FastQCFastQC Report
Thu 26 May 2016
SRR1512877_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512877_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229225
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT65350.2931512072581278No Hit
GGTATCAACGCAGAGTACTTTTTTT36300.16283686034384148No Hit
TATCAACGCAGAGTACTTTTTTTTT35270.158216420504884No Hit
GTACTGGTTCACTATCGGTCAGTCA31470.14117013760387578No Hit
GTCCTACAGTGGACATTTCTAAATT31470.14117013760387578No Hit
CTGTAGGACGTGGAATATGGCAAGA28450.1276228285614956No Hit
GTCCTAAAGTGTGTATTTCTCATTT24060.10792988594690979No Hit
GGATACCACGTGTCCCGCCCTACTC22320.10012448272381656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTTT1207.421477E-1012.6648112
TCCAACG2000.012.34846718
TAGGACC5150.012.1732464
TAGCGTG550.003072017312.0894085
GCCGGTT1201.00426405E-811.8732611
GGTATCA17850.011.6297871
CCAACGA1751.8189894E-1211.39832919
TATACTG3050.011.2117235
GTCCTAG3000.011.1096491
TTAGGAC8150.010.9556963
GTATCAA38500.010.9323881
GTCCTAC14000.010.882921
TTATACT2450.010.8557964
CTACACG1408.372808E-910.8557954
GTAGGAC13850.010.83623
GTATAAT4550.010.4643471
TGGACCG1002.4029418E-510.4487035
GTAATAC1106.056818E-610.36234953
GTCCTAT2300.010.3506041
GTCTAGG2300.010.3506041
Produced by FastQC (version 0.11.5)