FastQCFastQC Report
Thu 26 May 2016
SRR1512877_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512877_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2229225
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT77000.3454115219414819No Hit
TATCAACGCAGAGTACTTTTTTTTT51480.2309322746694479No Hit
GGTATCAACGCAGAGTACTTTTTTT46250.20747120636095506No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29070.13040406419271272No Hit
GTCCTACAGTGGACATTTCTAAATT29030.13022462963585998No Hit
GTACTGGTTCACTATCGGTCAGTCA28860.12946203276923593No Hit
CTGTAGGACGTGGAATATGGCAAGA27580.12372012694994898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19050.012.9828941
CCAACGA2050.012.50079219
CCCCGTA3050.012.44905915
CTAATAC1603.6379788E-1211.8895123
TCCAACG2550.011.53875318
CCCGTAC3350.011.33421716
CCGTACA3400.011.16678517
TAGGACC5550.011.1397244
TACCGTG1053.4405093E-610.8643067
GCGCCGA700.001501265310.8489511
GTCCTAA6250.010.8061621
ATTTAGA3700.010.7979351
ATACCGT803.7304964E-410.7003216
TAATACT2350.010.5234834
TTAGGAC9400.010.4222973
TTACCCT1855.820766E-1110.2828224
GTAGGAC14000.010.2589513
TTTAGAA4550.010.2432742
TATACAC6600.010.2321873
CGGTTTC1303.885525E-710.22259113
Produced by FastQC (version 0.11.5)