FastQCFastQC Report
Thu 26 May 2016
SRR1512876_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512876_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342056
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT67090.2864577106610602No Hit
GTCCTACAGTGGACATTTCTAAATT40180.1715586646946102No Hit
TATCAACGCAGAGTACTTTTTTTTT38810.16570910345440074No Hit
GGTATCAACGCAGAGTACTTTTTTT37000.15798085101295614No Hit
GTCCTAAAGTGTGTATTTCTCATTT34500.1473064691877564No Hit
GTACTGGTTCACTATCGGTCAGTCA33180.14167039558405095No Hit
CTGTAGGACGTGGAATATGGCAAGA32050.13684557499906064No Hit
CTTTAGGACGTGAAATATGGCGAGG29710.12685435361067368No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20550.012.1862951
TAGGACC6100.011.9900244
TGCACCG1052.7271926E-711.7601595
GTACGCT907.477309E-611.6093873
CTTAGAC1604.5474735E-1111.2795753
CGGACTG951.3631399E-510.9983665
GTCCTAC18250.010.6959341
AGACCCG1252.2460699E-710.638425
GTATCAA44400.010.6371781
GCACCGT1355.7018042E-810.5542146
GTGTAGG3450.010.487991
TGTACGC1106.0590046E-610.3620982
GGCGAGG8050.010.26577519
TCCTACA20000.010.2584772
GTGCTAG1951.4551915E-1110.2544521
CTACGAT750.002651691210.1318294
TGTAGGA18700.010.1081252
GTAGAAC5750.010.0767653
CAACCCG856.6046073E-410.057339
GTCCTAA10600.09.9711411
Produced by FastQC (version 0.11.5)