##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512880_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2776886
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.897920188297253	31.0	30.0	34.0	23.0	34.0
2	29.057477692638443	31.0	30.0	34.0	22.0	34.0
3	29.268386602834973	31.0	30.0	34.0	23.0	34.0
4	32.665786784189194	35.0	33.0	37.0	25.0	37.0
5	32.90796201212437	35.0	35.0	37.0	26.0	37.0
6	32.802429411938405	37.0	35.0	37.0	25.0	37.0
7	32.71742844322741	37.0	33.0	37.0	25.0	37.0
8	32.67806348550138	37.0	35.0	37.0	25.0	37.0
9	33.98077918935095	39.0	34.0	39.0	23.0	39.0
10	33.82708400704962	39.0	34.0	39.0	22.0	39.0
11	33.91225819136976	39.0	34.0	39.0	23.0	39.0
12	33.80579361198119	39.0	34.0	39.0	22.0	39.0
13	33.786786349889766	39.0	34.0	39.0	22.0	39.0
14	34.665959639682725	39.0	34.0	41.0	18.0	41.0
15	34.84574159688226	39.0	34.0	41.0	22.0	41.0
16	34.80471866688082	39.0	35.0	41.0	21.0	41.0
17	34.59131775665259	39.0	34.0	41.0	18.0	41.0
18	34.58721928087793	39.0	34.0	41.0	18.0	41.0
19	34.697110720425684	40.0	34.0	41.0	18.0	41.0
20	34.66941674955328	40.0	34.0	41.0	18.0	41.0
21	34.560037754520714	39.0	34.0	41.0	18.0	41.0
22	34.591156784974245	40.0	34.0	41.0	18.0	41.0
23	34.26899015659988	39.0	34.0	41.0	11.0	41.0
24	34.34046878409845	39.0	34.0	41.0	11.0	41.0
25	34.07173935120131	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6849813114131074
1101	2	0.389162418975868
1101	3	0.7185834560420155
1101	4	0.8900662517376432
1101	5	0.7199024797502744
1101	6	0.8179101641791746
1101	7	0.4682304423576866
1101	8	0.8118768736716717
1101	9	0.8291518343081066
1101	10	0.8759779558489527
1101	11	0.8565089180179442
1101	12	0.7261734030011766
1101	13	1.1458358157735375
1101	14	1.2912927053492638
1101	15	1.1433489307379006
1101	16	0.9210851006886713
1101	17	0.3674990950758854
1101	18	0.8642506739095097
1101	19	0.35723131746457426
1101	20	-0.167229630214905
1101	21	0.831567956478203
1101	22	1.006487614021701
1101	23	0.46512146078968897
1101	24	0.976361697154779
1101	25	0.7736403515257493
1102	1	0.9379191611534488
1102	2	1.0201411627540686
1102	3	1.0342441802864428
1102	4	1.319409260937526
1102	5	1.0514981593476875
1102	6	0.9609445716872287
1102	7	1.0447576893182031
1102	8	1.0633783041425673
1102	9	1.3156508848695907
1102	10	1.3415258911302317
1102	11	1.1388868813025823
1102	12	1.240658583309191
1102	13	1.2476430205522604
1102	14	1.497714240785335
1102	15	1.4136405905723635
1102	16	1.3963034592010075
1102	17	1.5911555293105266
1102	18	1.5286259833229892
1102	19	1.3432972031887473
1102	20	1.5768183796454736
1102	21	1.6192097788210233
1102	22	1.4102291728068082
1102	23	1.4512771893567802
1102	24	0.7909047450078504
1102	25	1.1880425688987444
1103	1	0.8676481022359326
1103	2	0.8213354469481295
1103	3	0.9858219089486582
1103	4	1.0434013168007965
1103	5	0.9858809294543107
1103	6	0.611467338897782
1103	7	0.5750858716562703
1103	8	0.7470732024638238
1103	9	0.7888759805457326
1103	10	0.9955471280377566
1103	11	0.6957168581197593
1103	12	0.8619841378076813
1103	13	1.0588109934655492
1103	14	1.0874500350998062
1103	15	1.0431357632202065
1103	16	1.0910472210005508
1103	17	0.7145889568812791
1103	18	1.0643340952718248
1103	19	1.0799833585968557
1103	20	1.2446218804280775
1103	21	1.0674946785925101
1103	22	1.1698631869800238
1103	23	0.4550443294248083
1103	24	0.7335818221855206
1103	25	1.0487868022690492
1104	1	0.7794906857394679
1104	2	0.7633314870894878
1104	3	0.8494014402379513
1104	4	1.0199328274001758
1104	5	0.8065172637689031
1104	6	0.7379422839341458
1104	7	0.9032921375375693
1104	8	0.6996760529805002
1104	9	1.0432216211742897
1104	10	1.13090483185821
1104	11	0.8557494782226414
1104	12	1.1129895832863923
1104	13	1.0168977689699972
1104	14	1.2604436268488257
1104	15	1.0134278849107403
1104	16	1.06422464409858
1104	17	1.348779110865614
1104	18	1.1206323112892775
1104	19	0.8638261768703543
1104	20	0.8388830469440691
1104	21	1.0243218808936803
1104	22	0.854104737280295
1104	23	1.1715738046331552
1104	24	1.0212233909012411
1104	25	0.6096831712499053
1105	1	0.5606313983755591
1105	2	0.6382778744203996
1105	3	0.8016311773984022
1105	4	0.9158837490728047
1105	5	0.7453738652121089
1105	6	0.8712239080067619
1105	7	0.7875876438018992
1105	8	0.8905884412081377
1105	9	0.6121417118611276
1105	10	0.7077155019425518
1105	11	0.6993316081789018
1105	12	0.8727075404639351
1105	13	0.8361983298409967
1105	14	0.9714842413536431
1105	15	0.9200779707756084
1105	16	0.9521981698030544
1105	17	1.1811887624149904
1105	18	1.074317627128174
1105	19	0.8179619660234678
1105	20	1.0065596832407948
1105	21	0.8564357639313513
1105	22	0.9237300407681843
1105	23	1.095468241604955
1105	24	1.1647416509322
1105	25	1.4145892063233774
1106	1	0.6065970068411701
1106	2	0.72847193376003
1106	3	0.6359775038333382
1106	4	0.7187825321931101
1106	5	0.5998325804303164
1106	6	0.7060058923140673
1106	7	0.6283484125689967
1106	8	0.6029631211461037
1106	9	0.7945802882870439
1106	10	0.564130974655491
1106	11	0.393001979064465
1106	12	0.63234288870688
1106	13	0.6262994617142752
1106	14	0.9953066449228842
1106	15	0.7128761243332562
1106	16	0.7927341867631199
1106	17	0.9215344165264625
1106	18	0.8730156766173565
1106	19	0.7798169476569257
1106	20	0.8252110564807751
1106	21	0.801229249864079
1106	22	0.8747582963850462
1106	23	1.1543103898458398
1106	24	0.7597260149039116
1106	25	0.9671048165921476
1107	1	0.9623207611710392
1107	2	0.8957290563320157
1107	3	0.8910881832223154
1107	4	1.0162564728717882
1107	5	0.8829160292830522
1107	6	0.9967608410976538
1107	7	0.8554594452618289
1107	8	0.869668638588692
1107	9	0.9364147110441934
1107	10	1.215567736638313
1107	11	1.0450745193444746
1107	12	1.1218692001471737
1107	13	0.858808812883133
1107	14	1.1562773409399085
1107	15	0.9179220032419408
1107	16	1.0123791459012992
1107	17	1.2997678002835968
1107	18	1.2519527168585824
1107	19	1.0766508940019648
1107	20	1.2763982080253626
1107	21	1.0300693845074207
1107	22	1.1021911180601975
1107	23	1.380853944032026
1107	24	1.206041852529168
1107	25	1.4841500411719792
1108	1	0.9971091647951873
1108	2	0.9032120681336657
1108	3	0.9580435634619526
1108	4	1.0387807164465173
1108	5	0.8968774004365727
1108	6	0.9641140604899405
1108	7	1.0000791251887264
1108	8	0.9799966105581674
1108	9	1.107552309015908
1108	10	1.2071351714203615
1108	11	0.9539210110241214
1108	12	1.0115609351312855
1108	13	1.077062117216407
1108	14	1.254142474593273
1108	15	1.1960916857428074
1108	16	1.0616996179672924
1108	17	1.2292816578916046
1108	18	1.3512311206114305
1108	19	1.2244116210196339
1108	20	1.2581728956098246
1108	21	1.24432016635064
1108	22	1.23093785233646
1108	23	1.462466069358598
1108	24	1.3525131817269909
1108	25	1.5134104432260074
1109	1	0.7300980863960618
1109	2	0.7690994631455759
1109	3	0.8576155891003054
1109	4	0.8578179546280893
1109	5	0.8383459902470136
1109	6	0.865819540326612
1109	7	0.7204526300861644
1109	8	0.7129855321590384
1109	9	0.7874603767537778
1109	10	0.9353311411613277
1109	11	0.9139491281775918
1109	12	0.8436967157132003
1109	13	0.9074667475219655
1109	14	1.0028751971840606
1109	15	0.8200492252311662
1109	16	0.49932546328685135
1109	17	0.992344702887074
1109	18	1.004752382955303
1109	19	0.9816717427937789
1109	20	0.882059239738112
1109	21	0.8456668616826306
1109	22	0.9011547274417495
1109	23	1.1014783189715374
1109	24	0.9497660435799133
1109	25	0.6244922517084817
1110	1	0.7371220920338146
1110	2	0.7308236066152247
1110	3	0.6151485588304944
1110	4	0.7282167736707734
1110	5	0.615736992123253
1110	6	0.6059324359909795
1110	7	0.514449865417312
1110	8	0.5500408787414983
1110	9	0.7447985949377554
1110	10	0.6038923187456291
1110	11	0.6036789957364945
1110	12	0.5531553666471538
1110	13	0.6965872133997877
1110	14	0.6530071062210325
1110	15	0.6356158468238959
1110	16	0.7103462926674311
1110	17	0.7509461960491848
1110	18	0.19337954517430944
1110	19	0.6295474451290914
1110	20	0.7338401121246463
1110	21	0.8808031024356993
1110	22	0.8204243890659555
1110	23	0.8512611810362429
1110	24	0.7905353222262121
1110	25	0.9347432603508352
1111	1	0.2120348861920931
1111	2	0.22543148168270477
1111	3	0.34637158192141726
1111	4	0.13919359140438559
1111	5	0.18009276949622688
1111	6	0.037155983177967755
1111	7	0.03049057420482626
1111	8	0.2105856541401323
1111	9	0.22605714425481693
1111	10	0.13337444444931634
1111	11	-0.05089932031289379
1111	12	-0.28680830600843166
1111	13	0.14607251362821927
1111	14	0.12052071212924176
1111	15	0.02843917478898561
1111	16	0.21092766494559356
1111	17	-0.35669744557991834
1111	18	0.09712181569322809
1111	19	0.051986094069334854
1111	20	0.22662458211004122
1111	21	0.052828501628439994
1111	22	-0.26895390572011024
1111	23	0.15170928317208876
1111	24	0.11695537923288413
1111	25	0.21975523594592516
1112	1	0.29239688062510893
1112	2	0.2817376316359663
1112	3	0.25697975852149213
1112	4	0.43712500597184345
1112	5	0.40695253143781684
1112	6	0.03346010527560139
1112	7	-0.028406050303658503
1112	8	0.12475323543876016
1112	9	-5.344572157994776E-4
1112	10	0.04405633434168976
1112	11	-0.05670253986675533
1112	12	0.01411861150545235
1112	13	0.034899053768540966
1112	14	-0.01725520390273516
1112	15	-0.1879161963024245
1112	16	0.011833687158748774
1112	17	0.23786992424939513
1112	18	-0.004565276314920652
1112	19	0.10722369732160075
1112	20	-0.14770700879697785
1112	21	-0.0020155693484795734
1112	22	-0.16667705803402555
1112	23	0.11123150854381691
1112	24	0.03778380019696925
1112	25	0.07878315300515482
1113	1	-0.3340625704463136
1113	2	-0.21284338803807046
1113	3	-0.2530476708850955
1113	4	-0.1492783175427448
1113	5	-0.32382921852585866
1113	6	-0.5895532024796495
1113	7	-0.6027831288363146
1113	8	-0.4886974860429092
1113	9	-0.584978996887493
1113	10	-0.5017199269696206
1113	11	-0.591919776095736
1113	12	-0.6483227857989533
1113	13	-0.5103707987048764
1113	14	-0.31070546816165034
1113	15	-0.6422356845613066
1113	16	-0.7041769473278805
1113	17	-0.5191530469384489
1113	18	-0.7435810439681774
1113	19	-0.9124398995528153
1113	20	-0.7371312188803429
1113	21	-0.4553667746153707
1113	22	-1.0233108059819642
1113	23	-4.656049136365908
1113	24	-2.2351433477900073
1113	25	-1.3269294494394686
1114	1	-0.7719942414569196
1114	2	-0.9574352479621027
1114	3	-0.8449292382988212
1114	4	-0.7659800575874165
1114	5	-0.7501040553472436
1114	6	-1.3596892477842424
1114	7	-1.2329235361148179
1114	8	-1.0577201042095332
1114	9	-1.3007411436113046
1114	10	-1.1006979223096351
1114	11	-1.2541947785722414
1114	12	-1.9719846837071167
1114	13	-1.2768775193912063
1114	14	-3.16662412151042
1114	15	-1.9580883797034474
1114	16	-1.9085741333552448
1114	17	-1.8477804361388976
1114	18	-1.7958791549171451
1114	19	-1.7341709580557136
1114	20	-1.4176693857358345
1114	21	-1.2537973796894093
1114	22	-1.3804103763608353
1114	23	-5.619799593166135
1114	24	-2.820394548373379
1114	25	-2.5767104142514903
1115	1	-1.5274878380830685
1115	2	-1.5154277089365387
1115	3	-1.2424982721342168
1115	4	-1.2958140458619347
1115	5	-1.272665156792545
1115	6	-1.3834594043847446
1115	7	-1.7929371547086888
1115	8	-2.1229766866580526
1115	9	-3.3744394806850373
1115	10	-3.942503509603295
1115	11	-2.8438061309271134
1115	12	-3.054235751978368
1115	13	-2.3279190024851957
1115	14	-3.0982169321148803
1115	15	-2.6826810239199403
1115	16	-2.8372567435365283
1115	17	-2.713501244097557
1115	18	-4.840959222591838
1115	19	-3.1654405782267325
1115	20	-3.5469368450161447
1115	21	-3.2394578225438586
1115	22	-2.8401719115425585
1115	23	-3.1764896556421007
1115	24	-3.0237462766502397
1115	25	-3.1985207092072265
1116	1	-1.8878370339411852
1116	2	-1.6860600067995044
1116	3	-2.2396342051591134
1116	4	-2.082840373246821
1116	5	-2.170109527799376
1116	6	-4.367555570941253
1116	7	-3.014255176299347
1116	8	-3.4578522099010556
1116	9	-4.375554814458997
1116	10	-3.8100541815574687
1116	11	-3.337334441127595
1116	12	-3.138464106241422
1116	13	-4.303849824252104
1116	14	-7.421622417904704
1116	15	-5.2942694795269
1116	16	-4.261524203329433
1116	17	-5.1410320878957485
1116	18	-4.06916202056496
1116	19	-3.772622776948918
1116	20	-4.414996990422619
1116	21	-5.163739051889529
1116	22	-4.8710157097359925
1116	23	-3.9052983139368784
1116	24	-4.7601281889657265
1116	25	-4.6774257543713595
1201	1	0.35136705161121284
1201	2	0.5194591931017207
1201	3	0.8672294679966939
1201	4	0.779879475568535
1201	5	0.2809050007432461
1201	6	0.780671404954262
1201	7	0.8771328208470734
1201	8	0.906354619474314
1201	9	1.407105081824497
1201	10	1.221467028194077
1201	11	1.26770767655497
1201	12	1.2731959409523341
1201	13	1.2600078536505208
1201	14	1.4318017016711053
1201	15	1.4106762613739008
1201	16	1.3673538474587303
1201	17	1.5583102520364776
1201	18	1.3330935734041134
1201	19	1.5971896827511856
1201	20	1.4821854463665574
1201	21	1.6849461424417314
1201	22	1.4476464021845459
1201	23	1.8687130523497686
1201	24	1.683117719308271
1201	25	1.7396044688386354
1202	1	1.01609557277704
1202	2	1.0229513853975547
1202	3	1.080685246880428
1202	4	1.3256170220732812
1202	5	1.1197440806276333
1202	6	1.3628101857504404
1202	7	1.3540433101287022
1202	8	1.3309777440255104
1202	9	1.614113178019835
1202	10	1.4746761595619233
1202	11	1.4705905977308689
1202	12	1.631556140118093
1202	13	1.5544056799112056
1202	14	1.9845774376163448
1202	15	1.7037245168292472
1202	16	1.5767278347756601
1202	17	1.8194002422561724
1202	18	1.7364136077478847
1202	19	1.8397201616758139
1202	20	1.712569553854479
1202	21	1.8333852309911052
1202	22	1.755998712119208
1202	23	1.9205373675162107
1202	24	1.900684621890754
1202	25	2.214738754090405
1203	1	1.0188631894100908
1203	2	1.0774855012481623
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2211	16	0.03901928630293838
2211	17	0.46333596809571276
2211	18	0.15795152116709232
2211	19	0.1117274417023495
2211	20	0.058720394968545975
2211	21	0.2486866694504144
2211	22	0.2968409847590152
2211	23	0.5306507267176102
2211	24	0.1600784835595377
2211	25	0.545742387406726
2212	1	-0.025019038269686433
2212	2	0.10860453449619456
2212	3	-0.23727556176785214
2212	4	0.02417351294215564
2212	5	-0.20886774093261096
2212	6	-0.15153218538081603
2212	7	-0.09998586976642798
2212	8	-0.036883610961147895
2212	9	-0.002512520872691937
2212	10	-0.2097948279812769
2212	11	-0.2796549185584922
2212	12	0.06966559970572916
2212	13	0.04224545457324069
2212	14	0.10917261987246718
2212	15	-0.22012254381937169
2212	16	-0.2662291785118356
2212	17	-0.07771984627735407
2212	18	0.12023461383235201
2212	19	-0.19152302706878999
2212	20	0.00988442942886536
2212	21	0.1179621844543135
2212	22	0.19796812966890087
2212	23	0.20610822157885167
2212	24	0.12064935337969729
2212	25	0.26214567234870145
2213	1	-0.34349343046971725
2213	2	-0.2214037777931992
2213	3	-0.5670170806268509
2213	4	-0.2958163585937541
2213	5	-0.2727656097963802
2213	6	-0.06257505394714968
2213	7	-0.34554074272105595
2213	8	-0.1096931595127657
2213	9	-0.5582567129302731
2213	10	-0.3127974648563594
2213	11	-0.36569548400473906
2213	12	-0.522797380600089
2213	13	-0.4947008029750748
2213	14	-0.5552802614491981
2213	15	-0.4160991641235725
2213	16	-0.4889428909466318
2213	17	-0.396469402104195
2213	18	-0.4427473765582377
2213	19	-0.43799114635935865
2213	20	-0.18032591518633723
2213	21	-0.37342356736567694
2213	22	-0.5656722635302884
2213	23	-0.3968539330255254
2213	24	-0.19800981485440872
2213	25	0.021326119569870627
2214	1	-2.717782980405268
2214	2	-1.6035497198094752
2214	3	-1.7541893592853235
2214	4	-3.542853021756798
2214	5	-1.9006391879670694
2214	6	-1.6402848736567428
2214	7	-1.509098501214865
2214	8	-1.369493021845642
2214	9	-1.2304759039649582
2214	10	-1.2358770754040975
2214	11	-1.2888620656825225
2214	12	-1.2358283319155845
2214	13	-1.5837644435419733
2214	14	-1.4693230512451834
2214	15	-1.8778184867299714
2214	16	-1.7140650593673428
2214	17	-3.6034807618718006
2214	18	-3.439899629556514
2214	19	-2.287684220038031
2214	20	-2.1284651046748735
2214	21	-2.0339276728490177
2214	22	-2.9248496504418
2214	23	-2.1225915894434735
2214	24	-3.171255413892222
2214	25	-3.086017068509282
2215	1	-1.941429041184879
2215	2	-3.668699586893794
2215	3	-2.1937059684489455
2215	4	-3.789959302433509
2215	5	-2.3444818572966746
2215	6	-2.035121872063506
2215	7	-2.1984845323212987
2215	8	-1.7193063408805251
2215	9	-4.381008845064944
2215	10	-2.510779863236486
2215	11	-2.038090833325171
2215	12	-2.025401153563127
2215	13	-2.165932192697692
2215	14	-1.8014095315401306
2215	15	-1.9351212517705534
2215	16	-2.034574836102607
2215	17	-2.3226097350059547
2215	18	-2.0016102715038073
2215	19	-2.00875086196487
2215	20	-1.9852686414956935
2215	21	-2.3937511240106346
2215	22	-2.438042965137477
2215	23	-2.4099842141026
2215	24	-2.2284518902205193
2215	25	-3.074379732900667
2216	1	-3.4745906353056846
2216	2	-3.6526190648124874
2216	3	-4.882929984583196
2216	4	-5.655208137152428
2216	5	-5.3583239460159575
2216	6	-4.227054615725592
2216	7	-5.876122534783896
2216	8	-3.8784131850604133
2216	9	-4.732842509803959
2216	10	-3.5795908809767845
2216	11	-3.928880908424471
2216	12	-4.331423230901262
2216	13	-3.936197136637844
2216	14	-4.893446808472074
2216	15	-4.187658010559655
2216	16	-4.557303207722754
2216	17	-4.290989300445503
2216	18	-3.8935573094547458
2216	19	-4.373194059707863
2216	20	-5.090188621121808
2216	21	-4.746191140017828
2216	22	-4.442196903570906
2216	23	-4.580004677452678
2216	24	-4.895965123243869
2216	25	-4.932760021096449
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	132699.0
3	5.0
4	1138.0
5	7578.0
6	9051.0
7	8063.0
8	7280.0
9	6543.0
10	5999.0
11	5530.0
12	5260.0
13	4996.0
14	5135.0
15	5035.0
16	5391.0
17	5694.0
18	6455.0
19	8000.0
20	10183.0
21	13026.0
22	16624.0
23	19590.0
24	22919.0
25	26500.0
26	28904.0
27	32057.0
28	35906.0
29	42216.0
30	51108.0
31	63583.0
32	79426.0
33	102171.0
34	132619.0
35	175467.0
36	241697.0
37	381873.0
38	1071165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.51103199297838	18.781362343904192	12.379731384584588	24.327874278532846
2	14.480468802053151	20.02492968941612	42.95181640627111	22.54278510225963
3	19.476732774358567	25.934198674580816	28.64384932675606	25.945219224304562
4	12.036613189896258	16.43747107518381	38.49661185732885	33.029303877591076
5	12.557008800505665	39.35682655962716	35.13481683170991	12.95134780815726
6	31.17677630862851	36.91771554697597	18.708934723014142	13.196573421381371
7	28.399024126170147	31.833108130756138	21.785348845339712	17.982518897734
8	25.263142715515297	37.58547158758055	20.480050470911632	16.67133522599252
9	27.933800771550594	14.686205694682084	20.069182201784667	37.310811331982656
10	16.12966829919938	28.932235654240458	33.70626739004195	21.231828656518218
11	34.26091463652	22.235674832429098	25.317732310438345	18.185678220612555
12	24.725553803999293	24.705979471824676	31.42768978540692	19.14077693876911
13	29.72306073128403	22.501085806659734	25.658124987610094	22.11772847444615
14	20.928750520850606	23.294061077573218	27.474779296314612	28.302409105261567
15	22.775625367420595	32.67722963701459	23.19290342555838	21.35424157000644
16	21.244327500135167	28.154450592030567	29.225584371113953	21.375637536720312
17	21.04325695159165	29.342088424498154	28.151823807094793	21.462830816815398
18	20.742529355526685	26.924962616135122	31.81150601097939	20.521002017358803
19	23.648667646679264	26.436044084705962	28.001084310161733	21.91420395845304
20	24.17422662091583	27.03614106916083	28.736333235646	20.05329907427734
21	22.931818099903044	26.636210618888516	27.312082580942803	23.119888700265633
22	23.48466960088798	28.91562269168001	27.36482820601651	20.2348795014155
23	21.632362769391154	28.428268939478595	28.692460255950884	21.246908035179366
24	23.270368159548298	26.620628700123135	29.463361391549086	20.645641748779482
25	22.194354971264453	27.681035402542804	29.13832281282531	20.98628681336744
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	416.0
1	416.0
2	954.5
3	1493.0
4	1493.0
5	1493.0
6	2745.5
7	3998.0
8	3998.0
9	3998.0
10	4777.0
11	5556.0
12	5556.0
13	5556.0
14	7804.5
15	10053.0
16	10053.0
17	10053.0
18	19107.5
19	28162.0
20	28162.0
21	28162.0
22	48069.5
23	67977.0
24	67977.0
25	67977.0
26	105028.0
27	142079.0
28	142079.0
29	142079.0
30	187299.0
31	232519.0
32	232519.0
33	232519.0
34	258206.5
35	283894.0
36	283894.0
37	283894.0
38	299098.0
39	314302.0
40	314302.0
41	314302.0
42	327919.0
43	341536.0
44	341536.0
45	341536.0
46	357120.5
47	372705.0
48	372705.0
49	372705.0
50	353359.0
51	334013.0
52	334013.0
53	334013.0
54	289221.5
55	244430.0
56	244430.0
57	244430.0
58	208408.5
59	172387.0
60	172387.0
61	172387.0
62	142507.5
63	112628.0
64	112628.0
65	112628.0
66	86394.0
67	60160.0
68	60160.0
69	60160.0
70	44769.0
71	29378.0
72	29378.0
73	29378.0
74	20813.0
75	12248.0
76	12248.0
77	12248.0
78	8551.5
79	4855.0
80	4855.0
81	4855.0
82	3168.0
83	1481.0
84	1481.0
85	1481.0
86	969.5
87	458.0
88	458.0
89	458.0
90	286.5
91	115.0
92	115.0
93	115.0
94	72.0
95	29.0
96	29.0
97	29.0
98	21.5
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3397690794652715
2	0.009975202438991014
3	0.009182948093655987
4	0.010479364295113303
5	0.01227994235269291
6	0.08426705309472554
7	0.08451913402278667
8	0.10843081062744385
9	0.08905659072788728
10	0.09611485671359933
11	0.09139734220274078
12	0.10245289147627955
13	0.08826433638255225
14	0.09283780464880445
15	0.08920063697249365
16	0.09132531908043758
17	0.10374930767773685
18	0.10666624413101583
19	0.09982404751221331
20	0.09827555038269485
21	0.08628370051921469
22	0.10634214008065149
23	0.09939190877839423
24	0.09557468329632546
25	0.0944223133394745
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2776886.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.98362272031226
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.31029467875105	37.376244054163585
2	14.444071021707797	14.728221350322912
3	5.256493418421459	8.039852318298125
4	2.4753744878505572	5.04814235920236
5	1.3300501407565557	3.390538728771523
6	0.8040048042733181	2.459464656383361
7	0.5047474179659563	1.8013696348639658
8	0.35089881971552145	1.4312074429903212
9	0.25309045736924585	1.1613121553362418
>10	1.1444215302500316	10.029903799742797
>50	0.06836111126547437	2.4164889461536836
>100	0.048511039278138574	4.856967251546253
>500	0.005552379755998372	2.0165488810916026
>1k	0.003630402148126636	3.1851992755752234
>5k	3.5592177922810157E-4	1.2876893465126702
>10k+	1.423687116912406E-4	0.7708497990453796
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11210	0.4036896005093475	No Hit
GTCCTACAGTGGACATTTCTAAATT	10030	0.3611959583504688	No Hit
CTGTAGGACGTGGAATATGGCAAGA	8347	0.3005885009323393	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	8056	0.290109136637226	No Hit
CTTTAGGACGTGAAATATGGCGAGG	6634	0.23890069667966204	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6254	0.22521630344205704	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6190	0.22291156352835512	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4467	0.16086364366416195	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3350	0.12063872985783357	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3069	0.11051948117423618	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3051	0.10987127307350752	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2922	0.10522578168495214	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2864	0.10313711113815978	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2816	0.10140855620288337	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2808	0.10112046371367064	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	3.601156115159211E-5
9	0.0	0.0	0.0	0.0	7.202312230318422E-5
10	0.0	0.0	0.0	0.0	7.202312230318422E-5
11	0.0	0.0	0.0	0.0	7.202312230318422E-5
12	0.0	0.0	0.0	3.601156115159211E-5	7.202312230318422E-5
13	0.0	0.0	0.0	7.202312230318422E-5	7.202312230318422E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1550	0.0	13.35935	4
GGTATCA	2645	0.0	12.856143	1
TACGCTG	55	0.003072829	12.089154	5
GGACCGT	90	7.478433E-6	11.609425	6
AGGACCT	2755	0.0	11.377657	5
GGCGAGG	1940	0.0	11.016442	19
TGTAGGA	4655	0.0	10.998403	2
GTCCTAC	4295	0.0	10.933316	1
CTGTAGG	4505	0.0	10.888814	1
GTCCTAA	2400	0.0	10.8744545	1
GGACCTG	2650	0.0	10.860687	6
GTATCAA	5830	0.0	10.815772	1
CCACCTT	1090	0.0	10.718627	13
TAATACT	260	0.0	10.594615	4
TTATACT	315	0.0	10.554023	4
TGGCGAG	4300	0.0	10.448671	18
AATCCCG	300	0.0	10.4484825	19
GCGCCAC	165	9.331416E-10	10.362131	13
GTAGGAC	4685	0.0	10.340014	3
TCCTACA	4825	0.0	10.3156	2
>>END_MODULE