##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512873_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2544841
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.69510983200915	31.0	30.0	34.0	16.0	34.0
2	28.76242837961193	31.0	30.0	34.0	16.0	34.0
3	28.867328449989607	31.0	30.0	34.0	18.0	34.0
4	32.25200434919117	35.0	33.0	37.0	22.0	37.0
5	32.418845813942795	35.0	33.0	37.0	23.0	37.0
6	32.323596641204695	36.0	33.0	37.0	22.0	37.0
7	32.2481942879732	36.0	33.0	37.0	22.0	37.0
8	32.178022516927385	35.0	33.0	37.0	19.0	37.0
9	33.470682844232705	38.0	33.0	39.0	18.0	39.0
10	33.30236152278276	38.0	33.0	39.0	17.0	39.0
11	33.41496384253476	38.0	33.0	39.0	18.0	39.0
12	33.2976299894571	38.0	33.0	39.0	17.0	39.0
13	33.296894776530245	38.0	33.0	39.0	17.0	39.0
14	34.126599657896115	39.0	33.0	41.0	17.0	41.0
15	34.319632935810134	39.0	34.0	41.0	17.0	41.0
16	34.27411457140151	39.0	34.0	41.0	17.0	41.0
17	34.020764362095704	39.0	33.0	41.0	15.0	41.0
18	34.01953049326068	39.0	33.0	41.0	15.0	41.0
19	34.12841352367398	39.0	34.0	41.0	16.0	41.0
20	34.0849428313989	39.0	34.0	41.0	11.0	41.0
21	33.97491198860754	39.0	34.0	41.0	10.0	41.0
22	33.98733437570363	39.0	34.0	41.0	9.0	41.0
23	33.64661839384072	39.0	33.0	41.0	2.0	41.0
24	33.70970013450742	39.0	34.0	41.0	2.0	41.0
25	33.42542500690613	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8009012947551497
1101	2	0.5028148832403971
1101	3	0.8249943356148854
1101	4	0.9456719323082226
1101	5	0.9242255149907805
1101	6	0.8641955267287145
1101	7	0.6905240411551219
1101	8	0.9218727586326381
1101	9	0.9492627487373397
1101	10	0.9378110097333519
1101	11	1.0080001737740432
1101	12	0.9609536754834096
1101	13	1.2921318605354273
1101	14	1.3437301293845394
1101	15	1.1856271847487037
1101	16	1.1461471183195258
1101	17	0.49959361656993195
1101	18	1.0099427333750697
1101	19	0.4048612745066862
1101	20	-0.07455147655609551
1101	21	0.9889948232443473
1101	22	1.0430474258475755
1101	23	0.4808341647742296
1101	24	1.0859505549704238
1101	25	0.6510289430704432
1102	1	1.1107964189531714
1102	2	1.197969304602232
1102	3	1.0823277947885295
1102	4	1.2501992315535446
1102	5	1.1193358606485688
1102	6	1.1290396619044998
1102	7	1.1373151200159697
1102	8	1.1551960626557403
1102	9	1.3603052494966477
1102	10	1.4692893223881143
1102	11	1.2905175152954769
1102	12	1.4581206435935883
1102	13	1.2612898358478901
1102	14	1.4999625896578408
1102	15	1.4904093241630676
1102	16	1.466500642026503
1102	17	1.6971484373348318
1102	18	1.6064422558544251
1102	19	1.4585861048084539
1102	20	1.6539219428153586
1102	21	1.8036053030249732
1102	22	1.569088313205917
1102	23	1.612101090081552
1102	24	1.0585335713220374
1102	25	1.427871825440043
1103	1	0.8306077534950695
1103	2	0.9768569583613029
1103	3	1.0926796689813614
1103	4	1.2123486321717962
1103	5	1.0124733810675934
1103	6	0.617200014377751
1103	7	0.5438694593570546
1103	8	0.7351370862674926
1103	9	0.8368090295600368
1103	10	1.019460067418919
1103	11	0.8392773772248461
1103	12	0.9058456209248007
1103	13	0.9903561738175881
1103	14	1.0999287131906854
1103	15	1.0912746947641168
1103	16	0.997543493453378
1103	17	0.7817559344221507
1103	18	1.1313506755060416
1103	19	1.0886553468327023
1103	20	1.3461736587907396
1103	21	1.169772586942024
1103	22	1.133130043009693
1103	23	0.5281587461697086
1103	24	0.6107906880462863
1103	25	0.958376971884995
1104	1	0.9140923354801487
1104	2	0.8875189886401138
1104	3	0.832429554343971
1104	4	0.967352301087594
1104	5	0.7838704564249994
1104	6	0.6873840625654424
1104	7	0.9158985593379967
1104	8	0.6517102773725654
1104	9	1.1769346607148137
1104	10	1.0725805413192475
1104	11	0.8656991534993992
1104	12	1.0443318724872697
1104	13	1.0909061476703954
1104	14	1.416312071009422
1104	15	1.032616832456732
1104	16	1.1144266339414344
1104	17	1.4486855011057997
1104	18	1.1245553916597046
1104	19	0.7414060989214946
1104	20	0.8702942484968972
1104	21	0.8689312289913786
1104	22	0.7078363609709726
1104	23	1.122349136154945
1104	24	0.9376256522685082
1104	25	0.4451719908838996
1105	1	0.7497346327125918
1105	2	0.8151039083862877
1105	3	0.9109583230743077
1105	4	1.0934121315147252
1105	5	0.8342117351537368
1105	6	0.9984009665274769
1105	7	0.9222602081408482
1105	8	0.848156179361581
1105	9	0.695443699268651
1105	10	0.8926066704719133
1105	11	0.865797804381927
1105	12	1.0766173734737734
1105	13	1.0350084246394147
1105	14	1.1726603886119449
1105	15	1.0863975608735217
1105	16	0.9933046801207084
1105	17	1.3470350985035466
1105	18	1.315567496389619
1105	19	0.8989488920745927
1105	20	1.2028810346111172
1105	21	0.9793535614181934
1105	22	0.9918789141624558
1105	23	1.2004699701548418
1105	24	1.2824824751768489
1105	25	1.4106068546390347
1106	1	0.5477981367965405
1106	2	0.49780887223475645
1106	3	0.4605282911599886
1106	4	0.5961708268141237
1106	5	0.3913176293857177
1106	6	0.5798275545803406
1106	7	0.6050445070175385
1106	8	0.5358078625263261
1106	9	0.6793837652609653
1106	10	0.45228103624245364
1106	11	0.19769859953527913
1106	12	0.34372579200211106
1106	13	0.5669362658251131
1106	14	0.8092275258795709
1106	15	0.5887713009791824
1106	16	0.794291860301378
1106	17	0.8854224337481327
1106	18	0.8288588182193735
1106	19	0.7981564433268176
1106	20	0.8658333795021207
1106	21	0.8559695618509267
1106	22	0.6663564749709536
1106	23	0.9330883866998008
1106	24	0.6435253701841503
1106	25	0.9450571891083044
1107	1	0.757176323298232
1107	2	0.8477400866631868
1107	3	0.9142687335460238
1107	4	0.9645208404804677
1107	5	0.7924940946343781
1107	6	0.9005397777360642
1107	7	0.5490649530748257
1107	8	0.7912544795772831
1107	9	0.7302253294973085
1107	10	0.9670601004157646
1107	11	0.8798141996389575
1107	12	0.9716465312553737
1107	13	0.6579064449600907
1107	14	1.0056983318508017
1107	15	0.7215327491877801
1107	16	0.9000859856908221
1107	17	1.1697887441485761
1107	18	1.106176624705995
1107	19	0.8837039593305605
1107	20	0.9824415649851375
1107	21	0.807356468587983
1107	22	0.8034144999374959
1107	23	1.1688458903281855
1107	24	0.9441521055825959
1107	25	1.1155473216062575
1108	1	0.8618314232099777
1108	2	0.8564468771501659
1108	3	0.880579148543017
1108	4	1.0599653392713435
1108	5	0.8926180172976146
1108	6	0.9133557943204949
1108	7	0.9582026063019811
1108	8	0.8695586221588698
1108	9	1.0400878199590338
1108	10	1.1050403457135474
1108	11	0.84484202946431
1108	12	1.0109201923640754
1108	13	0.9853110327852974
1108	14	1.1036027530784764
1108	15	1.1219631921357376
1108	16	0.7104122865849263
1108	17	1.1197947291862178
1108	18	1.1706657651610328
1108	19	1.0977119939137623
1108	20	1.0649843496084657
1108	21	1.0417535114276575
1108	22	1.0620235987779338
1108	23	1.323920554572375
1108	24	1.2222979145329944
1108	25	1.4463718966745773
1109	1	0.7850259515173299
1109	2	0.7565884143531925
1109	3	0.9094150978294451
1109	4	0.9651988462780849
1109	5	0.8665126862068675
1109	6	0.9267261946087189
1109	7	0.8929907504642856
1109	8	0.7103182069471004
1109	9	0.8756205947818998
1109	10	0.9584014001216445
1109	11	0.8704106979108133
1109	12	0.8423139917671207
1109	13	0.9802089904394933
1109	14	1.1175048396954992
1109	15	0.918722855345905
1109	16	0.5976094080922252
1109	17	1.0277149069282174
1109	18	1.09314130582122
1109	19	1.0347873845530273
1109	20	0.9066289990771068
1109	21	0.9025722040512676
1109	22	0.8802351985452361
1109	23	1.2265257276501202
1109	24	1.0254192874060948
1109	25	0.7590172478036976
1110	1	0.8224950007860592
1110	2	0.7904985994936169
1110	3	0.641324384212556
1110	4	0.7664633727565118
1110	5	0.5514500865065699
1110	6	0.577889834683269
1110	7	0.4936649974864764
1110	8	0.5705186548934762
1110	9	0.7093982937841332
1110	10	0.6650523832941175
1110	11	0.7317619849062282
1110	12	0.6858027456633735
1110	13	0.7245571138807634
1110	14	0.7602955348717515
1110	15	0.7045249962502851
1110	16	0.845171573631859
1110	17	0.9036108444377291
1110	18	0.30011097186662994
1110	19	0.6588577884030045
1110	20	0.7048293360215823
1110	21	0.93281019570464
1110	22	0.8795049134672936
1110	23	1.0022920435865927
1110	24	0.8256835435411887
1110	25	0.9451128276006386
1111	1	0.3685056342906101
1111	2	0.3502733133802529
1111	3	0.45167492940494824
1111	4	0.43393566256457206
1111	5	0.43863761466733564
1111	6	0.3670058119744297
1111	7	0.3034297987841228
1111	8	0.4441944231191215
1111	9	0.4756107836462604
1111	10	0.5623291247557702
1111	11	0.20494753054919812
1111	12	-0.15835153026705484
1111	13	0.2888411546124985
1111	14	0.2758925430721675
1111	15	0.34707703734247275
1111	16	0.4939433137418021
1111	17	0.0720383473664441
1111	18	0.28883585246499166
1111	19	0.3483594555202387
1111	20	0.543005419647784
1111	21	0.41450254498823114
1111	22	0.15004469899491113
1111	23	0.3720117876615703
1111	24	0.37415610369257735
1111	25	0.4752759347467119
1112	1	0.2426111842578358
1112	2	0.1888511588221391
1112	3	0.11987896085667771
1112	4	0.24330883314156182
1112	5	0.24160390042131752
1112	6	0.0978439709065384
1112	7	-0.051120944765884246
1112	8	0.07954686552155721
1112	9	-0.021783121146718543
1112	10	-0.012464424196494406
1112	11	0.037211106416066286
1112	12	0.050879122782205854
1112	13	0.2033682157246659
1112	14	0.06544544643634254
1112	15	-0.22380987599611757
1112	16	0.021650195242010284
1112	17	0.3617179396901946
1112	18	0.01574478080446795
1112	19	0.22371824143281316
1112	20	-0.14502751827341598
1112	21	0.06797307938364838
1112	22	-0.03260755280015104
1112	23	0.12939584491023481
1112	24	0.22225078511243623
1112	25	0.24588639187213346
1113	1	-0.29800870484436714
1113	2	-0.19980065071004205
1113	3	-0.07915132372271216
1113	4	-0.06362764684729427
1113	5	-0.19985505766349831
1113	6	-0.4223186126729388
1113	7	-0.5030240492732858
1113	8	-0.2903586303054979
1113	9	-0.4561585803725521
1113	10	-0.39805911797842697
1113	11	-0.4830086959074009
1113	12	-0.5848724684286779
1113	13	-0.45418504902247037
1113	14	-0.11561560947533422
1113	15	-0.4828825436193398
1113	16	-0.5013257340736459
1113	17	-0.2844982634834494
1113	18	-0.5857128016085298
1113	19	-1.0242023416146822
1113	20	-0.6020488848423042
1113	21	-0.3544374863571491
1113	22	-0.9533114559722335
1113	23	-4.496737971748917
1113	24	-1.9740415371553937
1113	25	-1.0910701292088518
1114	1	-0.8421553353529418
1114	2	-0.8433289041157401
1114	3	-0.7816605754394352
1114	4	-0.7890974274317877
1114	5	-0.704789285245166
1114	6	-1.3219187152429086
1114	7	-1.3492666431311449
1114	8	-1.1525612116327189
1114	9	-1.4850008160548427
1114	10	-1.0455318729896916
1114	11	-1.2536581038833035
1114	12	-2.0157719028728067
1114	13	-1.3006477100065652
1114	14	-3.2028836188459167
1114	15	-1.8970014436216829
1114	16	-1.8869364037062937
1114	17	-1.8960785054753586
1114	18	-1.7835158301367358
1114	19	-1.7658294531677186
1114	20	-1.2714527564106675
1114	21	-1.218731537516419
1114	22	-1.4802320409283212
1114	23	-5.54371135745285
1114	24	-2.827840409116142
1114	25	-2.7586499656320242
1115	1	-1.9487122279334486
1115	2	-1.812076496156667
1115	3	-1.5894935501030574
1115	4	-1.5859954953591533
1115	5	-1.609420579834481
1115	6	-1.699520416856636
1115	7	-2.2654590901047627
1115	8	-2.5874160225185854
1115	9	-3.98522358231822
1115	10	-4.47522927627227
1115	11	-3.1911000163781544
1115	12	-3.4992730961948197
1115	13	-2.861827531421742
1115	14	-3.5014244517417303
1115	15	-3.2769075420324434
1115	16	-3.353922122946436
1115	17	-3.2719370530649634
1115	18	-5.689871752289701
1115	19	-3.7499149031010823
1115	20	-4.128190137743559
1115	21	-3.7004444466130373
1115	22	-3.2558463469457024
1115	23	-3.8154387607803066
1115	24	-3.7305389259095136
1115	25	-3.725221563991184
1116	1	-1.435114629208158
1116	2	-1.321236356490953
1116	3	-1.7852251191292225
1116	4	-1.5586746723212137
1116	5	-1.4571394624184322
1116	6	-3.745144958947499
1116	7	-2.2816007198886723
1116	8	-2.767548403626744
1116	9	-3.67239812607054
1116	10	-3.0592722457720924
1116	11	-2.6170751551582967
1116	12	-2.5433601282080502
1116	13	-3.8069353439202303
1116	14	-6.883199385791524
1116	15	-4.848593457416804
1116	16	-3.6845803474703978
1116	17	-4.716898818559351
1116	18	-3.339000712729902
1116	19	-3.1720821090157436
1116	20	-3.8007814671261713
1116	21	-4.554195228546828
1116	22	-4.0178630925074295
1116	23	-3.1203401911505324
1116	24	-3.9453768111540555
1116	25	-3.9738353756437803
1201	1	0.7904751558955496
1201	2	0.8892991621456865
1201	3	1.1830271670592722
1201	4	1.160781727379387
1201	5	0.6691565189883235
1201	6	1.1248009508633174
1201	7	1.212926326561906
1201	8	1.2224042996623297
1201	9	1.7377114780907021
1201	10	1.4702265012989812
1201	11	1.7005918206726491
1201	12	1.483924704300847
1201	13	1.6999676978223732
1201	14	1.636924564677102
1201	15	1.630178889194319
1201	16	1.6408395328776066
1201	17	1.8235564800824946
1201	18	1.5945148648106695
1201	19	1.8417140079893741
1201	20	1.8404436912527231
1201	21	1.9078995357289656
1201	22	1.8787464736862702
1201	23	2.138917651859103
1201	24	2.0131607333222092
1201	25	2.0562385119072033
1202	1	1.047945519072659
1202	2	1.0326115082570801
1202	3	1.0233469104367536
1202	4	1.2213638242973701
1202	5	0.9819822056498921
1202	6	1.2119755295269243
1202	7	1.3728496210516283
1202	8	1.3904846189015885
1202	9	1.5828808351547181
1202	10	1.3377244317625596
1202	11	1.4492771343931494
1202	12	1.5825264405888362
1202	13	1.5218591479836903
1202	14	2.023459444325603
1202	15	1.67451048199446
1202	16	1.4193297529913735
1202	17	1.7635878149865576
1202	18	1.8417215212888465
1202	19	1.7922447910170547
1202	20	1.572908880588777
1202	21	1.8093475479127008
1202	22	1.731917242280261
1202	23	1.8183353161484561
1202	24	1.9072328380938188
1202	25	2.2225179364699486
1203	1	1.0131184948724616
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2211	17	0.32338960730218247
2211	18	-0.10934104589455274
2211	19	0.07203610020145135
2211	20	0.018151707488136992
2211	21	0.17720168248188628
2211	22	0.08562393119514411
2211	23	0.3475538137058791
2211	24	0.11494758010049821
2211	25	0.48535880300309486
2212	1	-0.003483295243768225
2212	2	0.08540299110187277
2212	3	-0.2457055154614629
2212	4	-0.057879329104657984
2212	5	-0.20375078864760354
2212	6	-0.27661933113802206
2212	7	-0.10335805969511114
2212	8	-0.11882674666421877
2212	9	-0.024064735049115882
2212	10	-0.3203871107894827
2212	11	-0.4193078348102972
2212	12	0.003566626475596024
2212	13	0.05482392216684673
2212	14	0.10798336556928945
2212	15	-0.32761881961023676
2212	16	-0.2695270662469653
2212	17	-0.2293571890764241
2212	18	0.15268693484501483
2212	19	-0.23838980525934517
2212	20	-0.031753049972039094
2212	21	0.10369432032727843
2212	22	0.13962840853170633
2212	23	0.13652933192946648
2212	24	0.1955592107453512
2212	25	0.3999383134695762
2213	1	-0.38876718294880774
2213	2	-0.31720707950428206
2213	3	-0.5463487723194866
2213	4	-0.32761224807151734
2213	5	-0.39287640101835564
2213	6	-0.09670716954416747
2213	7	-0.26044907006468065
2213	8	-0.25707131824013985
2213	9	-0.7487891272867202
2213	10	-0.3374670107250637
2213	11	-0.4362182578176643
2213	12	-0.4080488260932782
2213	13	-0.501990385009961
2213	14	-0.4935880229982459
2213	15	-0.5565988329165634
2213	16	-0.47903511224597395
2213	17	-0.5078808737919189
2213	18	-0.34593269697957396
2213	19	-0.4179589838275888
2213	20	-0.12041970220084863
2213	21	-0.4139217109310209
2213	22	-0.5677355528668357
2213	23	-0.34505654653769113
2213	24	-0.22729309099308637
2213	25	-0.01593030489306102
2214	1	-2.7368504130640687
2214	2	-1.4952870587646494
2214	3	-1.6304209207230684
2214	4	-3.539473341922463
2214	5	-1.9837593990472726
2214	6	-1.6043790842153989
2214	7	-1.3083235664907988
2214	8	-1.3378797523568338
2214	9	-1.1583191730044362
2214	10	-1.1689065839166233
2214	11	-1.3563629620104436
2214	12	-1.1415522806409797
2214	13	-1.4734965390017223
2214	14	-1.3578344830641669
2214	15	-1.7648459225784379
2214	16	-1.7122186250621638
2214	17	-3.7687876977066885
2214	18	-3.5786028627731774
2214	19	-2.3847976920448453
2214	20	-2.1526175681586395
2214	21	-2.1705746471936855
2214	22	-3.251420684937962
2214	23	-2.080621619188129
2214	24	-3.266251912126112
2214	25	-3.1895964256355747
2215	1	-2.069828698692845
2215	2	-3.755107678272669
2215	3	-2.263427159863806
2215	4	-3.736836567155027
2215	5	-2.4736878214300333
2215	6	-2.280758470526699
2215	7	-2.5243153654084054
2215	8	-1.9070828798248485
2215	9	-4.377640453420767
2215	10	-2.6775784076079
2215	11	-2.220661131419117
2215	12	-2.230072427952866
2215	13	-2.2913362832842417
2215	14	-1.9597764727722904
2215	15	-2.1684125413050026
2215	16	-2.240240721845744
2215	17	-2.5242537004168284
2215	18	-2.2774318288465487
2215	19	-2.3809527851955075
2215	20	-2.2154604922830394
2215	21	-2.8255191974019347
2215	22	-2.7673219726700076
2215	23	-2.656793380529564
2215	24	-2.6614807983916897
2215	25	-3.603565595717626
2216	1	-3.3162552517458472
2216	2	-3.3477843592141205
2216	3	-4.704810340461787
2216	4	-5.215172506976813
2216	5	-4.867161734621789
2216	6	-3.9254049381258547
2216	7	-5.516241280237132
2216	8	-3.314448889266668
2216	9	-4.1729411618009955
2216	10	-3.0081072080872993
2216	11	-3.378599289435652
2216	12	-3.744313111583633
2216	13	-3.246504925522995
2216	14	-4.2169309181748815
2216	15	-3.765692049433838
2216	16	-4.118340859721673
2216	17	-3.815185434779117
2216	18	-3.382982126765743
2216	19	-4.206692467642391
2216	20	-4.7597819405162305
2216	21	-4.4671634531398645
2216	22	-4.15070317282396
2216	23	-4.200180573170986
2216	24	-4.499704038037599
2216	25	-4.534338541341892
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	142294.0
3	1.0
4	1254.0
5	7505.0
6	8723.0
7	7618.0
8	6634.0
9	6412.0
10	5667.0
11	5427.0
12	5116.0
13	4938.0
14	5052.0
15	5137.0
16	5320.0
17	5863.0
18	6478.0
19	8026.0
20	10204.0
21	12970.0
22	16332.0
23	19306.0
24	22522.0
25	25520.0
26	28568.0
27	31361.0
28	35740.0
29	41903.0
30	50271.0
31	62424.0
32	77976.0
33	99091.0
34	129034.0
35	167948.0
36	226210.0
37	345658.0
38	904338.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.32920662769953	17.76172217677151	11.921145752220239	24.987925443308725
2	15.39122677398057	19.46607664979407	41.267331090640425	23.875365485584936
3	19.827131218440385	24.80361268558498	28.16164024917187	27.207615846802764
4	12.601983229948408	15.601901486164865	37.17569725875366	34.62041802513307
5	13.480864436001843	38.61638205967924	34.38337467080998	13.519378833508942
6	33.03484488825757	35.52606327168505	17.82145121227995	13.617640627777435
7	29.690129324159848	31.019590339129355	20.951103787701523	18.339176549009277
8	26.609815332159016	36.18869744385344	19.81003750315673	17.391449720830813
9	28.263590049341136	14.25579677713428	19.164740241301985	38.3158729322226
10	16.59917515620194	28.1331439166	32.94030147781236	22.327379449385692
11	35.77846201282829	21.522334278930614	23.9532914533239	18.74591225491719
12	24.843178192368086	23.83719009304682	30.663285053037004	20.656346661548096
13	30.14819094258029	21.33999126137113	25.075578127943437	23.436239668105145
14	21.83994751861862	21.614235102744576	26.573418872531285	29.972398506105524
15	23.803300531997525	30.784907741081163	22.43671148681254	22.975080240108767
16	22.862820872329888	27.07533393454596	27.405463378796597	22.656381814327556
17	22.465193264115825	27.98781744577009	27.04340595455182	22.503583335562265
18	22.296119376683535	26.392101238577148	29.39606776930242	21.9157116154369
19	24.579003800313245	25.795442298721454	26.54763843295881	23.07791546800649
20	25.016401386369463	26.226918096904267	27.05536510194032	21.70131541478595
21	24.22916619146237	25.727534503764797	25.962514836205923	24.080784468566907
22	24.626152067614974	27.40039736031912	26.002319967493708	21.9711306045722
23	23.269400049243995	27.00608312795336	27.02240601752174	22.702110805280906
24	24.261535417942458	25.813802659467566	27.52677540049533	22.397886522094645
25	23.510871057199054	26.546767315208154	27.30819951568822	22.634162111904576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	309.0
1	309.0
2	682.5
3	1056.0
4	1056.0
5	1056.0
6	1734.5
7	2413.0
8	2413.0
9	2413.0
10	2756.5
11	3100.0
12	3100.0
13	3100.0
14	4664.5
15	6229.0
16	6229.0
17	6229.0
18	12504.0
19	18779.0
20	18779.0
21	18779.0
22	32128.5
23	45478.0
24	45478.0
25	45478.0
26	71972.5
27	98467.0
28	98467.0
29	98467.0
30	132234.0
31	166001.0
32	166001.0
33	166001.0
34	191798.0
35	217595.0
36	217595.0
37	217595.0
38	239458.5
39	261322.0
40	261322.0
41	261322.0
42	280238.0
43	299154.0
44	299154.0
45	299154.0
46	319721.5
47	340289.0
48	340289.0
49	340289.0
50	334721.5
51	329154.0
52	329154.0
53	329154.0
54	297698.5
55	266243.0
56	266243.0
57	266243.0
58	234990.5
59	203738.0
60	203738.0
61	203738.0
62	171481.5
63	139225.0
64	139225.0
65	139225.0
66	109549.5
67	79874.0
68	79874.0
69	79874.0
70	59590.0
71	39306.0
72	39306.0
73	39306.0
74	28254.5
75	17203.0
76	17203.0
77	17203.0
78	11916.5
79	6630.0
80	6630.0
81	6630.0
82	4456.0
83	2282.0
84	2282.0
85	2282.0
86	1519.0
87	756.0
88	756.0
89	756.0
90	467.0
91	178.0
92	178.0
93	178.0
94	112.0
95	46.0
96	46.0
97	46.0
98	30.0
99	14.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33463780251889996
2	0.01104194721792049
3	0.009902386828882434
4	0.011985031677814055
5	0.013281772810167706
6	0.07972993204683514
7	0.07882614277277047
8	0.10432871837572563
9	0.08389522174469839
10	0.09033963222063776
11	0.08566350510699883
12	0.09686263306823491
13	0.08283425172731813
14	0.08743178846929926
15	0.08487760139042086
16	0.0865279991952346
17	0.09890598273133763
18	0.10094933239444036
19	0.09505505452010558
20	0.09324747597197625
21	0.08153751059496447
22	0.10075285646529586
23	0.09426915080352762
24	0.09045751777812445
25	0.0889643007166263
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2544841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.046572365320465
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.73029406052777	39.64185951763464
2	13.688168196319332	14.52220809549463
3	4.877915961043988	7.762701660584148
4	2.236590210949481	4.7457377790679605
5	1.2573134704743285	3.334808499870434
6	0.7494509540406753	2.385348256066629
7	0.4790657268369679	1.7788956322481446
8	0.3523072301587677	1.4950952783554148
9	0.2608518698538286	1.2453567831747245
>10	1.2451008552997285	11.707868058007536
>50	0.07317636101273302	2.6472629033965864
>100	0.04319968269641523	4.2672448404251035
>500	0.003804959773721508	1.4201625911437645
>1k	0.002536639849143313	2.380090237116467
>5k	2.2382116315970414E-4	0.6653598674138586
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTACAGTGGACATTTCTAAATT	6360	0.24991738187179474	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5391	0.21184034680359204	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5061	0.19887293548005555	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4772	0.18751662677550385	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4444	0.17462780582362514	No Hit
GTCCTACAGTGTGCATTTCTCATTT	4084	0.16048153892522166	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2964	0.11647093079685529	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2806	0.11026229143588932	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2697	0.10597911618053937	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	3.929518582889854E-5	0.0
13	0.0	0.0	0.0	3.929518582889854E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGGACC	1100	0.0	14.852378	4
CGATACT	60	2.5730671E-5	14.24792	4
GTCCTAT	250	0.0	12.57353	1
GGCGAGG	1365	0.0	12.038536	19
GGTATCA	1770	0.0	11.731851	1
ATTTTCG	65	8.036337E-4	11.6906	15
TAGAAAT	740	0.0	11.295649	4
GTAAACG	85	5.3336393E-5	11.174839	13
GTCCTAA	1435	0.0	11.01893	1
AGGACCT	2085	0.0	10.979213	5
TCGTCTC	140	8.378265E-9	10.855558	16
TGTAGGA	3250	0.0	10.755354	2
GTAGGAC	3190	0.0	10.749215	3
TGGCGAG	3145	0.0	10.721806	18
ACGATAC	90	9.533712E-5	10.554015	3
GGACCTG	2130	0.0	10.524285	6
GTCCTAC	3300	0.0	10.506807	1
TTAGGAC	1960	0.0	10.370935	3
GTATCAA	3650	0.0	10.3083105	1
GTATAAT	540	0.0	10.230987	1
>>END_MODULE