FastQCFastQC Report
Thu 26 May 2016
SRR1512873_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512873_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544841
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62120.24410169436911774No Hit
GTCCTACAGTGGACATTTCTAAATT60130.23628195238916697No Hit
GTCCTAAAGTGTGTATTTCTCATTT50080.19679029063112394No Hit
CTGTAGGACGTGGAATATGGCAAGA48730.19148544054422262No Hit
CTTTAGGACGTGAAATATGGCGAGG46500.18272261410437823No Hit
TATCAACGCAGAGTACTTTTTTTTT45160.17745705920330582No Hit
GTCCTACAGTGTGCATTTCTCATTT39200.1540371284492823No Hit
GGTATCAACGCAGAGTACTTTTTTT35710.1403231085949967No Hit
CTGTAGGACCTGGAATATGGCGAGA28840.1133273159305434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAGT400.005236392414.2679571
GTCCTAT2550.013.4286661
TAGGACC10500.013.3146634
AGGACCG1850.012.3379125
GGTATCA18250.012.1440521
GGACCGA752.0536793E-411.4125676
GGCGAGG13850.011.30768319
GTGCGCC2100.011.30152311
GAATACG1105.021866E-711.21866813
AGGACCT21950.011.1352695
GTCCTAA16300.011.029221
GTAAGAC2100.010.8714683
TCCAACG2550.010.79458118
TAATACT2650.010.7665744
TGGACCG1158.69868E-710.750975
GTCCTAC34200.010.7079211
TTATACT2950.010.6388354
TGGCGAG31300.010.58349418
ATTATAC2250.010.5694813
GGACCGT909.4138595E-510.5671936
Produced by FastQC (version 0.11.5)