FastQCFastQC Report
Thu 26 May 2016
SRR1512875_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512875_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652216
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55920.21084255580993402No Hit
GTCCTACAGTGGACATTTCTAAATT49390.1862216350402833No Hit
GTCCTAAAGTGTGTATTTCTCATTT44530.1678973356619521No Hit
CTGTAGGACGTGGAATATGGCAAGA42970.16201546178742607No Hit
CTTTAGGACGTGAAATATGGCGAGG35970.1356224379914758No Hit
GTCCTACAGTGTGCATTTCTCATTT34680.13075858074907926No Hit
TATCAACGCAGAGTACTTTTTTTTT32240.12155872674020517No Hit
GGTATCAACGCAGAGTACTTTTTTT31010.11692109541605962No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10050.012.9485384
CATACGG604.0982827E-412.6649922
GGTATCA16300.012.5603151
GACCGGG853.952111E-612.2924937
GTATCAA37750.011.3512911
CCTACAC3550.011.2379513
GTCCTAC25300.011.2161911
CAATACG600.005883293711.0818684
AGGACCT17700.010.9477065
TACACCG803.777172E-410.6860895
CGTGCGC1605.456968E-1010.68568510
TGTAGGA27050.010.6048832
TAATACT2600.010.5947534
TGGCGAG23150.010.58647418
TTAGGAC14600.010.4746433
TCCTACA28550.010.3804152
AAGTCGT2200.010.36226757
GGCGAGG11500.010.32511219
GGTATAC1201.4792222E-610.3160111
CTACACT3500.010.31292254
Produced by FastQC (version 0.11.5)