FastQCFastQC Report
Thu 26 May 2016
SRR1512880_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512880_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2776886
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT128740.46361283826559674No Hit
GTCCTACAGTGGACATTTCTAAATT93980.3384366517026626No Hit
TATCAACGCAGAGTACTTTTTTTTT88900.32014277863765384No Hit
CTGTAGGACGTGGAATATGGCAAGA82810.2982117378963342No Hit
GTCCTAAAGTGTGTATTTCTCATTT76600.27584855842119554No Hit
GGTATCAACGCAGAGTACTTTTTTT72620.2615159570828619No Hit
CTTTAGGACGTGAAATATGGCGAGG70010.25211693962229637No Hit
GTCCTACAGTGTGCATTTCTCATTT45290.16309636045556067No Hit
ACGCAGAGTACTTTTTTTTTTTTTT45080.1623401176713772No Hit
CTGAAGGACCTGGAATATGGCGAGA36260.13057792073567298No Hit
CTGTAGGACCTGGAATATGGCGAGA30330.10922306497277885No Hit
TTTCTAAATTTTCCACCTTTTTCAG29470.10612607071374194No Hit
ATTTAGAAATGTCCACTGTAGGACG28370.10216479898706682No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21450.013.1267021
TGTCGAG1800.012.65628218
TAGGACC15400.012.5354864
GGACCGT1001.4253055E-712.3623596
CCAACGA1603.6379788E-1211.86483819
GGCGAGG20050.011.78790919
ATGTCGA2100.011.75226317
TCCAACG2750.011.73582518
CCGCCCA658.038475E-411.69039
TATAGCG658.0823223E-411.68272216
GTATCAA52450.011.4076561
CGATACT600.005833248611.0946254
CGTTTTA600.00591362111.07424617
CCGTACA2000.010.91604317
TGTAGGA44450.010.76337052
CCCGTAC2050.010.64979916
CTGTAGG44850.010.5835011
GACGTGG21500.010.5222477
TATGTCG2350.010.50202116
GCCGGTT1553.7071004E-910.412511
Produced by FastQC (version 0.11.5)