FastQCFastQC Report
Thu 26 May 2016
SRR1512879_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512879_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2622357
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75800.28905293977898505No Hit
GTCCTACAGTGGACATTTCTAAATT72430.2762019053851173No Hit
CTGTAGGACGTGGAATATGGCAAGA58040.22132760718696956No Hit
GTCCTACAGTGTGCATTTCTCATTT55650.21221366884829185No Hit
GTCCTAAAGTGTGTATTTCTCATTT53900.20554028303545246No Hit
CTTTAGGACGTGAAATATGGCGAGG53220.20294719597674918No Hit
TATCAACGCAGAGTACTTTTTTTTT50970.19436712850309854No Hit
GGTATCAACGCAGAGTACTTTTTTT42120.16061886310673948No Hit
CTGTAGGACCTGGAATATGGCGAGA39240.14963637674046668No Hit
CTGAAGGACCTGGAATATGGCGAGA27850.10620216850718647No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATACG456.714356E-414.7922934
CGTCTAG400.005236800814.2678121
TGCGCAT655.4675722E-513.1506910
TAGGACC15400.012.905524
TCCAACG3050.012.76036718
GGTATCA19100.012.4002981
CCAACGT1353.765308E-1011.95302319
GGCGAGG14400.011.86513219
TGTAGGA38150.011.7420172
AGGACCT25450.011.5083465
GGACCTG23800.011.3470536
GTAGGAC38000.011.2377423
CTGTAGG37850.011.2333111
CAACGAA855.3690557E-511.1676117
TGGCGAG34700.011.13381718
GTATCAA41900.011.0101651
GTCGAGG1900.010.9908619
TACACGG2250.010.9885615
GTCCTAC39800.010.9697261
CACCTTT8900.010.77276214
Produced by FastQC (version 0.11.5)