FastQCFastQC Report
Thu 26 May 2016
SRR1512875_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512875_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2652216
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT66930.2523550118089929No Hit
TATCAACGCAGAGTACTTTTTTTTT47600.17947256181246174No Hit
GTCCTACAGTGGACATTTCTAAATT47350.1785299538197492No Hit
CTGTAGGACGTGGAATATGGCAAGA41430.15620899655231701No Hit
GTCCTAAAGTGTGTATTTCTCATTT38830.14640587342810693No Hit
CTTTAGGACGTGAAATATGGCGAGG37960.14312559761346738No Hit
GGTATCAACGCAGAGTACTTTTTTT37510.14142890322658486No Hit
GTCCTACAGTGTGCATTTCTCATTT31890.12023907555040766No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18500.013.6773761
GTCGCGT604.1189237E-412.65745316
TCGCGTC701.0996737E-412.2044817
TAGGACC9350.011.8994714
CGCGTCC907.536215E-611.60135218
TCTACAC4700.011.53486253
TGGACCG951.34623915E-511.0108915
GTAATAC1408.20728E-910.8699463
CTACACT4900.010.6738234
GGCGAGG11600.010.63700219
TGGCGAG24800.010.5254218
GTCCTAA13300.010.5137721
GTATCAA40700.010.3538581
ATAGGAC2950.010.3172373
TCCAACG2151.8189894E-1210.15424918
AGGACGT24350.010.1147285
GGACGTG24200.010.0591576
GTAGGAC24900.010.0459693
TGTAGGA25600.09.9941932
AGGACCT17350.09.9752865
Produced by FastQC (version 0.11.5)