##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512875_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2652216 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.172970074835533 31.0 30.0 34.0 26.0 34.0 2 30.20335900243419 31.0 30.0 34.0 26.0 34.0 3 30.385907105605273 31.0 30.0 34.0 26.0 34.0 4 33.97680505660173 37.0 35.0 37.0 30.0 37.0 5 33.52849994118126 37.0 35.0 37.0 28.0 37.0 6 33.3887104217756 37.0 35.0 37.0 27.0 37.0 7 33.233358067367064 37.0 35.0 37.0 26.0 37.0 8 33.17617154862198 37.0 35.0 37.0 26.0 37.0 9 34.51889250347634 39.0 35.0 39.0 27.0 39.0 10 34.44080610327364 39.0 35.0 39.0 27.0 39.0 11 34.412631550371465 39.0 35.0 39.0 26.0 39.0 12 34.26858596735711 39.0 35.0 39.0 25.0 39.0 13 34.25849629140311 39.0 35.0 39.0 25.0 39.0 14 35.342999589777 40.0 36.0 41.0 25.0 41.0 15 35.3276724067723 40.0 36.0 41.0 25.0 41.0 16 35.32979289771271 40.0 36.0 41.0 25.0 41.0 17 35.30593322715797 40.0 36.0 41.0 25.0 41.0 18 35.224834628853756 40.0 36.0 41.0 25.0 41.0 19 35.23250368748247 40.0 35.0 41.0 25.0 41.0 20 35.19811131521716 40.0 35.0 41.0 25.0 41.0 21 35.1601426882275 40.0 35.0 41.0 25.0 41.0 22 35.12760951596702 40.0 35.0 41.0 24.0 41.0 23 34.96181645838801 40.0 35.0 41.0 23.0 41.0 24 34.91580700817731 40.0 35.0 41.0 23.0 41.0 25 34.856762420557 40.0 35.0 41.0 22.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6114945833579988 1101 2 0.5269246269989232 1101 3 0.5256546742986785 1101 4 0.5155860558054073 1101 5 0.5839190248270612 1101 6 0.6519910839390732 1101 7 0.7353924118951554 1101 8 0.5360599524264629 1101 9 0.8263883516673332 1101 10 0.42709105779646706 1101 11 0.7421848660403612 1101 12 0.8632373362189938 1101 13 0.9761318750069847 1101 14 1.0526279115385222 1101 15 0.7260619984188068 1101 16 0.9674562316974402 1101 17 1.0234886294422267 1101 18 0.9630866992325693 1101 19 0.8197711906191358 1101 20 0.9095417916882411 1101 21 0.9974180405683484 1101 22 0.9321495894868619 1101 23 1.0287047118602715 1101 24 0.9406184200945944 1101 25 0.9697873768822376 1102 1 0.7833988577915072 1102 2 0.9359800948260677 1102 3 0.5772104215600997 1102 4 0.7280159325736619 1102 5 0.8478428532588467 1102 6 0.8728154372149035 1102 7 0.8340034142689987 1102 8 0.7822914057456742 1102 9 0.9718570355786653 1102 10 0.8972087008582008 1102 11 1.0160321811816644 1102 12 0.9988396512642765 1102 13 1.0636399810630053 1102 14 1.034618187718067 1102 15 1.005980423305644 1102 16 1.0081973800319872 1102 17 1.0292799356140563 1102 18 0.5017935167888226 1102 19 0.7762308986583264 1102 20 0.9992108971883411 1102 21 1.0222197407105043 1102 22 0.6998638761859368 1102 23 0.9426974263808532 1102 24 0.8429238882910326 1102 25 0.9012806785841079 1103 1 0.6802373792737804 1103 2 0.8584251011175255 1103 3 0.6002534095585794 1103 4 0.44324494666619074 1103 5 0.666413460841099 1103 6 0.6774828444473115 1103 7 0.7784584701381476 1103 8 0.828931130563511 1103 9 0.9150713925011971 1103 10 0.7939726631195114 1103 11 0.9953130951939357 1103 12 1.0591672762096138 1103 13 1.00884398011803 1103 14 1.0993029194975534 1103 15 1.0817200256452963 1103 16 1.131323137143177 1103 17 0.941502788936802 1103 18 0.9337670239708018 1103 19 1.0719746562084964 1103 20 0.9091777057688546 1103 21 0.9283898932301895 1103 22 1.0436271230138132 1103 23 0.9969706750350156 1103 24 0.9679027352536238 1103 25 1.0216594729363706 1104 1 0.75597025713882 1104 2 0.8681519816504917 1104 3 0.8155004478170262 1104 4 0.638687236447673 1104 5 0.7276131894139581 1104 6 0.6920374111106966 1104 7 0.6342076767006191 1104 8 0.7174903453816128 1104 9 0.7498044232137033 1104 10 0.7192405499599488 1104 11 0.8063153275160033 1104 12 0.8924913247809343 1104 13 0.7289995755642522 1104 14 0.9898937416741518 1104 15 1.0112862501466253 1104 16 1.0123611095571334 1104 17 0.905405623824322 1104 18 0.5999339909878145 1104 19 0.9207765591009647 1104 20 0.9843877891140238 1104 21 0.9299602663816486 1104 22 0.8593758193044891 1104 23 0.9178179228325973 1104 24 1.1337562830353107 1104 25 0.9569784267481154 1105 1 0.5180646172784193 1105 2 0.5198096745618557 1105 3 0.5459732457482147 1105 4 0.32592905409747885 1105 5 0.4580421136906807 1105 6 0.6070429148936753 1105 7 0.6767345481624361 1105 8 0.6165616518158288 1105 9 0.8066684219092437 1105 10 0.5562306353201194 1105 11 0.8270007192127338 1105 12 0.823027296168398 1105 13 0.8280537355755015 1105 14 0.8327540298791547 1105 15 0.9201082171902613 1105 16 0.8861732246046827 1105 17 0.618356827223387 1105 18 0.5753359559978293 1105 19 0.8436535837808705 1105 20 0.8971648912580008 1105 21 0.7780403103520328 1105 22 0.7854882093281503 1105 23 0.9711278676312531 1105 24 0.9043809598125705 1105 25 0.7153026466060197 1106 1 0.18489426959619948 1106 2 0.5123251085223899 1106 3 -1.3343615182632291 1106 4 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24 -1.038592563844226 2213 25 -1.0220332945688 2214 1 -1.3778096931077641 2214 2 -0.6872164448653173 2214 3 -0.861874251545192 2214 4 -0.9275428394799548 2214 5 -1.2257189800968007 2214 6 -1.8635041733315134 2214 7 -1.3548402024096724 2214 8 -1.1980636715166142 2214 9 -0.9612796893106363 2214 10 -1.3749052258948993 2214 11 -1.4095561062622934 2214 12 -1.1133914444719792 2214 13 -1.410464153865874 2214 14 -1.0524417199603988 2214 15 -1.2383749241424908 2214 16 -1.1663923656917845 2214 17 -1.3320376545695112 2214 18 -1.263806880770339 2214 19 -1.289095212028613 2214 20 -1.4310342361495714 2214 21 -1.5783106196136742 2214 22 -1.9648997896086584 2214 23 -2.0367209262333716 2214 24 -1.6555549895692891 2214 25 -2.26329614887932 2215 1 -1.5610047589115261 2215 2 -1.0488556282436328 2215 3 -1.1079091801888161 2215 4 -1.2291083714802724 2215 5 -1.679126442199916 2215 6 -1.5435766273170088 2215 7 -1.9909802081148946 2215 8 -1.743923452159006 2215 9 -1.8381617966275243 2215 10 -1.677539724181571 2215 11 -1.4567243316913903 2215 12 -1.7037748565945208 2215 13 -1.8374810852306283 2215 14 -1.9631488741458156 2215 15 -1.9157263623634933 2215 16 -2.0963780087209045 2215 17 -2.161414623076915 2215 18 -1.9213260948112918 2215 19 -2.118999972368151 2215 20 -2.1747653581415634 2215 21 -1.8434181325472707 2215 22 -2.0186102389712843 2215 23 -2.428655783385622 2215 24 -2.3062273489372984 2215 25 -2.6495613525358266 2216 1 -1.74577910687157 2216 2 -1.1718211430214183 2216 3 -1.1167515162355421 2216 4 -1.3855596710949172 2216 5 -2.3478515204536414 2216 6 -2.4834294299390116 2216 7 -2.369252129204778 2216 8 -2.4113240219265 2216 9 -4.231151557780635 2216 10 -2.292291388608163 2216 11 -2.4768573072083555 2216 12 -3.0401325866336784 2216 13 -2.857365345626878 2216 14 -3.1073930391640516 2216 15 -3.0727339220528904 2216 16 -2.228745691830156 2216 17 -2.6102122395197966 2216 18 -2.9292412191018755 2216 19 -3.2294514773848775 2216 20 -2.4628145296121104 2216 21 -3.204026479908972 2216 22 -2.5921248694038326 2216 23 -3.6369792559309566 2216 24 -2.9711730290191056 2216 25 -3.044601115698306 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 78950.0 3 2.0 4 1458.0 5 11842.0 6 13561.0 7 10243.0 8 8265.0 9 7178.0 10 6235.0 11 5636.0 12 5051.0 13 4754.0 14 4659.0 15 4544.0 16 4861.0 17 4822.0 18 5475.0 19 6568.0 20 8239.0 21 10873.0 22 13610.0 23 16511.0 24 19688.0 25 22583.0 26 25057.0 27 26978.0 28 31030.0 29 37185.0 30 46063.0 31 59065.0 32 74176.0 33 95548.0 34 124566.0 35 164231.0 36 224422.0 37 347631.0 38 1120656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.79056705306448 18.819138201306743 13.354498544498641 27.035796201130136 2 13.694782146211558 20.13994243877047 40.04854606972981 26.116729345288164 3 17.633615940012266 24.707267901628438 29.373332699787536 28.28578345857176 4 11.85505825572188 16.394743787941632 36.59303947814939 35.15715847818709 5 13.136287821851289 38.50480068873791 34.690948474002816 13.667963015407983 6 32.70765959565931 36.09262078987835 17.647424995359604 13.552294619102737 7 28.883849655606973 31.406719578616126 21.11001899774178 18.59941176803512 8 26.2409600560088 36.377504138042376 19.712547510158384 17.668988295790438 9 27.165440441667265 14.615596375251247 19.287193082356314 38.931770100725174 10 15.867203908456654 28.78201723680081 33.12460016174589 22.226178692996644 11 35.199231399212394 21.94565648552162 23.93472775454245 18.920384360723535 12 24.030045852275062 24.441475854314866 30.609117744313902 20.91936054909617 13 29.258108231418774 21.42619052826145 25.60720768550311 23.708493554816673 14 21.5186608878273 21.57461433034023 26.86783261474886 30.038892167083613 15 23.62818409789392 30.401681844749888 22.77187623734374 23.198257820012444 16 22.890099737928693 27.39395539131582 26.874337751465905 22.841607119289577 17 22.118157466871384 28.17542253499884 27.03760207051953 22.668817927610245 18 21.95788902128173 26.865959789445103 29.16833833577171 22.007812853501456 19 24.034020341017833 26.24544808078492 27.017040334396864 22.70349124380038 20 24.408246430701734 26.379325556650663 27.473435281324726 21.738992731322877 21 23.75301210951919 25.83976161775967 26.638114388334866 23.769111884386273 22 24.06736268998307 27.09706244980601 26.653922577774765 22.181652282436158 23 22.90590208338989 26.86183930720575 27.29841762511047 22.933840984293887 24 23.619003882036758 26.20103339999457 27.632591010686912 22.547371707281762 25 23.054155468483714 26.615743212468367 27.519855094758494 22.810246224289425 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 113.0 1 113.0 2 298.5 3 484.0 4 484.0 5 484.0 6 1187.5 7 1891.0 8 1891.0 9 1891.0 10 2540.5 11 3190.0 12 3190.0 13 3190.0 14 5167.5 15 7145.0 16 7145.0 17 7145.0 18 14623.0 19 22101.0 20 22101.0 21 22101.0 22 37494.5 23 52888.0 24 52888.0 25 52888.0 26 80823.0 27 108758.0 28 108758.0 29 108758.0 30 143465.0 31 178172.0 32 178172.0 33 178172.0 34 204633.0 35 231094.0 36 231094.0 37 231094.0 38 255487.0 39 279880.0 40 279880.0 41 279880.0 42 299230.5 43 318581.0 44 318581.0 45 318581.0 46 336612.0 47 354643.0 48 354643.0 49 354643.0 50 347121.5 51 339600.0 52 339600.0 53 339600.0 54 307297.0 55 274994.0 56 274994.0 57 274994.0 58 239005.5 59 203017.0 60 203017.0 61 203017.0 62 169757.5 63 136498.0 64 136498.0 65 136498.0 66 106851.0 67 77204.0 68 77204.0 69 77204.0 70 57372.0 71 37540.0 72 37540.0 73 37540.0 74 26534.0 75 15528.0 76 15528.0 77 15528.0 78 10826.0 79 6124.0 80 6124.0 81 6124.0 82 4070.0 83 2016.0 84 2016.0 85 2016.0 86 1318.5 87 621.0 88 621.0 89 621.0 90 373.5 91 126.0 92 126.0 93 126.0 94 67.0 95 8.0 96 8.0 97 8.0 98 4.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08558880573829583 2 0.04245506399177141 3 0.01787184754182917 4 0.004373701086186042 5 0.015421066760776649 6 0.059572825139430574 7 0.07167591176585919 8 0.04539600092903444 9 0.016816126589991164 10 0.043133741746524415 11 0.004675335643854045 12 1.1311295912550109E-4 13 0.005957282513943058 14 4.1474751679350396E-4 15 0.013799781013311134 16 0.010029349042461097 17 0.004826152922688046 18 0.0066359602686960634 19 0.001432764148923014 20 5.278604759190051E-4 21 1.885215985425018E-4 22 2.2622591825100218E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2652216.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.83015401227327 #Duplication Level Percentage of deduplicated Percentage of total 1 76.52771189735769 43.490816533337146 2 13.316217825675981 15.135254197882892 3 4.447286917898376 7.5822000144279835 4 1.9591715271837327 4.453600785052485 5 1.0583212987717912 3.0072281201834987 6 0.628479588652297 2.142995509000811 7 0.4202161395453788 1.6716663550164754 8 0.2890671457432529 1.3142184329981843 9 0.19750837336491556 1.010198814933755 >10 1.057152061477532 10.662493899589096 >50 0.06074651956572222 2.3739995599987775 >100 0.032925560036505 3.4592526858107577 >500 0.003530034237548483 1.434946355034039 >1k 0.0015985060698053978 2.0077893551956305 >5k 6.66044195752249E-5 0.2533393815384276 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6693 0.2523550118089929 No Hit TATCAACGCAGAGTACTTTTTTTTT 4760 0.17947256181246174 No Hit GTCCTACAGTGGACATTTCTAAATT 4735 0.1785299538197492 No Hit CTGTAGGACGTGGAATATGGCAAGA 4143 0.15620899655231701 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3883 0.14640587342810693 No Hit CTTTAGGACGTGAAATATGGCGAGG 3796 0.14312559761346738 No Hit GGTATCAACGCAGAGTACTTTTTTT 3751 0.14142890322658486 No Hit GTCCTACAGTGTGCATTTCTCATTT 3189 0.12023907555040766 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 3.7704319708500366E-5 0.0 0.0 0.0 0.0 7 3.7704319708500366E-5 0.0 0.0 0.0 0.0 8 3.7704319708500366E-5 0.0 0.0 0.0 0.0 9 3.7704319708500366E-5 0.0 0.0 0.0 0.0 10 3.7704319708500366E-5 0.0 0.0 0.0 0.0 11 3.7704319708500366E-5 0.0 0.0 0.0 0.0 12 3.7704319708500366E-5 0.0 0.0 0.0 0.0 13 3.7704319708500366E-5 0.0 0.0 3.7704319708500366E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1850 0.0 13.677376 1 GTCGCGT 60 4.1189237E-4 12.657453 16 TCGCGTC 70 1.0996737E-4 12.20448 17 TAGGACC 935 0.0 11.899471 4 CGCGTCC 90 7.536215E-6 11.601352 18 TCTACAC 470 0.0 11.5348625 3 TGGACCG 95 1.34623915E-5 11.010891 5 GTAATAC 140 8.20728E-9 10.869946 3 CTACACT 490 0.0 10.673823 4 GGCGAGG 1160 0.0 10.637002 19 TGGCGAG 2480 0.0 10.52542 18 GTCCTAA 1330 0.0 10.513772 1 GTATCAA 4070 0.0 10.353858 1 ATAGGAC 295 0.0 10.317237 3 TCCAACG 215 1.8189894E-12 10.154249 18 AGGACGT 2435 0.0 10.114728 5 GGACGTG 2420 0.0 10.059157 6 GTAGGAC 2490 0.0 10.045969 3 TGTAGGA 2560 0.0 9.994193 2 AGGACCT 1735 0.0 9.975286 5 >>END_MODULE