FastQCFastQC Report
Thu 26 May 2016
SRR1512878_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512878_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283237
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74740.327342277652298No Hit
GTCCTACAGTGGACATTTCTAAATT73250.32081645488400895No Hit
GTCCTAAAGTGTGTATTTCTCATTT60770.26615721451605767No Hit
CTGTAGGACGTGGAATATGGCAAGA58610.2566969613754507No Hit
GTCCTACAGTGTGCATTTCTCATTT54600.23913417660978692No Hit
CTTTAGGACGTGAAATATGGCGAGG53090.23252075890501075No Hit
TATCAACGCAGAGTACTTTTTTTTT51820.22695848043807978No Hit
GGTATCAACGCAGAGTACTTTTTTT41680.18254784763911938No Hit
CTGTAGGACCTGGAATATGGCGAGA37870.16586101223832656No Hit
GTACTGGTTCACTATCGGTCAGTCA27720.12140658197112258No Hit
ACGCAGAGTACTTTTTTTTTTTTTT26320.11527493641702548No Hit
CTGAAGGACCTGGAATATGGCGAGA25610.11216531617173338No Hit
ATTTAGAAATGTCCACTGTAGGACG23370.10235468328517801No Hit
GTACATGGGAAGCAGTGGTATCAAC23120.10125974657908925No Hit
CCCATGTACTCTGCGTTGATACCAC22980.10064658202367954No Hit
TTTCTAAATTTTCCACCTTTTTCAG22860.10012101240475693No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCC400.00524151214.2657663
TAGGACC14250.013.0802624
CCCTATA1301.9463187E-1012.4370942
ACCGATC550.003068486212.0912878
TAGAAAT7650.011.809664
CCTATAC1950.011.7052433
TCTATAC1751.8189894E-1211.4126123
AGGACCT22000.011.4121115
ATTTAGA8200.011.1340111
AACCGTA600.005848936711.0904857
GGCGAGG14400.011.07421219
GGACCGT951.3452611E-511.0114456
CACCTTT9800.010.84939614
TTTAGAA9050.010.8243542
TGTAGGA39700.010.7804122
GGACCTG22850.010.7792566
CTAGGAC3550.010.7160673
GTGCGCC1605.511538E-1010.68057611
AATGTCC8650.010.6535118
AATCCCG1252.2669337E-710.63124419
Produced by FastQC (version 0.11.5)