FastQCFastQC Report
Thu 26 May 2016
SRR1512874_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512874_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315791
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT114210.4931792204046047No Hit
GTCCTACAGTGGACATTTCTAAATT68360.2951907145333927No Hit
TATCAACGCAGAGTACTTTTTTTTT67800.2927725343090115No Hit
GGTATCAACGCAGAGTACTTTTTTT63750.27528390947196874No Hit
CTGTAGGACGTGGAATATGGCAAGA62980.27195891166344455No Hit
GTCCTAAAGTGTGTATTTCTCATTT58340.2519225612328574No Hit
CTTTAGGACGTGAAATATGGCGAGG53910.2327930283864131No Hit
GTCCTACAGTGTGCATTTCTCATTT33370.1440976322992878No Hit
ACGCAGAGTACTTTTTTTTTTTTTT31160.1345544567709262No Hit
CTGAAGGACCTGGAATATGGCGAGA27940.12064992048073422No Hit
GTACTTTTTTTTTTTTTTTTTTTTT25740.11114992674209373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGATA551.1306964E-515.5426782
TAGGACC8450.013.1514964
TGCACCG957.410017E-812.9976785
GGTATCA18750.012.804751
GCACCGT1051.9990694E-812.6649516
CCAACGA1457.2759576E-1212.44605219
TCCAACG1850.011.80921218
CTAGACT1459.822543E-1111.7909974
AGGACCG1302.6248017E-911.6902195
TTTTACG658.0380583E-411.6902192
GTATCAA45250.011.3485941
GTCCTAT2550.011.2086391
ATAAGAC2050.011.1199643
GTCCTAC30000.011.1152341
ACCGGGC600.00588305411.0818338
CACCTTT8150.011.07188114
CGTGCGC1658.0035534E-1110.9374410
TACACCG700.001494103910.8552045
GGCGAGG16000.010.74495419
GCGTGCG1701.4006218E-1010.615759
Produced by FastQC (version 0.11.5)