776575
GGCGAGG19400.011.01644219
TGTAGGA46550.010.9984032
GTCCTAC42950.010.9333161
CTGTAGG45050.010.8888141
GTCCTAA24000.010.87445451
GGACCTG26500.010.8606876
GTATCAA58300.010.8157721
CCACCTT10900.010.71862713
TAATACT2600.010.5946154
TTATACT3150.010.5540234
TGGCGAG43000.010.44867118
AATCCCG3000.010.448482519
GCGCCAC1659.331416E-1010.36213113
GTAGGAC46850.010.3400143
TCCTACA48250.010.31562
Produced by FastQC (version 0.11.5)