##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512872_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1876966
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.546957163848464	31.0	30.0	34.0	16.0	34.0
2	28.60705095350688	31.0	29.0	34.0	16.0	34.0
3	28.699387202538563	31.0	29.0	34.0	16.0	34.0
4	32.02849705322313	35.0	33.0	37.0	19.0	37.0
5	32.20653490793121	35.0	33.0	37.0	22.0	37.0
6	32.080041940024486	36.0	33.0	37.0	19.0	37.0
7	31.982931496894455	35.0	33.0	37.0	17.0	37.0
8	31.89146260507649	35.0	33.0	37.0	17.0	37.0
9	33.107233162454726	38.0	33.0	39.0	17.0	39.0
10	32.94986430228358	38.0	33.0	39.0	15.0	39.0
11	33.01996786303002	38.0	33.0	39.0	15.0	39.0
12	32.90973837565518	38.0	33.0	39.0	12.0	39.0
13	32.87703027119298	38.0	33.0	39.0	11.0	39.0
14	33.706448598429596	39.0	33.0	41.0	10.0	41.0
15	33.890439677649994	39.0	33.0	41.0	10.0	41.0
16	33.85250025839573	39.0	33.0	41.0	10.0	41.0
17	33.63151596779057	39.0	33.0	41.0	10.0	41.0
18	33.63851236516804	39.0	33.0	41.0	10.0	41.0
19	33.75871486217651	39.0	33.0	41.0	8.0	41.0
20	33.721997095312325	39.0	33.0	41.0	2.0	41.0
21	33.60837276754081	39.0	33.0	41.0	2.0	41.0
22	33.627643761261524	39.0	33.0	41.0	2.0	41.0
23	33.30025530563686	39.0	33.0	41.0	2.0	41.0
24	33.370244319822525	39.0	33.0	41.0	2.0	41.0
25	33.09048538971937	39.0	33.0	41.0	2.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9012544625751424
1101	2	0.6096736270361625
1101	3	0.9303846711275021
1101	4	1.1693511255032405
1101	5	1.0052747110113387
1101	6	1.1313775212817063
1101	7	0.7775287452217476
1101	8	0.8592341163843784
1101	9	1.1335613803126776
1101	10	1.0452422150713119
1101	11	1.1565916896494812
1101	12	0.8129815356199188
1101	13	1.1707309162255868
1101	14	1.4292029328636886
1101	15	1.2868276767252311
1101	16	1.2939802993523486
1101	17	0.5740587163755464
1101	18	1.1189787773858697
1101	19	0.5919522434426767
1101	20	-0.0710147097542091
1101	21	1.0225589579655434
1101	22	1.0284559392436918
1101	23	0.6904155943618449
1101	24	1.1741672137320904
1101	25	0.9516904586970938
1102	1	1.2759251896017894
1102	2	1.2586947722192825
1102	3	1.257912043205959
1102	4	1.3265147064983296
1102	5	1.2562458642747814
1102	6	1.0988807534090483
1102	7	1.0918493419532602
1102	8	1.1707553878224743
1102	9	1.5666470013861442
1102	10	1.4787518003489737
1102	11	1.3939602582210853
1102	12	1.4111837910387806
1102	13	1.4216479061049867
1102	14	1.7301105798749816
1102	15	1.5390745102504653
1102	16	1.6504982124081167
1102	17	1.7745148755982214
1102	18	1.7971417170056867
1102	19	1.4286901684169209
1102	20	1.6661542497027924
1102	21	1.7295176271371133
1102	22	1.6985665891033008
1102	23	1.5042239746620254
1102	24	1.049361772647714
1102	25	1.2589234799281286
1103	1	0.778376599859282
1103	2	0.9294721719126606
1103	3	0.9874747988501866
1103	4	1.0445885346812815
1103	5	0.8838129213159363
1103	6	0.5803579684294391
1103	7	0.4598732927489415
1103	8	0.680544141708026
1103	9	0.6980927942556718
1103	10	0.9664677296304376
1103	11	0.7447740376484404
1103	12	0.6636521487519218
1103	13	0.8581621341272765
1103	14	0.8307111591040339
1103	15	0.8821138102542108
1103	16	0.8682129475272262
1103	17	0.5615904202387796
1103	18	0.9685436997976922
1103	19	0.9049551663120567
1103	20	1.0319868522650566
1103	21	0.9818547476136175
1103	22	0.8159005191157362
1103	23	0.3145086074812298
1103	24	0.401136551215032
1103	25	0.79817004791758
1104	1	0.962846084119743
1104	2	0.8632685496161976
1104	3	0.8415238845786277
1104	4	1.1310300226249268
1104	5	0.8015960751226743
1104	6	0.6821814065291605
1104	7	0.9194079605727516
1104	8	0.7039726458799578
1104	9	1.0580828489172163
1104	10	1.0141554857087272
1104	11	0.7623227858782258
1104	12	1.1043899378237754
1104	13	1.1369338942820093
1104	14	1.4355586134944502
1104	15	1.123153315558703
1104	16	0.9371983339300982
1104	17	1.1295262047388022
1104	18	0.9413878207980488
1104	19	0.6163240967722103
1104	20	0.6500002992702036
1104	21	0.7484186520854266
1104	22	0.6721400372773232
1104	23	0.958514886657845
1104	24	1.0309076193557303
1104	25	0.6625322227324304
1105	1	0.7027573302378194
1105	2	0.7321430327337239
1105	3	0.8219283115249603
1105	4	1.0402008328364332
1105	5	0.7664296828067592
1105	6	0.9604550897869757
1105	7	1.0072412118836915
1105	8	1.0348120743253233
1105	9	0.7197461498375617
1105	10	0.8899488401981799
1105	11	0.9037852450791632
1105	12	1.0850340681318968
1105	13	1.0515082829323745
1105	14	1.1139481180226483
1105	15	1.1143578284871296
1105	16	1.1379368182694805
1105	17	1.4134629683050193
1105	18	1.2067670187887813
1105	19	0.9657175553452504
1105	20	1.1701905346461743
1105	21	0.8908618445099492
1105	22	0.9984413649086861
1105	23	1.2577134713473725
1105	24	1.4670899579554657
1105	25	1.6591261723084543
1106	1	1.0011719346349608
1106	2	0.9719591041073166
1106	3	0.9373642479480857
1106	4	1.0779665950176138
1106	5	1.0325954364617616
1106	6	1.0398972882148456
1106	7	0.8795178674426403
1106	8	0.8592594713539512
1106	9	1.0639013357674756
1106	10	0.7390506309325886
1106	11	0.6960223960091056
1106	12	0.8460625621481768
1106	13	0.9492097671980204
1106	14	1.3075778781765024
1106	15	1.0980823002785058
1106	16	1.1580401271374114
1106	17	1.248263514927828
1106	18	1.257725148145127
1106	19	1.211653054801701
1106	20	1.3934077935270182
1106	21	1.325676127754356
1106	22	1.2193077667901306
1106	23	1.5376456882249485
1106	24	1.2298052143684544
1106	25	1.3655951737271224
1107	1	1.0052500610894022
1107	2	0.973735405305117
1107	3	0.9674024354934083
1107	4	1.103720721467461
1107	5	0.9063801405283982
1107	6	1.0911703021579555
1107	7	0.800776596968408
1107	8	1.042698625826766
1107	9	1.0971004821499974
1107	10	1.2228586750246677
1107	11	1.1638284451093526
1107	12	1.208569241808334
1107	13	0.9802923375657286
1107	14	1.3806523835368907
1107	15	1.2154271224357842
1107	16	1.274677393722797
1107	17	1.5392823742659658
1107	18	1.4223625388429042
1107	19	1.329694745074626
1107	20	1.3797792182568713
1107	21	1.3736895824573523
1107	22	1.358962711347516
1107	23	1.5917971903871475
1107	24	1.4568671177239736
1107	25	1.6652091611940634
1108	1	0.9132668754496258
1108	2	0.8957966275988198
1108	3	0.8936649253560311
1108	4	1.0332225270901603
1108	5	0.8335111297650002
1108	6	0.9940816275202948
1108	7	0.9467366924469367
1108	8	0.9407093939803488
1108	9	1.1819892455508878
1108	10	1.09353444008768
1108	11	0.8770224801233795
1108	12	0.8546169835053661
1108	13	1.0409049232530379
1108	14	0.9859741070917565
1108	15	0.9766823622695711
1108	16	0.7805443818215991
1108	17	1.0586463270407407
1108	18	1.2010978077807621
1108	19	1.2150927523590482
1108	20	1.2735358546239368
1108	21	1.1069616242647626
1108	22	1.0136280946744733
1108	23	1.5066069461135427
1108	24	1.3945061420451594
1108	25	1.537078131369249
1109	1	0.7682249661529639
1109	2	0.7804792451924065
1109	3	0.8934942612974055
1109	4	0.8442812027703752
1109	5	0.7905953926178526
1109	6	0.8801546519727736
1109	7	0.7425829491134657
1109	8	0.704782488830368
1109	9	0.9296718175082148
1109	10	0.9195772424559223
1109	11	0.8035692022686334
1109	12	0.7656289458677747
1109	13	0.8190817314398515
1109	14	1.0512664894898407
1109	15	0.9712037906022672
1109	16	0.5286546687781666
1109	17	1.027936900866159
1109	18	1.0595125951718742
1109	19	0.9886728457124008
1109	20	0.9074306915864625
1109	21	0.9565585095402085
1109	22	0.8377690531935542
1109	23	1.2352651946117348
1109	24	0.9854082931340429
1109	25	0.6386448984912008
1110	1	0.5844410222618208
1110	2	0.5966348458384303
1110	3	0.6113617683428956
1110	4	0.5777112943167424
1110	5	0.5003871771514881
1110	6	0.578713546258939
1110	7	0.341135024200252
1110	8	0.4541024201343902
1110	9	0.6428609338007618
1110	10	0.5512546008240804
1110	11	0.47629405155272053
1110	12	0.5427386066617927
1110	13	0.5406951562219291
1110	14	0.5146975105251386
1110	15	0.6119179696629473
1110	16	0.7248467924813227
1110	17	0.811328293179784
1110	18	0.12126657945765373
1110	19	0.578737614843817
1110	20	0.5522308641774174
1110	21	0.6199567497888907
1110	22	0.7623534605692086
1110	23	0.7988305770842743
1110	24	0.6752802789677901
1110	25	0.907613242043567
1111	1	0.4344508829688323
1111	2	0.4155901915148732
1111	3	0.5156110836867782
1111	4	0.39044931961494456
1111	5	0.4882646738830516
1111	6	0.2990435956366895
1111	7	0.3738638403511665
1111	8	0.6030836786792904
1111	9	0.5421965939044284
1111	10	0.5691452084215314
1111	11	0.19129124286102694
1111	12	0.02433662836494932
1111	13	0.34913867172386404
1111	14	0.46773216902762726
1111	15	0.4586240842508005
1111	16	0.5764109014286518
1111	17	0.09365763337243038
1111	18	0.4609659168421558
1111	19	0.49934795495472173
1111	20	0.4966408146403225
1111	21	0.5801262600899193
1111	22	0.2114913662947373
1111	23	0.5551444036072013
1111	24	0.6014596539048327
1111	25	0.7161728814221817
1112	1	0.36320892195819354
1112	2	0.23714340671383738
1112	3	0.2491706499604689
1112	4	0.4579923209586667
1112	5	0.4159072743929073
1112	6	0.13598836170170614
1112	7	0.13877069563973876
1112	8	0.2964509342712418
1112	9	0.13099874820845514
1112	10	0.2804062235259863
1112	11	0.23584105782607878
1112	12	0.2744546565619217
1112	13	0.2718756692452473
1112	14	0.18693909346447413
1112	15	-0.1055901577711893
1112	16	0.1790957589137534
1112	17	0.31686164534189487
1112	18	0.20109494940880523
1112	19	0.19832697164476798
1112	20	-0.07652324221627538
1112	21	-0.0733344744862734
1112	22	-0.0583071231834964
1112	23	0.155963312208101
1112	24	0.29141895741290824
1112	25	0.13830237207846352
1113	1	0.00350174620484367
1113	2	0.19489734898106192
1113	3	0.1498724714234818
1113	4	0.14678363798282135
1113	5	0.04944496244333152
1113	6	-0.1662198233081753
1113	7	-0.07171605782702883
1113	8	-0.05518152852832259
1113	9	0.09614036016898098
1113	10	0.0776498354772528
1113	11	0.05735671115807861
1113	12	-0.08770920265003923
1113	13	0.1248576616420678
1113	14	0.353390657630392
1113	15	0.07790391145643127
1113	16	-0.05360223651447882
1113	17	-0.020864000301472174
1113	18	-0.03631632356265868
1113	19	-0.48205255285830617
1113	20	-0.2474570506251368
1113	21	-0.05191885482918934
1113	22	-0.5246479499391299
1113	23	-4.193840816042027
1113	24	-1.8733742986171364
1113	25	-1.1596368725505606
1114	1	-0.8793513215269257
1114	2	-1.1942868159955182
1114	3	-0.9867677976917051
1114	4	-1.0289638740792952
1114	5	-0.9488203402178002
1114	6	-1.6460691821220408
1114	7	-1.4078804418312174
1114	8	-1.340596064080561
1114	9	-1.531811884715136
1114	10	-1.241136538651098
1114	11	-1.4785702725638075
1114	12	-2.422278034309752
1114	13	-1.492106859153413
1114	14	-3.3537934699639536
1114	15	-2.113613943523106
1114	16	-2.073375709386628
1114	17	-1.8883380848347109
1114	18	-1.8757591528534405
1114	19	-1.9529917861855388
1114	20	-1.446139949797626
1114	21	-1.329790485610836
1114	22	-1.6453948839610995
1114	23	-5.474752291904295
1114	24	-2.922257804258738
1114	25	-2.750792996695587
1115	1	-2.1073032717028006
1115	2	-1.9244506356864193
1115	3	-1.7692755097363033
1115	4	-1.8999540010230795
1115	5	-1.638986836403852
1115	6	-1.728895816976138
1115	7	-2.3116167684381708
1115	8	-2.6368227784885363
1115	9	-4.106667245637251
1115	10	-4.658135830569936
1115	11	-3.520386018550944
1115	12	-3.631243147924387
1115	13	-3.1155925876748505
1115	14	-3.8403261749047246
1115	15	-3.564573849872719
1115	16	-3.6275580642555276
1115	17	-3.5316157315757444
1115	18	-6.098488681679147
1115	19	-4.112187562263038
1115	20	-4.457391445923616
1115	21	-3.9642330038918914
1115	22	-3.7220665903873034
1115	23	-4.177358877092789
1115	24	-3.937773392766019
1115	25	-4.133377994783082
1116	1	-2.4549051356521936
1116	2	-2.2306181580224766
1116	3	-2.637162334871025
1116	4	-2.542251507183469
1116	5	-2.5252572635913886
1116	6	-5.161057231586014
1116	7	-3.5261254742097385
1116	8	-3.866125005936457
1116	9	-5.034168621675139
1116	10	-4.336414044794683
1116	11	-3.937784618508747
1116	12	-3.7489144460687918
1116	13	-5.013467628576276
1116	14	-8.309393800687136
1116	15	-6.1853414396321575
1116	16	-4.99027042561579
1116	17	-5.7595351523818366
1116	18	-4.638946176718971
1116	19	-4.414764674039578
1116	20	-5.155869599196571
1116	21	-5.779201720698175
1116	22	-5.510250197260788
1116	23	-4.52634585896541
1116	24	-5.483737350643906
1116	25	-5.540886291457841
1201	1	0.9159276716196949
1201	2	0.881394956991727
1201	3	1.1888813589462153
1201	4	1.0372015485442176
1201	5	0.7010971813738749
1201	6	1.0891482347124324
1201	7	1.2906063273294102
1201	8	1.3532974003629334
1201	9	1.736082204422729
1201	10	1.4787136925993138
1201	11	1.651898578822589
1201	12	1.5405879312452129
1201	13	1.6171083204264818
1201	14	1.7541231002764164
1201	15	1.6926580176175463
1201	16	1.6141720000426574
1201	17	1.9721256199779518
1201	18	1.6869932722782224
1201	19	1.9730611273031755
1201	20	2.005888974637543
1201	21	2.069271782010013
1201	22	1.9766405933503108
1201	23	2.2343148064890173
1201	24	1.9918665799904787
1201	25	2.0182789324701886
1202	1	0.7892728826437505
1202	2	0.8613690090127868
1202	3	0.9632751830850985
1202	4	1.1424754853530175
1202	5	1.0449457347639637
1202	6	1.2428683193762922
1202	7	1.2436790240427307
1202	8	1.2070338286216113
1202	9	1.420276535818374
1202	10	1.4550013560100368
1202	11	1.362337495141361
1202	12	1.5164675092172786
1202	13	1.4483168182886956
1202	14	2.0301815498140527
1202	15	1.6616813566745066
1202	16	1.3056488153418755
1202	17	1.7170555745588985
1202	18	1.6866477363999977
1202	19	1.8174771666246485
1202	20	1.5808959862414937
1202	21	1.766322065803422
1202	22	1.7589232285834626
1202	23	1.7746466621730548
1202	24	1.7952506120965595
1202	25	2.074806079988285
1203	1	1.242483954666703
1203	2	1.3685368503390407
1203	3	1.2445547762458808
1203	4	1.3999576438252745
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2211	25	0.5627014393936491
2212	1	0.2664567567293936
2212	2	0.48609022912837574
2212	3	0.09933565911665099
2212	4	0.4503222812685621
2212	5	0.22911178379261798
2212	6	0.24280294365733823
2212	7	0.27760432668535273
2212	8	0.3458884845526953
2212	9	0.42899317794807956
2212	10	0.11974792697532166
2212	11	0.0848109463388127
2212	12	0.5480321825385417
2212	13	0.5767959984699615
2212	14	0.6840999512850203
2212	15	0.3477756884190768
2212	16	0.453549279545598
2212	17	0.49510874103124536
2212	18	0.7577434093701854
2212	19	0.49239971505927116
2212	20	0.5938746918167084
2212	21	0.7584861297218524
2212	22	0.8750101583037235
2212	23	0.8949489411971072
2212	24	0.8883456358846544
2212	25	0.9272310496120539
2213	1	-0.5976653933666718
2213	2	-0.5046093414744952
2213	3	-0.7280405986892688
2213	4	-0.430688265406598
2213	5	-0.5113542456191951
2213	6	-0.273637767360853
2213	7	-0.5657999401303897
2213	8	-0.42970588080929417
2213	9	-1.0067016837300713
2213	10	-0.5141013964393082
2213	11	-0.6512468090818189
2213	12	-0.6875377081915417
2213	13	-0.6943764112144564
2213	14	-0.853306991599311
2213	15	-0.7916486624195471
2213	16	-0.7766386860430572
2213	17	-0.7388501744232059
2213	18	-0.6928627586111347
2213	19	-0.7416551453857991
2213	20	-0.5383721424315624
2213	21	-0.7385099607795595
2213	22	-0.9249670504313556
2213	23	-0.7392994025395012
2213	24	-0.5647817724818154
2213	25	-0.4537381056321408
2214	1	-3.1977792459591896
2214	2	-1.775565861380251
2214	3	-1.930452235548696
2214	4	-3.6980886487806046
2214	5	-2.18169300979147
2214	6	-2.0812237234327284
2214	7	-1.7447800809351222
2214	8	-1.5894147133458354
2214	9	-1.5119309965558827
2214	10	-1.5917284835172154
2214	11	-1.6071682859326728
2214	12	-1.6101941984042618
2214	13	-1.963132835188862
2214	14	-1.7632762970809246
2214	15	-2.1990055077188195
2214	16	-1.9853505177520567
2214	17	-3.8148025039210403
2214	18	-3.60290199207396
2214	19	-2.62114182480817
2214	20	-2.557425584627655
2214	21	-2.53538428543866
2214	22	-3.4826876793615114
2214	23	-2.45310139390568
2214	24	-3.3729826731120482
2214	25	-3.3395485234957505
2215	1	-2.2097008529397897
2215	2	-3.771167265268687
2215	3	-2.341164167906797
2215	4	-3.8722765922643845
2215	5	-2.5258901784314887
2215	6	-2.405768187992173
2215	7	-2.6697171348502664
2215	8	-1.9502019035574598
2215	9	-4.307008369952754
2215	10	-2.750128924660025
2215	11	-2.401784054707253
2215	12	-2.4115415567495617
2215	13	-2.4403297055719477
2215	14	-2.070068578468998
2215	15	-2.2062538447630224
2215	16	-2.3119304124604234
2215	17	-2.6592639420743467
2215	18	-2.4422431217047453
2215	19	-2.440236857789838
2215	20	-2.3840311433045045
2215	21	-2.952600299754991
2215	22	-3.0055334666557663
2215	23	-2.928408841311885
2215	24	-2.830834113415577
2215	25	-3.733422118046665
2216	1	-4.381665122263744
2216	2	-4.562848435832791
2216	3	-5.779479308590272
2216	4	-6.435445178640936
2216	5	-5.966482214536235
2216	6	-5.233088248744021
2216	7	-6.770688939273651
2216	8	-4.7364828618018215
2216	9	-5.608413646080983
2216	10	-4.584902689837161
2216	11	-4.855069382304837
2216	12	-5.005838537687545
2216	13	-4.69987370303658
2216	14	-5.905864110749047
2216	15	-5.21912204892941
2216	16	-5.470253225056215
2216	17	-5.3331968448800495
2216	18	-4.8779246143852575
2216	19	-5.531756883559151
2216	20	-6.044745583644563
2216	21	-5.792274650456633
2216	22	-5.5144514152937845
2216	23	-5.515134787788607
2216	24	-6.063230278498505
2216	25	-6.0230194898538585
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	114522.0
3	3.0
4	921.0
5	6106.0
6	7908.0
7	7306.0
8	6270.0
9	5709.0
10	4954.0
11	4625.0
12	4223.0
13	4191.0
14	3968.0
15	4095.0
16	4195.0
17	4484.0
18	5097.0
19	6082.0
20	7867.0
21	10388.0
22	12873.0
23	15183.0
24	17466.0
25	19739.0
26	21483.0
27	23521.0
28	26130.0
29	30259.0
30	36694.0
31	45053.0
32	55382.0
33	70672.0
34	90572.0
35	118947.0
36	160976.0
37	246157.0
38	672945.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.1048051998732	18.28310955325417	12.460889493072234	24.151195753800394
2	15.117995279123175	19.982629730867394	41.58389147311605	23.315483516893387
3	19.5340136072545	25.57730127936325	28.73001977309165	26.158665340290593
4	12.51553205411016	16.26176759571262	37.56838899296875	33.65431135720847
5	13.205949662847111	38.954679511601206	34.18162829793073	13.657742527620961
6	31.94830207210815	36.14205359508978	18.147489139841305	13.762155192960773
7	28.654074918113466	32.074780547289436	21.16274345347624	18.108401081120856
8	26.13960056980695	36.246580704962774	20.193213836005036	17.420604889225242
9	28.07429715877461	14.593460126651125	19.622872706874524	37.70937000769974
10	16.93830755037941	28.349730972073356	32.54272718061932	22.169234296927907
11	34.586536328578255	22.473242014196984	24.26860794485018	18.671613712374583
12	24.775176666903093	24.685210003098437	30.465314837993347	20.074298492005127
13	29.665404713661086	22.16343180121574	25.44843766663112	22.722725818492055
14	21.75247770782831	22.687997047973628	26.898207347858126	28.661317896339934
15	23.584557049366868	31.18813544228943	23.14528302993494	22.08202447840876
16	22.130156248500278	27.873896336995944	27.71323265146805	22.282714763035727
17	21.667800811464605	28.626739081278718	27.36815833372442	22.33730177353226
18	21.56800211618315	26.633028883126748	30.399653560339484	21.399315440350623
19	23.79157772026666	26.295799952324906	27.81079067594642	22.10183165146201
20	24.45168030144585	26.33219729430379	28.265507839309223	20.950614564941137
21	23.743987407748264	26.050114133277596	27.258151291510945	22.94774716746319
22	24.042039851142192	27.58807900258016	27.070127216041147	21.299753930236502
23	22.59416970411235	27.38399268346661	28.11463280014718	21.907204812273857
24	23.6722936077267	26.04406632879424	28.598000068257058	21.685639995222004
25	22.962725963845784	26.733695941982617	28.462912600650565	21.840665493521037
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	433.0
1	433.0
2	960.5
3	1488.0
4	1488.0
5	1488.0
6	2776.5
7	4065.0
8	4065.0
9	4065.0
10	4581.0
11	5097.0
12	5097.0
13	5097.0
14	6362.5
15	7628.0
16	7628.0
17	7628.0
18	13645.0
19	19662.0
20	19662.0
21	19662.0
22	31579.5
23	43497.0
24	43497.0
25	43497.0
26	65278.5
27	87060.0
28	87060.0
29	87060.0
30	112781.5
31	138503.0
32	138503.0
33	138503.0
34	153161.5
35	167820.0
36	167820.0
37	167820.0
38	179030.5
39	190241.0
40	190241.0
41	190241.0
42	202850.0
43	215459.0
44	215459.0
45	215459.0
46	231760.0
47	248061.0
48	248061.0
49	248061.0
50	240895.5
51	233730.0
52	233730.0
53	233730.0
54	209887.5
55	186045.0
56	186045.0
57	186045.0
58	161441.0
59	136837.0
60	136837.0
61	136837.0
62	115933.0
63	95029.0
64	95029.0
65	95029.0
66	74124.0
67	53219.0
68	53219.0
69	53219.0
70	39559.5
71	25900.0
72	25900.0
73	25900.0
74	18495.0
75	11090.0
76	11090.0
77	11090.0
78	7667.5
79	4245.0
80	4245.0
81	4245.0
82	2791.0
83	1337.0
84	1337.0
85	1337.0
86	855.5
87	374.0
88	374.0
89	374.0
90	242.0
91	110.0
92	110.0
93	110.0
94	68.0
95	26.0
96	26.0
97	26.0
98	18.0
99	10.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3359144491695641
2	0.010442384145477329
3	0.009217002332487642
4	0.010762048966257248
5	0.011774320898726988
6	0.0816743617092691
7	0.08018259254562948
8	0.1036246794028235
9	0.0840718478651185
10	0.09035858934045689
11	0.08726849607291767
12	0.0973379379274851
13	0.08343251822355865
14	0.0875881608936976
15	0.08519067473784821
16	0.08630950161057792
17	0.09792399009891495
18	0.10080097348593423
19	0.09446095454046584
20	0.09254296561578633
21	0.08135469688848919
22	0.10058786360541427
23	0.093821624898906
24	0.09067825416123682
25	0.08876026523655729
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1876966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.86282469756607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.37458350047416	40.59887977738096
2	13.773925323917794	14.838050502391
3	4.701298339000486	7.596756248536252
4	2.161295667168315	4.656539585611841
5	1.1551096278086275	3.1108733694563444
6	0.6883024150606298	2.224434739279324
7	0.45407861877314815	1.7120569929323708
8	0.30627104693160834	1.3197298968653801
9	0.22150848507764376	1.07379654306845
>10	1.047473515823353	9.872279369426451
>50	0.0642894989787847	2.4402670668512614
>100	0.04325447524282077	4.440745621843617
>500	0.0059065076606296665	2.2517925350108285
>1k	0.0024026471839849487	2.64510131734725
>5k	2.0022059866541241E-4	0.6465815202901064
>10k+	1.0011029933270621E-4	0.5721149137084505
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	10610	0.5652739580791554	No Hit
TATCAACGCAGAGTACTTTTTTTTT	6145	0.32739005394876625	No Hit
GGTATCAACGCAGAGTACTTTTTTT	5846	0.31146009037990036	No Hit
GTCCTACAGTGGACATTTCTAAATT	4777	0.2545064748109449	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4097	0.21827779512255419	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4059	0.2162532512576147	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3673	0.19568814778743995	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	2954	0.15738164676397973	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2439	0.12994374964703675	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2387	0.12717332120027747	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2308	0.12296440106000855	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1967	0.10479678374568319	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1882	0.10026819878463435	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	5.327747012998637E-5	0.0	0.0	0.0	0.0
6	5.327747012998637E-5	0.0	0.0	0.0	5.327747012998637E-5
7	5.327747012998637E-5	0.0	0.0	0.0	5.327747012998637E-5
8	5.327747012998637E-5	0.0	0.0	0.0	5.327747012998637E-5
9	5.327747012998637E-5	0.0	0.0	0.0	5.327747012998637E-5
10	5.327747012998637E-5	0.0	0.0	0.0	5.327747012998637E-5
11	5.327747012998637E-5	0.0	0.0	0.0	5.327747012998637E-5
12	5.327747012998637E-5	0.0	0.0	0.0	5.327747012998637E-5
13	5.327747012998637E-5	0.0	0.0	0.0	5.327747012998637E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATTTA	35	0.0021718696	16.284092	14
GGTCTAC	180	0.0	12.163304	1
GGTATCA	1960	0.0	12.093152	1
TAGGACC	615	0.0	11.892807	4
CCCGTTT	130	2.6193447E-9	11.691142	12
TCCAACG	195	0.0	11.691142	18
TATGACT	115	7.088238E-8	11.563756	4
GTCCTAT	215	0.0	11.511478	1
AATGTCC	575	0.0	11.399168	8
TAGAAAT	590	0.0	11.269762	4
CCTAATA	135	4.7384674E-9	11.257836	2
GTCCTAA	1210	0.0	11.171185	1
GTCCTAC	2190	0.0	11.127358	1
AATAACG	60	0.0058796187	11.082524	7
CCGTTTC	140	8.365532E-9	10.856061	13
GTATCAA	4315	0.0	10.831708	1
TGTAGGA	2305	0.0	10.796901	2
TCCTACA	2325	0.0	10.785734	2
AAATGTC	620	0.0	10.725024	7
GGACGTG	2420	0.0	10.716161	6
>>END_MODULE