FastQCFastQC Report
Thu 26 May 2016
SRR1512872_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512872_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876966
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT106100.5652739580791554No Hit
TATCAACGCAGAGTACTTTTTTTTT61450.32739005394876625No Hit
GGTATCAACGCAGAGTACTTTTTTT58460.31146009037990036No Hit
GTCCTACAGTGGACATTTCTAAATT47770.2545064748109449No Hit
CTGTAGGACGTGGAATATGGCAAGA40970.21827779512255419No Hit
GTCCTAAAGTGTGTATTTCTCATTT40590.2162532512576147No Hit
CTTTAGGACGTGAAATATGGCGAGG36730.19568814778743995No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29540.15738164676397973No Hit
GTACTGGTTCACTATCGGTCAGTCA24390.12994374964703675No Hit
GTACTTTTTTTTTTTTTTTTTTTTT23870.12717332120027747No Hit
GTCCTACAGTGTGCATTTCTCATTT23080.12296440106000855No Hit
GAGTACTTTTTTTTTTTTTTTTTTT19670.10479678374568319No Hit
CTGAAGGACCTGGAATATGGCGAGA18820.10026819878463435No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTA350.002171869616.28409214
GGTCTAC1800.012.1633041
GGTATCA19600.012.0931521
TAGGACC6150.011.8928074
CCCGTTT1302.6193447E-911.69114212
TCCAACG1950.011.69114218
TATGACT1157.088238E-811.5637564
GTCCTAT2150.011.5114781
AATGTCC5750.011.3991688
TAGAAAT5900.011.2697624
CCTAATA1354.7384674E-911.2578362
GTCCTAA12100.011.1711851
GTCCTAC21900.011.1273581
AATAACG600.005879618711.0825247
CCGTTTC1408.365532E-910.85606113
GTATCAA43150.010.8317081
TGTAGGA23050.010.7969012
TCCTACA23250.010.7857342
AAATGTC6200.010.7250247
GGACGTG24200.010.7161616
Produced by FastQC (version 0.11.5)