##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512873_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2544841
Sequences flagged as poor quality	0
Sequence length	25
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.067810916281214	31.0	30.0	34.0	26.0	34.0
2	30.062333560328522	31.0	30.0	34.0	26.0	34.0
3	30.24941086692646	31.0	30.0	34.0	26.0	34.0
4	33.85463885562988	37.0	35.0	37.0	30.0	37.0
5	33.40679398044907	37.0	35.0	37.0	28.0	37.0
6	33.2719262225027	37.0	35.0	37.0	27.0	37.0
7	33.10563528330454	37.0	35.0	37.0	26.0	37.0
8	33.04512894911706	37.0	35.0	37.0	26.0	37.0
9	34.355292138094285	39.0	35.0	39.0	26.0	39.0
10	34.26159708995572	39.0	35.0	39.0	26.0	39.0
11	34.25139959628126	39.0	35.0	39.0	25.0	39.0
12	34.10067387314178	39.0	35.0	39.0	25.0	39.0
13	34.0992643548261	39.0	35.0	39.0	25.0	39.0
14	35.15455621785408	39.0	35.0	41.0	25.0	41.0
15	35.148206901727846	39.0	35.0	41.0	25.0	41.0
16	35.14055887970997	39.0	35.0	41.0	25.0	41.0
17	35.11580094787847	39.0	35.0	41.0	25.0	41.0
18	35.02638160890995	39.0	35.0	41.0	24.0	41.0
19	35.03562147890575	40.0	35.0	41.0	24.0	41.0
20	35.00502349655637	40.0	35.0	41.0	24.0	41.0
21	34.96098263113491	40.0	35.0	41.0	23.0	41.0
22	34.923145689652124	40.0	35.0	41.0	23.0	41.0
23	34.75815109863445	39.0	35.0	41.0	21.0	41.0
24	34.71291762432309	39.0	35.0	41.0	19.0	41.0
25	34.64331484756808	39.0	35.0	41.0	19.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.760931209588513
1101	2	0.5676185359844439
1101	3	0.5815022148886726
1101	4	0.5939891576941747
1101	5	0.6497446952974428
1101	6	0.6618116480134617
1101	7	0.7912169547676626
1101	8	0.5564823855238572
1101	9	0.9662677509888979
1101	10	0.6474914808215786
1101	11	0.9808303619501473
1101	12	0.9788458088187184
1101	13	1.061747229781048
1101	14	1.182065032953382
1101	15	0.8285370967455705
1101	16	1.109793787614386
1101	17	1.1524154835523959
1101	18	1.1063200519657173
1101	19	0.8192908115989894
1101	20	0.9198825035878357
1101	21	1.1100828132952714
1101	22	0.9560056132810857
1101	23	1.181523157990462
1101	24	1.1828890661883875
1101	25	1.2966782795869136
1102	1	0.8841188755409846
1102	2	0.9425659464251162
1102	3	0.6035807573870606
1102	4	0.7627773710249173
1102	5	0.9836547139690239
1102	6	0.904019422371853
1102	7	0.8821969262531866
1102	8	0.9916049125958892
1102	9	1.201363087887735
1102	10	1.1315580799551483
1102	11	1.1377167810950581
1102	12	1.166767752439462
1102	13	1.1398260192658682
1102	14	1.192692693998623
1102	15	1.3437495788766327
1102	16	1.3028095556128747
1102	17	1.3172065518513207
1102	18	0.7494354879272365
1102	19	1.0953502161694004
1102	20	1.2840258824624584
1102	21	1.1150364891209747
1102	22	0.943955749103317
1102	23	1.2030023792411555
1102	24	1.1643413193370264
1102	25	1.1996572284619376
1103	1	0.7232776753318539
1103	2	0.8630857375628089
1103	3	0.6935757713714743
1103	4	0.49327414380756096
1103	5	0.7862001361497164
1103	6	0.7900903486081887
1103	7	0.8065988183322119
1103	8	0.8982228443723912
1103	9	0.9202007866947355
1103	10	0.7966610266284846
1103	11	1.0465799998228889
1103	12	1.0443823988701268
1103	13	1.1061889782916694
1103	14	1.1393242895907605
1103	15	1.1701257066713566
1103	16	1.1218371364289226
1103	17	1.1370242983013057
1103	18	1.1376741525756202
1103	19	1.0711987176590796
1103	20	1.0319071549905345
1103	21	1.1560829046207814
1103	22	1.1274812794912137
1103	23	1.2407334301107582
1103	24	1.237372162458172
1103	25	1.0977491657442613
1104	1	0.6410666978734874
1104	2	0.7951988513012758
1104	3	0.6659141456341189
1104	4	0.6292333095277911
1104	5	0.7769191813280187
1104	6	0.5366233140632559
1104	7	0.5369838805220226
1104	8	0.5713154365899342
1104	9	0.6081163959191116
1104	10	0.7303350524183898
1104	11	0.735463470591263
1104	12	0.7661677093933505
1104	13	0.5864418764235637
1104	14	0.8923326447480378
1104	15	0.9543423061654508
1104	16	0.9250517350266847
1104	17	0.9215937680404096
1104	18	0.4856704260165685
1104	19	0.947282190296967
1104	20	0.8448386680431028
1104	21	0.9620186806782627
1104	22	0.8988249958822081
1104	23	0.847729972034287
1104	24	1.1053001520013765
1104	25	0.9036285968104494
1105	1	0.6796791865419678
1105	2	0.6153929535782439
1105	3	0.6352870459700668
1105	4	0.43862380704818804
1105	5	0.518648504636289
1105	6	0.7459444893418734
1105	7	0.7057995366837062
1105	8	0.6534942879737073
1105	9	0.8683014329798482
1105	10	0.5286473224551855
1105	11	0.7402307078333052
1105	12	0.8598860477925356
1105	13	0.8282298570133335
1105	14	0.9285428511150187
1105	15	0.9897926341129804
1105	16	0.8597581819951685
1105	17	0.6252571818941917
1105	18	0.6377954193509652
1105	19	0.9571659422299916
1105	20	0.9863253635051592
1105	21	0.7819488251884721
1105	22	0.7622601795861499
1105	23	0.9158950656193099
1105	24	0.9035430997963942
1105	25	0.6697326868227123
1106	1	-0.030075415791205984
1106	2	0.23830454911384535
1106	3	-1.5940371638350221
1106	4	-0.40946764460252183
1106	5	0.004822572900472721
1106	6	-0.08283248907539331
1106	7	0.07772575510746549
1106	8	0.13858381034077638
1106	9	0.3024849132632923
1106	10	-0.036885213921728166
1106	11	0.15246543133270052
1106	12	0.34592578742940105
1106	13	0.21774230720355803
1106	14	0.2693526093234908
1106	15	0.3163851946510121
1106	16	0.28982435462097556
1106	17	0.38952216088843983
1106	18	0.3822176259465948
1106	19	0.3261152940267493
1106	20	0.34885308568831164
1106	21	0.27461844888801323
1106	22	0.3000389791029292
1106	23	0.3139928280109032
1106	24	0.3177053038677258
1106	25	0.24895051556723757
1107	1	0.5433645284541555
1107	2	0.6467662838617549
1107	3	0.5392033627679496
1107	4	0.38841548447531693
1107	5	0.5193487341108565
1107	6	0.4919629948795148
1107	7	0.427419951803941
1107	8	0.4956132015481245
1107	9	0.5611264789116603
1107	10	0.5690894483784348
1107	11	0.5885215773033465
1107	12	0.8150007727796833
1107	13	0.6334999016046936
1107	14	0.7748930248811305
1107	15	0.6266003058930139
1107	16	0.8309075962570276
1107	17	0.8283007328717389
1107	18	0.8958489191455996
1107	19	0.8433394230370155
1107	20	0.8349905741273957
1107	21	0.8458959845490952
1107	22	0.8813219409571929
1107	23	1.036566346372716
1107	24	0.998897039109842
1107	25	0.992234845064246
1108	1	0.519354272570407
1108	2	0.46129020710070634
1108	3	0.47660250768717916
1108	4	0.3963058390654055
1108	5	0.5604597058243002
1108	6	0.5026194203751544
1108	7	0.396339195535063
1108	8	0.39290281907903335
1108	9	0.6354631652254028
1108	10	0.5473726373312289
1108	11	0.4651316303914683
1108	12	0.5148371017902775
1108	13	0.511486213499758
1108	14	0.6589949897725873
1108	15	0.7388686975298953
1108	16	0.6014171448508563
1108	17	0.6267725812456675
1108	18	0.6780495011455301
1108	19	0.7388942792054038
1108	20	0.6531581139218972
1108	21	0.8299200120164585
1108	22	0.8944728917625824
1108	23	0.9297157331522357
1108	24	0.8872109592519237
1108	25	0.9854808080424107
1109	1	0.20350309509923292
1109	2	0.17369322965474865
1109	3	0.3010854640166549
1109	4	0.2792057623042439
1109	5	-0.08548840127824064
1109	6	0.1604054024782826
1109	7	0.22763005115974266
1109	8	0.16582515192534686
1109	9	0.4453732515992499
1109	10	-0.08208941637496991
1109	11	0.31801567798495256
1109	12	0.4954198786906048
1109	13	0.3573792573722159
1109	14	0.3903105890765559
1109	15	0.4213560902146014
1109	16	0.4301252328083649
1109	17	0.5062591717843006
1109	18	0.6849255699666514
1109	19	0.6240885836385175
1109	20	0.7192538952834653
1109	21	0.7242811091728996
1109	22	0.6010145341996846
1109	23	0.5617543790980051
1109	24	0.5932734964281536
1109	25	0.6011036086084189
1110	1	0.4102958742466285
1110	2	0.3179486127992881
1110	3	0.3218245576324321
1110	4	0.2999888986092074
1110	5	0.4372771931458814
1110	6	0.46815512542112714
1110	7	0.42827726384833653
1110	8	0.49912661697138816
1110	9	0.44957395735648475
1110	10	0.6223468998658603
1110	11	0.5364566742090986
1110	12	0.6956177127212797
1110	13	0.7095847039847811
1110	14	0.7473874077621119
1110	15	0.7133295786641369
1110	16	0.6039855387081161
1110	17	0.6758050844974193
1110	18	0.744079377421329
1110	19	0.8839445676255977
1110	20	0.8755776914052049
1110	21	0.8831196354257429
1110	22	0.9427204543606749
1110	23	0.9396112904880525
1110	24	0.9768230149126538
1110	25	0.8531035226714607
1111	1	0.08212790092050426
1111	2	-0.10689053291577011
1111	3	-0.15142046354446137
1111	4	0.14457572387729556
1111	5	0.2756317033738753
1111	6	0.2148686515993674
1111	7	0.1370814451586
1111	8	0.13763612404537184
1111	9	0.11200884966972069
1111	10	0.06125291049936976
1111	11	0.04708116731534062
1111	12	0.27480668271316944
1111	13	0.2321025015877325
1111	14	0.1039071878828679
1111	15	0.00568607140992583
1111	16	0.023927865463100773
1111	17	0.1599179077829902
1111	18	0.022341970915064735
1111	19	-0.15690070587174176
1111	20	0.045439816303819214
1111	21	0.15982685359281845
1111	22	0.004320886910015531
1111	23	0.12644737231197212
1111	24	0.201096241910939
1111	25	0.09882578995090796
1112	1	-0.031082670388101974
1112	2	-0.46184816733021705
1112	3	-0.11527997593998052
1112	4	0.02366666866761591
1112	5	-0.1305487483444665
1112	6	0.01964507307426544
1112	7	0.036918930470349665
1112	8	0.03561695456188119
1112	9	0.11185195351902877
1112	10	-0.2259725892030886
1112	11	-0.05507471991517576
1112	12	0.31775478373810273
1112	13	0.04475937508303929
1112	14	-0.1684317851330519
1112	15	-0.27738485205411934
1112	16	-0.37899860721298495
1112	17	-0.2655573268513649
1112	18	5.028362906713824E-4
1112	19	-0.007588493406444741
1112	20	-0.07899103490461812
1112	21	-0.02859932154243694
1112	22	0.13726853482707924
1112	23	0.28714322516670165
1112	24	0.2147034940533814
1112	25	0.1321754955818264
1113	1	-0.96256785645528
1113	2	-0.7206775493308477
1113	3	-0.7054802397274749
1113	4	-2.288970977984178
1113	5	-0.9921001561768463
1113	6	-0.586331821330873
1113	7	-0.7000188325504411
1113	8	-0.6013663230936785
1113	9	-0.6243704843785025
1113	10	-0.6236925100511002
1113	11	-0.8829361551295989
1113	12	-0.5082866110260014
1113	13	-0.674088485241711
1113	14	-0.7257279409356343
1113	15	-0.8422630061011418
1113	16	-0.9917503220042505
1113	17	-1.1683039294960125
1113	18	-0.8802690439602401
1113	19	-0.7768044700665229
1113	20	-0.5344611982965262
1113	21	-0.679757407728637
1113	22	-0.6991122567013051
1113	23	-0.47165100817690586
1113	24	-0.5102711314014954
1113	25	-0.7344487020615205
1114	1	-1.0533340241879117
1114	2	-2.184336452710369
1114	3	-1.528204455675926
1114	4	-0.9292027692217673
1114	5	-0.885614999442069
1114	6	-0.4173324303240804
1114	7	-0.48330968230271765
1114	8	-0.5425051519089763
1114	9	-0.7610564936525606
1114	10	-0.8833656380370982
1114	11	-0.8612880917523142
1114	12	-0.9362733885634853
1114	13	-1.0591509073652787
1114	14	-1.051815701759665
1114	15	-1.1944187998003102
1114	16	-1.586361903526445
1114	17	-1.6591996923140897
1114	18	-1.2657538054959403
1114	19	-1.7329691212434852
1114	20	-0.9740440027454369
1114	21	-1.3845727555351957
1114	22	-1.0654466975394143
1114	23	-0.8879649465424961
1114	24	-0.9450479847437165
1114	25	-1.5879951817364244
1115	1	-1.1673622087678694
1115	2	-1.2413446152508918
1115	3	-0.811459933001526
1115	4	-0.5540455988084645
1115	5	-1.0041305619893848
1115	6	-0.8713152026390034
1115	7	-0.8647038593528507
1115	8	-0.9531151724804019
1115	9	-2.0079039503669946
1115	10	-1.6531892392091976
1115	11	-1.989184838467395
1115	12	-2.521261057889884
1115	13	-2.5192885673002365
1115	14	-1.9218489275882433
1115	15	-2.3092095181077212
1115	16	-1.9302875643409294
1115	17	-1.9678575752589111
1115	18	-2.329599834583121
1115	19	-2.7583179347487032
1115	20	-2.9089509313211224
1115	21	-2.2076907011366345
1115	22	-2.2534128319389453
1115	23	-2.2154425721554176
1115	24	-2.701272843407338
1115	25	-2.759605528604329
1116	1	-1.5642793168500724
1116	2	-1.6414586912850204
1116	3	-1.6192011662292671
1116	4	-1.3514208176612073
1116	5	-1.8208139238901175
1116	6	-2.670580225204187
1116	7	-2.025143219843123
1116	8	-2.3158209553546065
1116	9	-3.2681918439510866
1116	10	-2.095004687026993
1116	11	-3.0984480640769476
1116	12	-5.184776180545818
1116	13	-4.189252341061909
1116	14	-3.443927898787596
1116	15	-4.569844156379265
1116	16	-4.067926429168253
1116	17	-3.8979660023355827
1116	18	-3.803029958334328
1116	19	-4.035563734082633
1116	20	-5.817773467273948
1116	21	-4.527026685705398
1116	22	-3.9837848340152853
1116	23	-4.616656307606775
1116	24	-4.640809254664347
1116	25	-3.8208653912936903
1201	1	0.6566861570933433
1201	2	0.8893826152323783
1201	3	0.9077905704061564
1201	4	0.7547011096797647
1201	5	0.18819150275658814
1201	6	0.8553566917228252
1201	7	1.0636825254055111
1201	8	0.9874953061441332
1201	9	0.9203749762129334
1201	10	0.9417640987709746
1201	11	0.7646158895375663
1201	12	1.2170289049353968
1201	13	1.2000500324519336
1201	14	1.30912516627793
1201	15	1.1870788183235064
1201	16	1.2029305660803828
1201	17	1.0937624293879296
1201	18	1.3819272332607397
1201	19	1.4139171953628207
1201	20	1.448686344081601
1201	21	1.1707469924817104
1201	22	1.1115141739470502
1201	23	1.291196600707032
1201	24	1.0425468591948928
1201	25	1.3366421479755957
1202	1	0.8908580506661075
1202	2	1.0086680414507505
1202	3	0.9431771226646042
1202	4	0.8619777854736199
1202	5	0.894323300387768
1202	6	0.9201457847352259
1202	7	0.9772196815440779
1202	8	0.8498877167826109
1202	9	1.1392941210168033
1202	10	1.2049617713384748
1202	11	1.0798314927808974
1202	12	1.1417190051221837
1202	13	1.114798928699173
1202	14	1.225323136009628
1202	15	1.2272371113274119
1202	16	1.2900146688567418
1202	17	1.1368887825463574
1202	18	1.2224342962171022
1202	19	1.3448297017306245
1202	20	1.1833772137114167
1202	21	1.3753348628756612
1202	22	1.2616058437976179
1202	23	1.4597091346686568
1202	24	1.4235219823057719
1202	25	1.4528328212647779
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2211	8	-0.27071158439365917
2211	9	0.04922177035296471
2211	10	0.04061025871516222
2211	11	0.12688837178008328
2211	12	-0.011535275683577595
2211	13	0.12174904310641921
2211	14	0.12073451749558473
2211	15	0.12447179777555561
2211	16	0.13141983231579246
2211	17	-0.08187137007934808
2211	18	-0.09902112734301483
2211	19	-0.009608299309931567
2211	20	0.015110034467191724
2211	21	-0.03883372479378977
2211	22	-0.11842898625081943
2211	23	-0.14701449137820077
2211	24	0.11874548933187867
2211	25	-0.1718862005784345
2212	1	-0.21017626384575294
2212	2	-0.2407110536123902
2212	3	-0.1490983961474157
2212	4	-0.22906136466470883
2212	5	-0.4144695380199863
2212	6	-0.24044476854776065
2212	7	-0.4140698150066555
2212	8	-0.3066109260540699
2212	9	-0.35422585785341454
2212	10	-0.5545460215482052
2212	11	-0.2708155683245437
2212	12	-0.09393699939568023
2212	13	-0.11061795364172866
2212	14	-0.0709397709969295
2212	15	-0.2596211743463428
2212	16	-0.2555461633114291
2212	17	-0.405312204628153
2212	18	-0.40707996337576446
2212	19	-0.1755321009427817
2212	20	-0.14182052269816126
2212	21	-0.1777498686433674
2212	22	-0.11669518822518654
2212	23	-0.5481499426036791
2212	24	-0.5204453080162992
2212	25	-0.45001724089932793
2213	1	-0.5135830271083925
2213	2	-0.10864886439354038
2213	3	-0.21736964585970853
2213	4	-0.5056638911552369
2213	5	-0.22855605485864317
2213	6	-0.21924302855105537
2213	7	-0.6781292230914815
2213	8	-0.5981108019052996
2213	9	-0.4366750613622159
2213	10	-0.3582844357981685
2213	11	-0.281815514938053
2213	12	-0.5323865927265956
2213	13	-0.40721032158948134
2213	14	-0.40191618726248635
2213	15	-0.619497876733277
2213	16	-1.1314835747011784
2213	17	-0.8077390109928331
2213	18	-0.5258701323037229
2213	19	-0.5859199789858707
2213	20	-0.41297813070129763
2213	21	-0.41131765765189243
2213	22	-0.5892900386447977
2213	23	-0.68915890196498
2213	24	-0.4745289591083832
2213	25	-0.5523712955781761
2214	1	-1.0714425860620267
2214	2	-0.4219217139941769
2214	3	-0.5811248770613311
2214	4	-0.6355458316275602
2214	5	-0.9532436230134351
2214	6	-1.4901071387015392
2214	7	-0.8855121614528016
2214	8	-0.7627907778736613
2214	9	-0.5542063251217684
2214	10	-0.8567006727896
2214	11	-0.9123872333234928
2214	12	-0.6583988842500972
2214	13	-0.9989703303132984
2214	14	-0.6987552452552706
2214	15	-0.8327117788701983
2214	16	-0.9778333584576089
2214	17	-1.0555750321110438
2214	18	-1.0065614944942212
2214	19	-1.092640812850732
2214	20	-1.2529151916815735
2214	21	-1.4461234087845511
2214	22	-1.8080021034314555
2214	23	-1.8023683804702415
2214	24	-1.459035960791418
2214	25	-2.1269722428526023
2215	1	-1.4036830652413386
2215	2	-0.856235637129295
2215	3	-0.9860933280418855
2215	4	-1.0311805114403398
2215	5	-1.5618243120207396
2215	6	-1.3212258403284558
2215	7	-1.7005191446349741
2215	8	-1.521165920239131
2215	9	-1.6307225693336278
2215	10	-1.4376988313909678
2215	11	-1.3627141543220844
2215	12	-1.4900392462117011
2215	13	-1.6070771605305225
2215	14	-1.833436616785498
2215	15	-1.6963241000300755
2215	16	-1.7982112608426988
2215	17	-1.8428867355918257
2215	18	-1.5922894081022818
2215	19	-1.9180919139489205
2215	20	-1.8572041333138145
2215	21	-1.6239529484764574
2215	22	-1.814060429629201
2215	23	-2.1485954876870323
2215	24	-2.1751488223165794
2215	25	-2.533925497098629
2216	1	-1.5674874665528655
2216	2	-0.9719553811575139
2216	3	-1.009708113408525
2216	4	-1.3450610368304652
2216	5	-2.2372719825021363
2216	6	-2.389400190046125
2216	7	-2.3699159252563433
2216	8	-2.3910593343492472
2216	9	-4.246129047553612
2216	10	-2.304470671084623
2216	11	-2.398085298789752
2216	12	-2.896569169673512
2216	13	-2.5787180180895852
2216	14	-2.8449540680782732
2216	15	-2.7278335613395512
2216	16	-2.0841792789858005
2216	17	-2.387851401839505
2216	18	-2.779439907232643
2216	19	-3.07867456155655
2216	20	-2.3478774721037183
2216	21	-2.8657968928973574
2216	22	-2.373371317930207
2216	23	-3.6469144853338804
2216	24	-2.8582503730554087
2216	25	-2.981946149256654
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	79471.0
3	1.0
4	1311.0
5	11529.0
6	13238.0
7	10173.0
8	8219.0
9	7013.0
10	6131.0
11	5535.0
12	4929.0
13	4647.0
14	4550.0
15	4592.0
16	4647.0
17	4965.0
18	5361.0
19	6492.0
20	8412.0
21	10925.0
22	13695.0
23	16415.0
24	19408.0
25	21917.0
26	24661.0
27	27117.0
28	30824.0
29	36771.0
30	46292.0
31	58657.0
32	74727.0
33	95750.0
34	123456.0
35	161499.0
36	220310.0
37	341214.0
38	1029987.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.99896289589984	18.690487942337484	12.954755596212939	27.355793565549735
2	13.828594925597152	19.96326736475971	39.93160590882806	26.276531800815086
3	17.757944540125752	24.702298368998314	29.043526654996256	28.496230435879678
4	11.762380408581581	16.21142088712802	36.39241625555172	35.63378244873868
5	13.177922269838923	38.49756641272257	34.671622926027126	13.652888391411386
6	33.031888771675014	36.00545588444031	17.46354847169573	13.499106872188948
7	29.25379238475378	30.934416075975502	21.135228132957128	18.676563406313594
8	26.449768372814596	36.47187547055722	19.55796026541098	17.5203958912172
9	27.60557545629554	14.092828894773954	19.06524953484149	39.23634611408902
10	16.00187447024986	28.48954967110281	33.09922111987017	22.40935473877716
11	35.478207083498546	21.502312843581354	23.97108817807029	19.04839189484981
12	24.404343223419332	24.05131485776305	30.74148531262108	20.802856606196542
13	29.880877949904527	21.293385745515877	25.28450297304084	23.541233331538756
14	21.58839998821137	21.478647844154313	26.68454608863085	30.24840607900347
15	23.72945235493	30.75582636048627	22.390767899397645	23.123953385186084
16	22.8603731943319	27.01776213871901	27.275128054181636	22.846736612767458
17	22.23057936621149	28.104525079094866	27.073098942952274	22.59179661174137
18	22.130183467527225	26.351661747293694	29.5393839983558	21.978770786823283
19	24.39127901059532	25.75303471427847	26.77936170304735	23.07632457207886
20	24.782686090691644	26.07629377468985	27.244391648764292	21.89662848585421
21	23.862508856180348	25.625021366824463	26.307384413829904	24.205085363165285
22	24.378407393173777	27.228298584504923	26.193339133318716	22.199954889002584
23	22.96725021327462	26.831185131016046	27.255612433153974	22.94595222255536
24	24.127558460430336	25.80243716601548	27.59260794682261	22.477396426731573
25	23.26443970369858	26.458234522313965	27.523527010135407	22.753798763852043
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	91.0
1	91.0
2	269.0
3	447.0
4	447.0
5	447.0
6	1177.0
7	1907.0
8	1907.0
9	1907.0
10	2409.5
11	2912.0
12	2912.0
13	2912.0
14	4580.5
15	6249.0
16	6249.0
17	6249.0
18	12755.5
19	19262.0
20	19262.0
21	19262.0
22	33249.5
23	47237.0
24	47237.0
25	47237.0
26	74075.0
27	100913.0
28	100913.0
29	100913.0
30	134342.0
31	167771.0
32	167771.0
33	167771.0
34	193346.5
35	218922.0
36	218922.0
37	218922.0
38	240339.5
39	261757.0
40	261757.0
41	261757.0
42	281255.0
43	300753.0
44	300753.0
45	300753.0
46	320554.5
47	340356.0
48	340356.0
49	340356.0
50	335138.5
51	329921.0
52	329921.0
53	329921.0
54	297933.5
55	265946.0
56	265946.0
57	265946.0
58	233590.0
59	201234.0
60	201234.0
61	201234.0
62	169412.5
63	137591.0
64	137591.0
65	137591.0
66	107806.5
67	78022.0
68	78022.0
69	78022.0
70	58198.5
71	38375.0
72	38375.0
73	38375.0
74	27295.0
75	16215.0
76	16215.0
77	16215.0
78	11144.0
79	6073.0
80	6073.0
81	6073.0
82	4101.5
83	2130.0
84	2130.0
85	2130.0
86	1384.5
87	639.0
88	639.0
89	639.0
90	373.5
91	108.0
92	108.0
93	108.0
94	58.5
95	9.0
96	9.0
97	9.0
98	5.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08582068585031442
2	0.04141712586365907
3	0.02023702070188275
4	0.005304850086901304
5	0.017643538437175445
6	0.060200224689872565
7	0.07528957604816962
8	0.05163387417917269
9	0.021494466648407505
10	0.04672197595056037
11	0.005422735644387999
12	1.1788555748669563E-4
13	0.005501326016045796
14	6.287229732623767E-4
15	0.012888820951878724
16	0.008448464953213186
17	0.004401060812836637
18	0.005501326016045796
19	0.0014932170614981447
20	7.859037165779709E-4
21	3.1436148663118834E-4
22	2.750663008022898E-4
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2544841.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.62517538044625
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.48652945734952	37.937549713359985
2	14.324519367059722	14.790116491301141
3	5.0738232278586395	7.8581104196275255
4	2.3925892886981486	4.940713665696763
5	1.3125837838227938	3.3881184020690744
6	0.8064499691230999	2.49798726549213
7	0.5215434399808511	1.8847340090272877
8	0.35379050300249487	1.4611597412352078
9	0.26682251104641785	1.2397283035400144
>10	1.33081318207963	12.191964986943473
>50	0.07852918747840985	2.7766661935195986
>100	0.04505679186008377	4.354429437451015
>500	0.003894657391748256	1.3445947493661627
>1k	0.002825535754791909	2.6547329277157545
>5k	2.2909749363177642E-4	0.6793936936547803
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	6212	0.24410169436911774	No Hit
GTCCTACAGTGGACATTTCTAAATT	6013	0.23628195238916697	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	5008	0.19679029063112394	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4873	0.19148544054422262	No Hit
CTTTAGGACGTGAAATATGGCGAGG	4650	0.18272261410437823	No Hit
TATCAACGCAGAGTACTTTTTTTTT	4516	0.17745705920330582	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3920	0.1540371284492823	No Hit
GGTATCAACGCAGAGTACTTTTTTT	3571	0.1403231085949967	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2884	0.1133273159305434	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.929518582889854E-5	0.0
11	0.0	0.0	0.0	3.929518582889854E-5	0.0
12	0.0	0.0	0.0	7.859037165779709E-5	0.0
13	0.0	0.0	0.0	7.859037165779709E-5	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTAGT	40	0.0052363924	14.267957	1
GTCCTAT	255	0.0	13.428666	1
TAGGACC	1050	0.0	13.314663	4
AGGACCG	185	0.0	12.337912	5
GGTATCA	1825	0.0	12.144052	1
GGACCGA	75	2.0536793E-4	11.412567	6
GGCGAGG	1385	0.0	11.307683	19
GTGCGCC	210	0.0	11.301523	11
GAATACG	110	5.021866E-7	11.218668	13
AGGACCT	2195	0.0	11.135269	5
GTCCTAA	1630	0.0	11.02922	1
GTAAGAC	210	0.0	10.871468	3
TCCAACG	255	0.0	10.794581	18
TAATACT	265	0.0	10.766574	4
TGGACCG	115	8.69868E-7	10.75097	5
GTCCTAC	3420	0.0	10.707921	1
TTATACT	295	0.0	10.638835	4
TGGCGAG	3130	0.0	10.583494	18
ATTATAC	225	0.0	10.569481	3
GGACCGT	90	9.4138595E-5	10.567193	6
>>END_MODULE