FastQCFastQC Report
Thu 26 May 2016
SRR1512873_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512873_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544841
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT63600.24991738187179474No Hit
GTCCTAAAGTGTGTATTTCTCATTT53910.21184034680359204No Hit
CTGTAGGACGTGGAATATGGCAAGA50610.19887293548005555No Hit
GTATCAACGCAGAGTACTTTTTTTT47720.18751662677550385No Hit
CTTTAGGACGTGAAATATGGCGAGG44440.17462780582362514No Hit
GTCCTACAGTGTGCATTTCTCATTT40840.16048153892522166No Hit
CTGTAGGACCTGGAATATGGCGAGA29640.11647093079685529No Hit
TATCAACGCAGAGTACTTTTTTTTT28060.11026229143588932No Hit
GGTATCAACGCAGAGTACTTTTTTT26970.10597911618053937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11000.014.8523784
CGATACT602.5730671E-514.247924
GTCCTAT2500.012.573531
GGCGAGG13650.012.03853619
GGTATCA17700.011.7318511
ATTTTCG658.036337E-411.690615
TAGAAAT7400.011.2956494
GTAAACG855.3336393E-511.17483913
GTCCTAA14350.011.018931
AGGACCT20850.010.9792135
TCGTCTC1408.378265E-910.85555816
TGTAGGA32500.010.7553542
GTAGGAC31900.010.7492153
TGGCGAG31450.010.72180618
ACGATAC909.533712E-510.5540153
GGACCTG21300.010.5242856
GTCCTAC33000.010.5068071
TTAGGAC19600.010.3709353
GTATCAA36500.010.30831051
GTATAAT5400.010.2309871
Produced by FastQC (version 0.11.5)