##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512877_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2229225
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.10231986452691	31.0	30.0	34.0	26.0	34.0
2	30.11518128497572	31.0	30.0	34.0	26.0	34.0
3	30.31741434803575	31.0	30.0	34.0	26.0	34.0
4	33.913090423802	37.0	35.0	37.0	30.0	37.0
5	33.453717323285	37.0	35.0	37.0	28.0	37.0
6	33.30911191109018	37.0	35.0	37.0	27.0	37.0
7	33.15158586504278	37.0	35.0	37.0	26.0	37.0
8	33.096025300272515	37.0	35.0	37.0	26.0	37.0
9	34.41215041101728	39.0	35.0	39.0	26.0	39.0
10	34.339768305128466	39.0	35.0	39.0	26.0	39.0
11	34.32221108232682	39.0	35.0	39.0	25.0	39.0
12	34.16207336630443	39.0	35.0	39.0	25.0	39.0
13	34.157993024481605	39.0	35.0	39.0	25.0	39.0
14	35.244009913759264	40.0	35.0	41.0	25.0	41.0
15	35.226931781224415	40.0	35.0	41.0	25.0	41.0
16	35.22095391896287	40.0	35.0	41.0	25.0	41.0
17	35.19959582366069	40.0	35.0	41.0	25.0	41.0
18	35.11735917190952	40.0	35.0	41.0	25.0	41.0
19	35.12355908443517	40.0	35.0	41.0	24.0	41.0
20	35.08767710751494	40.0	35.0	41.0	24.0	41.0
21	35.06066009487602	40.0	35.0	41.0	24.0	41.0
22	35.01872937904429	40.0	35.0	41.0	24.0	41.0
23	34.85419686213819	40.0	35.0	41.0	22.0	41.0
24	34.801081990377824	40.0	35.0	41.0	21.0	41.0
25	34.742089739707744	40.0	35.0	41.0	20.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.6581500047406621
1101	2	0.607473680551692
1101	3	0.587076157778867
1101	4	0.5771837926432539
1101	5	0.6508711920713992
1101	6	0.6326729597609599
1101	7	0.7149336774342459
1101	8	0.4564993771726975
1101	9	0.8177708689933283
1101	10	0.43706912798367625
1101	11	0.7460728531811256
1101	12	0.8512885885872308
1101	13	0.9611225647860948
1101	14	1.063705964760402
1101	15	0.6640024394276125
1101	16	0.8892496599062625
1101	17	0.9802123627771167
1101	18	0.8254206901828738
1101	19	0.6473436740359233
1101	20	0.8436281974928406
1101	21	0.8497739092716401
1101	22	0.8661076094249793
1101	23	0.9407153265312544
1101	24	0.9089958961242033
1101	25	1.169330885425488
1102	1	0.7778877251593315
1102	2	0.9015013402490375
1102	3	0.514675550162714
1102	4	0.6524102720287601
1102	5	0.869691981569467
1102	6	0.7419052601199709
1102	7	0.6955901553826394
1102	8	0.7889618477421294
1102	9	0.8768506609210576
1102	10	0.8727231950766381
1102	11	0.9319539704749502
1102	12	1.0222648440116515
1102	13	1.005088777889597
1102	14	0.9710649162867568
1102	15	1.0242086850877428
1102	16	1.0300433484344893
1102	17	1.2459407433135254
1102	18	0.49217438940213043
1102	19	0.8502490570261614
1102	20	0.9964210962812317
1102	21	0.9492043027044446
1102	22	0.702962940638912
1102	23	0.9356185498248166
1102	24	0.9242883751954238
1102	25	1.0336186623213024
1103	1	0.657010589105262
1103	2	0.8710502084314342
1103	3	0.5477827940599447
1103	4	0.48128281556820696
1103	5	0.6206225934946303
1103	6	0.6508431550837059
1103	7	0.7862250517670333
1103	8	0.8393725551247826
1103	9	0.9693859052025289
1103	10	0.856358968099066
1103	11	0.992620798133828
1103	12	1.0731021548123039
1103	13	1.0542190697843807
1103	14	1.1631076990587275
1103	15	1.1981487498149122
1103	16	1.2000748992762524
1103	17	1.127110075905705
1103	18	1.1940897038682508
1103	19	1.2127783535879075
1103	20	1.0719545429316426
1103	21	1.2160756940515185
1103	22	1.144155866976675
1103	23	1.2414123585762766
1103	24	1.1701507154428654
1103	25	1.0968248783844388
1104	1	0.9149116730832105
1104	2	0.9506646161139507
1104	3	0.8692725085413926
1104	4	0.7635358120835107
1104	5	0.9686557257566619
1104	6	0.9246960136406344
1104	7	0.855913755362856
1104	8	0.9449912910322595
1104	9	0.9996425172252685
1104	10	1.0444149990243972
1104	11	1.1404429302717887
1104	12	1.1543025612703204
1104	13	0.9801674156324154
1104	14	1.27409592202536
1104	15	1.2309092288527879
1104	16	1.1865216349355876
1104	17	1.1492779003169176
1104	18	0.7486746285103436
1104	19	1.2039545128158764
1104	20	1.146172660790981
1104	21	1.1894103995515195
1104	22	1.0632716994199498
1104	23	1.0509738316427004
1104	24	1.3350095441047642
1104	25	1.2012386699068074
1105	1	0.7021098236405336
1105	2	0.7265479297723623
1105	3	0.6745444842247963
1105	4	0.6548077757210393
1105	5	0.6037147806376524
1105	6	0.8088345632374967
1105	7	0.8368044872699585
1105	8	0.8015440439152357
1105	9	1.0437821007593655
1105	10	0.6054943391516048
1105	11	1.050097896382745
1105	12	1.0452899412131416
1105	13	1.0528617356028604
1105	14	1.101013323490136
1105	15	1.1344928251876283
1105	16	1.001389150434612
1105	17	0.951197359617936
1105	18	0.7915328325513329
1105	19	0.9535695468655376
1105	20	1.082047221116703
1105	21	0.958750295876726
1105	22	0.8781462416768662
1105	23	1.1669494668616167
1105	24	1.0405525016872375
1105	25	0.8988561974516998
1106	1	0.06497997238958675
1106	2	0.3303443455341828
1106	3	-1.5293480090610139
1106	4	-0.28363569688128365
1106	5	0.01712880683243867
1106	6	0.04934122532758778
1106	7	0.2649182214013308
1106	8	0.27234127794496743
1106	9	0.5819227325112237
1106	10	0.16176813506680077
1106	11	0.35777786679842194
1106	12	0.39208025725251616
1106	13	0.4168596843145238
1106	14	0.5063851348247255
1106	15	0.5857640008067193
1106	16	0.5944913869314021
1106	17	0.6193049337549539
1106	18	0.5237245238120067
1106	19	0.4974268168336806
1106	20	0.5593640379013038
1106	21	0.5227631593342679
1106	22	0.5385305824195612
1106	23	0.5718235240913359
1106	24	0.5981244423510645
1106	25	0.5704434533121159
1107	1	0.6861970222479812
1107	2	0.8162742024228251
1107	3	0.64442703526149
1107	4	0.5655135721899569
1107	5	0.5649333924389239
1107	6	0.5010989518897162
1107	7	0.5228995780154264
1107	8	0.5889532171037146
1107	9	0.6423250582071702
1107	10	0.6389287807158581
1107	11	0.6100966370939673
1107	12	0.949326618972421
1107	13	0.7565836236240173
1107	14	0.8673113966565964
1107	15	0.763141953174781
1107	16	0.9302825820671146
1107	17	0.7691503711072443
1107	18	0.8701432852810598
1107	19	0.7729758522510224
1107	20	0.884627253908512
1107	21	0.7927125839103084
1107	22	0.7956752412230514
1107	23	0.962760741342187
1107	24	0.9452275869265634
1107	25	0.9290257381285514
1108	1	0.5248956976006873
1108	2	0.3599938088630381
1108	3	0.4118980301898034
1108	4	0.2732554260636846
1108	5	0.47944553405087476
1108	6	0.4959742152577462
1108	7	0.3618008836883604
1108	8	0.35547968435516
1108	9	0.5096715339180165
1108	10	0.5476735084140714
1108	11	0.4463277763855089
1108	12	0.38988778557913406
1108	13	0.5060141162062237
1108	14	0.6219724869705843
1108	15	0.664392047058314
1108	16	0.5245416531932818
1108	17	0.6288933476304308
1108	18	0.6362026671123218
1108	19	0.668492527943684
1108	20	0.6998693548441892
1108	21	0.7469493601670578
1108	22	0.7044170437078989
1108	23	0.6521018963271032
1108	24	0.6909222658053338
1108	25	0.6200695254101944
1109	1	0.24423433813957374
1109	2	0.19190460040048052
1109	3	0.3656257823972986
1109	4	0.3820640703558098
1109	5	0.033634736587274006
1109	6	0.3039986310371319
1109	7	0.2896878125546891
1109	8	0.36050704346070006
1109	9	0.44480299590170347
1109	10	0.15617794315717504
1109	11	0.4165646252486752
1109	12	0.6196473810199379
1109	13	0.5201405014461358
1109	14	0.6773212414892811
1109	15	0.6820571400002606
1109	16	0.5324135691208269
1109	17	0.6086977843489194
1109	18	0.8081296568205616
1109	19	0.7385132695038408
1109	20	0.7648469342053517
1109	21	0.9226493384378074
1109	22	0.8381163503471782
1109	23	0.8197825948238346
1109	24	0.8121078928918379
1109	25	0.7202133335606433
1110	1	0.32455177541425684
1110	2	0.1787941568608744
1110	3	0.13639692114264435
1110	4	0.23817332522269652
1110	5	0.28996525784718585
1110	6	0.25603190138564
1110	7	0.17379019294400422
1110	8	0.2573270512499022
1110	9	0.12213158078799324
1110	10	0.2577088568429886
1110	11	0.22526215057723675
1110	12	0.3870699436359857
1110	13	0.2736242959267301
1110	14	0.38876910418080257
1110	15	0.40892824588735266
1110	16	0.20929009935159115
1110	17	0.27187468585454155
1110	18	0.28160180652307787
1110	19	0.4177125895487066
1110	20	0.4702530146214414
1110	21	0.4717554924532692
1110	22	0.4711592666616511
1110	23	0.5755556964791566
1110	24	0.573674583831469
1110	25	0.5350492647253304
1111	1	0.11508752874494377
1111	2	-0.008432352376463115
1111	3	0.040103304556634356
1111	4	0.23850224141334309
1111	5	0.4281147500212299
1111	6	0.3524639349135086
1111	7	0.17312438399316932
1111	8	0.06745982828789465
1111	9	0.15368407459207845
1111	10	0.1397766839597594
1111	11	0.19830744529271271
1111	12	0.3348708034163437
1111	13	0.2180224449872341
1111	14	0.08450848994741023
1111	15	0.09820895638385707
1111	16	0.22455115370393486
1111	17	0.3132857503887436
1111	18	0.09897871632497157
1111	19	0.0078403178873927
1111	20	0.1628852919508077
1111	21	0.2931169333407837
1111	22	-0.010046058683855108
1111	23	0.0547485802530332
1111	24	0.2240160279283856
1111	25	0.17735079594515213
1112	1	0.05254473990071773
1112	2	-0.372954170703963
1112	3	-0.10334114287832463
1112	4	-0.040818383884612786
1112	5	-0.1458067390815927
1112	6	-0.10375076090633684
1112	7	-0.047463109738352216
1112	8	-0.16450760315637325
1112	9	-0.0541246941936393
1112	10	-0.4329587053417683
1112	11	-0.19010358005251504
1112	12	0.07319008700464025
1112	13	-0.04808077786928777
1112	14	-0.28326071742917946
1112	15	-0.4026233862127455
1112	16	-0.449273721166243
1112	17	-0.2838155751412046
1112	18	-0.2005198363177243
1112	19	-0.10649534093165158
1112	20	-0.18102982116951694
1112	21	-0.044389559426448955
1112	22	-0.015623419212715817
1112	23	0.14796801211927146
1112	24	0.21385838555077896
1112	25	0.13560914729117712
1113	1	-0.7476806594178278
1113	2	-0.6065301664495131
1113	3	-0.5145927120162419
1113	4	-1.9871560314454122
1113	5	-0.7982352698737998
1113	6	-0.43218925997567936
1113	7	-0.4897128051864641
1113	8	-0.3334861330210117
1113	9	-0.2415936979224611
1113	10	-0.3711807406759746
1113	11	-0.6609953760120177
1113	12	-0.1954880650301405
1113	13	-0.38727042248660126
1113	14	-0.5083986160520482
1113	15	-0.6615487663615269
1113	16	-0.9119633317726894
1113	17	-0.8103383345977733
1113	18	-0.7480820298333981
1113	19	-0.5343868833555732
1113	20	-0.27098362822275135
1113	21	-0.415247189919981
1113	22	-0.3802949360357317
1113	23	-0.17612250626824277
1113	24	-0.07517759176706562
1113	25	-0.394815741245921
1114	1	-0.9830494989334859
1114	2	-2.2288873890750978
1114	3	-1.4471747839771467
1114	4	-1.103319071632427
1114	5	-0.9791223438044909
1114	6	-0.6950447586261248
1114	7	-0.6607002896804488
1114	8	-0.6399676446492961
1114	9	-0.9979807897603621
1114	10	-1.0451114365498313
1114	11	-0.9799552769038016
1114	12	-1.0674328522437477
1114	13	-1.0894016236691328
1114	14	-1.0603517219747687
1114	15	-1.1847652904760153
1114	16	-1.5972254606459373
1114	17	-1.5760381480372914
1114	18	-1.320989491927108
1114	19	-1.7233701287265646
1114	20	-1.1614628574967938
1114	21	-1.3778390457246772
1114	22	-1.133893876580565
1114	23	-0.9583759810793211
1114	24	-1.0440463287232973
1114	25	-1.5869671775662866
1115	1	-1.0995017644293839
1115	2	-1.2460452108920563
1115	3	-0.7989476532689466
1115	4	-0.5874671903748805
1115	5	-0.8775321974086125
1115	6	-0.7937703018996061
1115	7	-0.655853639752614
1115	8	-0.9332718673927616
1115	9	-1.823503993676276
1115	10	-1.4444174048566296
1115	11	-1.6779070777884968
1115	12	-2.404948763640011
1115	13	-2.4489291013143486
1115	14	-1.9481924197712814
1115	15	-2.0624308927568507
1115	16	-1.835977281962812
1115	17	-1.832797712768965
1115	18	-2.1707816544864755
1115	19	-2.5899293635795573
1115	20	-2.731790440708231
1115	21	-2.036262949600136
1115	22	-1.9393371900734593
1115	23	-1.9063947856143812
1115	24	-2.540572623005218
1115	25	-2.434145251827772
1116	1	-1.5486055322478158
1116	2	-1.6536871198684153
1116	3	-1.7545681528931212
1116	4	-1.3628674961190157
1116	5	-1.8253099846005227
1116	6	-2.6516912659498573
1116	7	-2.018006727272173
1116	8	-2.267908587433528
1116	9	-3.352597497400037
1116	10	-2.1288996793219113
1116	11	-3.088467536090011
1116	12	-5.11548344066599
1116	13	-3.9933261523237533
1116	14	-3.5393059287965443
1116	15	-4.595064782909251
1116	16	-4.015393172669565
1116	17	-3.7659503754421557
1116	18	-3.758137209746156
1116	19	-4.0598037010431405
1116	20	-5.948041782726509
1116	21	-4.670940157136986
1116	22	-3.784137845135728
1116	23	-4.648288208484189
1116	24	-4.587289988812472
1116	25	-3.7393189970482474
1201	1	0.6034891490398309
1201	2	0.865795000296476
1201	3	0.8825443266301995
1201	4	0.8021970562005549
1201	5	0.2212119335528584
1201	6	0.8962963691757295
1201	7	1.079219848057761
1201	8	0.968363247242614
1201	9	0.8738591890075469
1201	10	0.9820924468451508
1201	11	0.880537824890169
1201	12	1.286569928775947
1201	13	1.3166692316749717
1201	14	1.2445436660400873
1201	15	1.1343120705757244
1201	16	1.055654666447765
1201	17	0.7958705824327339
1201	18	1.2151353917597802
1201	19	1.323471215291839
1201	20	1.4446542829518165
1201	21	1.0452476335396454
1201	22	1.1010345140301396
1201	23	1.1962956036909915
1201	24	1.0276970952608906
1201	25	1.2970349150469715
1202	1	0.9195693951256416
1202	2	0.990322675265336
1202	3	0.9396889504687564
1202	4	0.771873640202891
1202	5	0.9037201135668056
1202	6	0.9158328574018739
1202	7	1.0880975471643666
1202	8	0.9883210285367525
1202	9	1.339285406473742
1202	10	1.3118351042023946
1202	11	1.293411736456619
1202	12	1.3755752599398576
1202	13	1.3030897007816407
1202	14	1.3348598109142458
1202	15	1.2561614987197416
1202	16	1.3643859373447995
1202	17	1.3607154452647805
1202	18	1.387282593271216
1202	19	1.4265531733696335
1202	20	1.3003578684500923
1202	21	1.4040673350063884
1202	22	1.3058721578881318
1202	23	1.484979135490235
1202	24	1.5283050088656367
1202	25	1.548281100687099
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2211	10	-0.061803778125906206
2211	11	0.00881926164225888
2211	12	-0.23548178486991134
2211	13	0.029269746134069408
2211	14	-0.019650759604147083
2211	15	-0.09590677454200858
2211	16	-0.05750580094947111
2211	17	-0.25785775265794797
2211	18	-0.28325312352492915
2211	19	-0.2912434028452182
2211	20	-0.20517112842612306
2211	21	-0.10284175839487375
2211	22	-0.11270601319709783
2211	23	-0.22072026688675805
2211	24	-0.03002755125515222
2211	25	-0.15701763700827343
2212	1	-0.04255999783447706
2212	2	0.011174522989978897
2212	3	0.05622967284342906
2212	4	0.03349104349857157
2212	5	-0.19141994577211108
2212	6	0.1397913793614478
2212	7	-0.08926747769947951
2212	8	0.09225701981699785
2212	9	0.11592044777417954
2212	10	-0.1535352938976473
2212	11	0.014321390870122741
2212	12	0.30942786681259093
2212	13	0.37950169880367923
2212	14	0.40225579369760567
2212	15	0.14180106356770494
2212	16	0.18500803830452384
2212	17	0.1073212175760716
2212	18	-0.011330220312657957
2212	19	0.3229056061258291
2212	20	0.12240537440488453
2212	21	0.25877953778946505
2212	22	0.35728224062830805
2212	23	0.014688030059197388
2212	24	0.017909555396542487
2212	25	0.09351143944822837
2213	1	-0.510223180807376
2213	2	-0.051153787725670696
2213	3	-0.1370401401164365
2213	4	-0.3331661077262069
2213	5	-0.21832662643497258
2213	6	-0.1799936100426791
2213	7	-0.6366480438216513
2213	8	-0.6808581822942799
2213	9	-0.43501705633912024
2213	10	-0.3916754061743788
2213	11	-0.2695447523649719
2213	12	-0.48258475609291906
2213	13	-0.36239863091872593
2213	14	-0.4225943175261193
2213	15	-0.7622233102229856
2213	16	-1.0751320772628432
2213	17	-0.7900511219392783
2213	18	-0.37327788593070466
2213	19	-0.437541197343819
2213	20	-0.4469004151519371
2213	21	-0.30717843412160306
2213	22	-0.5025890294281652
2213	23	-0.5959001065156428
2213	24	-0.3890788648316956
2213	25	-0.40236522068102687
2214	1	-1.148229429976368
2214	2	-0.5471790835639894
2214	3	-0.6811250357420207
2214	4	-0.8088756210887951
2214	5	-1.1244858805235296
2214	6	-1.6771794895799772
2214	7	-1.2660578629946357
2214	8	-1.1276556493864227
2214	9	-0.8178524505686156
2214	10	-1.1580623560480632
2214	11	-1.2621637524979334
2214	12	-1.0088425675541615
2214	13	-1.3308178903257186
2214	14	-1.1310097543031432
2214	15	-1.1206824579427064
2214	16	-1.1625373425201246
2214	17	-1.2790348009713242
2214	18	-1.2305987209260536
2214	19	-1.1971576047364536
2214	20	-1.4681404430737572
2214	21	-1.6367616012536388
2214	22	-2.0831926725607275
2214	23	-2.040712963848442
2214	24	-1.8046850009838025
2214	25	-2.30865062155857
2215	1	-1.6700707482478947
2215	2	-1.1439838975312746
2215	3	-1.0074723048810519
2215	4	-1.1997230294648062
2215	5	-1.5824116127284356
2215	6	-1.5279394813468343
2215	7	-2.0369999525179594
2215	8	-1.736234342496008
2215	9	-1.791349720313022
2215	10	-1.8024787109449676
2215	11	-1.5648358917833463
2215	12	-1.7597490608405124
2215	13	-1.8886060806081915
2215	14	-2.0459185238114586
2215	15	-1.8798385607601347
2215	16	-1.8657749050254253
2215	17	-1.9652379901035744
2215	18	-1.6781998493509818
2215	19	-1.971949657331713
2215	20	-1.8144080444443276
2215	21	-1.652527344113203
2215	22	-1.7224991513180044
2215	23	-2.322964486374566
2215	24	-2.304286177838989
2215	25	-2.367779361282281
2216	1	-1.2707880703349055
2216	2	-0.6647100771033649
2216	3	-0.7329962888704742
2216	4	-0.9859677577513821
2216	5	-1.7962058036944732
2216	6	-2.12272631456106
2216	7	-2.0723869474483934
2216	8	-2.1350929347598004
2216	9	-3.914914321239703
2216	10	-2.0640375612165656
2216	11	-2.236216485167745
2216	12	-2.478613826502997
2216	13	-2.4016752327388495
2216	14	-2.413031755350815
2216	15	-2.3452363255871944
2216	16	-1.8358916292321936
2216	17	-2.0193793676438148
2216	18	-2.2992699274914443
2216	19	-2.5798710672822907
2216	20	-1.9460148392090204
2216	21	-2.4944869955845874
2216	22	-1.9901294049977167
2216	23	-2.953081277683495
2216	24	-2.4122468425529817
2216	25	-2.5988979878352723
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	68718.0
3	0.0
4	1205.0
5	9901.0
6	11980.0
7	9269.0
8	7327.0
9	6228.0
10	5362.0
11	4791.0
12	4233.0
13	3962.0
14	3943.0
15	3942.0
16	3935.0
17	4339.0
18	4653.0
19	5512.0
20	7174.0
21	9460.0
22	11944.0
23	14442.0
24	16688.0
25	18940.0
26	20995.0
27	23161.0
28	26148.0
29	31054.0
30	39308.0
31	49808.0
32	62857.0
33	80997.0
34	105448.0
35	138375.0
36	190067.0
37	296073.0
38	926986.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.19950605888234	18.144905856223716	12.547415339291575	27.108172745602367
2	14.440280757182736	19.50490517264592	38.82814393293422	27.226670137237125
3	18.48667972887908	23.672874582570785	28.52288272588961	29.317562962660524
4	12.24911489259381	15.300649407745162	35.43812143691557	37.012114262745456
5	13.724758244021325	38.02252964267271	33.81061622031975	14.442095892986217
6	34.14144025623866	34.71847994746677	16.91606936146357	14.224010434831005
7	30.024817237744468	30.94361171617843	19.869458097117647	19.162112948959454
8	27.284457783361095	35.21182262472358	19.23568725716058	18.268032334754743
9	27.57416664460628	14.368894227253087	18.703737687500197	39.35320144064043
10	16.61115716332899	28.099313016600792	31.886047015748957	23.40348280432126
11	36.2777358328051	21.824844074610912	22.48349229805066	19.413927794533336
12	24.308109701200287	23.990375104464835	29.669942701905917	22.031572492428964
13	29.929347945554536	20.527721364698557	25.08903845644843	24.453892233298475
14	22.21428215759817	20.80965332421796	26.158161601398888	30.817902916784984
15	24.685851189835255	28.934532141703457	22.3997702882165	23.979846380244787
16	23.87157817099881	26.889032650545225	25.480956017008538	23.75843316144743
17	22.861890434151434	27.22273986847648	26.19954986458703	23.71581983278506
18	22.805873971776798	26.15199579379549	27.976598501270473	23.065531733157236
19	24.45659439202362	25.709116617665135	26.514389583264176	23.31989940704707
20	24.844203360147283	25.45859748033813	26.855114956656863	22.84208420285772
21	24.56164039439804	25.223665676783806	26.230161222310944	23.98453270650721
22	24.558230337481877	26.09760561560656	26.261160874495786	23.083003172415772
23	23.776020814408593	25.74724399735334	26.756787672845945	23.71994751539212
24	24.169206787112113	25.446870549181888	26.98274063856273	23.401182025143267
25	23.732956520763942	25.657481860287767	27.089728493086163	23.519833125862128
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	73.0
1	73.0
2	227.5
3	382.0
4	382.0
5	382.0
6	1096.5
7	1811.0
8	1811.0
9	1811.0
10	2286.5
11	2762.0
12	2762.0
13	2762.0
14	3967.0
15	5172.0
16	5172.0
17	5172.0
18	10344.0
19	15516.0
20	15516.0
21	15516.0
22	26094.5
23	36673.0
24	36673.0
25	36673.0
26	56344.5
27	76016.0
28	76016.0
29	76016.0
30	99639.5
31	123263.0
32	123263.0
33	123263.0
34	143801.0
35	164339.0
36	164339.0
37	164339.0
38	184145.0
39	203951.0
40	203951.0
41	203951.0
42	226387.5
43	248824.0
44	248824.0
45	248824.0
46	274319.0
47	299814.0
48	299814.0
49	299814.0
50	299790.5
51	299767.0
52	299767.0
53	299767.0
54	277850.5
55	255934.0
56	255934.0
57	255934.0
58	229549.0
59	203164.0
60	203164.0
61	203164.0
62	172826.0
63	142488.0
64	142488.0
65	142488.0
66	112032.5
67	81577.0
68	81577.0
69	81577.0
70	61020.5
71	40464.0
72	40464.0
73	40464.0
74	28958.0
75	17452.0
76	17452.0
77	17452.0
78	12119.0
79	6786.0
80	6786.0
81	6786.0
82	4521.5
83	2257.0
84	2257.0
85	2257.0
86	1439.0
87	621.0
88	621.0
89	621.0
90	365.0
91	109.0
92	109.0
93	109.0
94	58.5
95	8.0
96	8.0
97	8.0
98	5.0
99	2.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08227074431697114
2	0.043288586840718186
3	0.018122890242124507
4	0.005248460787941998
5	0.019199497583240813
6	0.05840594825555967
7	0.07809889087014545
8	0.05194630420886182
9	0.022160167771310656
10	0.04835761307180746
11	0.005113884870302459
12	2.691518352790768E-4
13	0.00592134037613969
14	0.0010766073411163071
15	0.015476230528546915
16	0.01018291110139174
17	0.005293319427155177
18	0.006145633572205587
19	0.0019289214861667172
20	0.0016597696508876404
21	2.2429319606589735E-4
22	4.037277529186152E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2229225.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.78434373458012
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.32822273763644	33.60587969537333
2	15.018093355469395	14.352594702715269
3	5.806389366932127	8.323635160988875
4	2.8739058398742396	5.493108180534715
5	1.640391486188287	3.9192515317649925
6	1.0226597152822048	2.9320274015131624
7	0.6936414343829317	2.3201640510370845
8	0.4725899229887249	1.8065919460473583
9	0.35269477567759694	1.516795955493203
>10	1.6352557143451927	13.582163555966789
>50	0.09472434372715638	3.1255993946469953
>100	0.053992031176445715	4.8113592361533595
>500	0.004708402733818967	1.592194616098419
>1k	0.00254253747626217	2.0405055747290732
>5k	1.8833610935275333E-4	0.5781289969373747
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	7700	0.3454115219414819	No Hit
TATCAACGCAGAGTACTTTTTTTTT	5148	0.2309322746694479	No Hit
GGTATCAACGCAGAGTACTTTTTTT	4625	0.20747120636095506	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2907	0.13040406419271272	No Hit
GTCCTACAGTGGACATTTCTAAATT	2903	0.13022462963585998	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2886	0.12946203276923593	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2758	0.12372012694994898	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	4.485863921317947E-5	0.0	0.0	0.0	0.0
9	4.485863921317947E-5	0.0	0.0	0.0	0.0
10	4.485863921317947E-5	0.0	0.0	0.0	0.0
11	4.485863921317947E-5	0.0	0.0	0.0	0.0
12	4.485863921317947E-5	0.0	0.0	0.0	0.0
13	4.485863921317947E-5	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1905	0.0	12.982894	1
CCAACGA	205	0.0	12.500792	19
CCCCGTA	305	0.0	12.449059	15
CTAATAC	160	3.6379788E-12	11.889512	3
TCCAACG	255	0.0	11.538753	18
CCCGTAC	335	0.0	11.334217	16
CCGTACA	340	0.0	11.166785	17
TAGGACC	555	0.0	11.139724	4
TACCGTG	105	3.4405093E-6	10.864306	7
GCGCCGA	70	0.0015012653	10.84895	11
GTCCTAA	625	0.0	10.806162	1
ATTTAGA	370	0.0	10.797935	1
ATACCGT	80	3.7304964E-4	10.700321	6
TAATACT	235	0.0	10.523483	4
TTAGGAC	940	0.0	10.422297	3
TTACCCT	185	5.820766E-11	10.282822	4
GTAGGAC	1400	0.0	10.258951	3
TTTAGAA	455	0.0	10.243274	2
TATACAC	660	0.0	10.232187	3
CGGTTTC	130	3.885525E-7	10.222591	13
>>END_MODULE