##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512878_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2283237 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.47615118360468 31.0 31.0 34.0 27.0 34.0 2 30.465899510212914 31.0 30.0 34.0 27.0 34.0 3 30.65650302618607 31.0 31.0 34.0 27.0 34.0 4 34.27000482210126 37.0 35.0 37.0 30.0 37.0 5 33.90125948379428 37.0 35.0 37.0 30.0 37.0 6 33.794663015709716 37.0 35.0 37.0 29.0 37.0 7 33.63579470725115 37.0 35.0 37.0 28.0 37.0 8 33.58322154029564 37.0 35.0 37.0 28.0 37.0 9 34.94959042797572 39.0 35.0 39.0 28.0 39.0 10 34.8535977649276 39.0 35.0 39.0 27.0 39.0 11 34.86699847628608 39.0 35.0 39.0 27.0 39.0 12 34.74426001330567 39.0 35.0 39.0 27.0 39.0 13 34.73972390951969 39.0 35.0 39.0 27.0 39.0 14 35.8410528560986 40.0 36.0 41.0 27.0 41.0 15 35.846674699122346 40.0 36.0 41.0 27.0 41.0 16 35.85010666873391 40.0 36.0 41.0 27.0 41.0 17 35.836141408009766 40.0 36.0 41.0 27.0 41.0 18 35.76337366642184 40.0 36.0 41.0 27.0 41.0 19 35.7642973550271 40.0 36.0 41.0 27.0 41.0 20 35.7484159550673 40.0 36.0 41.0 27.0 41.0 21 35.71027055010058 40.0 36.0 41.0 27.0 41.0 22 35.676775122337276 40.0 36.0 41.0 27.0 41.0 23 35.52678149486891 40.0 36.0 41.0 27.0 41.0 24 35.495725148112086 40.0 36.0 41.0 27.0 41.0 25 35.427485188791174 40.0 36.0 41.0 26.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6663173450677213 1101 2 0.5774702128188522 1101 3 0.5084507324000711 1101 4 0.552994938513713 1101 5 0.6287085327039321 1101 6 0.6533325031694801 1101 7 0.7812304043791869 1101 8 0.6587285487298402 1101 9 0.8547148432654268 1101 10 0.5719845030849768 1101 11 0.8723632587139463 1101 12 1.022326516880618 1101 13 1.1033391587166932 1101 14 1.226492303376176 1101 15 0.7430345192190515 1101 16 1.1269630028617215 1101 17 1.1570635794059 1101 18 1.0286413932211858 1101 19 0.9155119363317539 1101 20 1.0306631625294642 1101 21 1.1410923172603304 1101 22 1.0715963372168744 1101 23 1.2397930470691279 1101 24 1.1484986850264889 1101 25 1.2026147246958558 1102 1 0.8253634075763401 1102 2 0.9364139318420435 1102 3 0.5954675177030033 1102 4 0.7325689025345383 1102 5 0.8210846830948881 1102 6 0.8776485937531788 1102 7 0.8685900048973139 1102 8 1.0223027785538719 1102 9 1.1931982679146529 1102 10 1.063052073760069 1102 11 1.198360562291505 1102 12 1.1655151979193192 1102 13 1.2865625205984372 1102 14 1.2864532053278666 1102 15 1.3590604328732496 1102 16 1.2897541308008655 1102 17 1.390461767796701 1102 18 0.6871429368918172 1102 19 1.030373611895655 1102 20 1.2192905472132338 1102 21 1.2323635727480138 1102 22 1.001326233209035 1102 23 1.2538248816285247 1102 24 1.2070752209708644 1102 25 1.271042090924496 1103 1 0.68778941895917 1103 2 0.7804870862758406 1103 3 0.5568716712824973 1103 4 0.40117947898942674 1103 5 0.526583621685738 1103 6 0.5535197742397102 1103 7 0.6536781001364815 1103 8 0.6858511117503312 1103 9 0.780417663153024 1103 10 0.66973068660878 1103 11 0.8370907995203041 1103 12 0.9655916678449614 1103 13 0.8994954518962217 1103 14 0.8277779301101802 1103 15 0.9700685269875038 1103 16 0.8605511906578798 1103 17 0.8394912414780791 1103 18 0.8785466970520943 1103 19 0.9310165606190708 1103 20 0.8004637771745493 1103 21 0.9061733412424999 1103 22 0.7733913295795389 1103 23 0.9068120030578939 1103 24 1.0184260684901005 1103 25 0.9087173513328608 1104 1 0.8701217686863849 1104 2 0.9300344291601554 1104 3 0.8172664436925636 1104 4 0.7378905011182582 1104 5 0.810945089553492 1104 6 0.7579127377806785 1104 7 0.7772816202446435 1104 8 0.8749811086356516 1104 9 0.9705235875571461 1104 10 1.0179545372903007 1104 11 1.0553173921324799 1104 12 1.0459549863295265 1104 13 0.8998880295689702 1104 14 1.1366498574965789 1104 15 1.1978200111562458 1104 16 1.1707782788751402 1104 17 1.0528138188093905 1104 18 0.6146749359110757 1104 19 1.081545124036765 1104 20 0.9851574823488676 1104 21 0.9750954732449131 1104 22 0.8702024299406119 1104 23 0.920091132939973 1104 24 1.0974780612504489 1104 25 0.920658690417838 1105 1 0.6224478760849586 1105 2 0.6470149142565838 1105 3 0.6743883877710282 1105 4 0.46179094965638257 1105 5 0.45477139280008316 1105 6 0.6169259011416699 1105 7 0.6809514297115555 1105 8 0.6305970671501626 1105 9 0.8933838654898878 1105 10 0.49308381676740964 1105 11 0.7426991084996999 1105 12 0.9198948022983231 1105 13 0.8842063399742415 1105 14 0.9854439744066354 1105 15 1.0034115051535366 1105 16 0.953454235464136 1105 17 0.7897431064633196 1105 18 0.7161782837912369 1105 19 0.9607299742361093 1105 20 1.043113989580732 1105 21 0.8292955230971373 1105 22 0.8197197997478369 1105 23 1.0203527911726482 1105 24 0.9108889688642563 1105 25 0.8570351195478949 1106 1 0.16016718600453217 1106 2 0.44843712938103764 1106 3 -1.434983326739026 1106 4 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1107 17 0.8155837657898672 1107 18 0.8713095609667576 1107 19 0.8591432963717835 1107 20 0.8444042889640926 1107 21 0.8062735412650852 1107 22 0.8594907613342073 1107 23 0.9435332353294399 1107 24 0.9572205788877071 1107 25 0.9775936157738201 1108 1 0.614285359227555 1108 2 0.5142337912548136 1108 3 0.5529388739710193 1108 4 0.43388621800239235 1108 5 0.6466065767221139 1108 6 0.6405031306830722 1108 7 0.5696387819830875 1108 8 0.5205897238370767 1108 9 0.7798783006664891 1108 10 0.7253297890864943 1108 11 0.731242319511658 1108 12 0.768738223390919 1108 13 0.8322192598906852 1108 14 0.9829192315290385 1108 15 0.7615657192700169 1108 16 0.794823770451039 1108 17 0.8342220959871014 1108 18 0.9069783926777788 1108 19 0.9049286007782555 1108 20 0.9728500929922959 1108 21 0.9009941617030961 1108 22 0.9574969019521689 1108 23 0.976873962594297 1108 24 0.8833372101450294 1108 25 0.9491549526528615 1109 1 0.28719612381383897 1109 2 0.09238261805701953 1109 3 0.3137355596307039 1109 4 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2216 15 -3.0284315518300104 2216 16 -2.4653068852712536 2216 17 -2.911511492454693 2216 18 -3.2321837502333253 2216 19 -3.467476631617643 2216 20 -2.758330903996665 2216 21 -3.414855967726936 2216 22 -2.915128222736975 2216 23 -4.0934990872829005 2216 24 -3.1794305728010386 2216 25 -3.319909766987351 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 52996.0 3 3.0 4 897.0 5 7452.0 6 9392.0 7 7453.0 8 5977.0 9 4975.0 10 4425.0 11 3892.0 12 3595.0 13 3319.0 14 3391.0 15 3186.0 16 3457.0 17 3678.0 18 4121.0 19 5017.0 20 6624.0 21 8767.0 22 11172.0 23 13767.0 24 16218.0 25 18611.0 26 21111.0 27 23049.0 28 26330.0 29 31693.0 30 39495.0 31 50588.0 32 64470.0 33 83909.0 34 108506.0 35 142917.0 36 196003.0 37 304709.0 38 988072.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.07934111524346 19.038895522859143 13.130263704701234 26.751499657196163 2 13.53931063008652 19.983805511909697 41.079317062312846 25.397566795690935 3 17.894936041797443 25.25202392305303 29.07151202088295 27.78152801426657 4 11.779371011774641 16.482306941313382 36.82068496408654 34.91763708282544 5 12.855716676866036 38.89779356331486 34.660317212781905 13.586172547037195 6 32.37909836694214 36.17957457140065 17.915998515202737 13.525328546454476 7 29.027648719252415 31.268650174448165 21.18975401932079 18.51394708697863 8 26.016447557252476 37.097580630672276 19.797015040483497 17.088956771591747 9 27.733442209437264 14.087335399000404 19.5660697318152 38.613152659747136 10 16.194699921169686 28.58140051539864 33.14312982200299 22.080769741428682 11 34.85998758715599 21.59996916498877 24.630560211710385 18.909483036144852 12 24.95576446020378 24.14634167706129 30.845266709646673 20.052627153088252 13 30.191748565762076 21.661920183750645 25.225855790431755 22.92047546005552 14 21.29459102330346 22.420017668007024 26.684778490756266 29.600612817933246 15 23.384173571982693 31.824129189895494 22.423729786153856 22.367967451967953 16 22.085059487151636 27.192562113066327 28.220151080983296 22.502227318798745 17 21.813699940520355 28.646763615276193 27.433913208773554 22.105623235429896 18 21.65542464193421 26.21492707283956 30.72559239630327 21.404055888922954 19 24.36966729575477 25.85709787013747 27.120281674965696 22.652953159142065 20 25.071533029579836 26.179490200423878 27.747939196158395 21.0010375738379 21 23.526477333048355 25.678072700325327 26.666187814137476 24.129262152488838 22 24.473881299737652 27.877699574725277 26.385296268882243 21.263122856654824 23 22.24105513356695 27.56726524666515 27.991837903818134 22.199841715949766 24 24.178085761574465 25.922889301461037 28.19794878937228 21.701076147592214 25 22.788236865675238 27.016886128672695 28.091501750805325 22.103375254846743 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 97.0 1 97.0 2 317.0 3 537.0 4 537.0 5 537.0 6 1277.0 7 2017.0 8 2017.0 9 2017.0 10 2352.0 11 2687.0 12 2687.0 13 2687.0 14 4065.5 15 5444.0 16 5444.0 17 5444.0 18 11554.0 19 17664.0 20 17664.0 21 17664.0 22 31767.5 23 45871.0 24 45871.0 25 45871.0 26 73146.5 27 100422.0 28 100422.0 29 100422.0 30 132395.0 31 164368.0 32 164368.0 33 164368.0 34 186881.5 35 209395.0 36 209395.0 37 209395.0 38 224513.5 39 239632.0 40 239632.0 41 239632.0 42 256337.0 43 273042.0 44 273042.0 45 273042.0 46 294593.5 47 316145.0 48 316145.0 49 316145.0 50 305473.0 51 294801.0 52 294801.0 53 294801.0 54 260097.5 55 225394.0 56 225394.0 57 225394.0 58 195380.0 59 165366.0 60 165366.0 61 165366.0 62 138305.0 63 111244.0 64 111244.0 65 111244.0 66 85649.5 67 60055.0 68 60055.0 69 60055.0 70 44904.5 71 29754.0 72 29754.0 73 29754.0 74 21003.5 75 12253.0 76 12253.0 77 12253.0 78 8512.5 79 4772.0 80 4772.0 81 4772.0 82 3220.5 83 1669.0 84 1669.0 85 1669.0 86 1088.5 87 508.0 88 508.0 89 508.0 90 301.0 91 94.0 92 94.0 93 94.0 94 50.0 95 6.0 96 6.0 97 6.0 98 3.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08974101243103541 2 0.04287772141043615 3 0.0183073417258042 4 0.004817721506790578 5 0.018219746789317096 6 0.06324354414368723 7 0.0769521517039186 8 0.05238177201928665 9 0.022774683486646373 10 0.0490531644327768 11 0.005168101252738984 12 2.1898734121775357E-4 13 0.006263037958827752 14 6.1316455540971E-4 15 0.014628354393345937 16 0.00976683541831181 17 0.004511139229085723 18 0.006000253149366448 19 0.0016643037932549272 20 0.0011387341743323185 21 4.379746824355071E-5 22 3.06582277704855E-4 23 0.0 24 0.0 25 8.759493648710142E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2283237.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.809190880771105 #Duplication Level Percentage of deduplicated Percentage of total 1 70.98160563394156 33.93573132776729 2 15.1037462720071 14.441957770662448 3 5.836695343420691 8.37143045359523 4 2.6592126952980735 5.085392293683015 5 1.5242886946343428 3.643750457958736 6 0.9301452331587396 2.6681694599355312 7 0.6341022632830114 2.1221141300258526 8 0.42519637896584284 1.6262635875032534 9 0.3154323007883489 1.357250677051588 >10 1.4436516117041118 11.990421920545916 >50 0.08063394625000503 2.631623020427647 >100 0.05418360214778793 5.0401655332677135 >500 0.0064249727936497225 2.0796034040291036 >1k 0.004038554327436836 3.132898766163409 >5k 6.424972793649511E-4 1.8732271973833041 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7474 0.327342277652298 No Hit GTCCTACAGTGGACATTTCTAAATT 7325 0.32081645488400895 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6077 0.26615721451605767 No Hit CTGTAGGACGTGGAATATGGCAAGA 5861 0.2566969613754507 No Hit GTCCTACAGTGTGCATTTCTCATTT 5460 0.23913417660978692 No Hit CTTTAGGACGTGAAATATGGCGAGG 5309 0.23252075890501075 No Hit TATCAACGCAGAGTACTTTTTTTTT 5182 0.22695848043807978 No Hit GGTATCAACGCAGAGTACTTTTTTT 4168 0.18254784763911938 No Hit CTGTAGGACCTGGAATATGGCGAGA 3787 0.16586101223832656 No Hit GTACTGGTTCACTATCGGTCAGTCA 2772 0.12140658197112258 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2632 0.11527493641702548 No Hit CTGAAGGACCTGGAATATGGCGAGA 2561 0.11216531617173338 No Hit ATTTAGAAATGTCCACTGTAGGACG 2337 0.10235468328517801 No Hit GTACATGGGAAGCAGTGGTATCAAC 2312 0.10125974657908925 No Hit CCCATGTACTCTGCGTTGATACCAC 2298 0.10064658202367954 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2286 0.10012101240475693 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 4.379746824355071E-5 13 0.0 0.0 0.0 0.0 4.379746824355071E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTACGCC 40 0.005241512 14.265766 3 TAGGACC 1425 0.0 13.080262 4 CCCTATA 130 1.9463187E-10 12.437094 2 ACCGATC 55 0.0030684862 12.091287 8 TAGAAAT 765 0.0 11.80966 4 CCTATAC 195 0.0 11.705243 3 TCTATAC 175 1.8189894E-12 11.412612 3 AGGACCT 2200 0.0 11.412111 5 ATTTAGA 820 0.0 11.134011 1 AACCGTA 60 0.0058489367 11.090485 7 GGCGAGG 1440 0.0 11.074212 19 GGACCGT 95 1.3452611E-5 11.011445 6 CACCTTT 980 0.0 10.849396 14 TTTAGAA 905 0.0 10.824354 2 TGTAGGA 3970 0.0 10.780412 2 GGACCTG 2285 0.0 10.779256 6 CTAGGAC 355 0.0 10.716067 3 GTGCGCC 160 5.511538E-10 10.680576 11 AATGTCC 865 0.0 10.653511 8 AATCCCG 125 2.2669337E-7 10.631244 19 >>END_MODULE