##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512876_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2342056
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.893676325416642	31.0	30.0	33.0	23.0	34.0
2	29.05682998186209	31.0	30.0	34.0	22.0	34.0
3	29.270516588843307	31.0	30.0	34.0	23.0	34.0
4	32.700505026352914	35.0	33.0	37.0	25.0	37.0
5	32.93464374891121	35.0	35.0	37.0	26.0	37.0
6	32.82882006237255	37.0	35.0	37.0	25.0	37.0
7	32.74547235420502	37.0	33.0	37.0	25.0	37.0
8	32.71414987515243	37.0	33.0	37.0	25.0	37.0
9	34.03795340504241	39.0	34.0	39.0	25.0	39.0
10	33.88801463329656	38.0	34.0	39.0	23.0	39.0
11	33.9827941774236	39.0	34.0	39.0	25.0	39.0
12	33.877359465358644	39.0	34.0	39.0	23.0	39.0
13	33.861540031493696	39.0	34.0	39.0	23.0	39.0
14	34.73121778471565	39.0	34.0	41.0	19.0	41.0
15	34.90419998497047	39.0	34.0	41.0	23.0	41.0
16	34.85688728194373	39.0	34.0	41.0	22.0	41.0
17	34.61495583367776	39.0	34.0	41.0	19.0	41.0
18	34.61387772111342	39.0	34.0	41.0	19.0	41.0
19	34.73008928906909	39.0	34.0	41.0	19.0	41.0
20	34.6820101654273	39.0	34.0	41.0	19.0	41.0
21	34.58636813124878	39.0	34.0	41.0	18.0	41.0
22	34.609622485542616	39.0	34.0	41.0	18.0	41.0
23	34.274793600153025	39.0	34.0	41.0	16.0	41.0
24	34.33941886957442	39.0	34.0	41.0	16.0	41.0
25	34.052933832495896	39.0	34.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5112814457913508
1101	2	0.10781096424346259
1101	3	0.48623579898058367
1101	4	0.6480043198629346
1101	5	0.5040480693032592
1101	6	0.5876656311069652
1101	7	0.3952825315585571
1101	8	0.6260372195759771
1101	9	0.722167845659115
1101	10	0.7429966144108775
1101	11	0.6838065896109313
1101	12	0.46928980621120076
1101	13	0.9272938748566233
1101	14	0.9632243792096276
1101	15	0.769528109077946
1101	16	0.6775335811541794
1101	17	0.10993597257439092
1101	18	0.4708950430502554
1101	19	-0.05516242698688245
1101	20	-0.5914225856819897
1101	21	0.4688159829638323
1101	22	0.5394668899805097
1101	23	0.047702155673498225
1101	24	0.5868641477519603
1101	25	0.29733860534240364
1102	1	0.9786774875220168
1102	2	0.9775466290044221
1102	3	0.9526758667167421
1102	4	1.1471054014685222
1102	5	1.0720524127095956
1102	6	0.9237431748969414
1102	7	0.9020630159520167
1102	8	0.9784189279408437
1102	9	1.21693597981767
1102	10	1.229838625190716
1102	11	1.0853070035957515
1102	12	1.1249946169271041
1102	13	1.045492784883919
1102	14	1.2314484530200644
1102	15	1.2487240450333061
1102	16	1.2028452717607792
1102	17	1.350623967888808
1102	18	1.3885787199347916
1102	19	1.1501216708758832
1102	20	1.3598609858018875
1102	21	1.5052368906333555
1102	22	1.2950918228562784
1102	23	1.2215224706516281
1102	24	0.7715706087874352
1102	25	1.1273382057190489
1103	1	0.32063689349199365
1103	2	0.5025241057315135
1103	3	0.6852123898610891
1103	4	0.8494129639984465
1103	5	0.6580881277027473
1103	6	0.2203929125219659
1103	7	0.19993463781208476
1103	8	0.3272000149336165
1103	9	0.41813128617327067
1103	10	0.7520648891597617
1103	11	0.6090108367680287
1103	12	0.6342692874718239
1103	13	0.7805636173612385
1103	14	0.7470827653811867
1103	15	0.7526176436576932
1103	16	0.7064698281731623
1103	17	0.28819205852620655
1103	18	0.6743272769540241
1103	19	0.5583678412364463
1103	20	0.7985744980428535
1103	21	0.7002690432647611
1103	22	0.6830821124436
1103	23	0.07563913145299495
1103	24	0.3202183358436983
1103	25	0.6293728706506911
1104	1	0.8857569486915082
1104	2	0.8994665148383767
1104	3	0.984948149526133
1104	4	1.171673158927625
1104	5	0.9369314966467996
1104	6	0.8960465593760887
1104	7	1.0659473543184603
1104	8	0.8161916921387942
1104	9	1.2030044185333608
1104	10	1.1443088099666312
1104	11	0.9482182363503426
1104	12	1.0819958845699773
1104	13	1.23434699969782
1104	14	1.3798620079745731
1104	15	1.2345226916944085
1104	16	1.1908277223945305
1104	17	1.4741370491545993
1104	18	1.2999602585477845
1104	19	0.9336033079261128
1104	20	1.0951491088973597
1104	21	1.2140766798008826
1104	22	1.076556684246981
1104	23	1.3895860167706928
1104	24	1.2266873160048277
1104	25	0.6774683321683455
1105	1	0.7010777559493171
1105	2	0.7276149323013286
1105	3	0.9043053541501642
1105	4	1.075015585969055
1105	5	0.7948408451390563
1105	6	0.9810054535187831
1105	7	0.8845384586337914
1105	8	0.9095534007881128
1105	9	0.6324418010476833
1105	10	0.8677003969107773
1105	11	0.8707454190695927
1105	12	1.0335873990446913
1105	13	0.9583254778497761
1105	14	1.0861111137527217
1105	15	1.0128671139203789
1105	16	1.008876208737398
1105	17	1.3651022540750333
1105	18	1.212234106636565
1105	19	0.8770714420663595
1105	20	0.9973365317306673
1105	21	1.0533662077651371
1105	22	0.9681502368794384
1105	23	1.1994143427866675
1105	24	1.2049943695979053
1105	25	1.41664256916102
1106	1	0.7638982712499001
1106	2	0.818624797584274
1106	3	0.7682041951576117
1106	4	0.8374744173815927
1106	5	0.6519681947413645
1106	6	0.8090841823366688
1106	7	0.7507906406637872
1106	8	0.732982588436812
1106	9	0.9936422981137838
1106	10	0.833847478418221
1106	11	0.44757951098277715
1106	12	0.8161273050271163
1106	13	0.8021305959163669
1106	14	1.1122157889924082
1106	15	0.8181043728070847
1106	16	0.9566780929875378
1106	17	1.1536096614818945
1106	18	0.9790113151053816
1106	19	1.0664630896476268
1106	20	1.1674109919465536
1106	21	1.2318987080519577
1106	22	1.047307239322663
1106	23	1.3309196045833929
1106	24	0.9395614597568311
1106	25	1.2357001821931846
1107	1	0.8302972331902758
1107	2	0.8991263925319402
1107	3	0.7806234363511457
1107	4	0.8917730686010046
1107	5	0.9269847466422831
1107	6	0.9724837803661472
1107	7	0.6345500808581193
1107	8	0.8893437157733501
1107	9	0.8535553364049306
1107	10	0.999742855048531
1107	11	0.8806512130497381
1107	12	1.01534560053274
1107	13	0.7933185174287587
1107	14	1.1092169566323022
1107	15	0.8294684856872152
1107	16	0.9715068834319069
1107	17	1.2611908159983898
1107	18	1.300916700246404
1107	19	1.0669444384164066
1107	20	1.2204909153323698
1107	21	1.0449902428694173
1107	22	1.105177911834808
1107	23	1.3911342684159038
1107	24	1.1729187522497355
1107	25	1.3998052662402287
1108	1	0.7666095350746609
1108	2	0.7312472336685367
1108	3	0.6774059136581592
1108	4	0.9177829604438159
1108	5	0.6683160152876226
1108	6	0.7149496982231227
1108	7	0.7228723815364617
1108	8	0.6853179155171247
1108	9	0.870431714538654
1108	10	0.8151173091994863
1108	11	0.5971735146383068
1108	12	0.7166509733484858
1108	13	0.7430221831222426
1108	14	0.7746066570098193
1108	15	0.8265253381833872
1108	16	0.6539575664401056
1108	17	1.0334062178729937
1108	18	1.0443201472589436
1108	19	0.9541269129927556
1108	20	1.0766379321919644
1108	21	1.0029749420295104
1108	22	1.017237904598538
1108	23	1.3131071528881932
1108	24	1.2573780118292603
1108	25	1.3430679701016146
1109	1	0.9472067475824169
1109	2	0.7571754051339141
1109	3	0.9209535787142471
1109	4	0.9946380614281694
1109	5	0.863030065596611
1109	6	0.9738891014001325
1109	7	0.8615273705198376
1109	8	0.7976943065676565
1109	9	0.9222133289899404
1109	10	1.0204116138026436
1109	11	0.8779611542232004
1109	12	0.8507066678703197
1109	13	1.017395592936893
1109	14	1.1250842136270478
1109	15	0.9615681683985855
1109	16	0.6156751331509724
1109	17	1.027289137892211
1109	18	1.0416661219130745
1109	19	0.9526043996093705
1109	20	0.9273776382909915
1109	21	0.8913894450279614
1109	22	0.8514331205080978
1109	23	1.1697962762038756
1109	24	0.9226436077123239
1109	25	0.558233137682528
1110	1	0.5396116628008123
1110	2	0.6483391103140583
1110	3	0.5047779260132756
1110	4	0.508391239801739
1110	5	0.47129125588606513
1110	6	0.48282080987063836
1110	7	0.37290166012108017
1110	8	0.5073778762704109
1110	9	0.720285656866551
1110	10	0.5639053769469982
1110	11	0.6119558473592477
1110	12	0.6335484044320623
1110	13	0.6410382796387637
1110	14	0.7002066444937824
1110	15	0.6012908860058204
1110	16	0.7412747987869039
1110	17	0.6335669148374095
1110	18	0.1796497883616084
1110	19	0.5195248782428763
1110	20	0.6385939874949642
1110	21	0.7992082856438714
1110	22	0.7560779585963004
1110	23	0.8337753751193588
1110	24	0.7169830638378585
1110	25	0.9697917188332497
1111	1	0.5791914328662173
1111	2	0.5545460424694397
1111	3	0.5278976428372886
1111	4	0.5433342724064829
1111	5	0.539558419053435
1111	6	0.4432556295636658
1111	7	0.3788873890927462
1111	8	0.5650702702926509
1111	9	0.6439834648114555
1111	10	0.6689465874031413
1111	11	0.3862253493771206
1111	12	0.032504787788070644
1111	13	0.44699505355137603
1111	14	0.5086745402914801
1111	15	0.44192246542572633
1111	16	0.45152163505390064
1111	17	0.02723563304856924
1111	18	0.4334784776625966
1111	19	0.5002619079476247
1111	20	0.5851079330594544
1111	21	0.459316855892439
1111	22	0.1851706102961188
1111	23	0.6610146785818429
1111	24	0.6566785419105798
1111	25	0.7685512526792451
1112	1	0.18358052071473452
1112	2	0.1421033712913946
1112	3	0.20850927608831427
1112	4	0.14540520832045445
1112	5	0.08754583304963859
1112	6	-0.032593662322220496
1112	7	-0.11430420592873247
1112	8	-0.05256331762262789
1112	9	-0.06929594943036932
1112	10	0.08308292704553821
1112	11	-0.03169035464982528
1112	12	-0.0801699732209542
1112	13	-0.06170173085168784
1112	14	-0.011492444954704695
1112	15	-0.29347695893402914
1112	16	-0.1207442991333707
1112	17	0.1450809735343057
1112	18	-0.1617749691087056
1112	19	-0.02398175440427508
1112	20	-0.19769040638350788
1112	21	-0.07129459426690943
1112	22	-0.1705651872822287
1112	23	0.015564675849553566
1112	24	0.1579378605729147
1112	25	0.14148168291089291
1113	1	-0.08532775619961441
1113	2	0.08701196402373057
1113	3	0.023827871434797032
1113	4	0.12070591196405189
1113	5	-0.05105090559953851
1113	6	-0.3951215370261707
1113	7	-0.3123450323786585
1113	8	-0.2458707103581972
1113	9	-0.2413754630260314
1113	10	-0.2789661290855321
1113	11	-0.3016525904068388
1113	12	-0.4465349528001923
1113	13	-0.17016798254691423
1113	14	0.10000511540712154
1113	15	-0.2519886024904636
1113	16	-0.3020005991024277
1113	17	-0.22790962753261113
1113	18	-0.3405206276575683
1113	19	-0.6458732000611249
1113	20	-0.4434588080124868
1113	21	-0.2927214359503054
1113	22	-0.7629836583939493
1113	23	-4.43244643804217
1113	24	-1.8985693570678066
1113	25	-0.9732779815520232
1114	1	-0.6122310056337241
1114	2	-0.9217326657249245
1114	3	-0.7967054987665385
1114	4	-0.6457611428952461
1114	5	-0.6250203541826451
1114	6	-1.261683605438872
1114	7	-1.0562331311221556
1114	8	-0.9179942338608136
1114	9	-1.1593284651454496
1114	10	-0.7437209544062071
1114	11	-0.9590165820350549
1114	12	-1.8253325901102215
1114	13	-1.1620355724825373
1114	14	-2.9527475566652193
1114	15	-1.704296799395017
1114	16	-1.8073668792924025
1114	17	-1.6529813657070278
1114	18	-1.5473296136452745
1114	19	-1.7034672245028446
1114	20	-1.1762979777175744
1114	21	-0.9989957868503652
1114	22	-1.1598720380598735
1114	23	-5.290533106287494
1114	24	-2.6131029743632155
1114	25	-2.487531632440458
1115	1	-1.3486470738299658
1115	2	-1.2884208077828028
1115	3	-1.1393725954307783
1115	4	-1.0954822811448501
1115	5	-1.0122831453860073
1115	6	-1.1090509477600357
1115	7	-1.5056255269236551
1115	8	-1.8438484499918957
1115	9	-3.1651837538217222
1115	10	-3.919485253049803
1115	11	-2.599819727384027
1115	12	-2.9327252915136413
1115	13	-2.3057235705548926
1115	14	-2.9059326485808725
1115	15	-2.6401551440904782
1115	16	-2.611945251492024
1115	17	-2.4402219713865207
1115	18	-5.507942374876144
1115	19	-3.2061062733756422
1115	20	-3.4011072703666727
1115	21	-3.01101872462511
1115	22	-2.5742861639208456
1115	23	-3.2016369609855353
1115	24	-3.027230084191846
1115	25	-3.1102975795713235
1116	1	-1.0771338434331383
1116	2	-0.9017347292353683
1116	3	-1.343346978004238
1116	4	-1.1570017616018333
1116	5	-1.2410541453475084
1116	6	-3.345620988135785
1116	7	-1.961920071385105
1116	8	-2.3254429552325604
1116	9	-3.2174525136269168
1116	10	-2.455992461973853
1116	11	-2.0029744738204727
1116	12	-2.0016400248123247
1116	13	-3.130242153505641
1116	14	-6.203001045533682
1116	15	-4.119449188065875
1116	16	-3.0356922618097393
1116	17	-3.9977241790593574
1116	18	-2.6031463109101765
1116	19	-2.414327117657109
1116	20	-3.1448963106846755
1116	21	-4.009740139171928
1116	22	-3.593366592991387
1116	23	-2.6012529642675695
1116	24	-3.554182190323761
1116	25	-3.500783622507253
1201	1	0.7988976910688841
1201	2	0.9051458855703167
1201	3	1.250605583511657
1201	4	1.220503230063457
1201	5	0.7882262567072473
1201	6	1.1399617348143565
1201	7	1.2089787162224823
1201	8	1.2136039049249234
1201	9	1.7028952608623342
1201	10	1.497536148184281
1201	11	1.6663581215576286
1201	12	1.4924350712919505
1201	13	1.6093085508229095
1201	14	1.616011671109824
1201	15	1.7398790764047192
1201	16	1.6111116004176793
1201	17	1.8806317835927828
1201	18	1.7426702745029061
1201	19	1.871409738496574
1201	20	1.7636392734121316
1201	21	1.9125758430543414
1201	22	1.7015525067032229
1201	23	2.123646908029535
1201	24	1.9567641456761535
1201	25	2.0157629628796627
1202	1	0.7887321321065777
1202	2	0.7804958681285648
1202	3	0.8981306904571476
1202	4	1.0394784811329103
1202	5	0.8331364775749321
1202	6	1.1432779629892167
1202	7	1.1008168934674174
1202	8	1.1891533201671791
1202	9	1.4657551117812417
1202	10	1.2126996875751104
1202	11	1.278513187928091
1202	12	1.5001438674437466
1202	13	1.4172920545958405
1202	14	1.8255754895687062
1202	15	1.513019234665684
1202	16	1.2952219332797839
1202	17	1.5589702729533101
1202	18	1.571944192655181
1202	19	1.573644577940641
1202	20	1.3734729800601926
1202	21	1.5741656589061392
1202	22	1.63666000298538
1202	23	1.7771125385948352
1202	24	1.7037577381266331
1202	25	1.9760382212930168
1203	1	0.9169322118230525
1203	2	1.051331718269907
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2211	15	-0.25378451806532354
2211	16	-0.2950820481497374
2211	17	0.11529668040704877
2211	18	-0.3104227930186312
2211	19	-0.16122400223674305
2211	20	-0.21795509645362898
2211	21	-0.14411369811977437
2211	22	-0.1537269964023622
2211	23	0.12750635503414998
2211	24	-0.3080124522000247
2211	25	0.20231124979611081
2212	1	0.22777486228476462
2212	2	0.26776109294359074
2212	3	-0.13219306944828446
2212	4	0.2799856830942957
2212	5	0.02236308761827388
2212	6	-8.03951112615664E-4
2212	7	0.10476921604524136
2212	8	0.25654966883842434
2212	9	0.2715101137513827
2212	10	-0.06363453597027302
2212	11	-0.16566066610631935
2212	12	0.131543543464943
2212	13	0.26673056429331155
2212	14	0.2711295371147884
2212	15	-0.024061015761176918
2212	16	-0.07217003729944338
2212	17	0.19044656558498474
2212	18	0.317470877126091
2212	19	0.12020449213003559
2212	20	0.285293828534428
2212	21	0.3038688242666794
2212	22	0.45234123410485694
2212	23	0.41543616715750886
2212	24	0.369605336743156
2212	25	0.50494676295056
2213	1	-0.3252251797841872
2213	2	-0.12156563856936131
2213	3	-0.33553972962431544
2213	4	-0.09705721521603294
2213	5	-0.19929483471643294
2213	6	-0.07500208685710419
2213	7	-0.3111061787267886
2213	8	-0.11011369241065694
2213	9	-0.7352562262799296
2213	10	-0.18004458203576945
2213	11	-0.28093222118509686
2213	12	-0.30452697611665513
2213	13	-0.4607983806474465
2213	14	-0.4429662659063638
2213	15	-0.46634196423573115
2213	16	-0.41723399712959974
2213	17	-0.3844236967750234
2213	18	-0.3667566467856034
2213	19	-0.38209074476323224
2213	20	-0.22604916262808672
2213	21	-0.5029747423389921
2213	22	-0.7137629857114263
2213	23	-0.438305565542386
2213	24	-0.1347168723575649
2213	25	-0.045715071796593065
2214	1	-2.8805276473386847
2214	2	-1.5367189182389644
2214	3	-1.6572758633666957
2214	4	-3.533704882699986
2214	5	-1.883276038422025
2214	6	-1.5833726055501565
2214	7	-1.4152040103369892
2214	8	-1.2968233769025233
2214	9	-1.216248214026514
2214	10	-1.148197233546668
2214	11	-1.1665235507754659
2214	12	-1.0382549554920715
2214	13	-1.5103070262961396
2214	14	-1.2648890431308004
2214	15	-1.7016819271394255
2214	16	-1.594812561116136
2214	17	-3.620848890426714
2214	18	-3.6534238348155874
2214	19	-2.3896657257147425
2214	20	-2.2254887352814023
2214	21	-2.17812403610192
2214	22	-3.0697867846474303
2214	23	-1.9499635016668222
2214	24	-3.0806027232985684
2214	25	-3.0767676087833813
2215	1	-1.8126457947021564
2215	2	-3.2942823187986185
2215	3	-1.930250868409697
2215	4	-3.8402656519160736
2215	5	-2.2107779012456064
2215	6	-1.9132938373976849
2215	7	-2.0673915883444813
2215	8	-1.496964019615021
2215	9	-3.9801904614431685
2215	10	-2.249768816682863
2215	11	-1.787427219499385
2215	12	-1.7115533863789807
2215	13	-1.7304261946104376
2215	14	-1.4518973690446444
2215	15	-1.6436536721008252
2215	16	-1.8174632982553547
2215	17	-2.0713780221432216
2215	18	-1.5959538841902372
2215	19	-1.8102451452473076
2215	20	-1.5912511793358846
2215	21	-2.286847888739196
2215	22	-2.212736292117249
2215	23	-2.1965598020673767
2215	24	-2.015607676481906
2215	25	-2.898961912042452
2216	1	-2.79727177004969
2216	2	-2.9993053277206165
2216	3	-4.329584699957998
2216	4	-4.808997934763859
2216	5	-4.663563071093861
2216	6	-3.6328352961513417
2216	7	-5.129437628770461
2216	8	-2.973810008384717
2216	9	-3.857651315225546
2216	10	-2.709461955782931
2216	11	-2.9747772874923157
2216	12	-3.3192816461056367
2216	13	-2.917326246055012
2216	14	-3.71597155367634
2216	15	-3.3186577474427814
2216	16	-3.5426254121828613
2216	17	-3.1852904280486882
2216	18	-2.8672773082678518
2216	19	-3.5012818628434026
2216	20	-3.990096463830035
2216	21	-3.7677077541364703
2216	22	-3.560066008099433
2216	23	-3.4941356263626275
2216	24	-3.9468102587314817
2216	25	-3.9936056071885275
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107586.0
3	1.0
4	965.0
5	6010.0
6	7129.0
7	6509.0
8	5657.0
9	5263.0
10	4747.0
11	4412.0
12	4308.0
13	4215.0
14	4144.0
15	4397.0
16	4409.0
17	4822.0
18	5575.0
19	6787.0
20	8512.0
21	11140.0
22	13739.0
23	16673.0
24	19617.0
25	22309.0
26	25041.0
27	27649.0
28	31364.0
29	36902.0
30	44973.0
31	55554.0
32	69879.0
33	90120.0
34	115659.0
35	153024.0
36	210188.0
37	328670.0
38	874107.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.3608265090242	17.933573201694216	12.239798668242177	24.46580162103941
2	15.47382512506165	19.72355130249867	41.09665669589575	23.705966876543922
3	19.726952896983473	25.227009048763232	28.27397969227117	26.772058361982126
4	12.666151969910736	15.825089075561355	37.251263113664855	34.25749584086305
5	13.681886175445609	38.44846500358485	34.0748578975889	13.794790923380635
6	32.47718692513295	35.38273192847666	18.323359178537014	13.816721967853383
7	29.540154983773064	31.296509553486924	20.953361720674547	18.209973742065465
8	26.546961042602334	35.37289651081086	20.280516514195934	17.79962593239087
9	28.339726135106908	14.494225112087694	19.409845256592362	37.75620349621304
10	16.88360639904546	27.919455515317694	32.59493481551504	22.602003270121802
11	35.18945300564639	22.1958318501213	23.782145816444658	18.832569327787652
12	24.622578450344985	24.26874841868448	30.270909264970356	20.837763866000177
13	29.708056302542197	21.46702145979968	25.353365157599345	23.471557080058783
14	22.127879428114294	21.729981607214896	26.865773693531075	29.27636527113974
15	24.326156063003634	29.813697508545506	22.859863109525627	23.000283318925234
16	23.073011282174882	27.346186833431407	26.700734125534225	22.880067758859486
17	22.49837165291333	27.830204000690657	26.734082800380886	22.937341546015123
18	22.255758898367205	26.607719618570886	28.93811970370902	22.198401779352892
19	24.314281074243123	26.159931278527104	26.991854283125633	22.533933364104143
20	24.89386416493513	25.89774206534737	27.373173339669716	21.835220430047787
21	24.590963223973407	25.72275021761026	26.274414118835498	23.41187243958084
22	24.594416934006578	26.827891224497996	26.379632075318476	22.19805976617695
23	23.596039113116912	26.63192129510915	26.95518573602065	22.816853855753287
24	24.024049819314385	25.949325975992917	27.452076431553145	22.574547773139553
25	23.592161273628506	26.3739288032639	27.287381363345574	22.74652855976202
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	302.0
1	302.0
2	675.0
3	1048.0
4	1048.0
5	1048.0
6	1824.0
7	2600.0
8	2600.0
9	2600.0
10	3003.0
11	3406.0
12	3406.0
13	3406.0
14	4887.5
15	6369.0
16	6369.0
17	6369.0
18	12919.5
19	19470.0
20	19470.0
21	19470.0
22	32210.5
23	44951.0
24	44951.0
25	44951.0
26	68563.5
27	92176.0
28	92176.0
29	92176.0
30	121054.0
31	149932.0
32	149932.0
33	149932.0
34	172173.0
35	194414.0
36	194414.0
37	194414.0
38	214427.0
39	234440.0
40	234440.0
41	234440.0
42	253772.0
43	273104.0
44	273104.0
45	273104.0
46	295559.0
47	318014.0
48	318014.0
49	318014.0
50	313835.0
51	309656.0
52	309656.0
53	309656.0
54	278505.5
55	247355.0
56	247355.0
57	247355.0
58	216205.5
59	185056.0
60	185056.0
61	185056.0
62	156413.0
63	127770.0
64	127770.0
65	127770.0
66	99603.5
67	71437.0
68	71437.0
69	71437.0
70	53978.5
71	36520.0
72	36520.0
73	36520.0
74	25995.5
75	15471.0
76	15471.0
77	15471.0
78	10665.0
79	5859.0
80	5859.0
81	5859.0
82	3924.0
83	1989.0
84	1989.0
85	1989.0
86	1267.5
87	546.0
88	546.0
89	546.0
90	337.0
91	128.0
92	128.0
93	128.0
94	80.5
95	33.0
96	33.0
97	33.0
98	21.5
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.34149482335179004
2	0.00900917826046858
3	0.008069832659851003
4	0.009564246115378967
5	0.01105865957090693
6	0.07860614776077088
7	0.07732522194174692
8	0.10127853475749513
9	0.0821500425267372
10	0.08889625184026342
11	0.08441301147367954
12	0.09530088093538328
13	0.0816803697264284
14	0.0857366348200043
15	0.08270511038164757
16	0.08509617191049232
17	0.09649641169980565
18	0.09944254108356076
19	0.09290981940653853
20	0.09094573315070179
21	0.07954549336138846
22	0.09982681882926797
23	0.09290981940653853
24	0.08881085678566182
25	0.08680407300252428
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2342056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.44159064571292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.93477302502671	35.56569601095642
2	14.412105068891687	14.251147983182937
3	5.550763989048505	8.23315802752502
4	2.7134959592688843	5.3663822574787305
5	1.4998121063651302	3.7076548104194598
6	0.9281888203048794	2.7534679017264616
7	0.6022390252021934	2.084295874844402
8	0.42863250878533776	1.6953818429447691
9	0.3052169672869752	1.358137111925572
>10	1.4772964204213048	12.63929022277311
>50	0.08753426735425553	2.9817029638864465
>100	0.05177038840365924	4.747100924228279
>500	0.005128891114746073	1.710894975461089
>1k	0.002955632167819336	2.617337938569653
>5k	8.693035787703929E-5	0.2883511540776032
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6709	0.2864577106610602	No Hit
GTCCTACAGTGGACATTTCTAAATT	4018	0.1715586646946102	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3881	0.16570910345440074	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3700	0.15798085101295614	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3450	0.1473064691877564	No Hit
GTACTGGTTCACTATCGGTCAGTCA	3318	0.14167039558405095	No Hit
CTGTAGGACGTGGAATATGGCAAGA	3205	0.13684557499906064	No Hit
CTTTAGGACGTGAAATATGGCGAGG	2971	0.12685435361067368	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.269752730079895E-5
2	0.0	0.0	0.0	0.0	4.269752730079895E-5
3	0.0	0.0	0.0	0.0	4.269752730079895E-5
4	0.0	0.0	0.0	0.0	4.269752730079895E-5
5	0.0	0.0	0.0	0.0	4.269752730079895E-5
6	0.0	0.0	0.0	0.0	4.269752730079895E-5
7	0.0	0.0	0.0	0.0	4.269752730079895E-5
8	0.0	0.0	0.0	0.0	4.269752730079895E-5
9	0.0	0.0	0.0	0.0	4.269752730079895E-5
10	0.0	0.0	0.0	0.0	4.269752730079895E-5
11	0.0	0.0	0.0	0.0	4.269752730079895E-5
12	0.0	0.0	0.0	0.0	8.53950546015979E-5
13	0.0	0.0	0.0	0.0	8.53950546015979E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2055	0.0	12.186295	1
TAGGACC	610	0.0	11.990024	4
TGCACCG	105	2.7271926E-7	11.760159	5
GTACGCT	90	7.477309E-6	11.609387	3
CTTAGAC	160	4.5474735E-11	11.279575	3
CGGACTG	95	1.3631399E-5	10.998366	5
GTCCTAC	1825	0.0	10.695934	1
AGACCCG	125	2.2460699E-7	10.63842	5
GTATCAA	4440	0.0	10.637178	1
GCACCGT	135	5.7018042E-8	10.554214	6
GTGTAGG	345	0.0	10.48799	1
TGTACGC	110	6.0590046E-6	10.362098	2
GGCGAGG	805	0.0	10.265775	19
TCCTACA	2000	0.0	10.258477	2
GTGCTAG	195	1.4551915E-11	10.254452	1
CTACGAT	75	0.0026516912	10.131829	4
TGTAGGA	1870	0.0	10.108125	2
GTAGAAC	575	0.0	10.076765	3
CAACCCG	85	6.6046073E-4	10.05733	9
GTCCTAA	1060	0.0	9.971141	1
>>END_MODULE