FastQCFastQC Report
Thu 26 May 2016
SRR1512874_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512874_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2315791
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT145840.6297632212924223No Hit
TATCAACGCAGAGTACTTTTTTTTT107050.46226105896430203No Hit
GGTATCAACGCAGAGTACTTTTTTT85620.36972248359199944No Hit
GTCCTACAGTGGACATTTCTAAATT63620.27472254620559455No Hit
CTGTAGGACGTGGAATATGGCAAGA60210.2599975559107018No Hit
CTTTAGGACGTGAAATATGGCGAGG55830.241083932012863No Hit
GTCCTAAAGTGTGTATTTCTCATTT53590.23141121111533813No Hit
ACGCAGAGTACTTTTTTTTTTTTTT50590.2184566741990102No Hit
GTCCTACAGTGTGCATTTCTCATTT30770.1328703669718036No Hit
GTACTTTTTTTTTTTTTTTTTTTTT30670.13243854907459265No Hit
CTGAAGGACCTGGAATATGGCGAGA30160.13023627779881689No Hit
GAGTACTTTTTTTTTTTTTTTTTTT24990.10791129251301176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2200.014.670855518
GGTATCA21250.014.0109331
TAGGACC8250.013.2545774
CCAACGT957.480048E-812.98832919
CCAACGA1750.011.93213619
GGCGAGG14900.011.91211519
ACCGTGC855.321228E-511.1772978
TGGTCGG700.001494996510.85442710
TGTAGGA32100.010.7848032
GTATTAG1158.676907E-710.7529381
AACGCTG803.7403393E-410.6973075
CACCTTT8800.010.67998114
GTATCAA46350.010.6512181
CGAATAT909.5869276E-510.54812912
TGGCGAG35150.010.5326718
AGGACCT19750.010.4956985
GTCCTAC31150.010.4435871
GGACCTG19500.010.4352076
AGTCGTC2100.010.4055318
CTGTAGG32650.010.4007991
Produced by FastQC (version 0.11.5)