##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512879_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2622357 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.242230558234443 31.0 30.0 34.0 26.0 34.0 2 30.274906887201094 31.0 30.0 34.0 26.0 34.0 3 30.456906134443173 31.0 31.0 34.0 26.0 34.0 4 34.02815825610319 37.0 35.0 37.0 30.0 37.0 5 33.604787220046695 37.0 35.0 37.0 28.0 37.0 6 33.46407220679717 37.0 35.0 37.0 28.0 37.0 7 33.3051663064945 37.0 35.0 37.0 27.0 37.0 8 33.25020277559463 37.0 35.0 37.0 27.0 37.0 9 34.588525894834305 39.0 35.0 39.0 27.0 39.0 10 34.51339996804401 39.0 35.0 39.0 27.0 39.0 11 34.48963279980567 39.0 35.0 39.0 27.0 39.0 12 34.35082141752629 39.0 35.0 39.0 26.0 39.0 13 34.3428953418623 39.0 35.0 39.0 26.0 39.0 14 35.42934314435449 40.0 36.0 41.0 26.0 41.0 15 35.43975362622252 40.0 36.0 41.0 26.0 41.0 16 35.43838424745372 40.0 36.0 41.0 26.0 41.0 17 35.42997311197522 40.0 36.0 41.0 26.0 41.0 18 35.353631484958 40.0 36.0 41.0 25.0 41.0 19 35.369927511776616 40.0 36.0 41.0 25.0 41.0 20 35.33784034744316 40.0 36.0 41.0 25.0 41.0 21 35.3008446218421 40.0 36.0 41.0 25.0 41.0 22 35.26927226155706 40.0 36.0 41.0 25.0 41.0 23 35.11177311098375 40.0 36.0 41.0 24.0 41.0 24 35.0728531622506 40.0 36.0 41.0 24.0 41.0 25 35.01547043365949 40.0 36.0 41.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4844745056749282 1101 2 0.28694553625707186 1101 3 0.3100840773953095 1101 4 0.22467032479066518 1101 5 0.272419832657242 1101 6 0.36132699466860174 1101 7 0.44591011546275183 1101 8 0.19851731113115534 1101 9 0.48249207202529476 1101 10 0.21261123616086763 1101 11 0.49649620119020454 1101 12 0.6353503984172306 1101 13 0.5671795129752368 1101 14 0.7254039249840574 1101 15 0.3856571329623577 1101 16 0.6306745744246882 1101 17 0.7011773273537045 1101 18 0.5536240520188684 1101 19 0.33980532286351206 1101 20 0.4328314671917255 1101 21 0.4742809536091812 1101 22 0.44900124540701114 1101 23 0.6757370729799774 1101 24 0.5730565638543013 1101 25 0.7154137389265003 1102 1 0.9497130422348263 1102 2 0.9763520237416792 1102 3 0.698058701700802 1102 4 0.7421800571537247 1102 5 0.9828606949753151 1102 6 0.954258516866723 1102 7 0.8706869494224563 1102 8 0.9597078717601732 1102 9 1.12443802254802 1102 10 1.0414583227548917 1102 11 1.0905262069764703 1102 12 1.0722452450731694 1102 13 1.2572030394463738 1102 14 1.0610372231237122 1102 15 1.1829009217927293 1102 16 1.1496103809996043 1102 17 1.2327292953726356 1102 18 0.5804138505600989 1102 19 0.9697996934986222 1102 20 1.2193392916301988 1102 21 1.1613601545261147 1102 22 0.9296401931999938 1102 23 1.1373504716799445 1102 24 1.0911008288948594 1102 25 1.1849532887991288 1103 1 0.725529613141294 1103 2 0.8343743365121732 1103 3 0.6720226247600145 1103 4 0.44757914430532963 1103 5 0.6777669577326577 1103 6 0.7222829651605736 1103 7 0.7085786035603334 1103 8 0.8089045258726699 1103 9 0.8419903327847749 1103 10 0.8200825017463629 1103 11 0.9195312990479749 1103 12 1.1045287363844736 1103 13 1.0461860032795087 1103 14 1.135166951808138 1103 15 1.116673800750199 1103 16 1.0686404707969928 1103 17 1.1095287275654755 1103 18 1.075464537681981 1103 19 1.0692997910351707 1103 20 0.9559412250010908 1103 21 0.9917643332286872 1103 22 1.1286360287342418 1103 23 1.0512362405705105 1103 24 1.1189630320704538 1103 25 1.0617923472480513 1104 1 0.7410105732161547 1104 2 0.844208779832762 1104 3 0.725973572643472 1104 4 0.5869582019827604 1104 5 0.7423838541845171 1104 6 0.5860836816324877 1104 7 0.5394986137938602 1104 8 0.5526750546944967 1104 9 0.6881262072351788 1104 10 0.7080885718034793 1104 11 0.6861244669810134 1104 12 0.7090542567085976 1104 13 0.643674661017485 1104 14 0.8676943637081749 1104 15 0.9124596648482282 1104 16 0.836261119750155 1104 17 0.7531460662607756 1104 18 0.5129080453042718 1104 19 0.9106641902502659 1104 20 0.7947861501793767 1104 21 0.6900281993121169 1104 22 0.7029244202504259 1104 23 0.5895599189850884 1104 24 0.7798001544980764 1104 25 0.694117590716516 1105 1 0.588961752075658 1105 2 0.5625428678501336 1105 3 0.5748671996979873 1105 4 0.35912840582737715 1105 5 0.4678945416478726 1105 6 0.5053004579358387 1105 7 0.5639481017614898 1105 8 0.5158125359926231 1105 9 0.8427512829725643 1105 10 0.5694332865713392 1105 11 0.7565445723785515 1105 12 0.7495978505477936 1105 13 0.8178623766058166 1105 14 0.9471807604899993 1105 15 0.9329358566418762 1105 16 0.8827119068195088 1105 17 0.6323152190810646 1105 18 0.5763322450548429 1105 19 0.8524511529375189 1105 20 0.8145677252526085 1105 21 0.7463754609464672 1105 22 0.6974758166925596 1105 23 0.9797912642255255 1105 24 0.8762038929353508 1105 25 0.7385231379362196 1106 1 0.045996260104896436 1106 2 0.4393755036768887 1106 3 -1.4277439932090275 1106 4 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-2.6837272937681576 2216 23 -3.8522244564482797 2216 24 -3.2183145480817856 2216 25 -3.2078740639835566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76137.0 3 1.0 4 1311.0 5 11507.0 6 13343.0 7 10292.0 8 8063.0 9 6950.0 10 5901.0 11 5248.0 12 4737.0 13 4489.0 14 4352.0 15 4366.0 16 4373.0 17 4715.0 18 5060.0 19 6136.0 20 7895.0 21 10265.0 22 13208.0 23 16096.0 24 18970.0 25 21638.0 26 24201.0 27 26363.0 28 30291.0 29 35395.0 30 44615.0 31 56423.0 32 72030.0 33 92959.0 34 121836.0 35 160369.0 36 220947.0 37 344018.0 38 1127857.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.483040639260345 19.358380902799514 13.460924845077994 26.697653612862155 2 12.814519424048196 20.192952933721596 42.25279359819567 24.73973404403453 3 17.620855701658197 25.471417810113184 29.268276868212027 27.639449620016592 4 11.099911867930794 16.85789914655665 37.895683816763096 34.14650516874946 5 12.28282896089348 39.4353352742102 35.240989244572226 13.040846520324093 6 31.543688438705995 37.25950696782081 18.236680782154195 12.960123811318999 7 28.38892414688477 31.58729292499867 21.68574096681877 18.338041961297787 8 25.129352890251592 38.08104829924939 20.04902074873394 16.740578061765074 9 27.180717707893503 14.698925875450986 19.922014308692283 38.19834210796323 10 15.218513469741868 29.539349364025824 33.88974158529807 21.352395580934243 11 34.340227062112206 22.237290203989797 24.916920589278426 18.505562144619574 12 23.842193512615207 24.91195877287213 31.693545219537732 19.55230249497493 13 29.347382968751372 22.179048572072517 25.71272433698532 22.760844122190793 14 20.578927237692543 22.829633002368503 27.644925339382908 28.946514420556046 15 22.66104654222759 32.23890410624537 23.070237062767973 22.029812288759064 16 21.39854683357385 28.19605848056699 28.75361918680038 21.651775499058783 17 20.870073578370842 29.23537070243052 28.164529687999334 21.730026031199305 18 20.721595780057783 27.450408356901185 31.02196767924534 20.806028183795693 19 23.2640739986531 26.684025354667128 27.92647573635234 22.12542491032743 20 23.829482485567187 27.118741665309727 28.58118546037235 20.470590388750736 21 22.81620780203345 26.546041668096688 27.268868820314058 23.368881709555808 22 23.156171086761375 28.616328457539105 27.343306098136704 20.88419435756282 23 21.730679690065084 28.1675225760642 28.301371628653154 21.800426105217557 24 23.053459159069494 26.798868346300676 28.991056519001802 21.156615975628032 25 22.07324253457578 27.66264382105252 28.754410156363207 21.509703488008494 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 81.0 1 81.0 2 280.5 3 480.0 4 480.0 5 480.0 6 1258.5 7 2037.0 8 2037.0 9 2037.0 10 2753.0 11 3469.0 12 3469.0 13 3469.0 14 5830.0 15 8191.0 16 8191.0 17 8191.0 18 16492.5 19 24794.0 20 24794.0 21 24794.0 22 42936.5 23 61079.0 24 61079.0 25 61079.0 26 93927.5 27 126776.0 28 126776.0 29 126776.0 30 168038.0 31 209300.0 32 209300.0 33 209300.0 34 236917.5 35 264535.0 36 264535.0 37 264535.0 38 285131.0 39 305727.0 40 305727.0 41 305727.0 42 321180.5 43 336634.0 44 336634.0 45 336634.0 46 349468.5 47 362303.0 48 362303.0 49 362303.0 50 343668.0 51 325033.0 52 325033.0 53 325033.0 54 280847.5 55 236662.0 56 236662.0 57 236662.0 58 199673.0 59 162684.0 60 162684.0 61 162684.0 62 132310.5 63 101937.0 64 101937.0 65 101937.0 66 76954.5 67 51972.0 68 51972.0 69 51972.0 70 37969.5 71 23967.0 72 23967.0 73 23967.0 74 16871.0 75 9775.0 76 9775.0 77 9775.0 78 6613.5 79 3452.0 80 3452.0 81 3452.0 82 2297.0 83 1142.0 84 1142.0 85 1142.0 86 709.0 87 276.0 88 276.0 89 276.0 90 161.0 91 46.0 92 46.0 93 46.0 94 24.0 95 2.0 96 2.0 97 2.0 98 2.5 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0888132317605879 2 0.04179446200498254 3 0.02028709287103167 4 0.005948846781731091 5 0.01975322200600452 6 0.06082314497987879 7 0.07588593010028764 8 0.054378560966336774 9 0.022651378130437615 10 0.04804837785244343 11 0.006368316747109566 12 2.2880179929734966E-4 13 0.004957372318109243 14 9.152071971893986E-4 15 0.013384905258894957 16 0.008427532940785713 17 0.004537902352730769 18 0.005376842283487717 19 0.0014872116954327728 20 0.001296543529351648 21 5.720044982433742E-4 22 5.720044982433742E-4 23 0.0 24 0.0 25 3.813363321622495E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2622357.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.07887769011598 #Duplication Level Percentage of deduplicated Percentage of total 1 74.27128763459113 40.165078798784776 2 14.37093501721648 15.543280741773097 3 5.010417041349432 8.128731910668261 4 2.2985986985521354 4.972225515106428 5 1.223823002745577 3.3091487239914263 6 0.7128516714804601 2.3130131011911925 7 0.4634576447660415 1.7544288500096386 8 0.31255398851547755 1.352205513318914 9 0.2214250679747915 1.077697725168093 >10 1.0065330900127976 9.413650641221905 >50 0.0589537175232264 2.2029461712991543 >100 0.0420104204137351 4.377912223405344 >500 0.00396602249588496 1.4600592945432298 >1k 0.0026912295507627686 2.3209971488432313 >5k 4.957528119826153E-4 1.6086236406753118 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7580 0.28905293977898505 No Hit GTCCTACAGTGGACATTTCTAAATT 7243 0.2762019053851173 No Hit CTGTAGGACGTGGAATATGGCAAGA 5804 0.22132760718696956 No Hit GTCCTACAGTGTGCATTTCTCATTT 5565 0.21221366884829185 No Hit GTCCTAAAGTGTGTATTTCTCATTT 5390 0.20554028303545246 No Hit CTTTAGGACGTGAAATATGGCGAGG 5322 0.20294719597674918 No Hit TATCAACGCAGAGTACTTTTTTTTT 5097 0.19436712850309854 No Hit GGTATCAACGCAGAGTACTTTTTTT 4212 0.16061886310673948 No Hit CTGTAGGACCTGGAATATGGCGAGA 3924 0.14963637674046668 No Hit CTGAAGGACCTGGAATATGGCGAGA 2785 0.10620216850718647 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.813363321622495E-5 0.0 0.0 0.0 0.0 7 3.813363321622495E-5 0.0 0.0 0.0 0.0 8 3.813363321622495E-5 0.0 0.0 0.0 0.0 9 3.813363321622495E-5 0.0 0.0 3.813363321622495E-5 0.0 10 3.813363321622495E-5 0.0 0.0 3.813363321622495E-5 0.0 11 3.813363321622495E-5 0.0 0.0 3.813363321622495E-5 0.0 12 3.813363321622495E-5 0.0 0.0 3.813363321622495E-5 0.0 13 3.813363321622495E-5 0.0 0.0 3.813363321622495E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTATACG 45 6.714356E-4 14.792293 4 CGTCTAG 40 0.0052368008 14.267812 1 TGCGCAT 65 5.4675722E-5 13.15069 10 TAGGACC 1540 0.0 12.90552 4 TCCAACG 305 0.0 12.760367 18 GGTATCA 1910 0.0 12.400298 1 CCAACGT 135 3.765308E-10 11.953023 19 GGCGAGG 1440 0.0 11.865132 19 TGTAGGA 3815 0.0 11.742017 2 AGGACCT 2545 0.0 11.508346 5 GGACCTG 2380 0.0 11.347053 6 GTAGGAC 3800 0.0 11.237742 3 CTGTAGG 3785 0.0 11.233311 1 CAACGAA 85 5.3690557E-5 11.16761 17 TGGCGAG 3470 0.0 11.133817 18 GTATCAA 4190 0.0 11.010165 1 GTCGAGG 190 0.0 10.99086 19 TACACGG 225 0.0 10.988561 5 GTCCTAC 3980 0.0 10.969726 1 CACCTTT 890 0.0 10.772762 14 >>END_MODULE