##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512879_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2622357
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.7929877587224	31.0	30.0	34.0	22.0	34.0
2	28.951358644151043	31.0	30.0	34.0	22.0	34.0
3	29.158558502904068	31.0	30.0	34.0	22.0	34.0
4	32.56237461184728	35.0	33.0	37.0	25.0	37.0
5	32.80387872436896	35.0	35.0	37.0	26.0	37.0
6	32.70208632920689	37.0	33.0	37.0	25.0	37.0
7	32.61815534650698	37.0	33.0	37.0	25.0	37.0
8	32.578139055818866	37.0	33.0	37.0	24.0	37.0
9	33.892480314465196	39.0	34.0	39.0	23.0	39.0
10	33.739412673407934	38.0	34.0	39.0	22.0	39.0
11	33.84043362517003	39.0	34.0	39.0	23.0	39.0
12	33.72406236069307	38.0	34.0	39.0	22.0	39.0
13	33.719074100132055	39.0	34.0	39.0	21.0	39.0
14	34.58231163796539	39.0	34.0	41.0	18.0	41.0
15	34.7647143390469	39.0	34.0	41.0	20.0	41.0
16	34.72553813229854	39.0	34.0	41.0	19.0	41.0
17	34.49055944709283	39.0	34.0	41.0	17.0	41.0
18	34.494479203251124	39.0	34.0	41.0	17.0	41.0
19	34.6053066001311	39.0	34.0	41.0	18.0	41.0
20	34.57077545124481	39.0	34.0	41.0	18.0	41.0
21	34.46310361251348	39.0	34.0	41.0	18.0	41.0
22	34.490520932123275	39.0	34.0	41.0	18.0	41.0
23	34.1578107023567	39.0	34.0	41.0	10.0	41.0
24	34.23284739644526	39.0	34.0	41.0	9.0	41.0
25	33.95263802754545	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.38329233053629963
1101	2	0.08122780247578731
1101	3	0.49564677632814735
1101	4	0.496589023560972
1101	5	0.5644202695525351
1101	6	0.37546099347471085
1101	7	0.16815902446638376
1101	8	0.48244483180960884
1101	9	0.5136895666848176
1101	10	0.553181568243204
1101	11	0.5472677210203116
1101	12	0.38982888897724166
1101	13	0.768349383935508
1101	14	0.8524178057941683
1101	15	0.6420536560393586
1101	16	0.585862019791378
1101	17	0.0551263194572158
1101	18	0.534892329090745
1101	19	0.0802151930289341
1101	20	-0.6520500698497358
1101	21	0.48925808541400784
1101	22	0.5518436386439234
1101	23	0.09957339895846218
1101	24	0.5972031577678862
1101	25	0.4020230943664984
1102	1	0.9505464015463687
1102	2	0.9534933607986851
1102	3	0.9865723672694422
1102	4	1.1113220940102693
1102	5	0.9949806605188272
1102	6	0.9027641570912124
1102	7	0.8812305531081108
1102	8	0.8840556525255607
1102	9	1.2213591638132755
1102	10	1.222741095463114
1102	11	1.088440803837436
1102	12	1.1614600819887073
1102	13	1.0960489565528206
1102	14	1.3130113724120562
1102	15	1.3435134361073011
1102	16	1.290468062986271
1102	17	1.4293858253336964
1102	18	1.4584507071736041
1102	19	1.2658159079393414
1102	20	1.4247254321064773
1102	21	1.5133103651985778
1102	22	1.3640734135748218
1102	23	1.413317705684996
1102	24	0.7075084121754145
1102	25	1.1737182635815273
1103	1	0.5441969739668338
1103	2	0.6841974887874578
1103	3	0.8445737282263757
1103	4	0.9664423203315025
1103	5	0.7355141821842395
1103	6	0.43736002588809697
1103	7	0.32511905874798686
1103	8	0.6536279973809584
1103	9	0.7080228790070748
1103	10	0.9433893289584532
1103	11	0.6720899434661831
1103	12	0.8234392683790404
1103	13	0.9260904476504521
1103	14	1.0004636696222562
1103	15	0.916852201295896
1103	16	0.9217131511849459
1103	17	0.6260835982860513
1103	18	0.9770647668295354
1103	19	0.8796033204281031
1103	20	0.994667726127048
1103	21	0.854898908278436
1103	22	0.8723884205780692
1103	23	0.23183407263011446
1103	24	0.4686389329945513
1103	25	0.7831996229091658
1104	1	0.8632583544794734
1104	2	0.8381256051494042
1104	3	0.8620821899635587
1104	4	0.9908965062251269
1104	5	0.7928249292003429
1104	6	0.8171122194343425
1104	7	1.008296606042073
1104	8	0.7561809367986143
1104	9	1.0961720683004046
1104	10	1.1579361357668745
1104	11	0.7663948237195441
1104	12	1.1965798911603898
1104	13	1.1443086256700141
1104	14	1.379878144306474
1104	15	1.0011675089866188
1104	16	1.0400564959840821
1104	17	1.322132681678653
1104	18	1.1056396983193295
1104	19	0.7988590503581037
1104	20	0.812723143546684
1104	21	0.9403100038974728
1104	22	0.8899248431100872
1104	23	1.1908129594151688
1104	24	1.1652843882394563
1104	25	0.7621608449773021
1105	1	0.42186244415991325
1105	2	0.6789331385868245
1105	3	0.6738544001917894
1105	4	0.8565284411335483
1105	5	0.634183643280231
1105	6	0.8531231594325064
1105	7	0.8129713433705277
1105	8	0.7242936149678343
1105	9	0.4990790312827684
1105	10	0.7621978411314672
1105	11	0.7385097557964428
1105	12	0.9541622831368244
1105	13	0.8589165726927277
1105	14	0.9864377165435698
1105	15	0.9496244030022325
1105	16	0.860011755978114
1105	17	1.2463229948556176
1105	18	1.0618671019879855
1105	19	0.7901275251309059
1105	20	0.9907661110442447
1105	21	0.8028221272586507
1105	22	0.8414684716862695
1105	23	0.9858062225920889
1105	24	1.0215985114057347
1105	25	1.315147195801778
1106	1	0.6620848105831207
1106	2	0.6673847165179687
1106	3	0.6871900303990834
1106	4	0.8120639761882131
1106	5	0.7225823280571717
1106	6	0.7720445719399365
1106	7	0.6347733310672439
1106	8	0.6541191738957224
1106	9	0.8546276770341166
1106	10	0.6373074461804862
1106	11	0.5353733343686073
1106	12	0.6488749568394852
1106	13	0.7471414406568826
1106	14	1.041935183039051
1106	15	0.7466826403916684
1106	16	0.9119384967077053
1106	17	1.0016189094099062
1106	18	0.9215835856741137
1106	19	0.9105851159678267
1106	20	0.9274919872685103
1106	21	0.868654650868848
1106	22	0.8112179160141579
1106	23	1.0258880493525027
1106	24	0.7689375591513112
1106	25	0.8817442181473893
1107	1	0.8670503372417215
1107	2	0.8856900832748877
1107	3	0.904635887031823
1107	4	1.0709958656903211
1107	5	0.8588349708230183
1107	6	0.947528293911553
1107	7	0.6768892056664768
1107	8	0.8155873532516154
1107	9	0.786265764837303
1107	10	0.9525424726940983
1107	11	0.8829297683455337
1107	12	0.9172197274280265
1107	13	0.7306843426036025
1107	14	1.0187103690850279
1107	15	0.787083564507796
1107	16	0.9270837332661515
1107	17	1.0848589114848792
1107	18	1.164414147497311
1107	19	0.9698314829126886
1107	20	1.0981857170237035
1107	21	0.8264086045243033
1107	22	0.9488266410077273
1107	23	1.1250655558692841
1107	24	0.9081741295030383
1107	25	1.1937312075330766
1108	1	0.7743741222145957
1108	2	0.8253196315132278
1108	3	0.813269138655194
1108	4	0.7781025407342739
1108	5	0.6159674786663274
1108	6	0.6330480983161451
1108	7	0.6709088591654577
1108	8	0.6669547696232385
1108	9	0.806905596558039
1108	10	0.7987146678186718
1108	11	0.5519687610506949
1108	12	0.6679663534032443
1108	13	0.6364925417104885
1108	14	0.7131967349663384
1108	15	0.6928930348854223
1108	16	0.45057979252909774
1108	17	0.8430552995851173
1108	18	0.9069075682378269
1108	19	0.6968182898382693
1108	20	0.8382315948734202
1108	21	0.8026646763589653
1108	22	0.8273140761433098
1108	23	1.0710725493607853
1108	24	0.9282072183690815
1108	25	1.0465342352839357
1109	1	0.7576752783989669
1109	2	0.8141916708452897
1109	3	0.8599826664316019
1109	4	0.9540800899433819
1109	5	0.8045092851173408
1109	6	0.9720187484612097
1109	7	0.8627256104252083
1109	8	0.7965982469642228
1109	9	0.9766753857319017
1109	10	1.0436146815561216
1109	11	0.9688273524517328
1109	12	0.759977906043197
1109	13	0.8587610983202083
1109	14	0.9374464843345436
1109	15	0.8334767618117027
1109	16	0.5861356576539123
1109	17	0.9840295610096135
1109	18	0.9944750723587816
1109	19	0.872686473480158
1109	20	0.7748113262352305
1109	21	0.8667636364600142
1109	22	0.9175934486383355
1109	23	1.1930312917828871
1109	24	0.9153425893069738
1109	25	0.47832307304213373
1110	1	0.6428845055165553
1110	2	0.6402466222217527
1110	3	0.47873156097077896
1110	4	0.5979583896567604
1110	5	0.49008258198232113
1110	6	0.6464631724470706
1110	7	0.519768225835314
1110	8	0.5821489463966074
1110	9	0.8655022665217942
1110	10	0.6603168066633174
1110	11	0.5807051209859537
1110	12	0.668366073023158
1110	13	0.7553349369656104
1110	14	0.772767220017677
1110	15	0.6179964045602446
1110	16	0.7224871710402425
1110	17	0.8815942831253594
1110	18	0.17395186088977255
1110	19	0.6027061304567098
1110	20	0.6820310106634793
1110	21	0.7219862379099382
1110	22	0.818122898114666
1110	23	0.9013107679018617
1110	24	0.8044794175982872
1110	25	0.9750892409471916
1111	1	0.39057493872432403
1111	2	0.4694755029602824
1111	3	0.5114506376903627
1111	4	0.3949264236465595
1111	5	0.3821331693791734
1111	6	0.23281477289967256
1111	7	0.23278333103534976
1111	8	0.43443368136238547
1111	9	0.4955861898179421
1111	10	0.4624412521893291
1111	11	0.1604392052337147
1111	12	-0.041217108215242604
1111	13	0.3699583995651565
1111	14	0.39172267751511214
1111	15	0.36121909555216547
1111	16	0.42995583948320615
1111	17	-0.0382684465379981
1111	18	0.34098032726789285
1111	19	0.26840965334793054
1111	20	0.35665958861927294
1111	21	0.3046804414166857
1111	22	0.08415453220611369
1111	23	0.3138760031792813
1111	24	0.38139343408827386
1111	25	0.33731970181761284
1112	1	0.07588823658348787
1112	2	0.024813522759000506
1112	3	0.039438450305503636
1112	4	0.14158098628404048
1112	5	0.09092129027937546
1112	6	-0.1649246736339336
1112	7	-0.32919532096595816
1112	8	-0.15408404202835868
1112	9	-0.27216477703694153
1112	10	-0.028064329422150536
1112	11	-0.20240934612870376
1112	12	-0.0719335644033805
1112	13	-0.12702464879324538
1112	14	-0.21576598955022774
1112	15	-0.3055687859732359
1112	16	-0.04978224041014556
1112	17	0.16030871359316023
1112	18	-0.13755662357069554
1112	19	-0.09954770607965457
1112	20	-0.20206617372781466
1112	21	-0.16324551779816687
1112	22	-0.1103745862541885
1112	23	-0.02920459771910089
1112	24	-0.04330769927610589
1112	25	-0.011930146227392413
1113	1	-0.09356500981982308
1113	2	-0.006601317598256884
1113	3	-0.01713377709370434
1113	4	0.11047925694695948
1113	5	0.04611163631906123
1113	6	-0.29909307080779257
1113	7	-0.26862713605753896
1113	8	-0.11716299811838837
1113	9	-0.26409447592056523
1113	10	-0.21271044842188758
1113	11	-0.1850564950361786
1113	12	-0.47282347142674297
1113	13	-0.22544849775542275
1113	14	-0.10575757455612944
1113	15	-0.3145251696081246
1113	16	-0.35417869833360527
1113	17	-0.11241862315493023
1113	18	-0.40482223153207997
1113	19	-0.7461543572727862
1113	20	-0.41235695202321665
1113	21	-0.19667542800464588
1113	22	-0.7330982339260714
1113	23	-4.141072989681664
1113	24	-1.8544753726109562
1113	25	-1.15544945775585
1114	1	-0.7513107309088767
1114	2	-0.9358093461829284
1114	3	-0.8318493087547161
1114	4	-0.7879015465951404
1114	5	-0.7553123145731249
1114	6	-1.4318820293619723
1114	7	-1.2557233718233576
1114	8	-1.155887663804883
1114	9	-1.40397827412324
1114	10	-1.0057859950162538
1114	11	-1.1627237783028406
1114	12	-2.0428580658380078
1114	13	-1.2563824292218229
1114	14	-3.016742609687679
1114	15	-1.8105613283810698
1114	16	-1.8056392057479016
1114	17	-1.7338996972197265
1114	18	-1.7250358907533823
1114	19	-1.8098662492625834
1114	20	-1.3386078864674076
1114	21	-1.1849559774489506
1114	22	-1.3614206713294053
1114	23	-5.4801079307917036
1114	24	-2.6786415531890775
1114	25	-2.7529162594619088
1115	1	-1.625486225090114
1115	2	-1.5596684969136447
1115	3	-1.210256624203808
1115	4	-1.3285342443764385
1115	5	-1.2174910206060083
1115	6	-1.2396742512843808
1115	7	-1.760993767951728
1115	8	-2.102186907844679
1115	9	-3.281324438180093
1115	10	-3.961711926583792
1115	11	-2.632028888976805
1115	12	-2.8706446082666552
1115	13	-2.19312706743154
1115	14	-2.974202385380316
1115	15	-2.5205205709411516
1115	16	-2.717405223661153
1115	17	-2.6127534839841147
1115	18	-5.184749837936266
1115	19	-3.170076390531829
1115	20	-3.339153748923561
1115	21	-3.027148903976755
1115	22	-2.593161153281237
1115	23	-3.1402158520534655
1115	24	-2.860871864277364
1115	25	-3.152821655633801
1116	1	-1.6395298376597722
1116	2	-1.5875136820680211
1116	3	-2.0720400716072405
1116	4	-1.9106759498481871
1116	5	-2.0330084789755105
1116	6	-4.190700342017237
1116	7	-2.673276284411365
1116	8	-3.1216869259396134
1116	9	-4.249520729853128
1116	10	-3.432699861314557
1116	11	-3.0124611364161034
1116	12	-2.9562703175475207
1116	13	-4.155213321386093
1116	14	-7.247300081274172
1116	15	-5.120573451357412
1116	16	-3.986578695733108
1116	17	-4.908912172765849
1116	18	-3.72129944208935
1116	19	-3.442566960911126
1116	20	-4.165264467227587
1116	21	-4.798087145160068
1116	22	-4.371842837481985
1116	23	-3.5023737295340247
1116	24	-4.552445188997151
1116	25	-4.237925150548584
1201	1	0.6192164961064854
1201	2	0.7199767570009783
1201	3	1.1494240931828799
1201	4	1.0568523551084397
1201	5	0.6242427793866909
1201	6	1.0966750651528656
1201	7	1.071692515814206
1201	8	1.2050625014583574
1201	9	1.5330393197848053
1201	10	1.425899244781128
1201	11	1.477311578297126
1201	12	1.3169884029545713
1201	13	1.4044169695761894
1201	14	1.6163493091883652
1201	15	1.5675795533542356
1201	16	1.566822564184129
1201	17	1.7753673665205199
1201	18	1.5876581330544681
1201	19	1.6863607060417891
1201	20	1.6389731090882975
1201	21	1.8317534978506131
1201	22	1.544540687515493
1201	23	1.924442063409586
1201	24	1.7611185199989308
1201	25	1.7031337885845446
1202	1	0.8023869790147877
1202	2	0.823933783588064
1202	3	0.878103999133323
1202	4	1.0616474355931516
1202	5	0.8841141485312036
1202	6	1.1565541029535495
1202	7	1.1683967299578129
1202	8	1.114096178434302
1202	9	1.32508012282333
1202	10	1.236669221268734
1202	11	1.2602386898905422
1202	12	1.47519104357675
1202	13	1.3409500135262675
1202	14	1.8532025557067229
1202	15	1.4753325647486975
1202	16	1.1994765703335233
1202	17	1.538944577589433
1202	18	1.505606984564686
1202	19	1.5120535980656626
1202	20	1.5035226478837345
1202	21	1.5302510200065171
1202	22	1.492633145464488
1202	23	1.5783519928724203
1202	24	1.558193434088274
1202	25	1.946432468273457
1203	1	1.0209856649543418
1203	2	1.1983022461373416
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2211	18	-0.31725879844955074
2211	19	-0.21036564592059648
2211	20	-0.272571215641058
2211	21	-0.09268388426446705
2211	22	-0.0789768692630517
2211	23	-0.058763372861506014
2211	24	-0.4048117696426985
2211	25	0.13081381338882636
2212	1	-0.06261798281727238
2212	2	-0.011429575190561536
2212	3	-0.3409141942508995
2212	4	-0.0715370427783455
2212	5	-0.34667681838991626
2212	6	-0.3724831744001378
2212	7	-0.24236459319740078
2212	8	-0.20550598441557355
2212	9	-0.17306224358888755
2212	10	-0.36957416295518186
2212	11	-0.5529909538753586
2212	12	-0.17429890553266603
2212	13	-0.11993204754243436
2212	14	-0.10977696083271837
2212	15	-0.36733613754137906
2212	16	-0.4559254652898872
2212	17	-0.09434817050217248
2212	18	0.01470647565883354
2212	19	-0.19321548590380644
2212	20	-0.13703345898649388
2212	21	0.002544022551049352
2212	22	-0.01528517514619665
2212	23	-0.006586938425293454
2212	24	0.006234401009393764
2212	25	0.05075740466022438
2213	1	-0.28718176657240946
2213	2	-0.12334567040111111
2213	3	-0.3686961577663368
2213	4	-0.15746419851683413
2213	5	-0.16543777741215848
2213	6	-0.06393398051707067
2213	7	-0.25704743729722423
2213	8	-0.08548251459151146
2213	9	-0.5129856808951914
2213	10	-0.16712222769965024
2213	11	-0.352420916291301
2213	12	-0.3021450511906494
2213	13	-0.3371641780482193
2213	14	-0.4540186571276692
2213	15	-0.30426696455231195
2213	16	-0.3022173184246171
2213	17	-0.26353374672216745
2213	18	-0.24775346103035645
2213	19	-0.3412850698562053
2213	20	-0.09654475621134395
2213	21	-0.2754044597142027
2213	22	-0.5695883654792624
2213	23	-0.2774277200011994
2213	24	0.009148984413840822
2213	25	0.16102953424977073
2214	1	-2.7278416060941417
2214	2	-1.5240909231392514
2214	3	-1.7656432516846472
2214	4	-3.4780675113384554
2214	5	-1.9666561970118508
2214	6	-1.6546817760664787
2214	7	-1.3598760655094289
2214	8	-1.2768351758071788
2214	9	-1.2196497790201022
2214	10	-1.2105465476244106
2214	11	-1.2322005408172956
2214	12	-1.2033289149261606
2214	13	-1.48156030488407
2214	14	-1.406925319163598
2214	15	-1.759104399579762
2214	16	-1.5881474022145525
2214	17	-3.7047037824776012
2214	18	-3.4858421256420016
2214	19	-2.230830768704749
2214	20	-1.9608075412472203
2214	21	-1.9736033571381597
2214	22	-2.8544776038771467
2214	23	-1.8200640952290925
2214	24	-2.890971101862565
2214	25	-2.7574999872368764
2215	1	-1.762692578996262
2215	2	-3.4720842642940717
2215	3	-2.118950144513086
2215	4	-3.672429912473145
2215	5	-2.3677267354466984
2215	6	-2.0912366889433187
2215	7	-2.2472831963773636
2215	8	-1.737132919171593
2215	9	-3.968540871651811
2215	10	-2.512626174679699
2215	11	-2.156138842337814
2215	12	-2.1136594168284084
2215	13	-2.1012975002858845
2215	14	-1.8506044424516546
2215	15	-2.113587877240249
2215	16	-2.1220667077511877
2215	17	-2.608924299021986
2215	18	-2.082940713409535
2215	19	-2.158392074125871
2215	20	-2.0348011090223395
2215	21	-2.6264480591831685
2215	22	-2.461177904259273
2215	23	-2.5626351157831984
2215	24	-2.527216142338979
2215	25	-3.477375634857296
2216	1	-3.1268722267613853
2216	2	-3.302453220022045
2216	3	-4.656913209049588
2216	4	-5.0635848147076885
2216	5	-4.907267560253395
2216	6	-3.7743566070344166
2216	7	-5.534213258008492
2216	8	-3.5191177806511362
2216	9	-4.299068974649597
2216	10	-3.2044418497300597
2216	11	-3.4428972908555444
2216	12	-3.851164070743348
2216	13	-3.4648463763654256
2216	14	-4.432651716254771
2216	15	-3.872377543554549
2216	16	-4.2434578460323
2216	17	-3.855565169702988
2216	18	-3.4939433162776723
2216	19	-4.068237015652752
2216	20	-4.6973968467011
2216	21	-4.441074732099377
2216	22	-4.065170464643817
2216	23	-3.9668068518929225
2216	24	-4.380715470845537
2216	25	-4.47486127420548
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	131101.0
3	5.0
4	1172.0
5	7188.0
6	8259.0
7	7450.0
8	6470.0
9	5968.0
10	5413.0
11	5152.0
12	4825.0
13	4669.0
14	4785.0
15	4780.0
16	5101.0
17	5542.0
18	6183.0
19	7532.0
20	9531.0
21	12320.0
22	15393.0
23	18522.0
24	21747.0
25	24731.0
26	27587.0
27	30400.0
28	34712.0
29	40713.0
30	49215.0
31	61021.0
32	77203.0
33	98161.0
34	127973.0
35	168292.0
36	231601.0
37	361557.0
38	990083.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.50492706308929	18.687713393507288	12.49552789579031	24.31183164761311
2	14.396760133069778	19.817526752585636	43.35114487222563	22.434568242118957
3	19.64085288858057	25.572775419406042	28.461069958784957	26.32530173322843
4	11.932588957626114	16.24053274082278	38.619628895263006	33.207249406288106
5	12.680566340252659	39.27860419492088	35.17445275183941	12.866376712987046
6	31.39492947899139	36.82835022018614	18.71435261000434	13.06236769081813
7	28.77796957069469	31.531200780978384	21.646389749582397	18.044439898744535
8	25.253783805552725	37.69704437728537	20.396915466983963	16.652256350177943
9	27.923938108116154	14.874997233016638	20.010671014456246	37.19039364441096
10	15.724859331953688	29.10475504179774	33.95792668544022	21.21245894080835
11	34.62421281630472	22.248005801305293	24.879088584405178	18.24869279798481
12	24.275573500733444	24.62551973772354	31.739909940755723	19.358996820787294
13	29.603475169157644	22.293391812575354	25.572239254496527	22.530893763770475
14	20.892612603260048	22.880678634730796	27.630908016689542	28.595800745319615
15	22.88537884298757	32.18537632405935	23.115097463439284	21.81414736951379
16	21.489631788419175	28.196451770984886	28.96954495283429	21.34437148776165
17	21.117340996996344	29.186058983689268	28.124836581598124	21.57176343771626
18	20.92075172660989	27.38859630137316	31.07676404696822	20.613887925048736
19	23.47478713987831	26.707566243611907	27.82004593282296	21.997600683686823
20	24.03265107956506	27.175809864638328	28.58771027166993	20.203828784126685
21	23.21969348574194	26.657016140143046	27.03908081597199	23.084209558143023
22	23.419052937635485	28.748354323305758	27.238473392237328	20.594119346821422
23	22.062357534035336	28.21253593615693	28.208031988714175	21.51707454109356
24	23.191789992603116	26.75378298350167	28.990214585984393	21.064212437910825
25	22.322356516339273	27.72228025787422	28.675243899227375	21.28011932655913
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	338.0
1	338.0
2	740.5
3	1143.0
4	1143.0
5	1143.0
6	1902.5
7	2662.0
8	2662.0
9	2662.0
10	3233.5
11	3805.0
12	3805.0
13	3805.0
14	6091.0
15	8377.0
16	8377.0
17	8377.0
18	16611.0
19	24845.0
20	24845.0
21	24845.0
22	42163.0
23	59481.0
24	59481.0
25	59481.0
26	91955.0
27	124429.0
28	124429.0
29	124429.0
30	166538.0
31	208647.0
32	208647.0
33	208647.0
34	236870.5
35	265094.0
36	265094.0
37	265094.0
38	285425.0
39	305756.0
40	305756.0
41	305756.0
42	320247.0
43	334738.0
44	334738.0
45	334738.0
46	347003.0
47	359268.0
48	359268.0
49	359268.0
50	341518.5
51	323769.0
52	323769.0
53	323769.0
54	280844.0
55	237919.0
56	237919.0
57	237919.0
58	201073.0
59	164227.0
60	164227.0
61	164227.0
62	133725.0
63	103223.0
64	103223.0
65	103223.0
66	78378.0
67	53533.0
68	53533.0
69	53533.0
70	39334.0
71	25135.0
72	25135.0
73	25135.0
74	17795.0
75	10455.0
76	10455.0
77	10455.0
78	7176.0
79	3897.0
80	3897.0
81	3897.0
82	2561.0
83	1225.0
84	1225.0
85	1225.0
86	763.0
87	301.0
88	301.0
89	301.0
90	181.5
91	62.0
92	62.0
93	62.0
94	41.5
95	21.0
96	21.0
97	21.0
98	14.0
99	7.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34068587915375365
2	0.010448615501245635
3	0.009762210103353586
4	0.011211288165570134
5	0.012545965328138007
6	0.07908915529045053
7	0.07832648262612603
8	0.10318961148310471
9	0.08294065224528925
10	0.08995724075707465
11	0.0860676101690197
12	0.09632555750418421
13	0.08267371681277569
14	0.08667774830047931
15	0.08370332490961375
16	0.0851524029718303
17	0.09716449743494115
18	0.10021518809223916
19	0.09319859958045376
20	0.0920164569507508
21	0.08084330241839688
22	0.099681317227212
23	0.0927791296150753
24	0.08904203355988524
25	0.08847002906164188
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2622357.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.27630959886505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37163966847949	41.66266089288971
2	13.877103164189409	15.34150101678247
3	4.778094811680314	7.923463443095156
4	2.133901064758664	4.718167036357907
5	1.1610573196540004	3.208948193161148
6	0.6783733514068528	2.2498785237590924
7	0.4220170800286462	1.6329282740170716
8	0.30200931287820176	1.3355168224316787
9	0.212916531864772	1.0592316120666376
>10	0.9590286488270698	9.173201044703841
>50	0.05711413947198842	2.1587259923914996
>100	0.039453028109273994	4.190364225562033
>500	0.0045140201174786055	1.7285097421761475
>1k	0.0024306262170783574	2.3925741027755487
>5k	3.472323167254796E-4	1.2243290778302074
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	7957	0.30342931950150187	No Hit
GTATCAACGCAGAGTACTTTTTTTT	6609	0.25202518192603063	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5948	0.22681885037010596	No Hit
GTCCTACAGTGTGCATTTCTCATTT	5831	0.22235721528380764	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5549	0.21160353071683222	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4911	0.1872742727248807	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3879	0.14792036324573657	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3864	0.1473483587474932	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3774	0.14391633175803295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	3.813363321622495E-5	0.0	0.0	0.0	0.0
4	3.813363321622495E-5	0.0	0.0	3.813363321622495E-5	0.0
5	3.813363321622495E-5	0.0	0.0	3.813363321622495E-5	0.0
6	7.62672664324499E-5	0.0	0.0	3.813363321622495E-5	0.0
7	7.62672664324499E-5	0.0	0.0	3.813363321622495E-5	0.0
8	7.62672664324499E-5	0.0	0.0	3.813363321622495E-5	0.0
9	7.62672664324499E-5	0.0	0.0	3.813363321622495E-5	0.0
10	7.62672664324499E-5	0.0	0.0	3.813363321622495E-5	0.0
11	7.62672664324499E-5	0.0	0.0	3.813363321622495E-5	0.0
12	7.62672664324499E-5	0.0	0.0	3.813363321622495E-5	0.0
13	7.62672664324499E-5	0.0	0.0	3.813363321622495E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1570	0.0	13.371255	4
CGGTTTC	200	0.0	12.822964	13
GGTATCA	2070	0.0	12.420635	1
AGAACCG	70	1.0924856E-4	12.213046	5
CTTATAC	210	0.0	12.212812	3
AGGACCT	2510	0.0	11.618341	5
TGTAGGA	3905	0.0	11.359706	2
GTCCTAG	480	0.0	11.307953	1
GTATCAA	4615	0.0	11.224773	1
GGACCTG	2410	0.0	11.1544695	6
GTCCTAA	1855	0.0	11.088179	1
GTCCTAC	4090	0.0	11.082406	1
TCCAACG	360	0.0	11.081997	18
GTAGGAC	3820	0.0	11.065419	3
CCAACGA	250	0.0	11.018671	19
CTGTAGG	3915	0.0	10.96971	1
TAGTACG	70	0.0014932572	10.856041	4
ATAGGAC	420	0.0	10.855833	3
CATTTCT	4990	0.0	10.697905	14
GCCGGTT	240	0.0	10.685803	11
>>END_MODULE