FastQCFastQC Report
Thu 26 May 2016
SRR1512876_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512876_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2342056
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT85170.3636548400209047No Hit
TATCAACGCAGAGTACTTTTTTTTT56670.24196688721362766No Hit
GGTATCAACGCAGAGTACTTTTTTT47630.20336832253370543No Hit
GTCCTACAGTGGACATTTCTAAATT37560.1603719125418009No Hit
CTGTAGGACGTGGAATATGGCAAGA31660.13518037143432948No Hit
GTCCTAAAGTGTGTATTTCTCATTT31290.13360056292419994No Hit
CTTTAGGACGTGAAATATGGCGAGG30240.12911732255761604No Hit
GTACTGGTTCACTATCGGTCAGTCA30060.12834876706620166No Hit
ACGCAGAGTACTTTTTTTTTTTTTT27320.11664964458578274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTACG456.712627E-414.7926852
TAACCCG500.001487630713.3145555
TAGGACC6450.012.2381854
ACCGATG550.003067201212.0919848
GGTATCA20150.011.9893171
TAGGACA5800.010.9861484
AAGACCG1053.4229015E-610.8690245
CCCGTAC1951.8189894E-1210.709517516
GTATCAA44000.010.700111
GTCCTAC20200.010.6412551
TGTAGGA18950.010.4379592
CTACACG1752.3101165E-1010.3255734
AGGACCT13400.010.2201275
GTAGGAC17800.010.2041033
CCCCGTA2053.6379788E-1210.18753715
CCGTACA2151.8189894E-1210.15436317
TAATACT2350.010.1174424
TCCTACA22050.010.0918022
CGTGCGC1606.204573E-910.09098110
GCACTGT4750.09.8107256
Produced by FastQC (version 0.11.5)