FastQCFastQC Report
Thu 26 May 2016
SRR1512879_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512879_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622357
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79570.30342931950150187No Hit
GTATCAACGCAGAGTACTTTTTTTT66090.25202518192603063No Hit
GTCCTAAAGTGTGTATTTCTCATTT59480.22681885037010596No Hit
GTCCTACAGTGTGCATTTCTCATTT58310.22235721528380764No Hit
CTGTAGGACGTGGAATATGGCAAGA55490.21160353071683222No Hit
CTTTAGGACGTGAAATATGGCGAGG49110.1872742727248807No Hit
TATCAACGCAGAGTACTTTTTTTTT38790.14792036324573657No Hit
CTGTAGGACCTGGAATATGGCGAGA38640.1473483587474932No Hit
GGTATCAACGCAGAGTACTTTTTTT37740.14391633175803295No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC15700.013.3712554
CGGTTTC2000.012.82296413
GGTATCA20700.012.4206351
AGAACCG701.0924856E-412.2130465
CTTATAC2100.012.2128123
AGGACCT25100.011.6183415
TGTAGGA39050.011.3597062
GTCCTAG4800.011.3079531
GTATCAA46150.011.2247731
GGACCTG24100.011.15446956
GTCCTAA18550.011.0881791
GTCCTAC40900.011.0824061
TCCAACG3600.011.08199718
GTAGGAC38200.011.0654193
CCAACGA2500.011.01867119
CTGTAGG39150.010.969711
TAGTACG700.001493257210.8560414
ATAGGAC4200.010.8558333
CATTTCT49900.010.69790514
GCCGGTT2400.010.68580311
Produced by FastQC (version 0.11.5)