FastQCFastQC Report
Thu 26 May 2016
SRR1512872_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512872_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876966
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT129580.6903694579443633No Hit
TATCAACGCAGAGTACTTTTTTTTT95760.5101850539647494No Hit
GGTATCAACGCAGAGTACTTTTTTT76400.40703987179309586No Hit
GTCCTACAGTGGACATTTCTAAATT45260.2411338298083183No Hit
ACGCAGAGTACTTTTTTTTTTTTTT44380.2364454124368795No Hit
CTGTAGGACGTGGAATATGGCAAGA41690.22211377297191318No Hit
GTCCTAAAGTGTGTATTTCTCATTT39440.21012634219266624No Hit
CTTTAGGACGTGAAATATGGCGAGG38950.2075157461562969No Hit
GTACTTTTTTTTTTTTTTTTTTTTT28140.14992280094578164No Hit
GAGTACTTTTTTTTTTTTTTTTTTT22660.12072674731454913No Hit
GTACTGGTTCACTATCGGTCAGTCA21890.11662438211454017No Hit
GTCCTACAGTGTGCATTTCTCATTT21770.11598505247298033No Hit
CTGAAGGACCTGGAATATGGCGAGA19650.10469022880542321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22950.013.2185381
AGCACCG1207.2941475E-1012.6798525
TCTATAC1301.9463187E-1012.4356763
TAGGACC6450.012.2375314
AGCGCAT701.0927627E-412.21233310
GGCGAGG10850.012.07232719
GCACCGT951.0271524E-612.012496
ATAGGAC2100.011.7738333
TAGAAAT4800.011.4911164
CTAAGAC1451.196895E-911.1492273
CCGATAG600.005832251611.0945743
AATCCCG1803.6379788E-1211.0735919
TTAATAC1553.074092E-1011.04344753
GACGTGG11450.011.0425897
TCCAACG1553.1832315E-1011.02314718
GTATAGA1900.011.011451
TGGCGAG22900.010.9014718
AATGTCC5600.010.8583018
CGCCACC1408.443749E-910.84962314
GGACCGT803.7353724E-410.6986256
Produced by FastQC (version 0.11.5)