##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512880_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2776886 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.30180965297099 31.0 30.0 34.0 26.0 34.0 2 30.320243250893267 31.0 30.0 34.0 26.0 34.0 3 30.48925054899625 31.0 31.0 34.0 26.0 34.0 4 34.066469059226776 37.0 35.0 37.0 30.0 37.0 5 33.64971194352235 37.0 35.0 37.0 28.0 37.0 6 33.499846230633885 37.0 35.0 37.0 28.0 37.0 7 33.34412683848023 37.0 35.0 37.0 27.0 37.0 8 33.289980215248306 37.0 35.0 37.0 27.0 37.0 9 34.61268809738679 39.0 35.0 39.0 27.0 39.0 10 34.533968625287464 39.0 35.0 39.0 27.0 39.0 11 34.501126081517214 39.0 35.0 39.0 27.0 39.0 12 34.36940947521792 39.0 35.0 39.0 26.0 39.0 13 34.36341859190475 39.0 35.0 39.0 26.0 39.0 14 35.45862091565876 40.0 36.0 41.0 26.0 41.0 15 35.46396467121805 40.0 36.0 41.0 26.0 41.0 16 35.46933903660431 40.0 36.0 41.0 26.0 41.0 17 35.47554058755023 40.0 36.0 41.0 26.0 41.0 18 35.40252750743099 40.0 36.0 41.0 25.0 41.0 19 35.41691052495493 40.0 36.0 41.0 25.0 41.0 20 35.392796463376605 40.0 36.0 41.0 25.0 41.0 21 35.36188738032458 40.0 36.0 41.0 25.0 41.0 22 35.32756188046611 40.0 36.0 41.0 25.0 41.0 23 35.174970452514074 40.0 36.0 41.0 25.0 41.0 24 35.13585685548488 40.0 36.0 41.0 24.0 41.0 25 35.079783973846965 40.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.547237059337661 1101 2 0.5134658542784649 1101 3 0.399380491508623 1101 4 0.40267271886852996 1101 5 0.5668050289124764 1101 6 0.5052957345887208 1101 7 0.630050756832695 1101 8 0.3702418282850317 1101 9 0.6847558265165787 1101 10 0.3975641996235382 1101 11 0.7091552553326537 1101 12 0.76263677159087 1101 13 0.8429314223446553 1101 14 0.861873727930849 1101 15 0.5596990889495075 1101 16 0.8038339270470871 1101 17 0.8395198583207488 1101 18 0.6398983496667014 1101 19 0.5132611486298657 1101 20 0.6769278921186626 1101 21 0.7125052042838789 1101 22 0.5843909455860512 1101 23 0.8168783573498786 1101 24 0.7770356921175008 1101 25 0.8976973153347529 1102 1 0.7936679527952712 1102 2 0.8963942326415264 1102 3 0.5406114203870978 1102 4 0.6451088325264038 1102 5 0.9279560857639737 1102 6 0.8810021737447968 1102 7 0.9216833746892732 1102 8 0.9195510709418571 1102 9 1.0921494042740747 1102 10 1.05317755912521 1102 11 1.202971306797508 1102 12 1.1116633095168353 1102 13 1.118997042075641 1102 14 1.1795456748426503 1102 15 1.2374518910529417 1102 16 1.2379496057960182 1102 17 1.3096357494152855 1102 18 0.6684305510587762 1102 19 1.0336674829487222 1102 20 1.1383127536052413 1102 21 1.108705575933591 1102 22 0.9054684469067595 1102 23 1.1573639890606628 1102 24 1.2135850400806802 1102 25 1.1517016630325045 1103 1 0.6299592362010777 1103 2 0.8495231462979262 1103 3 0.5605966155644495 1103 4 0.3893374345098266 1103 5 0.6783557206090407 1103 6 0.6603095237703371 1103 7 0.8069989987976101 1103 8 0.7269545345863477 1103 9 0.9243857491380894 1103 10 0.7364738365552697 1103 11 0.9784464592824662 1103 12 1.0923743443932281 1103 13 1.1090802991980127 1103 14 1.1528960075236512 1103 15 1.1177852198919922 1103 16 1.0153869508460573 1103 17 1.0969128316572494 1103 18 1.0912590701939493 1103 19 1.171531337554093 1103 20 0.9808112433620622 1103 21 1.0519554548346122 1103 22 1.06187850432066 1103 23 1.1794609487008074 1103 24 1.148748993132756 1103 25 1.093846496641575 1104 1 0.5597177839011138 1104 2 0.675508143635227 1104 3 0.6649100291682899 1104 4 0.4194059754952235 1104 5 0.5599208932376598 1104 6 0.5187794158676908 1104 7 0.5439797923524239 1104 8 0.6330678154051554 1104 9 0.661790367106498 1104 10 0.6103731234684062 1104 11 0.6999094962043557 1104 12 0.6314092136570508 1104 13 0.5501583672134558 1104 14 0.8175144497818181 1104 15 0.8873758316777653 1104 16 0.7736248729810384 1104 17 0.7753169558548763 1104 18 0.3613693528494579 1104 19 0.8188252097886135 1104 20 0.8005772057008684 1104 21 0.6727273741922062 1104 22 0.6576891526465545 1104 23 0.7477685641325138 1104 24 0.9428214821306042 1104 25 0.804161567635262 1105 1 0.5785795033979362 1105 2 0.5369264789565626 1105 3 0.6014675247904506 1105 4 0.4158414203429146 1105 5 0.489794781858663 1105 6 0.6449360536871396 1105 7 0.6233352136824379 1105 8 0.6440911934897358 1105 9 0.7889941967641363 1105 10 0.5571805789074986 1105 11 0.7315000476852092 1105 12 0.7126820016850317 1105 13 0.8414999740285083 1105 14 0.9412460395930822 1105 15 0.9432110472704878 1105 16 0.8509786489202682 1105 17 0.6298279279466783 1105 18 0.5245746713954276 1105 19 0.8261581962786408 1105 20 0.9103840943472505 1105 21 0.7935319449101712 1105 22 0.7546382005193877 1105 23 0.9911958835413586 1105 24 0.9293338246764336 1105 25 0.8980906466393961 1106 1 -0.007884255534175821 1106 2 0.33925565470346086 1106 3 -1.6582727934941701 1106 4 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0.7649870026768184 1107 17 0.737638657385105 1107 18 0.8108909480444311 1107 19 0.8163616379209344 1107 20 0.8075531722717244 1107 21 0.7005552273830915 1107 22 0.7600643541020133 1107 23 1.046579873053048 1107 24 0.9882004676258092 1107 25 0.8990788670520828 1108 1 0.7105221731313058 1108 2 0.6546643612293295 1108 3 0.6197718948390083 1108 4 0.5206150381979242 1108 5 0.6750692941680612 1108 6 0.7820750774540741 1108 7 0.6483053985363441 1108 8 0.5608483704213256 1108 9 0.8155243526547622 1108 10 0.7463949498079145 1108 11 0.7010406814700261 1108 12 0.87386481112366 1108 13 0.8065630117659595 1108 14 1.0612253819561488 1108 15 0.97553918424979 1108 16 0.8297939227936766 1108 17 0.9065284561006948 1108 18 0.9956380072843416 1108 19 1.0135357412143264 1108 20 0.9805295496671249 1108 21 1.0164906722293594 1108 22 1.1154334130587884 1108 23 1.1061226234680959 1108 24 1.258871051015987 1108 25 1.1995696908509998 1109 1 0.30232126985270824 1109 2 0.3257075036120298 1109 3 0.40102525405670164 1109 4 0.38072237272386644 1109 5 0.12933078770632989 1109 6 0.2718640584178402 1109 7 0.33532498026861646 1109 8 0.43942558630170225 1109 9 0.5835594409370088 1109 10 0.19496803435190202 1109 11 0.4785168761482197 1109 12 0.6541844080977555 1109 13 0.5494468851144276 1109 14 0.7095592099802488 1109 15 0.5996455320682017 1109 16 0.671468678322853 1109 17 0.748431838983322 1109 18 0.8369210880579843 1109 19 0.7694740358949304 1109 20 0.8524579211183863 1109 21 0.7906744324674548 1109 22 0.8010072276059006 1109 23 0.75378561070508 1109 24 0.7635859888052821 1109 25 0.7651993787144988 1110 1 0.3192476387101273 1110 2 0.14692729077067312 1110 3 0.2272442832905135 1110 4 0.256892061553053 1110 5 0.24898227278725926 1110 6 0.3834722544463105 1110 7 0.3653514524246688 1110 8 0.3603460182485847 1110 9 0.26420695971196295 1110 10 0.46354627779334834 1110 11 0.4261492394381605 1110 12 0.5853812466979136 1110 13 0.518885046981076 1110 14 0.5585920778172309 1110 15 0.5954849262905029 1110 16 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2215 9 -1.4011409232179162 2215 10 -1.3664243026055445 2215 11 -1.1696128700093453 2215 12 -1.448966723739204 2215 13 -1.5046164945369398 2215 14 -1.7685310030921215 2215 15 -1.5443141826016813 2215 16 -1.5881939981605413 2215 17 -1.7457934744604415 2215 18 -1.3991703760741103 2215 19 -1.725880751168468 2215 20 -1.5989275973624046 2215 21 -1.2501017173459417 2215 22 -1.5227969493647393 2215 23 -1.9598394756572688 2215 24 -1.8637017961653086 2215 25 -2.0823281692386004 2216 1 -1.7450239251472226 2216 2 -1.1398499793080177 2216 3 -1.1132719602874452 2216 4 -1.4883722036992637 2216 5 -2.1296508871443756 2216 6 -2.592222910163887 2216 7 -2.4675418794918293 2216 8 -2.569202730948298 2216 9 -4.436485864411797 2216 10 -2.61509542127639 2216 11 -2.6241229543687687 2216 12 -2.9289841724217496 2216 13 -3.0352377361919487 2216 14 -3.0448987639822462 2216 15 -3.0747532273611995 2216 16 -2.3732037221973385 2216 17 -2.745404703981386 2216 18 -3.1159108982418715 2216 19 -3.276789076827754 2216 20 -2.6120297405105575 2216 21 -3.307119896524952 2216 22 -2.7091483864182564 2216 23 -3.7354325604783902 2216 24 -3.0608979945278207 2216 25 -3.1970931643949285 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78269.0 3 1.0 4 1304.0 5 11635.0 6 14461.0 7 11302.0 8 9001.0 9 7557.0 10 6429.0 11 5694.0 12 5078.0 13 4671.0 14 4405.0 15 4558.0 16 4623.0 17 4922.0 18 5244.0 19 6471.0 20 8715.0 21 11477.0 22 14469.0 23 17337.0 24 20179.0 25 23209.0 26 25187.0 27 27674.0 28 31138.0 29 37006.0 30 46544.0 31 58442.0 32 75152.0 33 97036.0 34 126228.0 35 166910.0 36 231869.0 37 362677.0 38 1210012.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.790920172692324 19.398078296894408 13.374680202738306 26.43632132767496 2 12.979239974766463 20.402562119213698 41.86475990850501 24.753437997514833 3 17.560539518579763 25.75053298836917 29.384470921472865 27.304456571578207 4 11.227691336985599 16.89037153134413 37.77408197573832 34.10785515593196 5 12.231600588683191 39.464969676188225 35.15721630361415 13.146213431514434 6 31.29015740640313 37.21706718824376 18.288661643796623 13.204113761556489 7 28.028031455199404 31.937262590368825 21.792095836006258 18.24261011842551 8 25.239560667624367 37.8609083470341 20.149614847338736 16.7499161380028 9 27.310498792981353 14.520639622629275 20.034996696997617 38.13386488739175 10 15.579740561644442 29.373504975937298 33.644837588908786 21.40191687350948 11 34.00788195861234 22.219589220523055 25.307980167975103 18.464548652889505 12 24.31164627626915 24.89715972500062 31.444978337190783 19.34621566153945 13 29.3741703274897 22.412412152852056 25.833706078226726 22.379711441431514 14 20.62198265524178 23.15844287544718 27.513538663426555 28.706035805884483 15 22.517695245586815 32.72892886316781 23.10531570507972 21.64806018616565 16 21.176509992908642 28.091286561101832 29.10586580297837 21.62633764301116 17 20.743485507423834 29.47695348344745 28.281519426433665 21.498041582695055 18 20.617862040750474 26.899158138570044 31.86601812796755 20.616961692711936 19 23.3878091793259 26.393830080159837 28.208455190042447 22.009905550471814 20 23.91016017052397 26.95785480902302 28.85709110276993 20.274893917683077 21 22.51521312783637 26.47365134514372 27.644102477674572 23.36703304934534 22 23.21270377215259 28.756775599630664 27.59982800816457 20.430692620052174 23 21.28106807409451 28.304726949539884 28.99017100449928 21.424033971866326 24 23.14589075676855 26.506957793730102 29.609029682889393 20.738121766611954 25 21.889483399750656 27.638369021990822 29.32216158675581 21.149985991502714 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 176.0 1 176.0 2 503.0 3 830.0 4 830.0 5 830.0 6 2131.5 7 3433.0 8 3433.0 9 3433.0 10 4324.0 11 5215.0 12 5215.0 13 5215.0 14 7475.5 15 9736.0 16 9736.0 17 9736.0 18 19014.5 19 28293.0 20 28293.0 21 28293.0 22 48757.5 23 69222.0 24 69222.0 25 69222.0 26 106874.0 27 144526.0 28 144526.0 29 144526.0 30 189088.5 31 233651.0 32 233651.0 33 233651.0 34 259525.5 35 285400.0 36 285400.0 37 285400.0 38 299916.5 39 314433.0 40 314433.0 41 314433.0 42 329019.5 43 343606.0 44 343606.0 45 343606.0 46 358537.5 47 373469.0 48 373469.0 49 373469.0 50 353166.0 51 332863.0 52 332863.0 53 332863.0 54 288482.0 55 244101.0 56 244101.0 57 244101.0 58 207721.0 59 171341.0 60 171341.0 61 171341.0 62 141877.0 63 112413.0 64 112413.0 65 112413.0 66 85326.0 67 58239.0 68 58239.0 69 58239.0 70 43268.0 71 28297.0 72 28297.0 73 28297.0 74 19829.0 75 11361.0 76 11361.0 77 11361.0 78 7873.5 79 4386.0 80 4386.0 81 4386.0 82 2912.5 83 1439.0 84 1439.0 85 1439.0 86 902.0 87 365.0 88 365.0 89 365.0 90 222.5 91 80.0 92 80.0 93 80.0 94 45.5 95 11.0 96 11.0 97 11.0 98 5.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0890205791667357 2 0.04375404679918441 3 0.020130462683739987 4 0.005833872906557921 5 0.018726011798827897 6 0.061687804252677274 7 0.07728081023131667 8 0.05376526079932702 9 0.022471214158593475 10 0.05146052088562512 11 0.005941907590012698 12 2.5208092806114476E-4 13 0.006770173496499316 14 8.642774676382106E-4 15 0.015629017539790974 16 0.009615086827475094 17 0.005545780417345185 18 0.006554104129589764 19 0.0021246821079439343 20 0.0011883815180025396 21 2.880924892127369E-4 22 3.6011561151592106E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2776886.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.97978721193264 #Duplication Level Percentage of deduplicated Percentage of total 1 72.41688480976968 36.193804933433256 2 14.864422911181666 14.858413882580718 3 5.427228188964509 8.13755130105146 4 2.5969500968575066 5.19180052963784 5 1.3781326062024697 3.4439387203912792 6 0.8470521508819584 2.540129175509408 7 0.5011506141240453 1.75331807385344 8 0.36872387995491496 1.4742992848083873 9 0.27823243109496393 1.2515397931426506 >10 1.1915347211135865 10.214295159926648 >50 0.06903231055129115 2.403783806605901 >100 0.05040776493107854 4.91540752523068 >500 0.005917578722015042 2.0159244160529255 >1k 0.003824776491005243 3.392431597979468 >5k 4.329935650194615E-4 1.7490196590246274 >10k+ 7.216559416991025E-5 0.46434214077132424 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 12874 0.46361283826559674 No Hit GTCCTACAGTGGACATTTCTAAATT 9398 0.3384366517026626 No Hit TATCAACGCAGAGTACTTTTTTTTT 8890 0.32014277863765384 No Hit CTGTAGGACGTGGAATATGGCAAGA 8281 0.2982117378963342 No Hit GTCCTAAAGTGTGTATTTCTCATTT 7660 0.27584855842119554 No Hit GGTATCAACGCAGAGTACTTTTTTT 7262 0.2615159570828619 No Hit CTTTAGGACGTGAAATATGGCGAGG 7001 0.25211693962229637 No Hit GTCCTACAGTGTGCATTTCTCATTT 4529 0.16309636045556067 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 4508 0.1623401176713772 No Hit CTGAAGGACCTGGAATATGGCGAGA 3626 0.13057792073567298 No Hit CTGTAGGACCTGGAATATGGCGAGA 3033 0.10922306497277885 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2947 0.10612607071374194 No Hit ATTTAGAAATGTCCACTGTAGGACG 2837 0.10216479898706682 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.601156115159211E-5 0.0 4 0.0 0.0 0.0 3.601156115159211E-5 0.0 5 0.0 0.0 0.0 3.601156115159211E-5 0.0 6 0.0 0.0 0.0 3.601156115159211E-5 0.0 7 0.0 0.0 0.0 3.601156115159211E-5 0.0 8 0.0 0.0 0.0 3.601156115159211E-5 0.0 9 0.0 0.0 0.0 3.601156115159211E-5 0.0 10 0.0 0.0 0.0 7.202312230318422E-5 0.0 11 0.0 0.0 0.0 7.202312230318422E-5 0.0 12 0.0 0.0 0.0 7.202312230318422E-5 0.0 13 0.0 0.0 0.0 7.202312230318422E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2145 0.0 13.126702 1 TGTCGAG 180 0.0 12.656282 18 TAGGACC 1540 0.0 12.535486 4 GGACCGT 100 1.4253055E-7 12.362359 6 CCAACGA 160 3.6379788E-12 11.864838 19 GGCGAGG 2005 0.0 11.787909 19 ATGTCGA 210 0.0 11.752263 17 TCCAACG 275 0.0 11.735825 18 CCGCCCA 65 8.038475E-4 11.6903 9 TATAGCG 65 8.0823223E-4 11.682722 16 GTATCAA 5245 0.0 11.407656 1 CGATACT 60 0.0058332486 11.094625 4 CGTTTTA 60 0.005913621 11.074246 17 CCGTACA 200 0.0 10.916043 17 TGTAGGA 4445 0.0 10.7633705 2 CCCGTAC 205 0.0 10.649799 16 CTGTAGG 4485 0.0 10.583501 1 GACGTGG 2150 0.0 10.522247 7 TATGTCG 235 0.0 10.502021 16 GCCGGTT 155 3.7071004E-9 10.4125 11 >>END_MODULE