##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512874_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2315791
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.98134805774787	31.0	30.0	34.0	26.0	34.0
2	30.01793512454276	31.0	30.0	34.0	26.0	34.0
3	30.176739610785255	31.0	30.0	34.0	26.0	34.0
4	33.72526622652908	37.0	35.0	37.0	30.0	37.0
5	33.30742843374035	37.0	35.0	37.0	27.0	37.0
6	33.16045403060984	37.0	35.0	37.0	26.0	37.0
7	32.971286268924956	37.0	35.0	37.0	26.0	37.0
8	32.89923831641111	37.0	35.0	37.0	25.0	37.0
9	34.18963369319597	39.0	35.0	39.0	25.0	39.0
10	34.09258262079782	39.0	35.0	39.0	25.0	39.0
11	34.07232042960699	39.0	35.0	39.0	24.0	39.0
12	33.945859103865594	39.0	35.0	39.0	23.0	39.0
13	33.92090866576474	39.0	35.0	39.0	23.0	39.0
14	34.96413752363663	39.0	35.0	41.0	22.0	41.0
15	34.96812752100686	39.0	35.0	41.0	23.0	41.0
16	34.969283497517694	39.0	35.0	41.0	23.0	41.0
17	34.96923988391008	40.0	35.0	41.0	23.0	41.0
18	34.903100495683766	40.0	35.0	41.0	22.0	41.0
19	34.92107621110886	40.0	35.0	41.0	21.0	41.0
20	34.901801155631055	40.0	35.0	41.0	21.0	41.0
21	34.86373295344873	40.0	35.0	41.0	19.0	41.0
22	34.82707679579029	40.0	35.0	41.0	19.0	41.0
23	34.67272867024701	40.0	35.0	41.0	18.0	41.0
24	34.62954860779751	40.0	35.0	41.0	18.0	41.0
25	34.563658810315786	40.0	35.0	41.0	18.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7167640730416274
1101	2	0.5649134142078722
1101	3	0.5711324352948743
1101	4	0.5830866764417877
1101	5	0.6851173626956069
1101	6	0.8190060065838907
1101	7	0.8514451765105946
1101	8	0.6100669893734576
1101	9	0.9736826963608607
1101	10	0.611106440479027
1101	11	0.969714447563419
1101	12	1.077526141855408
1101	13	0.9966563936222101
1101	14	1.0913185784912258
1101	15	0.8035759723923022
1101	16	1.0462744173923753
1101	17	1.0435805632290212
1101	18	0.9896698975679712
1101	19	0.8698464552350913
1101	20	0.9859370854858724
1101	21	1.0852311622491797
1101	22	0.9472674896276629
1101	23	1.070117453972209
1101	24	1.058188883162586
1101	25	1.1617934910609904
1102	1	0.9496885737536935
1102	2	0.999174649368527
1102	3	0.7800289172716184
1102	4	0.8718475257480236
1102	5	0.9756604291505013
1102	6	1.036858033655264
1102	7	1.025173708766495
1102	8	1.1342676083531273
1102	9	1.3057874947380839
1102	10	1.3325991986158598
1102	11	1.3364035724820624
1102	12	1.3053115183307895
1102	13	1.3996428111396781
1102	14	1.3205033695650599
1102	15	1.4327710418653155
1102	16	1.4924189589633343
1102	17	1.4440175760459013
1102	18	0.9418283953060822
1102	19	1.2505994293584664
1102	20	1.4399094870798024
1102	21	1.2609789026026448
1102	22	1.1302242761178078
1102	23	1.3232965048162981
1102	24	1.2868880123395314
1102	25	1.370992522691111
1103	1	0.8882748858772871
1103	2	0.9575706151167616
1103	3	0.6867660367215009
1103	4	0.6463282642296448
1103	5	0.8803159353490102
1103	6	0.7355826677119879
1103	7	0.8566679770321812
1103	8	0.9395666727763512
1103	9	0.9532171047720368
1103	10	0.8363057073320732
1103	11	0.8928246993895996
1103	12	1.1364455855460491
1103	13	1.1797680789255836
1103	14	1.2481747917132964
1103	15	1.1768811560802348
1103	16	1.1806659844594236
1103	17	1.1085197846976413
1103	18	1.170201699575017
1103	19	1.2084589364638987
1103	20	1.0303059092502167
1103	21	1.2713921420383514
1103	22	1.158781331698144
1103	23	1.172858100082749
1103	24	1.1893861551683358
1103	25	1.0850361216509157
1104	1	1.0546830446369349
1104	2	1.1460925190803195
1104	3	1.057874080521664
1104	4	0.953139850558351
1104	5	1.1545684615684806
1104	6	1.0489061091831218
1104	7	1.0369769187754017
1104	8	1.1689045820661619
1104	9	1.1870205818435267
1104	10	1.2850409374537008
1104	11	1.2832580957652269
1104	12	1.2919623469486439
1104	13	1.213229221672833
1104	14	1.531837954401226
1104	15	1.5414521191957462
1104	16	1.4113833212631022
1104	17	1.4214820912694819
1104	18	1.0862439801710124
1104	19	1.4954175898597768
1104	20	1.5024681219916616
1104	21	1.4999055857431358
1104	22	1.2951275434253944
1104	23	1.36350714250392
1104	24	1.4998884323664328
1104	25	1.4136143324743529
1105	1	0.7315550788113896
1105	2	0.7664600608140262
1105	3	0.7856813044071274
1105	4	0.6155654715572396
1105	5	0.7076270381079937
1105	6	0.8082792210681475
1105	7	0.811206313288757
1105	8	0.8077875308180538
1105	9	1.1329844284725823
1105	10	0.7525799980974099
1105	11	1.0684290104784395
1105	12	1.008005553807969
1105	13	1.108502697094245
1105	14	1.306236854966727
1105	15	1.2587815778625782
1105	16	1.2625637962025138
1105	17	1.0219569627927996
1105	18	0.8805238828206541
1105	19	1.224802132265566
1105	20	1.285588628702321
1105	21	1.1649155906310185
1105	22	1.1321545812318234
1105	23	1.4209249310078889
1105	24	1.2895838221412532
1105	25	1.1445963474367105
1106	1	0.1992780163659944
1106	2	0.41119757196581475
1106	3	-1.3845935149685573
1106	4	-0.21988077913035653
1106	5	0.01754479221855121
1106	6	0.1325741159755367
1106	7	0.2822812879805525
1106	8	0.29290480372602445
1106	9	0.5332634543262458
1106	10	0.360289091254721
1106	11	0.47671002285287756
1106	12	0.5747802137072924
1106	13	0.5165742522892387
1106	14	0.5813701275141412
1106	15	0.6474458419831706
1106	16	0.6763156856507209
1106	17	0.6842126057136682
1106	18	0.7315963613606797
1106	19	0.7347643490794553
1106	20	0.7150584738022303
1106	21	0.6706121036456736
1106	22	0.7427200925037951
1106	23	0.7289364803323082
1106	24	0.806187853888602
1106	25	0.7770944811010168
1107	1	0.7837556729327169
1107	2	0.9221487641030421
1107	3	0.7580165845217124
1107	4	0.5547787819595769
1107	5	0.73579022141476
1107	6	0.6472868837874586
1107	7	0.6429522129437828
1107	8	0.7797399271410228
1107	9	0.7714979987819888
1107	10	0.8960464707584137
1107	11	0.8981602521146712
1107	12	1.0176045471304107
1107	13	0.9336868259516606
1107	14	1.0234767545679588
1107	15	0.9944436095759457
1107	16	1.0810579659456963
1107	17	1.1246236160102043
1107	18	1.1493675653641802
1107	19	1.149333887663765
1107	20	1.084495203376008
1107	21	1.125550639754458
1107	22	1.11948558279866
1107	23	1.2943736422319745
1107	24	1.263199724553374
1107	25	1.3427429124941739
1108	1	0.6941831993715688
1108	2	0.6941014021756828
1108	3	0.6734586875341115
1108	4	0.6014441615421333
1108	5	0.8181611133848108
1108	6	0.721671695641362
1108	7	0.7832286730749658
1108	8	0.7133380122920201
1108	9	0.9458520174945306
1108	10	0.8276965610272953
1108	11	0.8858899118608434
1108	12	0.9739863737795531
1108	13	0.9863262825530512
1108	14	1.041212358428382
1108	15	0.9982854172509832
1108	16	1.0097789710891902
1108	17	1.0501835148979808
1108	18	1.1073666201185333
1108	19	1.095292824144778
1108	20	1.101900109657599
1108	21	1.0646344019428398
1108	22	1.0904998950355775
1108	23	1.247830812432234
1108	24	1.2071559304785282
1108	25	1.1588501473600346
1109	1	0.5608010302283937
1109	2	0.5485000968014297
1109	3	0.5943418981992998
1109	4	0.5731335102437001
1109	5	0.2717217299658401
1109	6	0.6228125094172299
1109	7	0.6657373524250545
1109	8	0.6990920742278632
1109	9	0.9671893317564368
1109	10	0.5300670651239514
1109	11	0.8223758165962991
1109	12	0.9343314647391452
1109	13	0.9098257965094447
1109	14	1.0729142053940777
1109	15	0.9743095805616306
1109	16	0.9146859284562225
1109	17	0.9920874171337815
1109	18	1.1264111972606017
1109	19	1.1153796176456794
1109	20	1.1881054977981336
1109	21	1.366816737729529
1109	22	1.2477106990905682
1109	23	1.2129301041972695
1109	24	1.2248784690148469
1109	25	1.0850152633161514
1110	1	0.6063411239181171
1110	2	0.4991132133624667
1110	3	0.4912776931841414
1110	4	0.5447055686619962
1110	5	0.6615095503020214
1110	6	0.720625956647865
1110	7	0.609563475323931
1110	8	0.7064260505298705
1110	9	0.7336125888520542
1110	10	0.8012629868654741
1110	11	0.8738321406525102
1110	12	0.8938643939207367
1110	13	0.8297541514281548
1110	14	0.8261341006820402
1110	15	0.8146926061885296
1110	16	0.6731138700379233
1110	17	0.819739662931525
1110	18	0.8280755676105613
1110	19	0.9824628361631795
1110	20	0.8654449852602468
1110	21	0.9622466082172707
1110	22	0.9769405144667047
1110	23	1.067760636024218
1110	24	1.1325582706130817
1110	25	1.0523795509987082
1111	1	0.3965182291297076
1111	2	0.2319205945402416
1111	3	0.2161425252281255
1111	4	0.4245774799465565
1111	5	0.4675580920888649
1111	6	0.4694139299427107
1111	7	0.3703170211540723
1111	8	0.43683463172862247
1111	9	0.5056919536247619
1111	10	0.35690140188837205
1111	11	0.38237345830203395
1111	12	0.5859854613542979
1111	13	0.5123988342699022
1111	14	0.33103492231553133
1111	15	0.47792609417037824
1111	16	0.2859145997654906
1111	17	0.3792541346656222
1111	18	0.27804341433464685
1111	19	0.01557008458794229
1111	20	0.28525071323525
1111	21	0.42122827395015605
1111	22	0.20619847757737375
1111	23	0.31792857824216014
1111	24	0.44736105925428404
1111	25	0.4505662718337575
1112	1	0.030748095773503792
1112	2	-0.29249037637339725
1112	3	-0.01192570646498936
1112	4	0.08260671260613606
1112	5	-0.02268373832166759
1112	6	-0.06286772242054184
1112	7	0.05543867544225378
1112	8	-0.057769511465380674
1112	9	0.026226048581193595
1112	10	-0.24198010092090527
1112	11	-0.17782098393236367
1112	12	0.2218617179158997
1112	13	0.005050819569319742
1112	14	-0.21883442133885467
1112	15	-0.3670708453920781
1112	16	-0.5106413152350129
1112	17	-0.26133232762440173
1112	18	-0.03505582367914428
1112	19	-0.0921546279867087
1112	20	-0.10834954577470057
1112	21	-0.13244472681333264
1112	22	0.1095748316951628
1112	23	0.1842276522788282
1112	24	0.13392564882678926
1112	25	0.0630119454478475
1113	1	-0.5580683944829659
1113	2	-0.4212758951123554
1113	3	-0.3622223146048249
1113	4	-1.8840850738144965
1113	5	-0.5985638500919563
1113	6	-0.3188250203010341
1113	7	-0.35197736647931777
1113	8	-0.29998320729485073
1113	9	-0.14226285565013796
1113	10	-0.36777912595558604
1113	11	-0.35547115747941405
1113	12	-0.09052417923961542
1113	13	-0.27885435667307945
1113	14	-0.3793024567644281
1113	15	-0.57125581385489
1113	16	-0.8577654901904737
1113	17	-0.8414810523222513
1113	18	-0.6545309606768441
1113	19	-0.44578569420033887
1113	20	-0.3191706008878583
1113	21	-0.29736823443851534
1113	22	-0.21875891353987242
1113	23	-0.14116268796707487
1113	24	-0.1507327662683835
1113	25	-0.3878566954555396
1114	1	-1.3237140580953444
1114	2	-2.596467565679493
1114	3	-1.7868730254146463
1114	4	-1.3726527145776117
1114	5	-1.4157124238942593
1114	6	-0.910739253279786
1114	7	-1.0084631250822333
1114	8	-1.0725847168022575
1114	9	-1.3172863924377722
1114	10	-1.472516763094312
1114	11	-1.4550649105445572
1114	12	-1.4939890242524996
1114	13	-1.5613490829804064
1114	14	-1.6716895844422694
1114	15	-1.7740599435932864
1114	16	-2.269734565068717
1114	17	-2.275084712155852
1114	18	-1.9718870030204627
1114	19	-2.4277692860519764
1114	20	-1.9042786386843318
1114	21	-2.151670878813981
1114	22	-1.8373532203980716
1114	23	-1.6597153768611008
1114	24	-1.636286890551169
1114	25	-2.2936110721176064
1115	1	-1.4860324394561815
1115	2	-1.5642161714166107
1115	3	-1.1004203961375794
1115	4	-0.8583880181022039
1115	5	-1.1582806940562165
1115	6	-1.0525910902555289
1115	7	-1.060940070839493
1115	8	-1.332933650706238
1115	9	-2.3479259607927254
1115	10	-2.101909118859325
1115	11	-2.5366856323628895
1115	12	-3.0297667990550536
1115	13	-3.0155797726812317
1115	14	-2.5780616149858844
1115	15	-2.776550947269172
1115	16	-2.6324281217816363
1115	17	-2.5177556274379214
1115	18	-2.7280602144292487
1115	19	-3.2348805102214797
1115	20	-3.2921537210607568
1115	21	-2.6076324032576537
1115	22	-2.589371535857765
1115	23	-2.586689324339531
1115	24	-3.1528677964761727
1115	25	-3.0656481612210094
1116	1	-2.589902315017895
1116	2	-2.77749881549407
1116	3	-2.8720262786747703
1116	4	-2.4208378320724435
1116	5	-3.0858269392610005
1116	6	-3.893827926552497
1116	7	-3.300419609993643
1116	8	-3.821628363517707
1116	9	-4.6511984237986255
1116	10	-3.729419937917182
1116	11	-4.543308549316379
1116	12	-6.294668180092039
1116	13	-5.6746218049911406
1116	14	-5.231318186001957
1116	15	-6.255383642373392
1116	16	-6.071886803457918
1116	17	-5.597738106185272
1116	18	-5.6709197090858225
1116	19	-6.029001056731108
1116	20	-7.541402038440186
1116	21	-6.72168322897339
1116	22	-5.915704138086728
1116	23	-6.521608287331954
1116	24	-6.441408906953544
1116	25	-5.650198281934575
1201	1	0.7838208034182763
1201	2	0.9617379566444058
1201	3	0.9704047071553887
1201	4	0.8873193200721374
1201	5	0.3261677898669788
1201	6	0.9289378365472061
1201	7	1.1727146714131322
1201	8	1.0791633158678735
1201	9	0.9852469066328524
1201	10	1.1688196094682368
1201	11	0.8367215566370021
1201	12	1.2741817119040206
1201	13	1.374152635706885
1201	14	1.3006213877145214
1201	15	1.1836000719835553
1201	16	1.165582991555958
1201	17	1.1134453700270655
1201	18	1.3769026191855787
1201	19	1.4368378903993886
1201	20	1.5936492605198538
1201	21	1.3088391279592528
1201	22	1.171213170739307
1201	23	1.3808655505479521
1201	24	0.975422231736566
1201	25	1.210157746596174
1202	1	1.0415801246365604
1202	2	1.0346549354181036
1202	3	1.0172682774176813
1202	4	0.8544895194120627
1202	5	0.9610064248583754
1202	6	0.9130007058738556
1202	7	1.0434016549232936
1202	8	0.9621384638255179
1202	9	1.2643573751187418
1202	10	1.293894855635564
1202	11	1.1318812873397093
1202	12	1.1678708699489633
1202	13	1.1745091565117534
1202	14	1.1909270785795059
1202	15	1.2170484120281415
1202	16	1.2072904780388853
1202	17	1.1783226230615043
1202	18	1.1914437045765496
1202	19	1.3361899940385982
1202	20	1.2473483151949551
1202	21	1.3593835182799197
1202	22	1.2645961234155578
1202	23	1.4187306779405873
1202	24	1.4666726422045997
1202	25	1.5922816935659796
1203	1	0.9638587625396831
1203	2	1.1192057930941388
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2211	15	-0.22910977792040654
2211	16	-0.15283044832263215
2211	17	-0.2893164681158211
2211	18	-0.35038290709901077
2211	19	-0.22371062405298403
2211	20	-0.3194515385696093
2211	21	-0.2398762823743752
2211	22	-0.32604692458234297
2211	23	-0.5061228662115838
2211	24	-0.2713159064086952
2211	25	-0.4405195232210062
2212	1	-0.3194164866703275
2212	2	-0.3693562207212473
2212	3	-0.24953816419598596
2212	4	-0.313299270390651
2212	5	-0.5951602389520616
2212	6	-0.3702855898374935
2212	7	-0.5544720761958928
2212	8	-0.32509100971690685
2212	9	-0.48120456737118644
2212	10	-0.6915996978317409
2212	11	-0.34710624475906826
2212	12	-0.12062000559420483
2212	13	-0.19494513190929297
2212	14	-0.1811856848364357
2212	15	-0.36247763995947935
2212	16	-0.42107668059109216
2212	17	-0.40701420629911667
2212	18	-0.4499860527249595
2212	19	-0.31590786285578787
2212	20	-0.26969015764123583
2212	21	-0.18165428890401358
2212	22	-0.15292914145481262
2212	23	-0.5668532455052002
2212	24	-0.5318249800578911
2212	25	-0.36547494409040127
2213	1	-0.5425104515796697
2213	2	-0.1494454502576481
2213	3	-0.21808412703957458
2213	4	-0.41793263699751293
2213	5	-0.24803007332706528
2213	6	-0.38483554851943325
2213	7	-0.72697221261636
2213	8	-0.6279130140214662
2213	9	-0.5285282993165765
2213	10	-0.5026846369821811
2213	11	-0.33946903516507376
2213	12	-0.6624978476244365
2213	13	-0.430658743596652
2213	14	-0.5096963411590636
2213	15	-0.7007500473601311
2213	16	-1.0499661335857269
2213	17	-0.8197420397823336
2213	18	-0.6109536099785942
2213	19	-0.5245408061823582
2213	20	-0.5505440201191547
2213	21	-0.49652018532515285
2213	22	-0.6666480658525487
2213	23	-0.7846937411893364
2213	24	-0.5412236718082397
2213	25	-0.4511385550391296
2214	1	-1.5247801070189126
2214	2	-0.8407065562591853
2214	3	-0.9629846619031923
2214	4	-1.0684346367016389
2214	5	-1.3553259557641937
2214	6	-2.0060418263939965
2214	7	-1.3967310284053092
2214	8	-1.3424337648532827
2214	9	-1.1733390696319788
2214	10	-1.458916354364483
2214	11	-1.4278133025360944
2214	12	-1.1271745611520743
2214	13	-1.5271360817213662
2214	14	-1.247409881783959
2214	15	-1.3435260146534063
2214	16	-1.232313714012534
2214	17	-1.2865657328654763
2214	18	-1.4446269840971269
2214	19	-1.364989160938194
2214	20	-1.70721911077964
2214	21	-1.6825852167746191
2214	22	-2.023201526988352
2214	23	-2.1161931993914465
2214	24	-1.8814325533755465
2214	25	-2.4012362450187936
2215	1	-1.716928818448281
2215	2	-1.1334209859742366
2215	3	-1.1902912482406833
2215	4	-1.331574694977256
2215	5	-1.7734781253425318
2215	6	-1.560282987055917
2215	7	-2.002648431622692
2215	8	-1.7608859205873557
2215	9	-1.73296741750503
2215	10	-1.6885794652914328
2215	11	-1.442879805276256
2215	12	-1.619907698460949
2215	13	-1.8809374518175552
2215	14	-2.062372163448991
2215	15	-1.8345829256179016
2215	16	-1.7903538617409183
2215	17	-1.9732408519926423
2215	18	-1.6342736764356687
2215	19	-2.0128482113770474
2215	20	-1.9757339547852126
2215	21	-1.6161581823886308
2215	22	-1.8174472559344466
2215	23	-2.338894216053532
2215	24	-2.266412300865184
2215	25	-2.4599750905754476
2216	1	-2.955971090586864
2216	2	-2.2201644689675817
2216	3	-2.291543343676775
2216	4	-2.7382518544121055
2216	5	-3.4904686604737094
2216	6	-3.8025951192968606
2216	7	-3.8043751956636704
2216	8	-3.8137265391796866
2216	9	-6.232204954893312
2216	10	-4.0916518511402735
2216	11	-4.11859537109299
2216	12	-4.561208766086754
2216	13	-4.518695116971767
2216	14	-4.697389060546822
2216	15	-4.70522303792081
2216	16	-4.057303512486726
2216	17	-4.596162562402927
2216	18	-4.95476093992529
2216	19	-4.674525023428231
2216	20	-4.274655818400607
2216	21	-5.055222633638234
2216	22	-4.629347907768725
2216	23	-5.802540960712282
2216	24	-4.979274760092139
2216	25	-5.167457111243888
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	78858.0
3	0.0
4	1283.0
5	11214.0
6	14069.0
7	10922.0
8	8687.0
9	7189.0
10	5999.0
11	5272.0
12	4691.0
13	4280.0
14	4070.0
15	3995.0
16	4163.0
17	4443.0
18	4836.0
19	5871.0
20	8048.0
21	10425.0
22	12825.0
23	15325.0
24	17786.0
25	20178.0
26	22011.0
27	23903.0
28	27036.0
29	32048.0
30	39852.0
31	50760.0
32	64238.0
33	82045.0
34	106920.0
35	140357.0
36	193628.0
37	303054.0
38	965510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.98650717881186	19.62725757328888	14.004917402930129	26.381317844969125
2	13.008102687317136	20.810951263353104	41.07065023234154	25.110295816988216
3	17.160167872615833	25.785169870173846	30.114148023151806	26.940514234058515
4	11.397119789399499	17.412457074946968	37.494558796094765	33.69586433955876
5	12.241477157391099	39.346695079741174	35.10127556507265	13.310552197795078
6	31.122987535803187	37.6183087631336	17.95597023663697	13.302733464426247
7	27.521272674395743	32.35183670329832	21.879168703078797	18.247721919227146
8	25.334954948338023	37.487562457740445	20.08433550148732	17.093147092434215
9	26.899225408869576	14.650069149124556	20.049790824977993	38.40091461702787
10	15.709579654763473	29.347308118523575	33.54531688444664	21.39779534226631
11	33.74601625454514	22.58146705474897	25.01381895442336	18.65869773628253
12	23.9184881504595	25.12606950728608	31.297062854685194	19.65837948756923
13	29.036161383837864	22.488747205424662	25.970754609834746	22.504336800902728
14	20.70681030700618	22.894060931416746	27.771717914969617	28.627410846607454
15	22.616648628478686	32.15208395056183	23.512497311547005	21.718770109412482
16	21.286727855106648	28.209186557660093	28.593626043421537	21.91045954381172
17	20.812959609826372	29.233009469759946	28.267205369292135	21.686825551121547
18	20.77770652364018	26.94464417195135	31.499856412116657	20.777792892291814
19	23.21301105946964	26.389342904878028	28.682117652443935	21.715528383208397
20	23.708726578045543	26.810494624695995	29.184083434667258	20.2966953625912
21	22.53944551943204	26.342253115460863	28.223944409443174	22.894356955663923
22	23.223389380538617	28.034801143110805	28.173026350448616	20.568783125901962
23	21.539767621516795	27.816240757477683	29.15569669283627	21.488294928169253
24	22.986400758963136	26.404930324023194	29.65060318482972	20.95806573218395
25	21.958712092260644	27.23251318018453	29.52119517036757	21.287579557187257
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	263.0
1	263.0
2	708.0
3	1153.0
4	1153.0
5	1153.0
6	2641.5
7	4130.0
8	4130.0
9	4130.0
10	5197.0
11	6264.0
12	6264.0
13	6264.0
14	8641.5
15	11019.0
16	11019.0
17	11019.0
18	20211.0
19	29403.0
20	29403.0
21	29403.0
22	46814.5
23	64226.0
24	64226.0
25	64226.0
26	95166.5
27	126107.0
28	126107.0
29	126107.0
30	158674.5
31	191242.0
32	191242.0
33	191242.0
34	210303.0
35	229364.0
36	229364.0
37	229364.0
38	241090.5
39	252817.0
40	252817.0
41	252817.0
42	265374.0
43	277931.0
44	277931.0
45	277931.0
46	289044.5
47	300158.0
48	300158.0
49	300158.0
50	286948.0
51	273738.0
52	273738.0
53	273738.0
54	240774.5
55	207811.0
56	207811.0
57	207811.0
58	178608.0
59	149405.0
60	149405.0
61	149405.0
62	123223.5
63	97042.0
64	97042.0
65	97042.0
66	74924.5
67	52807.0
68	52807.0
69	52807.0
70	38840.5
71	24874.0
72	24874.0
73	24874.0
74	17727.5
75	10581.0
76	10581.0
77	10581.0
78	7209.5
79	3838.0
80	3838.0
81	3838.0
82	2510.5
83	1183.0
84	1183.0
85	1183.0
86	764.0
87	345.0
88	345.0
89	345.0
90	213.5
91	82.0
92	82.0
93	82.0
94	44.5
95	7.0
96	7.0
97	7.0
98	4.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0849817621711113
2	0.03799997495456196
3	0.020036350430587217
4	0.005484087294578829
5	0.018352260631464584
6	0.060540869188972585
7	0.07219995241366772
8	0.052940874198060185
9	0.021806803809152037
10	0.04948633102037274
11	0.005656814453463201
12	2.159089486054657E-4
13	0.006218177719837412
14	3.0227252804765196E-4
15	0.014379535977124014
16	0.009154539420871745
17	0.004490906130993687
18	0.005872723402068666
19	0.0016409080094015392
20	6.477268458163971E-4
21	8.636357944218628E-5
22	2.159089486054657E-4
23	0.0
24	0.0
25	1.7272715888437255E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2315791.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.50526901395933
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.034927222861	42.9637401718458
2	13.964133163480225	15.780942018984021
3	4.562148318478825	7.7335625405168456
4	2.045430485504287	4.623103993310927
5	1.0080466240215853	2.84799728344766
6	0.6071019949077165	2.058267692470313
7	0.3866221288765779	1.5292331179244478
8	0.2552324693863241	1.1537583475017106
9	0.19786504548119196	1.0062375861036643
>10	0.84930325613157	8.228290006213603
>50	0.04861668550986605	1.898238313782275
>100	0.0335988038457479	3.77126167344778
>500	0.003908394255728158	1.4741178479993347
>1k	0.0024523258075150097	2.2362924629637755
>5k	4.598110889090644E-4	1.599869514487145
>10k+	1.532703629696881E-4	1.0950874290008505
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	14584	0.6297632212924223	No Hit
TATCAACGCAGAGTACTTTTTTTTT	10705	0.46226105896430203	No Hit
GGTATCAACGCAGAGTACTTTTTTT	8562	0.36972248359199944	No Hit
GTCCTACAGTGGACATTTCTAAATT	6362	0.27472254620559455	No Hit
CTGTAGGACGTGGAATATGGCAAGA	6021	0.2599975559107018	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5583	0.241083932012863	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5359	0.23141121111533813	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	5059	0.2184566741990102	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3077	0.1328703669718036	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3067	0.13243854907459265	No Hit
CTGAAGGACCTGGAATATGGCGAGA	3016	0.13023627779881689	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2499	0.10791129251301176	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCAACG	220	0.0	14.6708555	18
GGTATCA	2125	0.0	14.010933	1
TAGGACC	825	0.0	13.254577	4
CCAACGT	95	7.480048E-8	12.988329	19
CCAACGA	175	0.0	11.932136	19
GGCGAGG	1490	0.0	11.912115	19
ACCGTGC	85	5.321228E-5	11.177297	8
TGGTCGG	70	0.0014949965	10.854427	10
TGTAGGA	3210	0.0	10.784803	2
GTATTAG	115	8.676907E-7	10.752938	1
AACGCTG	80	3.7403393E-4	10.697307	5
CACCTTT	880	0.0	10.679981	14
GTATCAA	4635	0.0	10.651218	1
CGAATAT	90	9.5869276E-5	10.548129	12
TGGCGAG	3515	0.0	10.53267	18
AGGACCT	1975	0.0	10.495698	5
GTCCTAC	3115	0.0	10.443587	1
GGACCTG	1950	0.0	10.435207	6
AGTCGTC	210	0.0	10.405531	8
CTGTAGG	3265	0.0	10.400799	1
>>END_MODULE