FastQCFastQC Report
Thu 26 May 2016
SRR1512878_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512878_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283237
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79710.34910961936934276No Hit
GTCCTAAAGTGTGTATTTCTCATTT64540.2826688600438763No Hit
GTATCAACGCAGAGTACTTTTTTTT60170.26352936642144464No Hit
CTGTAGGACGTGGAATATGGCAAGA58320.2554268347963877No Hit
GTCCTACAGTGTGCATTTCTCATTT56700.24833164494093254No Hit
CTTTAGGACGTGAAATATGGCGAGG47850.20957088554539016No Hit
CTGTAGGACCTGGAATATGGCGAGA37220.16301417680249575No Hit
TATCAACGCAGAGTACTTTTTTTTT35370.15491164517743886No Hit
GGTATCAACGCAGAGTACTTTTTTT33880.14838582240914983No Hit
GTACTGGTTCACTATCGGTCAGTCA30840.1350713920631104No Hit
GTACATGGGAAGCAGTGGTATCAAC24630.10787316428386541No Hit
CTGAAGGACCTGGAATATGGCGAGA24040.10528911365749591No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTCCG400.005286169714.24753410
TAGGACC16150.013.4094434
CGAGTCG550.00307267812.08908219
CGACCTC550.00307267812.08908219
CTGCGCA1103.8260623E-812.0888179
AGGACCT26200.011.7823115
TGTACGA658.037609E-411.6902842
GGACCTG26000.011.2526386
GTCCTAA19150.011.2361521
TGTAGGA39650.011.0674412
ATTTAGA8650.011.006821
ACCTTTT9100.010.856215515
TTACACA3150.010.8552644
CTGTAGG39650.010.78154851
CGATAAG1502.1373125E-910.76598312
TAGAAAT9050.010.7053284
GTAGGAC40800.010.662373
CACCTTT9450.010.65517514
CCGAATG1701.382432E-1010.6167418
TTTAGAA10100.010.4387872
Produced by FastQC (version 0.11.5)