##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512874_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2315791
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.699000039295427	31.0	30.0	34.0	16.0	34.0
2	28.771845559465426	31.0	30.0	34.0	16.0	34.0
3	28.870382949065785	31.0	30.0	34.0	18.0	34.0
4	32.242551681045484	35.0	33.0	37.0	22.0	37.0
5	32.4017098261458	35.0	33.0	37.0	23.0	37.0
6	32.29622966839408	36.0	33.0	37.0	22.0	37.0
7	32.18803251243312	36.0	33.0	37.0	19.0	37.0
8	32.11356335696961	36.0	33.0	37.0	19.0	37.0
9	33.34829179317132	38.0	33.0	39.0	17.0	39.0
10	33.209693361793015	38.0	33.0	39.0	17.0	39.0
11	33.27825611205847	38.0	33.0	39.0	17.0	39.0
12	33.17445529410901	38.0	33.0	39.0	17.0	39.0
13	33.14847583395911	38.0	33.0	39.0	17.0	39.0
14	34.016460034605885	39.0	33.0	41.0	16.0	41.0
15	34.185928695637905	39.0	34.0	41.0	17.0	41.0
16	34.1278699157221	39.0	34.0	41.0	16.0	41.0
17	33.93195543121119	39.0	33.0	41.0	11.0	41.0
18	33.91558176018475	39.0	33.0	41.0	10.0	41.0
19	34.025175847043194	39.0	34.0	41.0	10.0	41.0
20	34.01488994473163	39.0	34.0	41.0	10.0	41.0
21	33.9214726199385	39.0	34.0	41.0	10.0	41.0
22	33.92466850419576	39.0	34.0	41.0	2.0	41.0
23	33.602728830019636	39.0	33.0	41.0	2.0	41.0
24	33.675049259626626	39.0	34.0	41.0	2.0	41.0
25	33.396410125093325	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.5732107806587692
1101	2	0.28111406453912124
1101	3	0.5896499271079279
1101	4	0.6211805685931395
1101	5	0.4645110481452903
1101	6	0.5074957712975774
1101	7	0.2655667026724444
1101	8	0.5270969377076256
1101	9	0.6261009646110836
1101	10	0.6517732278501001
1101	11	0.7152674805393247
1101	12	0.47865985896415
1101	13	0.9515870700160107
1101	14	0.9416386424622374
1101	15	0.8179002735664298
1101	16	0.6861040348470482
1101	17	0.20432509309299007
1101	18	0.6424488957801984
1101	19	0.14033929171807813
1101	20	-0.4851784911776491
1101	21	0.538254959252491
1101	22	0.5979445354057802
1101	23	0.11526138532564545
1101	24	0.5937478041880837
1101	25	0.22978250211244955
1102	1	0.936611345322671
1102	2	0.9588828591835608
1102	3	0.9105258757970134
1102	4	1.057998216453825
1102	5	0.9888507721461579
1102	6	0.8429665946975788
1102	7	0.8462310745835069
1102	8	0.9730038854910248
1102	9	1.2053882269541845
1102	10	1.1296092992891928
1102	11	1.004801922558265
1102	12	1.0995466813555979
1102	13	0.9732536190035432
1102	14	1.1129182180095611
1102	15	1.0856215260933624
1102	16	1.172369071809726
1102	17	1.2767173505647804
1102	18	1.2625456981054057
1102	19	1.1944445137401658
1102	20	1.3624443916435496
1102	21	1.3079350983334024
1102	22	1.075992579989311
1102	23	1.195521064107588
1102	24	0.48674959333788337
1102	25	0.876194266991206
1103	1	0.3983722421804252
1103	2	0.5166487169719893
1103	3	0.6992182972082972
1103	4	0.8407997174442698
1103	5	0.6264133565243952
1103	6	0.14227267927225284
1103	7	0.06516133215164643
1103	8	0.410353142755568
1103	9	0.502359877638682
1103	10	0.66508540762797
1103	11	0.46761393544804974
1103	12	0.41828041725092646
1103	13	0.6708291422410255
1103	14	0.6373896447693213
1103	15	0.6546771076133453
1103	16	0.5496656306407388
1103	17	0.2675881680127574
1103	18	0.6252380036374916
1103	19	0.4905370935394018
1103	20	0.747714370977441
1103	21	0.5802088848312863
1103	22	0.4748301814907805
1103	23	-0.14278982797605977
1103	24	0.13352198795505643
1103	25	0.3120309606736882
1104	1	1.1342483101695109
1104	2	1.0322258126970993
1104	3	1.1142580242032523
1104	4	1.2776394873873258
1104	5	1.0284487774977364
1104	6	0.9699537663249487
1104	7	1.0424441143607837
1104	8	0.799808348660676
1104	9	1.2512286090914415
1104	10	1.2366248297603875
1104	11	0.9743771836009856
1104	12	1.227723652881636
1104	13	1.2718382501709868
1104	14	1.4969383533509202
1104	15	1.2502707012742746
1104	16	1.2959374248624087
1104	17	1.5645186722907525
1104	18	1.2969760823049583
1104	19	0.8859198706603095
1104	20	1.0454732216931149
1104	21	1.2454730544711197
1104	22	1.1031195275153607
1104	23	1.3323039377360004
1104	24	1.2810978780286106
1104	25	0.9029285070261608
1105	1	0.5138264586118382
1105	2	0.5413048878007984
1105	3	0.6425952355974509
1105	4	0.8221709972683158
1105	5	0.4913133746206668
1105	6	0.7996171557331806
1105	7	0.6818292168366327
1105	8	0.6867475997282071
1105	9	0.4107857803642574
1105	10	0.6689030250449406
1105	11	0.5715987693799534
1105	12	0.7089161184118709
1105	13	0.6490414045534507
1105	14	0.8645170007647565
1105	15	0.7532704704575224
1105	16	0.7086500817668835
1105	17	1.0405852238884776
1105	18	0.8268000251302894
1105	19	0.6109208041104068
1105	20	0.7998899256977836
1105	21	0.7392681570827406
1105	22	0.7882001945757722
1105	23	0.9403503624523992
1105	24	1.051686817154085
1105	25	1.32494108484196
1106	1	0.38216369796043637
1106	2	0.41606451763073693
1106	3	0.37754913190545736
1106	4	0.5550486673417652
1106	5	0.3424697804857004
1106	6	0.512298675658478
1106	7	0.3974006680253055
1106	8	0.5799380168166763
1106	9	0.6655654487458733
1106	10	0.3642656142014715
1106	11	0.1322906483350863
1106	12	0.5276005944280158
1106	13	0.4101656031269414
1106	14	0.7206378306889931
1106	15	0.47863541709072166
1106	16	0.4942632914944767
1106	17	0.7062911321410326
1106	18	0.5205466160341317
1106	19	0.6317847615279106
1106	20	0.8121313255304514
1106	21	0.6302325499400467
1106	22	0.6457586230171088
1106	23	0.9911739191913185
1106	24	0.6888425562230225
1106	25	0.8926542573584015
1107	1	0.6712702911146806
1107	2	0.778049795572457
1107	3	0.6094745267538855
1107	4	0.7570588976361563
1107	5	0.719777947572851
1107	6	0.7457896207146533
1107	7	0.5141725581050309
1107	8	0.7536169300220052
1107	9	0.7702762549216118
1107	10	0.8848284690388368
1107	11	0.778612183341636
1107	12	0.9231194259226143
1107	13	0.712651179143748
1107	14	0.9531867506756555
1107	15	0.7999268148125296
1107	16	0.7614178566530683
1107	17	1.1969789629189194
1107	18	1.0997577120582775
1107	19	0.9866794267710759
1107	20	1.041981967430857
1107	21	0.8091135577699973
1107	22	0.7515160606748807
1107	23	1.1293306222922226
1107	24	0.8891368867540379
1107	25	1.1312340384352666
1108	1	0.6612905453899103
1108	2	0.664560531307103
1108	3	0.6940834919175032
1108	4	0.7442866637771246
1108	5	0.6230874144916783
1108	6	0.6942091713839034
1108	7	0.6224141174973923
1108	8	0.6532335727115353
1108	9	0.9133780722705396
1108	10	0.8173328786858747
1108	11	0.5348075903889082
1108	12	0.6473078882515324
1108	13	0.6979497722777808
1108	14	0.6919748295088652
1108	15	0.7534288945605354
1108	16	0.5075425225128853
1108	17	0.7184795645961444
1108	18	0.9087327709977373
1108	19	0.8433337554217815
1108	20	0.7312174463887615
1108	21	0.8527810001470328
1108	22	0.9197962187509532
1108	23	1.2403647390169112
1108	24	1.0120557028857675
1108	25	1.1100589444815867
1109	1	0.8403469264480954
1109	2	0.8175621977423191
1109	3	0.9157638140949764
1109	4	1.1232798591666509
1109	5	0.9498586962580973
1109	6	1.006620642012983
1109	7	0.9478689154421218
1109	8	0.8383321701285666
1109	9	0.8584870849076722
1109	10	0.95681350637647
1109	11	0.7981973973601626
1109	12	0.8072815030272551
1109	13	0.9933795736942486
1109	14	1.0746980623931819
1109	15	0.8620783876864948
1109	16	0.5653831002104255
1109	17	1.040850068692798
1109	18	1.0236730570691108
1109	19	1.0210771243983103
1109	20	0.9119777574566683
1109	21	0.9053320347255678
1109	22	0.9892067172928236
1109	23	1.2803334933565864
1109	24	1.048235400150034
1109	25	0.5885876210542804
1110	1	0.40035932655831985
1110	2	0.5046702164802639
1110	3	0.38496194030054554
1110	4	0.283690440594043
1110	5	0.20769281033911113
1110	6	0.362662251271729
1110	7	0.2640855040340213
1110	8	0.33625032940108923
1110	9	0.48019554453842517
1110	10	0.4597619770295438
1110	11	0.3928295274235438
1110	12	0.3577003511947936
1110	13	0.3052533681279783
1110	14	0.391463011414956
1110	15	0.3763619368736002
1110	16	0.5076426758511019
1110	17	0.5423077705390327
1110	18	-0.19956061611909348
1110	19	0.25204433299571605
1110	20	0.3709209925076493
1110	21	0.48997518710914534
1110	22	0.4813877589020805
1110	23	0.5905332729101858
1110	24	0.46609982561159313
1110	25	0.7347256756147686
1111	1	0.1544285789187576
1111	2	0.15021222687418856
1111	3	0.2581726035964067
1111	4	0.112886995087635
1111	5	0.24256352105070533
1111	6	0.16191223816202438
1111	7	0.04152094218795099
1111	8	0.2674533587088703
1111	9	0.35970535197755993
1111	10	0.3767452545661385
1111	11	0.08624472971318653
1111	12	-0.19740484383621038
1111	13	0.14129539646150135
1111	14	0.16396726861908206
1111	15	0.11656733144810971
1111	16	0.2049377987649308
1111	17	-0.19032297461658487
1111	18	0.13121807274483643
1111	19	0.06051085632961417
1111	20	0.3196612188777479
1111	21	0.14652838353116238
1111	22	-0.22145207914476828
1111	23	0.11968801434540666
1111	24	0.24605494902221636
1111	25	0.1998226736303721
1112	1	0.31822672599928836
1112	2	0.27197379238416985
1112	3	0.14837887253626647
1112	4	0.2959148798861193
1112	5	0.27242350482673316
1112	6	-0.058383075100884696
1112	7	-0.2643232180435682
1112	8	0.0487701700459624
1112	9	-0.06601847011249617
1112	10	0.010214665565314363
1112	11	-0.1859592996399897
1112	12	-0.024005444396230757
1112	13	-0.013352945545257455
1112	14	0.028712820711945142
1112	15	-0.18008807017933037
1112	16	0.05261729215117583
1112	17	0.28832683673112314
1112	18	0.014710063002731033
1112	19	0.06384169341960444
1112	20	-0.11423526070212375
1112	21	-0.09694696908791656
1112	22	-0.20416202040284048
1112	23	0.022605957803079946
1112	24	0.0551614846524231
1112	25	0.036857166878036196
1113	1	-0.12149347045978232
1113	2	0.08339231604592356
1113	3	0.06814825097836774
1113	4	0.11542014360780684
1113	5	-0.05416362625090443
1113	6	-0.36768462683943426
1113	7	-0.41339128130346126
1113	8	-0.15860966037412183
1113	9	-0.12726366605684092
1113	10	-0.10815951584638128
1113	11	-0.25604871969064646
1113	12	-0.36810123973714326
1113	13	-0.2913629629286163
1113	14	-0.03494944696717539
1113	15	-0.2853520324265446
1113	16	-0.271896486367595
1113	17	-0.1172179238473845
1113	18	-0.31247082915442803
1113	19	-0.670745405960588
1113	20	-0.5877355947634797
1113	21	-0.33990322003291595
1113	22	-0.8017799290634002
1113	23	-4.547245696028831
1113	24	-2.104171102989067
1113	25	-1.1762822592184818
1114	1	-0.8246748333778271
1114	2	-1.0413465620987132
1114	3	-0.8365873048738486
1114	4	-0.8615261912738355
1114	5	-0.8206213799012936
1114	6	-1.3484902726552583
1114	7	-1.3404789999967797
1114	8	-1.2002267702888005
1114	9	-1.4358373782744032
1114	10	-0.8982887194723901
1114	11	-1.114476039030123
1114	12	-2.1069332543657957
1114	13	-1.3539711674998216
1114	14	-3.203084702011367
1114	15	-1.9182190721560488
1114	16	-1.9814559828546834
1114	17	-1.8054202367003143
1114	18	-1.8090776720361674
1114	19	-1.843270156378999
1114	20	-1.470447178699395
1114	21	-1.3704184526038716
1114	22	-1.5888827246583048
1114	23	-5.419254807714783
1114	24	-2.799205704765569
1114	25	-2.8924548106062637
1115	1	-1.4314815436909818
1115	2	-1.4014844040817565
1115	3	-1.137860401885387
1115	4	-1.189536854465441
1115	5	-1.113842785425387
1115	6	-1.2190808093702294
1115	7	-1.6240487890089916
1115	8	-1.9825291152091324
1115	9	-3.3042069126077074
1115	10	-3.8313239356742486
1115	11	-2.6025119320947354
1115	12	-2.8902947779168997
1115	13	-2.2085587349443614
1115	14	-2.916374567367747
1115	15	-2.47225652126118
1115	16	-2.413728798707851
1115	17	-2.3938549581229474
1115	18	-4.911540559874801
1115	19	-2.9766500519189947
1115	20	-3.212046270661986
1115	21	-2.86878728776027
1115	22	-2.5169374211188362
1115	23	-3.000581726471914
1115	24	-2.7284906550570653
1115	25	-3.0702874812091494
1116	1	-0.7145347579400152
1116	2	-0.34727353668019845
1116	3	-0.8850659490518176
1116	4	-0.31543953527051727
1116	5	-0.5142852359046586
1116	6	-2.38428648134159
1116	7	-0.9605197280349813
1116	8	-1.5432513278403164
1116	9	-2.607491516171155
1116	10	-1.7919644909153654
1116	11	-1.4381370384763699
1116	12	-1.075793149018292
1116	13	-2.523753104056645
1116	14	-5.195423582926356
1116	15	-3.0715730807145825
1116	16	-2.126913954108204
1116	17	-2.817422256997009
1116	18	-1.7215924316209836
1116	19	-1.301786202201619
1116	20	-2.011454394200417
1116	21	-2.6576234137860553
1116	22	-2.1998280004589574
1116	23	-1.1602475406286317
1116	24	-2.0778288854056903
1116	25	-2.3734640338104214
1201	1	0.5278534690476882
1201	2	0.6504976440930044
1201	3	0.9784622729492973
1201	4	0.9299329559971241
1201	5	0.367168960869904
1201	6	0.7887448060141438
1201	7	0.9607069497544174
1201	8	0.9534077735016382
1201	9	1.237675802558151
1201	10	1.0862697436547535
1201	11	1.185641421785867
1201	12	1.0016461439112376
1201	13	1.2466334265584536
1201	14	1.1817757012416905
1201	15	1.1899600393294136
1201	16	1.1485642575236028
1201	17	1.4584976406613919
1201	18	1.2830028040885963
1201	19	1.3863664526914192
1201	20	1.313229574845444
1201	21	1.520240438331264
1201	22	1.3540729574789268
1201	23	1.6226902831210523
1201	24	1.520837900876991
1201	25	1.503165986858292
1202	1	0.8924441889140375
1202	2	0.7993049790999862
1202	3	0.8926990445293228
1202	4	1.05753766885109
1202	5	0.850378813313263
1202	6	1.0510500701437024
1202	7	1.1822819817974093
1202	8	1.002478186360598
1202	9	1.314923389308774
1202	10	1.0963614312420447
1202	11	1.1472289532424753
1202	12	1.2969090023425167
1202	13	1.3017289804897914
1202	14	1.7707364945458792
1202	15	1.4055882407909053
1202	16	1.2038889169297136
1202	17	1.465778927404898
1202	18	1.385438903484662
1202	19	1.5350059686947546
1202	20	1.3269283117388397
1202	21	1.60398734185123
1202	22	1.4461280366141338
1202	23	1.6018959347929638
1202	24	1.4723921560496436
1202	25	1.865171325590893
1203	1	1.0830077134996436
1203	2	1.1942029808044694
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2211	16	-0.6584242185155702
2211	17	-0.22922673275287764
2211	18	-0.5548383502044345
2211	19	-0.4334993382174588
2211	20	-0.5526111289223721
2211	21	-0.37573358533316537
2211	22	-0.41687527954476167
2211	23	-0.25637985654176276
2211	24	-0.6287151306834815
2211	25	-0.09105039566132689
2212	1	-0.39558322549597946
2212	2	-0.369065279292748
2212	3	-0.666406155285113
2212	4	-0.44483037250473245
2212	5	-0.5851098172319951
2212	6	-0.7517261717833108
2212	7	-0.6788570652615604
2212	8	-0.5071040191371381
2212	9	-0.5393793429622207
2212	10	-0.8528057668339954
2212	11	-0.9424334883062571
2212	12	-0.5223782115720823
2212	13	-0.5947081263461911
2212	14	-0.46128668542586837
2212	15	-0.8594344091244182
2212	16	-0.8321927273937106
2212	17	-0.6613545647349
2212	18	-0.5881078083766056
2212	19	-0.792341906163827
2212	20	-0.6095569932510543
2212	21	-0.6175624132494733
2212	22	-0.5410419972487475
2212	23	-0.4630014768901063
2212	24	-0.6071675694033729
2212	25	-0.4625456044353484
2213	1	-0.31055023887664035
2213	2	-0.12271064517243957
2213	3	-0.3144965250252767
2213	4	-0.19432765178320466
2213	5	-0.18215564335363155
2213	6	-0.04688873221908807
2213	7	-0.2055591479875325
2213	8	-0.08400416026071511
2213	9	-0.5841150053220261
2213	10	-0.19181107622807758
2213	11	-0.22732667786497984
2213	12	-0.30001594272940224
2213	13	-0.338368644695727
2213	14	-0.5216449029575685
2213	15	-0.5118373237288196
2213	16	-0.34920933390975506
2213	17	-0.33689476716779865
2213	18	-0.3476031250092646
2213	19	-0.32588611589137173
2213	20	-0.21273993143736192
2213	21	-0.25516724973937244
2213	22	-0.43316242652249315
2213	23	-0.3300639538456025
2213	24	-0.15762143501670778
2213	25	0.09948666556201147
2214	1	-2.8180601973619943
2214	2	-1.5133740127244089
2214	3	-1.7792819945960119
2214	4	-3.638723656557275
2214	5	-2.0154020780949935
2214	6	-1.743069125144796
2214	7	-1.5916010444845874
2214	8	-1.3005533006953982
2214	9	-1.2290635281094495
2214	10	-1.2600455816209042
2214	11	-1.2342409657426145
2214	12	-1.0284699829619868
2214	13	-1.4321590322318336
2214	14	-1.3192764932997534
2214	15	-1.6929366584201375
2214	16	-1.5445975335137092
2214	17	-3.5639159717976163
2214	18	-3.3206100927811697
2214	19	-2.347528801682106
2214	20	-2.1269631307105357
2214	21	-2.0778541645683113
2214	22	-2.917998654614159
2214	23	-1.9674044511366873
2214	24	-3.0471600443059934
2214	25	-2.9693949344003023
2215	1	-1.495050975523327
2215	2	-3.2483571969643776
2215	3	-1.723293278809102
2215	4	-3.324725205040256
2215	5	-1.8493581603163278
2215	6	-1.6897183584090385
2215	7	-1.6686229155098289
2215	8	-1.1401699701017378
2215	9	-3.5398706651375456
2215	10	-1.70116261950524
2215	11	-1.403869884245914
2215	12	-1.4023519448536135
2215	13	-1.5049341659813997
2215	14	-1.1735975908292176
2215	15	-1.4581027782868574
2215	16	-1.3927630383318004
2215	17	-1.7761469771865066
2215	18	-1.3572002171818056
2215	19	-1.4734643840118906
2215	20	-1.3712732825143945
2215	21	-2.0458670971246775
2215	22	-1.7798729836778193
2215	23	-1.7827915443235263
2215	24	-1.7114806425339921
2215	25	-2.8011630873318047
2216	1	-2.4343764098865215
2216	2	-2.325443859391182
2216	3	-3.6239259624459095
2216	4	-4.247941389912501
2216	5	-3.613701097471953
2216	6	-2.680489532334242
2216	7	-3.9943427568178436
2216	8	-2.296235645210718
2216	9	-2.7941862391489707
2216	10	-1.9175091587622681
2216	11	-2.0510937543137366
2216	12	-2.409919119056724
2216	13	-2.0768283333205666
2216	14	-2.9023568610741144
2216	15	-2.5290483209981254
2216	16	-2.5748575480665643
2216	17	-2.505845519422124
2216	18	-1.97375634458432
2216	19	-2.617918537384451
2216	20	-3.1147010507613864
2216	21	-3.066706108844869
2216	22	-2.6183667459141837
2216	23	-2.486283106463336
2216	24	-2.961898839024883
2216	25	-2.6102414695571596
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	132163.0
3	2.0
4	1107.0
5	7049.0
6	9124.0
7	8288.0
8	7032.0
9	6492.0
10	5636.0
11	5278.0
12	4900.0
13	4766.0
14	4631.0
15	4788.0
16	4888.0
17	5238.0
18	5748.0
19	7094.0
20	9238.0
21	11977.0
22	15085.0
23	17790.0
24	20756.0
25	23177.0
26	25614.0
27	28176.0
28	31275.0
29	36753.0
30	44749.0
31	55233.0
32	68917.0
33	87323.0
34	113254.0
35	148423.0
36	203246.0
37	315854.0
38	834727.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.24360091993546	18.711189139631575	12.784078600062045	24.26113134037092
2	14.546166732382334	20.15947961828194	42.527653484233205	22.76670016510252
3	19.1552781082721	25.85259257499822	29.138802576488143	25.85332674024154
4	12.144496393463578	16.593522486113258	38.503997809561405	32.75798331086176
5	12.62127536444691	39.36821284433782	34.89564866259698	13.114863128618287
6	31.18337796950853	37.07142032928921	18.4378880095147	13.307313691687558
7	27.958554820865206	32.243312156964436	21.81809931284844	17.980033709321923
8	25.26263644061972	37.21766825785251	20.5179977772677	17.001697524260074
9	27.599446797476013	14.792851586135361	20.068674907079263	37.53902670930936
10	16.358753860796096	28.735387756619218	33.55117170245008	21.35468668013461
11	34.07759441495167	22.479966495933656	25.070307972040183	18.372131117074495
12	24.428401495215933	24.90832120170546	31.16093627350971	19.5023410295689
13	29.38039068574212	22.566490436415137	25.74848876040537	22.304630117437373
14	20.99601245793545	23.0170989745456	27.59654856753624	28.39033999998271
15	22.84508578684547	32.21927271564269	23.36817419972184	21.567467297790003
16	21.42233232731813	28.184474634024692	28.772697470641802	21.62049556801537
17	21.066782048350515	29.30324476405901	28.022620458088916	21.60735272950156
18	20.998944852666245	27.061395223783308	31.20547448994368	20.73418543360677
19	23.41843908481889	26.63241343509312	28.235964337104015	21.713183142983976
20	24.011241316592958	27.005957794060187	28.84974464127026	20.1330562480766
21	22.988266309397755	26.545670635520885	27.59552261382544	22.870540441255915
22	23.439519465104077	28.492164421363675	27.575859484348292	20.492456629183952
23	21.836197398546737	28.14087250216442	28.708311415385772	21.31461868390307
24	23.196982447314067	26.53446072310445	29.331021847174803	20.937534982406685
25	22.359857577661927	27.329079795754126	29.06518700495574	21.245875621628212
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	494.0
1	494.0
2	1080.5
3	1667.0
4	1667.0
5	1667.0
6	3156.0
7	4645.0
8	4645.0
9	4645.0
10	5679.0
11	6713.0
12	6713.0
13	6713.0
14	8966.5
15	11220.0
16	11220.0
17	11220.0
18	19930.0
19	28640.0
20	28640.0
21	28640.0
22	44867.5
23	61095.0
24	61095.0
25	61095.0
26	90756.5
27	120418.0
28	120418.0
29	120418.0
30	154514.5
31	188611.0
32	188611.0
33	188611.0
34	208163.5
35	227716.0
36	227716.0
37	227716.0
38	240020.5
39	252325.0
40	252325.0
41	252325.0
42	264400.0
43	276475.0
44	276475.0
45	276475.0
46	289010.5
47	301546.0
48	301546.0
49	301546.0
50	288337.0
51	275128.0
52	275128.0
53	275128.0
54	242622.0
55	210116.0
56	210116.0
57	210116.0
58	180860.0
59	151604.0
60	151604.0
61	151604.0
62	125404.0
63	99204.0
64	99204.0
65	99204.0
66	76744.5
67	54285.0
68	54285.0
69	54285.0
70	40413.0
71	26541.0
72	26541.0
73	26541.0
74	18840.5
75	11140.0
76	11140.0
77	11140.0
78	7708.5
79	4277.0
80	4277.0
81	4277.0
82	2820.5
83	1364.0
84	1364.0
85	1364.0
86	893.5
87	423.0
88	423.0
89	423.0
90	265.5
91	108.0
92	108.0
93	108.0
94	65.5
95	23.0
96	23.0
97	23.0
98	18.0
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33694750519368977
2	0.011227265327484217
3	0.010190902374177982
4	0.011788628593858427
5	0.01278180975744357
6	0.08195903689063477
7	0.08165676436258713
8	0.10458629470448758
9	0.08597494333469644
10	0.09279766611062915
11	0.08817721461047219
12	0.09841129877437127
13	0.08472267143278474
14	0.0900340315684792
15	0.08778857850298236
16	0.08817721461047219
17	0.09974993425572515
18	0.10234084163899074
19	0.09664084539580645
20	0.09426584696114632
21	0.08381585384864178
22	0.10229765984926964
23	0.09664084539580645
24	0.0918908485264862
25	0.09107039452178543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2315791.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.59140143290716
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.07426267272464	44.388148017302164
2	13.279980123120799	15.296253325833556
3	4.343901924516926	7.505141985600969
4	1.9462425748462167	4.483473496551333
5	1.0309777969663816	2.9687728086752565
6	0.5846931550353017	2.020397892402665
7	0.3871372007314413	1.5607041755855477
8	0.26943114730208545	1.2413533890242519
9	0.17198125397413563	0.8914177292903284
>10	0.8266826353585441	8.203339747541552
>50	0.044922044451964925	1.7803878794355437
>100	0.03292264760161916	3.757136945680941
>500	0.004224506689153749	1.7086278586650225
>1k	0.0021122533445761503	2.068835181085979
>5k	4.526257166948894E-4	1.6298175724660202
>10k+	7.543761944914824E-5	0.49619199485883714
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	11421	0.4931792204046047	No Hit
GTCCTACAGTGGACATTTCTAAATT	6836	0.2951907145333927	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6780	0.2927725343090115	No Hit
GGTATCAACGCAGAGTACTTTTTTT	6375	0.27528390947196874	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6298	0.27195891166344455	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5834	0.2519225612328574	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5391	0.2327930283864131	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3337	0.1440976322992878	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	3116	0.1345544567709262	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2794	0.12064992048073422	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2574	0.11114992674209373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	4.318178972109314E-5
11	0.0	0.0	0.0	0.0	4.318178972109314E-5
12	0.0	0.0	0.0	0.0	8.636357944218628E-5
13	0.0	0.0	0.0	0.0	8.636357944218628E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGATA	55	1.1306964E-5	15.542678	2
TAGGACC	845	0.0	13.151496	4
TGCACCG	95	7.410017E-8	12.997678	5
GGTATCA	1875	0.0	12.80475	1
GCACCGT	105	1.9990694E-8	12.664951	6
CCAACGA	145	7.2759576E-12	12.446052	19
TCCAACG	185	0.0	11.809212	18
CTAGACT	145	9.822543E-11	11.790997	4
AGGACCG	130	2.6248017E-9	11.690219	5
TTTTACG	65	8.0380583E-4	11.690219	2
GTATCAA	4525	0.0	11.348594	1
GTCCTAT	255	0.0	11.208639	1
ATAAGAC	205	0.0	11.119964	3
GTCCTAC	3000	0.0	11.115234	1
ACCGGGC	60	0.005883054	11.081833	8
CACCTTT	815	0.0	11.071881	14
CGTGCGC	165	8.0035534E-11	10.93744	10
TACACCG	70	0.0014941039	10.855204	5
GGCGAGG	1600	0.0	10.744954	19
GCGTGCG	170	1.4006218E-10	10.61575	9
>>END_MODULE