##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1512872_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1876966
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.955965638162866	31.0	30.0	34.0	26.0	34.0
2	29.967540701323305	31.0	30.0	34.0	26.0	34.0
3	30.129910184840856	31.0	30.0	34.0	26.0	34.0
4	33.69010733279132	37.0	35.0	37.0	29.0	37.0
5	33.23291950946368	37.0	35.0	37.0	27.0	37.0
6	33.05001209398572	37.0	35.0	37.0	26.0	37.0
7	32.86796724074917	37.0	35.0	37.0	25.0	37.0
8	32.79714283583187	37.0	35.0	37.0	25.0	37.0
9	34.05240585071866	39.0	35.0	39.0	23.0	39.0
10	33.979879763405414	39.0	35.0	39.0	23.0	39.0
11	33.93870373784075	39.0	35.0	39.0	23.0	39.0
12	33.800273419976705	39.0	34.0	39.0	22.0	39.0
13	33.77771520634897	39.0	34.0	39.0	21.0	39.0
14	34.83759002560515	39.0	35.0	41.0	20.0	41.0
15	34.83347807046052	39.0	35.0	41.0	21.0	41.0
16	34.842210247814826	39.0	35.0	41.0	21.0	41.0
17	34.844877317969534	39.0	35.0	41.0	21.0	41.0
18	34.769289907222614	40.0	35.0	41.0	19.0	41.0
19	34.787845917294185	40.0	35.0	41.0	19.0	41.0
20	34.75873191096695	40.0	35.0	41.0	18.0	41.0
21	34.72504616492787	40.0	35.0	41.0	18.0	41.0
22	34.690587895571895	40.0	35.0	41.0	18.0	41.0
23	34.52938412310079	39.0	34.0	41.0	18.0	41.0
24	34.480583026011125	39.0	34.0	41.0	17.0	41.0
25	34.416285111184756	39.0	34.0	41.0	16.0	41.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7101936421416042
1101	2	0.5786797534938053
1101	3	0.4220548004698692
1101	4	0.5686031421127566
1101	5	0.6460621039374672
1101	6	0.6042085132214225
1101	7	0.8490444845101806
1101	8	0.4878415552018396
1101	9	0.9019762455359697
1101	10	0.6218356531570777
1101	11	0.954351677976689
1101	12	1.015486092338044
1101	13	1.0502689358578507
1101	14	1.1281092859124726
1101	15	0.8747784658229847
1101	16	1.011794575976765
1101	17	1.1400410797880838
1101	18	0.915047468554441
1101	19	0.8989054403989769
1101	20	0.9186682099194883
1101	21	0.94264413637827
1101	22	0.8115587919917004
1101	23	0.9702644828440228
1101	24	0.984672252104005
1101	25	1.0751916740901848
1102	1	0.775249674099264
1102	2	0.8216342208998206
1102	3	0.5194369035747286
1102	4	0.6077334725977792
1102	5	0.8225132786137479
1102	6	0.8402522883461643
1102	7	0.7902970348606644
1102	8	0.8317373796973229
1102	9	0.9204954514426831
1102	10	0.9200048297726369
1102	11	0.9392898382902075
1102	12	0.9085981259521674
1102	13	0.9727909294743995
1102	14	0.9139983986935221
1102	15	1.1201348078628612
1102	16	1.151913735421651
1102	17	1.1767603190261084
1102	18	0.6887172461436748
1102	19	1.025123548392429
1102	20	1.2135368289930781
1102	21	1.1203371549832042
1102	22	0.866013067490158
1102	23	1.0050967152339751
1102	24	1.0367486414137446
1102	25	1.0608994847279263
1103	1	0.6054138533297646
1103	2	0.7769802686446035
1103	3	0.508712601888309
1103	4	0.39168646361260784
1103	5	0.5766710745036576
1103	6	0.5893328436437173
1103	7	0.7659310566667941
1103	8	0.9015192822271345
1103	9	0.9776782173880676
1103	10	0.906199830454149
1103	11	1.0710206620330496
1103	12	1.2022075480137744
1103	13	1.1001680061408337
1103	14	1.001074667068842
1103	15	1.0900087453297687
1103	16	1.1292100553510167
1103	17	1.194169879321052
1103	18	1.0744237258616138
1103	19	1.0373482728605765
1103	20	1.0151507575098009
1103	21	1.1624465764416954
1103	22	1.0863144306445776
1103	23	1.163473643534779
1103	24	1.0795249844543733
1103	25	1.1004233637532437
1104	1	0.6315239119240701
1104	2	0.7830708430729842
1104	3	0.7175954340786852
1104	4	0.5531755511561656
1104	5	0.572305335627405
1104	6	0.5754372583529914
1104	7	0.3790744075651844
1104	8	0.48796029748358904
1104	9	0.5694414540474213
1104	10	0.6486328175913911
1104	11	0.7319444156987416
1104	12	0.7832143150035691
1104	13	0.5949783734944063
1104	14	0.796387879053924
1104	15	0.8074753405261319
1104	16	0.8940467608347547
1104	17	0.8586990545197253
1104	18	0.46106010273322084
1104	19	0.851254162732026
1104	20	0.7799683191623856
1104	21	0.6018953475495437
1104	22	0.6283275770206771
1104	23	0.5640115142869746
1104	24	0.7795390232638795
1104	25	0.6622149238289552
1105	1	0.4785627622530093
1105	2	0.5086196859916221
1105	3	0.5294940544055287
1105	4	0.2952554409633308
1105	5	0.4754878043316211
1105	6	0.5474718661445479
1105	7	0.5707720447546976
1105	8	0.4707801423764195
1105	9	0.5993810403151727
1105	10	0.4119423644318303
1105	11	0.6219248151760581
1105	12	0.741839676326336
1105	13	0.7982708653345938
1105	14	0.7731925130360793
1105	15	0.9683049828519472
1105	16	0.9505418027227392
1105	17	0.7312560600802769
1105	18	0.5384779247842317
1105	19	0.8902345961496465
1105	20	0.89590908768389
1105	21	0.8077828580851758
1105	22	0.7213572295285786
1105	23	1.0365983590270815
1105	24	0.9916835693406654
1105	25	0.7816847763583539
1106	1	0.23758570951622104
1106	2	0.46675496329374866
1106	3	-1.5622805513179046
1106	4	-0.2663362545384018
1106	5	0.04690378949607066
1106	6	0.2616350335252804
1106	7	0.24370403189911372
1106	8	0.2782872060788222
1106	9	0.5521256409939497
1106	10	0.29916209590456333
1106	11	0.38132315115014137
1106	12	0.501025805180582
1106	13	0.46952106368667046
1106	14	0.6268929931073188
1106	15	0.6010312996137017
1106	16	0.5666515441189972
1106	17	0.5771811386513832
1106	18	0.7128669411710717
1106	19	0.7278667815277231
1106	20	0.7463869089609716
1106	21	0.6665926508753444
1106	22	0.5905410136836267
1106	23	0.6891444892076208
1106	24	0.6421609169411369
1106	25	0.6904103731316695
1107	1	0.7414188289088983
1107	2	0.822624847535959
1107	3	0.7989886817939009
1107	4	0.6273710171838545
1107	5	0.7886530158046838
1107	6	0.7054145317664506
1107	7	0.7573915605570107
1107	8	0.6995466251178897
1107	9	0.7057883931403524
1107	10	0.7675261911079616
1107	11	0.8653982667298052
1107	12	0.98853653573142
1107	13	0.9043479211493306
1107	14	0.9189362393819351
1107	15	0.8796556975824004
1107	16	1.0751309489311112
1107	17	1.01459849760856
1107	18	1.0384613303714545
1107	19	0.8957832676759736
1107	20	0.955405513179187
1107	21	0.9854806038137625
1107	22	0.9625482673636157
1107	23	1.0862663457451802
1107	24	1.0925919202174796
1107	25	1.080896928196772
1108	1	0.6754004898915582
1108	2	0.6526330989988764
1108	3	0.5684188530839194
1108	4	0.4447725904804827
1108	5	0.6412515409372475
1108	6	0.6279075153686975
1108	7	0.5736374203100141
1108	8	0.5690118217169342
1108	9	0.7324980943767869
1108	10	0.6550090171494034
1108	11	0.6987692575235798
1108	12	0.7056479419826474
1108	13	0.725718845143966
1108	14	0.8184415829258072
1108	15	0.9099372725335186
1108	16	0.830587492830027
1108	17	0.7739050606348172
1108	18	0.8818665689942335
1108	19	0.9726176477318731
1108	20	0.9327435742103702
1108	21	1.0743622745619206
1108	22	1.0572584945879555
1108	23	1.082307612128531
1108	24	1.0295806483055046
1108	25	1.0200237816378106
1109	1	0.32848798259225376
1109	2	0.2316852572440986
1109	3	0.41899475267615927
1109	4	0.19288798514472205
1109	5	0.09393146077288606
1109	6	0.4134404219419565
1109	7	0.296164309892589
1109	8	0.4718060376155151
1109	9	0.5588440280918405
1109	10	0.2287353908090921
1109	11	0.5272587629053831
1109	12	0.6966820273815202
1109	13	0.5766938121576146
1109	14	0.5825980666554855
1109	15	0.5648518810993437
1109	16	0.6801506228199656
1109	17	0.6904225766855134
1109	18	0.8020888386663145
1109	19	0.7612719188803112
1109	20	0.7069624641344987
1109	21	0.7150129432219288
1109	22	0.7411818847467586
1109	23	0.7497101218118019
1109	24	0.7105198047364922
1109	25	0.7175414468113601
1110	1	0.30346384185282105
1110	2	0.10671961573040534
1110	3	0.24191241288838583
1110	4	0.20410529180853842
1110	5	0.30596656296085456
1110	6	0.3266658336165804
1110	7	0.2894135235571156
1110	8	0.3014680738947604
1110	9	0.14242845790343495
1110	10	0.17734283182660704
1110	11	0.21741688986030994
1110	12	0.40091168829047774
1110	13	0.32921279898594946
1110	14	0.28099758840524913
1110	15	0.3157008824734646
1110	16	0.2353142432644404
1110	17	0.30283243642994506
1110	18	0.2356574030634846
1110	19	0.3285640988059342
1110	20	0.2303608834286095
1110	21	0.3449656078102663
1110	22	0.4451266202751043
1110	23	0.4847304020223149
1110	24	0.38010174360618265
1110	25	0.3234728461077552
1111	1	0.06026060837037761
1111	2	-0.13265109326588842
1111	3	-0.1699596600723332
1111	4	0.013069072836891849
1111	5	0.15870620788154355
1111	6	0.10933257697156762
1111	7	0.03172296334781066
1111	8	0.07392497463798975
1111	9	0.07616046866148451
1111	10	0.08136863053569243
1111	11	0.025262432880765573
1111	12	0.2173550911609894
1111	13	0.1450048604128611
1111	14	0.019628607952149935
1111	15	0.1716245480502323
1111	16	0.18253397681471029
1111	17	0.28908245266436694
1111	18	0.14286558534391958
1111	19	-0.13406011090505388
1111	20	0.13475186117091198
1111	21	0.28010922215938194
1111	22	0.060474464218152946
1111	23	0.10647913811105525
1111	24	0.2405913476270385
1111	25	0.23873581266462196
1112	1	0.20496105807873377
1112	2	-0.2353673126066873
1112	3	0.13074922657309074
1112	4	0.21965293188442558
1112	5	0.15418781782202018
1112	6	0.04313177608652552
1112	7	0.2223406484123842
1112	8	0.1937661803768691
1112	9	0.19044644073714778
1112	10	-0.11717159194107296
1112	11	0.06814523204977263
1112	12	0.3680041837380301
1112	13	0.21747172745479304
1112	14	-0.05530698610173346
1112	15	-0.31194385063515995
1112	16	-0.29765908176847233
1112	17	-0.14723531544117918
1112	18	0.05061770341060168
1112	19	0.12592499846411442
1112	20	0.018337522465259326
1112	21	-0.05225502802104387
1112	22	0.1566112162582769
1112	23	0.2501812263540728
1112	24	0.2339756810267204
1112	25	0.17351978491922182
1113	1	-0.5499958615888509
1113	2	-0.39765983688445417
1113	3	-0.39635886419546296
1113	4	-1.7514423575690259
1113	5	-0.6301014112028938
1113	6	-0.3252224100761012
1113	7	-0.2854944418238503
1113	8	-0.3136266649909345
1113	9	-0.1617599713289124
1113	10	-0.4242916021778953
1113	11	-0.32781512096206455
1113	12	-0.042971461740158645
1113	13	-0.28675867066910143
1113	14	-0.3525452021370299
1113	15	-0.558754809233946
1113	16	-0.8006500710292812
1113	17	-0.6943087357977902
1113	18	-0.5546133398005537
1113	19	-0.3919695107178569
1113	20	-0.18213198829694477
1113	21	-0.2167854258586246
1113	22	-0.2953573129978295
1113	23	-0.11710027373699461
1113	24	0.11152549909625264
1113	25	-0.29074361418629024
1114	1	-1.046819179059348
1114	2	-2.201338595166522
1114	3	-1.5275481339242276
1114	4	-1.1049520996800481
1114	5	-0.9244910296479389
1114	6	-0.7907126054305991
1114	7	-0.8608046509359326
1114	8	-0.9161366053201156
1114	9	-1.1810540516208832
1114	10	-1.253690031985478
1114	11	-1.1729288587265927
1114	12	-1.3088403945395868
1114	13	-1.3935928359110505
1114	14	-1.364627164307592
1114	15	-1.4039715789055975
1114	16	-1.798082465137746
1114	17	-1.7474339716000955
1114	18	-1.598277177493344
1114	19	-1.913277732110238
1114	20	-1.493853234953022
1114	21	-1.6386373972755237
1114	22	-1.370823512186547
1114	23	-1.2818834832974346
1114	24	-1.344399689355221
1114	25	-1.794243468464792
1115	1	-1.2950257083827417
1115	2	-1.4808494522051951
1115	3	-1.018112758764147
1115	4	-0.7644654288841224
1115	5	-1.1776215690751002
1115	6	-1.2259896423259278
1115	7	-0.9837250484448603
1115	8	-1.11448611494556
1115	9	-2.178122930168289
1115	10	-1.938890753965552
1115	11	-2.107293877653479
1115	12	-2.6971981570659835
1115	13	-2.5704322611337176
1115	14	-2.2898846899574252
1115	15	-2.3926377895352147
1115	16	-2.3165649328034092
1115	17	-2.1441094143416777
1115	18	-2.520132276042588
1115	19	-2.907906891020513
1115	20	-3.088745485253373
1115	21	-2.424362629226273
1115	22	-2.4828849063149363
1115	23	-2.4271154542565654
1115	24	-2.887544420718296
1115	25	-2.8881819366944903
1116	1	-1.6988868030122148
1116	2	-1.9486570994429009
1116	3	-1.9633406725718423
1116	4	-1.5457075973547418
1116	5	-2.0384956362666955
1116	6	-2.755619774567542
1116	7	-2.2120238495832005
1116	8	-2.655478506537385
1116	9	-3.5461202015355084
1116	10	-2.2832165416254924
1116	11	-3.3647640216760237
1116	12	-5.387773048691649
1116	13	-4.389575152143923
1116	14	-3.785993317541646
1116	15	-4.77141223587601
1116	16	-4.336812418211792
1116	17	-4.0872050059686345
1116	18	-4.134044274993929
1116	19	-4.374669178700426
1116	20	-6.039818959898451
1116	21	-4.993557936796741
1116	22	-4.236514195929395
1116	23	-4.872154338264245
1116	24	-4.765657345304817
1116	25	-4.1291627388610195
1201	1	0.6182739502400523
1201	2	0.8000035602067115
1201	3	0.8705361366562698
1201	4	0.7064537291231048
1201	5	0.2705158809719137
1201	6	0.8308162328993873
1201	7	0.9914129400826823
1201	8	0.8934136443355882
1201	9	0.8757826509994331
1201	10	0.9433893244312799
1201	11	0.575495835748022
1201	12	1.1147183057605758
1201	13	1.3163671412130924
1201	14	1.1827741120229618
1201	15	1.0414184874669417
1201	16	0.941600825669326
1201	17	0.9208888424260167
1201	18	1.1431514087384116
1201	19	1.4105329183860889
1201	20	1.4353833500313158
1201	21	1.0767659574220971
1201	22	1.073499861399938
1201	23	1.1091875320184315
1201	24	0.9180586705210487
1201	25	1.097523789950941
1202	1	0.8476638700757277
1202	2	0.8756749086743483
1202	3	0.8583319103237095
1202	4	0.8023275767483398
1202	5	0.8403090840541978
1202	6	0.8468173184369192
1202	7	0.8817327902865699
1202	8	0.8401375606644521
1202	9	1.0335946875525863
1202	10	1.0153293898460163
1202	11	0.9913792877573755
1202	12	0.9780545782506707
1202	13	0.909500410113651
1202	14	1.0367430834055256
1202	15	1.0022781000228633
1202	16	0.952313951190149
1202	17	0.9412764681052366
1202	18	0.9728012119090721
1202	19	0.9545375430181053
1202	20	0.9352567376146226
1202	21	1.0156182133426128
1202	22	0.8995581091648006
1202	23	1.079171309487272
1202	24	1.0011058228009801
1202	25	1.0879789288008794
1203	1	0.994918934410002
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2211	10	-0.12969570514058404
2211	11	0.030577058635614662
2211	12	-0.15605935935630555
2211	13	0.05512272911811067
2211	14	0.029907811104180837
2211	15	-0.06044875344596079
2211	16	-0.03080168643091241
2211	17	-0.13373834189262368
2211	18	-0.09400249145873119
2211	19	-0.03817999028267849
2211	20	0.0398934729700926
2211	21	0.015623037446154342
2211	22	0.022736286464109412
2211	23	-0.06738978006545437
2211	24	0.08139627242672276
2211	25	-0.1409807436231958
2212	1	-0.03142452030424181
2212	2	0.05185934092174804
2212	3	0.13412281204630716
2212	4	0.08858196462693257
2212	5	-0.10400320431750032
2212	6	0.09417158837434414
2212	7	-0.15170839492344612
2212	8	0.13163272141648008
2212	9	0.2234715404064147
2212	10	-0.08588679116213171
2212	11	0.05340338743860684
2212	12	0.27181585646516737
2212	13	0.22426150098999642
2212	14	0.42540016741248365
2212	15	0.2378116453517123
2212	16	0.23299033343511866
2212	17	0.1738010448004701
2212	18	0.08898903454366547
2212	19	0.36225796995646675
2212	20	0.26660821144579216
2212	21	0.3040651559501981
2212	22	0.25573417620970673
2212	23	-0.02156978388369879
2212	24	-0.045239662469860775
2212	25	-5.361827423797649E-4
2213	1	-0.7290665249518575
2213	2	-0.2816513347769103
2213	3	-0.27768451794315396
2213	4	-0.5870775532720032
2213	5	-0.5189975013056909
2213	6	-0.36870895734136155
2213	7	-0.8563100482946311
2213	8	-0.7792492762898391
2213	9	-0.6186871733903772
2213	10	-0.5307580811304078
2213	11	-0.5000641234054228
2213	12	-0.6712589761246761
2213	13	-0.5418301538394132
2213	14	-0.6567969798000419
2213	15	-0.9182416695823505
2213	16	-1.4395591120063003
2213	17	-1.123149393896746
2213	18	-0.9061704194496727
2213	19	-0.8371988497753691
2213	20	-0.7943120083894613
2213	21	-0.7629712972579128
2213	22	-0.7163378649697449
2213	23	-0.9603198180879176
2213	24	-0.7534498173292334
2213	25	-0.9704648751784148
2214	1	-1.5921457807635235
2214	2	-0.7754876576192906
2214	3	-0.930136477595763
2214	4	-0.9603576851161151
2214	5	-1.2848793230174493
2214	6	-1.9209850428502833
2214	7	-1.4539377678869378
2214	8	-1.255873828039185
2214	9	-0.9729563820621436
2214	10	-1.4053206359263584
2214	11	-1.387489561377727
2214	12	-1.0950418690698598
2214	13	-1.432140911679518
2214	14	-1.1031325251767967
2214	15	-1.2726531939947705
2214	16	-1.2061395107363495
2214	17	-1.3534963807562619
2214	18	-1.3094433300876887
2214	19	-1.249592266706017
2214	20	-1.6220660375940597
2214	21	-1.7182433926551397
2214	22	-2.129847318424595
2214	23	-2.105401052264071
2214	24	-1.9093878098339374
2214	25	-2.2649164939760382
2215	1	-1.7356486350129892
2215	2	-1.3344266952898352
2215	3	-1.430982348124509
2215	4	-1.482187664793031
2215	5	-2.0112077801967736
2215	6	-1.7842245292934642
2215	7	-2.2349408822441923
2215	8	-1.8966677463722732
2215	9	-1.9676213979113797
2215	10	-2.0147465953772894
2215	11	-1.7785064787883016
2215	12	-2.1364146139353295
2215	13	-2.166925993284
2215	14	-2.473678612808847
2215	15	-2.1470963899218276
2215	16	-2.1685258436327004
2215	17	-2.3111400816392376
2215	18	-2.066158966380705
2215	19	-2.2409355326887734
2215	20	-2.215579989775847
2215	21	-2.1343333136517373
2215	22	-2.24074236666592
2215	23	-2.71370126520565
2215	24	-2.519612126790882
2215	25	-2.8240330985636497
2216	1	-1.7828980033357844
2216	2	-1.200325391411102
2216	3	-1.2262176229885178
2216	4	-1.4185220493342143
2216	5	-2.3826205550564516
2216	6	-2.6656220469189478
2216	7	-2.559397397394921
2216	8	-2.6227497371209516
2216	9	-4.525410986624145
2216	10	-2.618590604445039
2216	11	-3.004575048019948
2216	12	-3.3024292546456486
2216	13	-3.126527227024482
2216	14	-3.2120218087924357
2216	15	-3.0731572526049895
2216	16	-2.3699901744358627
2216	17	-2.64254114947245
2216	18	-3.09456019527212
2216	19	-3.271116194967039
2216	20	-2.562448706557589
2216	21	-3.366348480831885
2216	22	-2.7883653727047353
2216	23	-3.7768508016870648
2216	24	-3.1458843567196517
2216	25	-3.263056548984565
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65535.0
3	3.0
4	1058.0
5	9260.0
6	12154.0
7	9332.0
8	7644.0
9	6145.0
10	5010.0
11	4593.0
12	3936.0
13	3555.0
14	3537.0
15	3479.0
16	3498.0
17	3622.0
18	4031.0
19	4889.0
20	6562.0
21	8629.0
22	10837.0
23	12867.0
24	14873.0
25	16754.0
26	18207.0
27	19487.0
28	22010.0
29	26369.0
30	32803.0
31	41208.0
32	52217.0
33	66624.0
34	87407.0
35	113844.0
36	157067.0
37	243363.0
38	774557.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.930219908845466	19.112376464916917	13.593938388835355	26.363465237402263
2	13.509684496331037	20.374246525984425	40.44435845242074	25.671710525263798
3	17.430056021259624	25.348594193036483	29.635635340126793	27.585714445577104
4	11.546892032867206	16.843217968486098	36.83564158931336	34.77424840933333
5	12.791247883214169	38.89317545050638	34.41507439256639	13.900502273713064
6	31.810820118541294	36.69784977305707	17.734654297201942	13.75667581119969
7	28.100900839418546	32.139163055347026	21.282864430492047	18.477071674742373
8	25.9715220961477	36.54356944454069	19.88911815297132	17.595790306340287
9	27.24797266548794	14.421065308467112	19.60069316775747	38.73026885828748
10	16.225149325251344	28.85135488080098	32.67459301340116	22.248902780546516
11	34.201115461092066	22.463839155369623	24.299267086225527	19.035778297312792
12	24.244390669603327	24.92069631670753	30.62731158116147	20.20760143252767
13	29.262010727588827	22.04702257993876	25.736729177666966	22.95423751480545
14	21.38648592059185	22.435308991502755	27.160127461359078	29.01807762654632
15	23.38469080281236	31.090069616324268	23.201285224448036	22.32395435641533
16	22.01656440017988	27.661663814287756	27.75943682744422	22.562334958088144
17	21.3165046867699	28.603282363966994	27.658895432257484	22.421317517005626
18	21.302212428044406	26.34587063870779	30.7813828687611	21.570534064486704
19	23.621558090890588	25.99222776671366	28.239931207104334	22.146282935291417
20	24.106611030424826	26.068459855702375	28.69271170204491	21.132217411827884
21	23.205293228852554	25.701345364109272	27.872191475169476	23.221169931868697
22	23.60167504901543	27.34453584519649	27.65903375671298	21.3947553490751
23	22.21851647818874	27.043057785809655	28.626144533252067	22.112281202749543
24	23.53345771846693	25.734083622186017	28.955399298655383	21.77705936069167
25	22.550979906391436	26.519247828275965	28.91790736641333	22.011864898919267
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	241.0
1	241.0
2	589.0
3	937.0
4	937.0
5	937.0
6	2255.5
7	3574.0
8	3574.0
9	3574.0
10	4139.5
11	4705.0
12	4705.0
13	4705.0
14	5852.0
15	6999.0
16	6999.0
17	6999.0
18	13647.0
19	20295.0
20	20295.0
21	20295.0
22	32746.0
23	45197.0
24	45197.0
25	45197.0
26	68281.0
27	91365.0
28	91365.0
29	91365.0
30	115839.0
31	140313.0
32	140313.0
33	140313.0
34	154777.5
35	169242.0
36	169242.0
37	169242.0
38	180288.5
39	191335.0
40	191335.0
41	191335.0
42	204325.0
43	217315.0
44	217315.0
45	217315.0
46	232821.0
47	248327.0
48	248327.0
49	248327.0
50	240599.0
51	232871.0
52	232871.0
53	232871.0
54	208343.5
55	183816.0
56	183816.0
57	183816.0
58	159671.0
59	135526.0
60	135526.0
61	135526.0
62	114194.5
63	92863.0
64	92863.0
65	92863.0
66	72080.5
67	51298.0
68	51298.0
69	51298.0
70	37944.5
71	24591.0
72	24591.0
73	24591.0
74	17606.5
75	10622.0
76	10622.0
77	10622.0
78	7277.0
79	3932.0
80	3932.0
81	3932.0
82	2569.0
83	1206.0
84	1206.0
85	1206.0
86	766.0
87	326.0
88	326.0
89	326.0
90	192.0
91	58.0
92	58.0
93	58.0
94	34.5
95	11.0
96	11.0
97	11.0
98	6.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08998564704954698
2	0.04384735791697879
3	0.019020056836405133
4	0.00426219761039891
5	0.015983241038995913
6	0.062014975231304136
7	0.07362946371964117
8	0.04874888516893753
9	0.019392999127315038
10	0.04581862431178828
11	0.003942532789618991
12	2.1310988051994546E-4
13	0.006553128825988324
14	2.6638735064993187E-4
15	0.013372645002626578
16	0.009057169922097683
17	0.004635139901308815
18	0.006073631594818446
19	0.0014917691636396183
20	6.393296415598365E-4
21	1.0655494025997273E-4
22	3.1966482077991826E-4
23	0.0
24	0.0
25	5.3277470129986365E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1876966.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.42494189924597
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36585971745315	38.986258749749574
2	14.170117169797484	14.857351386642815
3	5.028524913469545	7.908603792826553
4	2.2688440363467532	4.75776067135717
5	1.1959016359025594	3.134753688970244
6	0.7217404404424265	2.2702320393918245
7	0.46540230136908844	1.7079082026334875
8	0.3282803712633688	1.3768063512116004
9	0.23549993220377016	1.1111463236753112
>10	1.0951496315869165	10.016083028876158
>50	0.07035722214797763	2.5689253725363552
>100	0.045627909412220766	4.6328944014027265
>500	0.005523703584269478	2.0655622697886886
>1k	0.0028641425992508404	2.9876059290323336
>5k	2.045816142322029E-4	0.9232234289600204
>10k+	1.0229080711610145E-4	0.6948843629451641
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	12958	0.6903694579443633	No Hit
TATCAACGCAGAGTACTTTTTTTTT	9576	0.5101850539647494	No Hit
GGTATCAACGCAGAGTACTTTTTTT	7640	0.40703987179309586	No Hit
GTCCTACAGTGGACATTTCTAAATT	4526	0.2411338298083183	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	4438	0.2364454124368795	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4169	0.22211377297191318	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	3944	0.21012634219266624	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3895	0.2075157461562969	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2814	0.14992280094578164	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2266	0.12072674731454913	No Hit
GTACTGGTTCACTATCGGTCAGTCA	2189	0.11662438211454017	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2177	0.11598505247298033	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1965	0.10469022880542321	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2295	0.0	13.218538	1
AGCACCG	120	7.2941475E-10	12.679852	5
TCTATAC	130	1.9463187E-10	12.435676	3
TAGGACC	645	0.0	12.237531	4
AGCGCAT	70	1.0927627E-4	12.212333	10
GGCGAGG	1085	0.0	12.072327	19
GCACCGT	95	1.0271524E-6	12.01249	6
ATAGGAC	210	0.0	11.773833	3
TAGAAAT	480	0.0	11.491116	4
CTAAGAC	145	1.196895E-9	11.149227	3
CCGATAG	60	0.0058322516	11.094574	3
AATCCCG	180	3.6379788E-12	11.07359	19
TTAATAC	155	3.074092E-10	11.0434475	3
GACGTGG	1145	0.0	11.042589	7
TCCAACG	155	3.1832315E-10	11.023147	18
GTATAGA	190	0.0	11.01145	1
TGGCGAG	2290	0.0	10.90147	18
AATGTCC	560	0.0	10.858301	8
CGCCACC	140	8.443749E-9	10.849623	14
GGACCGT	80	3.7353724E-4	10.698625	6
>>END_MODULE